High-efficiency and environment-friendly method for extracting high-purity alkaloids from piper longum
By combining nano-extraction and macroporous adsorption resin with chromatographic purification, the problems of low extraction efficiency and poor purity of Boluoride alkaloids have been solved, achieving efficient and environmentally friendly alkaloid extraction, simplifying the process steps and improving product quality.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GUIZHOU FITO TECHNOLOGY CO LTD
- Filing Date
- 2026-03-06
- Publication Date
- 2026-06-05
AI Technical Summary
Existing methods for extracting alkaloids from Boluohui are inefficient, produce poor purity, and suffer from problems such as high solvent consumption, numerous impurities, and high production costs.
The extraction rate and purity are improved by combining nano-extraction technology with macroporous adsorption resin and chromatographic purification, using a composite eluent composed of glycolic acid and sulfuric acid for one-time elution, and combining steps of pulverization, grinding, homogenization, purification, concentration, cooling crystallization, vacuum drying and spray drying.
This method achieves efficient and environmentally friendly extraction of alkaloids from *Boluocybe salvia*, simplifies the process, improves the extraction rate and product quality, reduces solvent usage and loss, and yields high-purity alkaloid products.
Smart Images
Figure CN122145479A_ABST
Abstract
Description
Technical Field
[0001] This application relates to the technical field of plant extraction and purification, and in particular to a highly efficient and environmentally friendly method for high-purity extraction of Boletus julibrissin alkaloids. Background Technology
[0002] As a traditional medicinal plant, *Gynostemma pentaphyllum* has a long history of medicinal use in my country. Its roots, stems, and leaves are rich in various bioactive alkaloids, such as sanguisorbin and celandine. These components exhibit a variety of pharmacological activities, including antibacterial, anti-inflammatory, antitumor, and insecticidal effects, making *Gynostemma pentaphyllum* potentially valuable in pharmaceuticals, veterinary medicine, and pesticides. Early extraction methods for *Gynostemma pentaphyllum* were relatively crude and simple, often employing traditional solvent extraction methods. For example, organic solvents such as ethanol and methanol were used to directly soak the raw material, followed by alkaline precipitation, followed by alcohol and acid extraction to extract the alkaloids and other active ingredients. However, this method has many drawbacks. On the one hand, the extraction efficiency is relatively low, often requiring long extraction times and large amounts of solvent, leading to increased production costs. On the other hand, the extraction selectivity is poor, resulting in high impurity content in the extracted material. Subsequent separation and purification steps are cumbersome and complex, causing loss of active ingredients, reducing product purity and quality, and generating large amounts of industrial wastewater. Summary of the Invention
[0003] To improve the purity of the alkaloids obtained, this application provides an efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids.
[0004] This application provides a highly efficient and environmentally friendly method for extracting high-purity alkaloids from *Botrytis cinerea*, employing the following technical solution: A highly efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids includes the following steps: Crushing: The dried fruit pods of the Bo Luo Hui fruit were crushed to obtain the extracted clean material; Grinding: Mix the extracted clean material with acid water, and then grind it through a colloid mill to obtain a grinding liquid; Homogenization: The grinding slurry was added to a nano homogenizer, heated and homogenized for extraction, and then filtered to obtain filtrate A; Purification: Filtrate A is introduced into a chromatographic column for impurity removal, purification, and eluent preparation; wherein the packing material in the chromatographic column includes macroporous resin, and the eluent in the chromatographic column includes glycolic acid, sulfuric acid, and solvent; Concentration: The eluent is concentrated to obtain a concentrated solution; Cooling crystallization: The concentrate is cooled and crystallized to obtain a mixture; Vacuum drying: The mixture is filtered to obtain filtrate B and filter cake. The filter cake is then vacuum dried to obtain product A. Neutralization: Neutralize and filter filtrate B, concentrate and filter the resulting filtrate C to obtain filtrate D; Spray drying: The filtrate D is spray dried to obtain product B.
[0005] By adopting the above technical solution, alkaloids can be more effectively separated from plant tissues using nano-extraction, thereby improving the extraction rate. Macroporous adsorption resin is selected for adsorption, followed by chromatographic purification. The target product is eluted in one step with a composite eluent composed of glycolic acid and sulfuric acid, avoiding multiple elutions, reducing solvent usage, and minimizing losses. This results in a simple process, high extraction rate, high product quality, and high purity.
[0006] In one specific implementation, during the pulverization step, the mesh size of the extracted clean material is 10-30 mesh.
[0007] In one specific implementation, during the grinding step, the acidic solution includes one of sulfuric acid solution, hydrochloric acid solution, and nitric acid solution, and the volume fraction of acid in the acidic solution is 0.2-0.6%; the mass-to-volume ratio of the extracted purified material to the acidic solution is 400g:4L.
[0008] By adopting the above technical solution, using sulfuric acid solution, hydrochloric acid solution, and nitric acid solution as acid water, the purified material can be extracted effectively. Among them, sulfuric acid solution not only has a high extraction rate but also has lower equipment requirements.
[0009] In one specific implementation, the homogenization step involves heating at a temperature of 80–90°C for 1–2 hours.
[0010] By adopting the above technical solution, the heating temperature and time during homogenization are further limited, thereby enabling alkaloids to be extracted more effectively.
[0011] In one specific implementation, the purification step involves first rinsing with hot pure water for 1 BV until the wash solution is clear, then eluting with an eluent for 4 BV, followed by regeneration with rinsing with 40°C hot pure water for 3 BV, until the wash solution is clear, colorless, and odorless. The flow rates for column loading, rinsing, and elution are controlled at 2–3 BV to obtain the eluent. The macroporous resin includes one of D101 and LX-60. The eluent contains 80–90% glycolic acid and 0.2–0.4% sulfuric acid by volume.
[0012] In one specific implementation, in the concentration step, the eluent is concentrated to 10-20% of its volume to obtain a concentrated solution.
[0013] In one specific implementation, the cooling crystallization step involves a crystallization temperature of -5 to -3°C and a time of 0.5 to 1 hour.
[0014] In one specific implementation scheme, the vacuum drying step is performed at a temperature of 65-80°C for 1-2 hours.
[0015] In one specific implementation, the neutralization step is as follows: adding a neutralizing agent to filtrate B for neutralization, filtering, concentrating the resulting filtrate C to 50% volume, filtering, and obtaining filtrate D; wherein the neutralizing agent includes one of calcium carbonate, calcium bicarbonate, and calcium hydroxide.
[0016] In one specific implementation, during the spray drying step, the filtrate D is kept at 40-50°C.
[0017] In summary, this application includes at least one of the following beneficial technical effects: The method in this application utilizes nano-extraction to more effectively separate alkaloids from plant tissues, thereby increasing the extraction rate. Macroporous adsorption resin is selected for adsorption, followed by chromatographic purification. The target product is eluted in one step with a composite eluent composed of glycolic acid and sulfuric acid, avoiding multiple elutions, reducing solvent usage, and minimizing losses. This results in a simple process with a high extraction rate, high product quality, and high purity. The method in this application uses only one alcohol solvent, which is easy to manage safely and reduces pollution. The method in this application can obtain high-purity sanguisorbin and chelidonine alkaloid product A, and low-purity protopine and allopicrin alkaloid product B. Attached Figure Description
[0018] Figure 1 This is the liquid chromatogram used in Example 1 to represent product A.
[0019] Figure 2 This is the liquid chromatogram used in Example 1 to represent product B.
[0020] Figure 3 This is the liquid chromatogram used in Example 2 to represent Product A.
[0021] Figure 4 This is the liquid chromatogram used in Example 2 to represent product B.
[0022] Figure 5 This is the liquid chromatogram used in Example 3 to represent Product A.
[0023] Figure 6 This is the liquid chromatogram used in Example 3 to represent product B.
[0024] Figure 7 This is the liquid chromatogram used in Example 4 to represent Product A.
[0025] Figure 8This is the liquid chromatogram used in Example 4 to represent product B.
[0026] Figure 9 This is the liquid chromatogram used in Example 5 to represent Product A.
[0027] Figure 10 This is the liquid chromatogram used in Example 5 to represent product B.
[0028] Figure 11 This is the liquid chromatogram used in Example 6 to represent Product A.
[0029] Figure 12 This is the liquid chromatogram used in Example 6 to represent product B.
[0030] Figure 13 It is a standard chromatogram of liquid chromatography. Detailed Implementation
[0031] The present application will be further described in detail below with reference to the embodiments.
[0032] All raw materials used in the examples were commercially available. The dried fruit pods of *Bletilla striata* were supplied by a supplier in Chaling County, Zhuzhou City, Hunan Province. Example
[0033] Example 1 Example 1 provides a highly efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids, comprising the following steps: Crushing: The dried fruit pods of the Bo Luo Hui fruit were crushed to obtain a 30-mesh purified material; Grinding: Mix 400g of the extracted material with 4L of acidic water, then grind it to 2000 mesh using a colloid mill to obtain a grinding solution; the acidic water is a sulfuric acid solution, and the volume fraction of hydrochloric acid in the sulfuric acid solution is 0.4%; Homogenization: Add the grinding liquid to a nano homogenizer, heat to 90℃ and homogenize for 1 hour, filter to obtain filtrate A; Purification: Filtrate A was fed into the chromatographic column at a flow rate of 20 ml / min. After the feed was complete, the column was first washed with hot pure water for 1 BV until the wash solution was clear. Then, 4 BV of eluent was eluted with the eluent at a flow rate of 25 ml / min. The column was then regenerated by washing with 3 BV of hot pure water at 40°C until the wash solution was clear, colorless, and odorless. The eluent was obtained by using D101 macroporous resin as the packing material in the chromatographic column. The eluent consisted of a mixture of glycolic acid, sulfuric acid, and a solvent, with glycolic acid comprising 90% by volume, sulfuric acid comprising 0.4% by volume, and methanol as the solvent. Concentration: Concentrate the eluent to 15% by volume to obtain the concentrate; Cooling crystallization: The concentrated solution was cooled and crystallized at -5℃ for 1 hour to obtain a mixture; Vacuum drying: The mixture was filtered to obtain filtrate B and filter cake. The filter cake was vacuum dried at 75°C for 1.5 h to obtain product A. The liquid chromatogram of product A is shown in the figure below. Figure 1 ; Neutralization: Add a neutralizing agent to filtrate B to neutralize it to neutral, filter, concentrate the resulting filtrate C to 50% of its volume, filter, and obtain filtrate D; wherein the neutralizing agent is calcium carbonate; Spray drying: Filtrate D was kept at 45°C and then spray dried to obtain product B. The liquid chromatogram of product B is shown in the figure below. Figure 2 .
[0034] Example 2 Example 2 provides a highly efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids, comprising the following steps: Crushing: The dried fruit pods of the Bo Luo Hui fruit were crushed to obtain a 30-mesh purified material; Grinding: Mix 400g of the extracted material with 4L of acidic water, then grind it to 2000 mesh using a colloid mill to obtain a grinding solution; the acidic water is a sulfuric acid solution, and the volume fraction of hydrochloric acid in the sulfuric acid solution is 0.4%; Homogenization: Add the grinding liquid to a nano homogenizer, heat to 80°C and homogenize for 1 hour, then filter to obtain filtrate A; Purification: Filtrate A was fed into the chromatographic column at a flow rate of 20 ml / min. After the feed was complete, the column was first washed with hot pure water for 1 BV until the wash solution was clear. Then, 4 BV of eluent was eluted with the eluent at a flow rate of 25 ml / min. The column was then regenerated by washing with 3 BV of hot pure water at 40°C until the wash solution was clear, colorless, and odorless. The eluent was obtained by using D101 macroporous resin as the packing material in the chromatographic column. The eluent consisted of a mixture of glycolic acid, sulfuric acid, and a solvent, with glycolic acid comprising 90% by volume, sulfuric acid comprising 0.4% by volume, and methanol as the solvent. Concentration: Concentrate the eluent to 15% by volume to obtain the concentrate; Cooling crystallization: The concentrated solution was cooled and crystallized at -5℃ for 1 hour to obtain a mixture; Vacuum drying: The mixture was filtered to obtain filtrate B and filter cake. The filter cake was vacuum dried at 75°C for 1.5 h to obtain product A. The liquid chromatogram of product A is shown in the figure below. Figure 3 ; Neutralization: Add a neutralizing agent to filtrate B to neutralize it to neutral, filter, concentrate the resulting filtrate C to 50% of its volume, filter, and obtain filtrate D; wherein the neutralizing agent is calcium carbonate; Spray drying: Filtrate D was kept at 45°C and then spray dried to obtain product B. The liquid chromatogram of product B is shown in the figure below. Figure 4 .
[0035] Example 3 Example 3 provides a highly efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids, comprising the following steps: Crushing: The dried fruit pods of the Bo Luo Hui fruit were crushed to obtain a 30-mesh purified material; Grinding: Mix 400g of the extracted material with 4L of acidic water, then grind it to 2000 mesh using a colloid mill to obtain a grinding solution; the acidic water is a sulfuric acid solution, and the volume fraction of hydrochloric acid in the sulfuric acid solution is 0.6%; Homogenization: Add the grinding liquid to a nano homogenizer, heat to 90℃ and homogenize for 1 hour, filter to obtain filtrate A; Purification: Filtrate A was fed into the chromatographic column at a flow rate of 20 ml / min. After the feed was complete, the column was first washed with hot pure water for 1 BV until the wash solution was clear. Then, 4 BV of eluent was eluted with the eluent at a flow rate of 25 ml / min. The column was then regenerated by washing with 3 BV of hot pure water at 40°C until the wash solution was clear, colorless, and odorless. The eluent was obtained by using D101 macroporous resin as the packing material in the chromatographic column. The eluent consisted of a mixture of glycolic acid, sulfuric acid, and a solvent, with glycolic acid comprising 90% by volume, sulfuric acid comprising 0.4% by volume, and methanol as the solvent. Concentration: Concentrate the eluent to 15% by volume to obtain the concentrate; Cooling crystallization: The concentrated solution was cooled and crystallized at -5℃ for 1 hour to obtain a mixture; Vacuum drying: The mixture was filtered to obtain filtrate B and filter cake. The filter cake was vacuum dried at 75°C for 1.5 h to obtain product A. The liquid chromatogram of product A is shown in the figure below. Figure 5 ; Neutralization: Add a neutralizing agent to filtrate B to neutralize it to neutral, filter, concentrate the resulting filtrate C to 50% of its volume, filter, and obtain filtrate D; wherein the neutralizing agent is calcium carbonate; Spray drying: Filtrate D was kept at 45°C and then spray dried to obtain product B. The liquid chromatogram of product B is shown in the figure below. Figure 6 .
[0036] Example 4 Example 4 provides a highly efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids, comprising the following steps: Crushing: The dried fruit pods of the Bo Luo Hui fruit were crushed to obtain a 30-mesh purified material; Grinding: Mix 400g of the extracted material with 4L of acidic water, then grind it to 2000 mesh using a colloid mill to obtain a grinding solution; the acidic water is a sulfuric acid solution, and the volume fraction of hydrochloric acid in the sulfuric acid solution is 0.2%; Homogenization: Add the grinding liquid to a nano homogenizer, heat to 90℃ and homogenize for 1 hour, filter to obtain filtrate A; Purification: Filtrate A was fed into the chromatographic column at a flow rate of 20 ml / min. After the feed was complete, the column was first washed with hot pure water for 1 BV until the wash solution was clear. Then, 4 BV of eluent was eluted with the eluent at a flow rate of 25 ml / min. The column was then regenerated by washing with 3 BV of hot pure water at 40°C until the wash solution was clear, colorless, and odorless. The eluent was obtained by using D101 macroporous resin as the packing material in the chromatographic column. The eluent consisted of a mixture of glycolic acid, sulfuric acid, and a solvent, with glycolic acid comprising 90% by volume, sulfuric acid comprising 0.4% by volume, and methanol as the solvent. Concentration: Concentrate the eluent to 15% by volume to obtain the concentrate; Cooling crystallization: The concentrated solution was cooled and crystallized at -5℃ for 1 hour to obtain a mixture; Vacuum drying: The mixture was filtered to obtain filtrate B and filter cake. The filter cake was vacuum dried at 75°C for 1.5 h to obtain product A. The liquid chromatogram of product A is shown in the figure below. Figure 7 ; Neutralization: Add a neutralizing agent to filtrate B to neutralize it to neutral, filter, concentrate the resulting filtrate C to 50% of its volume, filter, and obtain filtrate D; wherein the neutralizing agent is calcium carbonate; Spray drying: Filtrate D was kept at 45°C and then spray dried to obtain product B. The liquid chromatogram of product B is shown in the figure below. Figure 8 .
[0037] Example 5 Example 5 provides a highly efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids, comprising the following steps: Crushing: The dried fruit pods of the Bo Luo Hui fruit were crushed to obtain a 30-mesh purified material; Grinding: Mix 400g of the extracted material with 4L of acidic water, then grind it to 2000 mesh using a colloid mill to obtain a grinding solution; the acidic water is a sulfuric acid solution, and the volume fraction of hydrochloric acid in the sulfuric acid solution is 0.4%; Homogenization: Add the grinding liquid to a nano homogenizer, heat to 90℃ and homogenize for 1 hour, filter to obtain filtrate A; Purification: Filtrate A was fed into the chromatographic column at a flow rate of 20 ml / min. After the feed was complete, the column was first washed with hot pure water for 1 BV until the wash solution was clear. Then, 4 BV of eluent was eluted with the eluent at a flow rate of 25 ml / min. The column was then regenerated by washing with 40°C hot pure water for 3 BV until the wash solution was clear, colorless, and odorless. The eluent was obtained by using LX-60 macroporous resin as the packing material and a mixture of glycolic acid, sulfuric acid, and solvent as the eluent. The volume fraction of glycolic acid in the eluent was 90%, the volume fraction of sulfuric acid was 0.4%, and the solvent was methanol. Concentration: Concentrate the eluent to 15% by volume to obtain the concentrate; Cooling crystallization: The concentrated solution was cooled and crystallized at -5℃ for 1 hour to obtain a mixture; Vacuum drying: The mixture was filtered to obtain filtrate B and filter cake. The filter cake was vacuum dried at 75°C for 1.5 h to obtain product A. The liquid chromatogram of product A is shown in the figure below. Figure 9 ; Neutralization: Add a neutralizing agent to filtrate B to neutralize it to neutral, filter, concentrate the resulting filtrate C to 50% of its volume, filter, and obtain filtrate D; wherein the neutralizing agent is calcium carbonate; Spray drying: Filtrate D was kept at 45°C and then spray dried to obtain product B. The liquid chromatogram of product B is shown in the figure below. Figure 10 .
[0038] Example 6 Example 6 provides a highly efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids, comprising the following steps: Crushing: The dried fruit pods of the Bo Luo Hui fruit were crushed to obtain a 30-mesh purified material; Grinding: Mix 400g of the extracted material with 4L of acidic water, then grind it to 2000 mesh using a colloid mill to obtain a grinding solution; the acidic water is a nitric acid solution, and the volume fraction of nitric acid in the nitric acid solution is 0.4%; Homogenization: Add the grinding liquid to a nano homogenizer, heat to 90℃ and homogenize for 1 hour, filter to obtain filtrate A; Purification: Filtrate A was fed into the chromatographic column at a flow rate of 20 ml / min. After the feed was complete, the column was first washed with hot pure water for 1 BV until the wash solution was clear. Then, 4 BV of eluent was eluted with the eluent at a flow rate of 25 ml / min. The column was then regenerated by washing with 3 BV of hot pure water at 40°C until the wash solution was clear, colorless, and odorless. The eluent was obtained by using D101 macroporous resin as the packing material in the chromatographic column. The eluent consisted of a mixture of glycolic acid, sulfuric acid, and a solvent, with glycolic acid comprising 90% by volume, sulfuric acid comprising 0.4% by volume, and methanol as the solvent. Concentration: Concentrate the eluent to 15% by volume to obtain the concentrate; Cooling crystallization: The concentrated solution was cooled and crystallized at -5℃ for 1 hour to obtain a mixture; Vacuum drying: The mixture was filtered to obtain filtrate B and filter cake. The filter cake was vacuum dried at 75°C for 1.5 h to obtain product A. The liquid chromatogram of product A is shown in the figure below. Figure 11 ; Neutralization: Add a neutralizing agent to filtrate B to neutralize it to neutral, filter, concentrate the resulting filtrate C to 50% of its volume, filter, and obtain filtrate D; wherein the neutralizing agent is calcium carbonate; Spray drying: Filtrate D was kept at 45°C and then spray dried to obtain product B. The liquid chromatogram of product B is shown in the figure below. Figure 12 .
[0039] Comparative Example 1 Comparative Example 1 provides a method for preparing high-content berberine alkaloids, comprising the following steps: Weigh 300 kg of *Botrytis cinerea* pods, crush them to 90 mesh, and put them into the material metering tank of the atomization extraction equipment. Pump dilute sulfuric acid with a pH of 2-3 into the solvent tank of the equipment. Start the automatic atomization extraction process. After about 2.5 hours, the pre-filtered extract is obtained. After centrifugation and filtration through an 800-mesh filter cloth, the extract is adsorbed onto an AB-8 macroporous resin column at a rate of 2-3 BV / hour, controlling the total alkaloid content to resin content to be 10:1-15:1. First, wash with purified water at a rate of 3-4 BV / hour until the effluent is colorless. Then, elute with 85-90% ethanol at a rate of 2 BV / hour until the effluent is colorless. Collect the ethanol eluent. Concentrate the eluent under reduced pressure and dry it to obtain approximately 4.6 kg of total alkaloids from *Botrytis cinerea*.
[0040] Product testing: The quality of the products and the content of alkaloids in each example and comparative example were tested.
[0041] Table 1. Performance test results of the products
[0042] Combining Example 1 and Comparative Example 1, the product yield in Example 1 is higher, and the alkaloid content is also higher. It can be seen that by using the extraction method in this application, alkaloids can be separated from plant tissues more effectively using nano-extraction, thereby improving the extraction rate. Macroporous adsorption resin is used for adsorption, followed by chromatographic purification. The target product is eluted in one step with a composite eluent composed of glycolic acid, sulfuric acid, and solvent, avoiding multiple elutions, reducing solvent usage, and reducing losses. This simplifies the process steps and improves the product yield and purity.
[0043] Combining Examples 1 and 2, the yield in Example 1 is higher, indicating that increasing the temperature during the heating and homogenization extraction of the grinding slurry will increase the product yield.
[0044] Combining Examples 1, 3, and 4, it can be seen that the concentration of sulfuric acid solution in Example 1 is optimal during grinding. When the concentration of sulfuric acid solution is low, the yield and purity of the product decrease accordingly, while when the concentration of sulfuric acid solution is high, the effect is negligible.
[0045] Combining Examples 1 and 5, it can be seen that when the packing material in the chromatographic column is replaced with LX-60 macroporous resin during column purification, both the yield and purity of the product decrease.
[0046] Combining Examples 1 and 6, it can be seen that using nitric acid solution as acid water during grinding reduces both the yield and purity of the product.
[0047] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.
Claims
1. A highly efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids, characterized in that: Includes the following steps: Crushing: The dried fruit pods of the Bo Luo Hui fruit were crushed to obtain the extracted clean material; Grinding: The extracted material is mixed with acidic water and then ground through a colloid mill to obtain a grinding solution; the acidic water includes one of sulfuric acid solution, hydrochloric acid solution, and nitric acid solution, and the volume fraction of acid in the acidic water is 0.2-0.6%; the mass-volume ratio of the extracted material to the acidic water is 400g:4L; Homogenization: The grinding slurry was added to a nano homogenizer, heated for homogenization and extraction, and filtered to obtain filtrate A; wherein the heating temperature was 80-90℃ and the time was 1-2h; Purification: Filtrate A is introduced into a chromatographic column for impurity removal, purification, and eluent preparation; wherein the packing material in the chromatographic column includes macroporous resin, and the eluent in the chromatographic column includes glycolic acid, sulfuric acid, and solvent; Concentration: The eluent is concentrated to obtain a concentrated solution; Cooling crystallization: The concentrate is cooled and crystallized to obtain a mixture; Vacuum drying: The mixture is filtered to obtain filtrate B and filter cake. The filter cake is then vacuum dried to obtain product A. Neutralization: Neutralize and filter filtrate B, concentrate and filter the resulting filtrate C to obtain filtrate D; Spray drying: The filtrate D is spray dried to obtain product B.
2. The efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids according to claim 1, characterized in that: In the pulverization step, the mesh size of the extracted clean material is 10-30 mesh.
3. The efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids according to claim 1, characterized in that: In the purification step, first rinse 1 BV with hot pure water until the washing solution is clear, then elute 4 BV with eluent, and then regenerate 3 BV with 40°C hot pure water until the washing solution is clear, colorless, and odorless. The flow rate for column loading, rinsing, and elution is controlled at 2-3 BV to obtain the eluent. The macroporous resin includes one of D101 and LX-60. The eluent contains 80-90% glycolic acid and 0.2-0.4% sulfuric acid by volume.
4. The efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids according to claim 1, characterized in that: In the concentration step, the eluent is concentrated to 10-20% of its volume to obtain a concentrated solution.
5. The efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids according to claim 1, characterized in that: In the cooling crystallization step, the crystallization temperature is -5 to -3℃ and the time is 0.5 to 1 hour.
6. The efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids according to claim 1, characterized in that: In the vacuum drying step, the drying temperature is 65-80℃ and the time is 1-2 hours.
7. The efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids according to claim 1, characterized in that: The neutralization step is as follows: add a neutralizing agent to filtrate B for neutralization, filter, concentrate the obtained filtrate C to 50% volume, filter, and obtain filtrate D; The neutralizing agent includes one of calcium carbonate, calcium bicarbonate, and calcium hydroxide.
8. The efficient and environmentally friendly method for high-purity extraction of *Botrytis cinerea* alkaloids according to claim 1, characterized in that: In the spray drying step, the filtrate D is kept at 40-50°C during spray drying.