Cytochrome p450 enzyme activity detection probe, method and application
By designing detection probes containing P450 enzyme-specific substrates and reporter molecule precursors, and combining click chemistry and bioluminescence detection, the problem of accurate quantitative assessment of cytochrome P450 enzyme activity in the human body has been solved, realizing a highly sensitive, modular, and high-throughput detection method suitable for new drug development and clinical applications.
CN122146289APending Publication Date: 2026-06-05ZUNYI MEDICAL UNIVERSITY
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- ZUNYI MEDICAL UNIVERSITY
- Filing Date
- 2026-02-11
- Publication Date
- 2026-06-05
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Figure CN122146289A_ABST
Abstract
The present application relates to the field of biotechnology and analytical chemistry, and discloses a cytochrome P450 enzyme activity detection probe, which comprises a P450 enzyme specific substrate and a reporter precursor; the structure of the P450 enzyme specific substrate comprises a part that can be recognized by a specific P450 enzyme subtype, a part that can be catalytically converted by the P450 enzyme subtype, and a first coupling functional group; the structure of the reporter precursor comprises a luciferin structural unit that can be recognized by luciferase and a second coupling functional group; the second coupling functional group is complementary to the first coupling functional group and is covalently connected through a click chemistry reaction, and the product of the click chemistry reaction is a stable luciferase substrate. The probe of the present application can safely and efficiently accurately quantify the cytochrome P450 enzyme activity in the human body.
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