OsFBX148基因在调控植物耐冷性中的应用

By knocking out or reducing the expression of the rice OsFBX148 gene, gene editing technology was used to improve the cold tolerance of rice, solving the problem of rice's sensitivity to low temperature stress and achieving the effect of enhanced cold tolerance.

CN122128358BActive Publication Date: 2026-07-17SANYA INST OF HENAN UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SANYA INST OF HENAN UNIV
Filing Date
2026-04-30
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Rice is sensitive to low temperature stress, which leads to slow growth, pollen abortion and reduced yield. Current technologies lack effective cold-resistant gene resources and molecular mechanisms, which affects food security.

Method used

By knocking out or reducing the expression of the OsFBX148 gene, gene editing technology is used to knock out or reduce the expression level of the OsFBX148 gene in rice, thereby improving the cold tolerance of rice. Cold-tolerant plants are obtained by gene editing or mRNA interference methods, and then introduced into rice callus tissue through Agrobacterium-mediated genetic transformation to obtain transgenic rice with enhanced cold tolerance.

Benefits of technology

It significantly improved the rice's tolerance to low temperatures, manifested in less leaf damage, higher survival rate, higher proline content and total soluble sugar content, thus enhancing the plant's ability to adapt to low temperatures.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明属于生物技术领域,涉及一种OsFBX148基因在调控植物耐冷性中的应用。本发明揭示了水稻中一个名为OsFBX148的基因及其编码蛋白的新功能。组织特异性表达分析显示,该基因在水稻根中呈现高丰度表达,且其表达水平受低温胁迫的显著诱导上调。通过基因编辑技术(如CRISPR / Cas9)定向突变OsFBX148基因,能够显著增强水稻植株的耐冷性,说明该基因在调控水稻的耐冷性起着重要的作用,本发明为水稻的耐冷性遗传改良提供了重要的基因资源,对于培育耐冷性增强的作物品种,保障高寒地区粮食安全生产具有重要的应用价值。
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