Paracoccus sp. and industrial use thereof

Abstract

The application discloses a paracoccus and industrial application thereof. The paracoccus is classified as paracoccus sp. Paracoccus sp. , which has been preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC32753 and a preservation date of November 22, 2024, and a preservation address of No. 3, Xili, Beichen West Road, Chaoyang District, Beijing. The strain can efficiently remove ammonia nitrogen and COD in wastewater, exhibits high deamination performance and good storage stability, has a wide pH and temperature application range, and can effectively remove organic pollutants in water bodies when combined with other strains or bacterial agents such as bacillus, and has a good industrial application prospect.

Description

Technical Field

[0001] This application relates to a strain of paracoccus and its industrial applications, and belongs to the field of biotechnology. Background Technology

[0002] Ammonia nitrogen is one of the binding indicators for the total discharge of major pollutants into my country's water environment. As a nutrient in water bodies, ammonia nitrogen can lead to eutrophication and poison aquatic life. Furthermore, incomplete conversion of ammonia nitrogen produces nitrite; if consumed over a long period, nitrite in the water combines with proteins to form nitrosamines, which are extremely harmful to human health. Ammonia nitrogen in the environment mainly comes from domestic sewage, industrial wastewater, agricultural wastewater, and centralized pollution treatment facilities. According to the "2023 China Ecological Environment Statistical Yearbook," the national ammonia nitrogen discharge in 2023 was 1.193 million tons. Compared to other wastewater, industrial wastewater has a wider range of sources, contains more pollutants, and includes toxic substances, posing a significant threat to the environment and human health.

[0003] Currently, the main methods for treating ammonia nitrogen wastewater include physical, chemical, and biological methods. Generally, physical and chemical methods are more expensive, and some methods are prone to secondary pollution. Therefore, green and inexpensive biological methods are increasingly being used by enterprises. Currently, the biological treatment of ammonia nitrogen-containing wastewater mainly relies on traditional biological denitrification processes centered on autotrophic nitrifying bacteria. Autotrophic nitrifying bacteria have long generation cycles and are extremely sensitive to environmental fluctuations. Under conditions of high ammonia nitrogen loads, high concentrations of other pollutants, and heavy metals in complex industrial wastewater, their activity is easily inhibited, making it difficult for them to perform their degradation function. The characteristics of industrial wastewater quality limit the application of autotrophic nitrifying bacteria in industrial wastewater treatment. Heterotrophic strains that can adapt to complex water qualities and efficiently remove ammonia may be the key to treating ammonia nitrogen in industrial wastewater.

[0004] Paracocci are a group of Gram-negative bacteria widely distributed in the environment. Due to their metabolic diversity, they have shown promising applications in the field of biological denitrification. These bacteria can typically utilize multiple carbon sources and possess good environmental adaptability, making them a highly promising microbial resource for treating aquaculture wastewater, landfill leachate, and soil remediation.

[0005] Although the denitrification function of *Paracococcus* has been preliminarily reported, these reports still have shortcomings in practical applications. For example, there is still considerable room for improvement in the stability of deamination gene expression, deamination efficiency, and rate. Therefore, it is particularly necessary to develop a strain that can stably express deamination genes and still possess highly efficient deamination performance in industrial wastewater. Summary of the Invention

[0006] The purpose of this invention is to address the shortcomings of existing biological methods for ammonia nitrogen removal. This application identifies a highly efficient ammonia-removing strain, PLH095, from Paracoccus. The strain's ammonia removal performance and stability were tested, demonstrating high efficiency, good storage stability, and a wide applicable pH and temperature range, indicating promising prospects for industrial application.

[0007] To achieve the above objectives, this application adopts the following technical solution: Firstly, this application provides a strain of *Paragonimus*, which is classified and named *Paragonimus*. Paracoccussp It has been deposited at the China General Microbiological Culture Collection Center, with accession number CGMCC32753, deposit date November 22, 2024, and deposit address No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing. Secondly, this application provides the application of Paracoccus as described in the first aspect in water treatment.

[0008] In some embodiments, the water treatment includes removing at least one of ammonia nitrogen, COD, and organic pollutants from wastewater or sewage; ammonia nitrogen refers to free ammonia (NH3) or ammonium ions (NH4) in the water. ＋ The total number of nitrogen compounds existing in the form of ) In some embodiments, the pH of the water treatment is 6-9, preferably 7-9, and more preferably 8; In some embodiments, the water treatment temperature is 25~40°C, preferably 25~40°C, and more preferably 35°C.

[0009] In some embodiments, the organic pollutants include volatile phenolic compounds and / or quinoline compounds.

[0010] Thirdly, this application provides a biological agent comprising the Paracoccus strain as described in the first aspect or comprising a bacterial agent prepared from the Paracoccus strain as described in the first aspect.

[0011] In some embodiments, the preparation method of the biological agent includes: inoculating the paracoccus as described in the first aspect into ADM medium for fermentation culture, collecting the fermentation broth after the culture is completed, centrifuging to remove the supernatant to obtain the bacterial cells, freeze-drying the bacterial cells to prepare lyophilized powder, and adding or not adding excipients, other strains or bacterial agents to obtain the product.

[0012] In some embodiments, the fermentation culture process conditions are: 33~37℃, pH: 7.0±0.5; DO: 60%-80%.

[0013] In some embodiments, the excipients are selected from at least one of water-soluble adjuvant powder, adsorbent (activated carbon), pH adjuster (calcium carbonate, sodium hydroxide, hydrochloric acid), and defoamer (polydimethylsiloxane, polyoxyethylene glycerol ether). In some embodiments, the other bacterial species or agents include Bacillus species or agents prepared from Bacillus.

[0014] Fourthly, this application provides the use of the biological agents described in the third aspect in water treatment.

[0015] In some embodiments, the water treatment includes removing at least one of ammonia nitrogen, COD, and organic pollutants from sewage or wastewater; In some embodiments, the pH of the water treatment is 6-9, preferably 7-9, and more preferably 8; In some embodiments, the water treatment temperature is 25~40°C, preferably 25~40°C, and more preferably 35°C.

[0016] In some embodiments, the organic pollutants include volatile phenolic compounds and / or quinoline compounds.

[0017] Compared with the prior art, this application has the following beneficial effects: 1) Highly efficient ammonia removal performance This strain exhibits highly efficient deamination capabilities, with advantages including rapid growth and high biomass. The OD of the bacterial culture after 24 hours in ADM medium was [data missing]. 600 It can reach 3.5; the bacteria have vigorous metabolic activity and a fast adsorption and conversion rate of ammonia-containing substrates in the environment; and it has good adaptability to the environment, which enables it to start up quickly and proliferate rapidly in biological denitrification of wastewater, thereby shortening the treatment cycle.

[0018] 2) Stable expression of deamination factor Using freshly activated PLH095 as the control group, and PLH095 stored at 4℃ for about 30 days, 60 days, and 90 days as the experimental group, a heterotrophic ammonia removal experiment was conducted. The results showed that the refrigerated PLH095 still maintained high activity, and its removal effect on ammonia nitrogen and COD was not significantly different from that of the control group.

[0019] 3) It can be formulated into biological agents (bacterial liquid, bacterial powder, compound bacterial agent, etc.) for application in different types of industrial wastewater. The bacterial agent, formulated with PLH095 as the core bacterium and combined with other types of functional bacteria, can not only effectively degrade ammonia nitrogen in industrial wastewater, but also promote the degradation of organic matter in wastewater and improve the stability of the system. Attached Figure Description

[0020] Figure 1Image of Paracoccus PLH095 cultured on an agar plate.

[0021] Figure 2 Microscopic image of Paracoccus PLH095.

[0022] Figure 3 Example photograph of a single-strain inoculum of Paracoccus PLH095.

[0023] Figure 4 Example photograph of a compound microbial agent with Paracoccus PLH095 as the main component.

[0024] Preservation Instructions Paracoccus PLH095 is classified and named Paracoccus. Paracoccussp It was deposited on November 22, 2024, at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC32753. Detailed Implementation

[0025] To make the technical solution of this application clearer and easier to understand, preferred embodiments are described in detail below with reference to the accompanying drawings.

[0026] This application provides a strain with stable deammoniation performance and its application effect in actual wastewater treatment, offering effective assistance in solving the problems of poor strain stability and low denitrification efficiency in current biological deammoniation technologies for water treatment. Using activated sludge from a petrochemical plant in Binzhou City, Shandong Province as a sample, after enrichment culture in LB medium, and using heterotrophic nitrification medium as the screening medium, *Paracoccus* PLH095 (preserved at the China General Microbiological Culture Collection Center, accession number CGMCC32753) was screened. Using ADM medium as the test medium, heterotrophic deammoniation performance tests were conducted to verify the deammoniation performance of PLH095 and the stability of its deamination factor expression. This *Paracoccus* exhibits rapid growth, strong adaptability, stable deammoniation performance, and a wide pH range, making it a promising candidate for industrial application. Biological agents prepared with PLH095 as the core functional bacteria are applied to the deammoniation treatment of various industrial wastewaters. They can not only achieve rapid deammoniation in actual wastewater, but also have a good removal effect on total nitrogen in wastewater. In addition, they also have a good removal effect on toxic substances in wastewater such as volatile phenols and quinolines.

[0027] The following description, in conjunction with specific embodiments, illustrates that the experimental or testing methods described in the embodiments are conventional methods unless otherwise specified; the reagents and materials described are obtained from conventional commercial channels unless otherwise specified. Among them, the *Priscilla megaterium* and *Bacillus subtilis* used in the embodiments are commercially available strains purchased from Hubei Qiming Biotechnology Co., Ltd.

[0028] Example 1: Screening, purification and identification of Paracoccus 1.1 Sample Collection The sample was obtained from activated sludge from a petrochemical plant in Binzhou City, Shandong Province.

[0029] 1.2 Strain screening Take 1 mL of the above activated sludge and inoculate it into 100 mL / 250 mL LB liquid medium for enrichment culture. Culture at 30 ℃ and 200 rpm on a shaker for 48 h to obtain the enriched solution. (The composition of LB liquid medium is: 10.0 g / L tryptone, 5.0 g / L yeast extract, 10.0 g / L sodium chloride, pH: 7.0±0.1) Take 1 mL of the above enrichment solution and inoculate it into 100 mL / 250 mL heterotrophic nitrification medium for enrichment culture. Incubate at 30 ℃ and 200 rpm on a shaker for 48 h. Repeat this operation 2-3 times. (Heterotrophic nitrification medium: NH4Cl 0.71 g / L, KH2PO4 0.5 g / L, K2HPO4 0.5 g / L, CH3COONa 2.8 g / L, magnesium sulfate heptahydrate 1 g / L, pH: 7.0±0.1) Take 1 mL of the above heterotrophic nitrification medium and serially dilute it, then spread it onto LB solid medium and incubate at 30°C. (LB solid medium is liquid medium with 15 g / L agar powder added.) 1.3 Strain purification After colonies have grown on LB agar plates, colonies with different morphologies are selected for streak culture. After 24 hours of culture, single colonies are picked and streaked three more times to obtain purified strains.

[0030] from Figure 1 It can be seen that the colonies formed by PLH095 after being cultured at 35℃ for 24 hours on LB medium are flesh-pink, round, and have a smooth and moist surface.

[0031] from Figure 2 As can be seen, PLH095 appears as a short rod under a microscope.

[0032] 1.4 Strain Identification The purified strain was sent for 16S rRNA sequencing. The obtained sequence was compared with the database of the National Center for Biotechnology Information (NCBI) in the United States. Combined with the morphological and physiological biochemical analysis results, strain PLH095 was identified as Paracoccus. This strain was deposited on November 22, 2024, at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with the accession number CGMCC32753.

[0033] The sequence of the 16S rRNA is as follows (SEQ ID NO: 1): Example 2: Deammoniation performance of Paracoccus PLH095 and preparation of single-strain agent 2.1 Deamination performance test of the strain A slant culture stored at 4 ℃ was used. A loopful was scraped from the culture and inoculated into sterilized test medium (ADM medium: NH4Cl 0.71 g / L, yeast extract 1 g / L, glucose 1 g / L, KH2PO4 0.5 g / L, CH3COONa 2.8 g / L, magnesium sulfate heptahydrate 1 g / L, pH: 7.0±0.1) in a biosafety cabinet. The culture was incubated at 30 ℃ and 200 rpm for 48 h using a constant temperature shaker. The deamination performance of PLH095 was then tested. Ammonia nitrogen, COD, and OD were measured. 600 。 (OD) 600 For the tests, the mixed culture medium was diluted a certain factor and detected by spectrophotometer at a wavelength of 600 nm. For the tests of ammonia nitrogen and COD, the culture medium was filtered through a 0.22 μm filter membrane, and the filtrate was diluted a certain factor before testing. Ammonia nitrogen was determined using the Nessler's reagent spectrophotometric method (HJ 535-2009), and COD was determined using the potassium dichromate method (HJ 828-2017). The test results are shown in Table 1. Table 1

[0034] Table 1 shows that Paracoccus PLH095 grows relatively quickly, with an OD value of [missing value] at 24 h. 600 The ammonia nitrogen concentration reached 3.5; it can rapidly remove ammonia nitrogen, removing approximately 200 mg / L of ammonia nitrogen in 24 hours (70.44%), 92.93% in 36 hours, and 94.7% in 48 hours. Furthermore, this paracoccus also showed good removal efficiency for COD in water, achieving a COD removal rate of 89.91% in 48 hours.

[0035] 2.2 Stability test of strains Freshly activated PLH095 was used as the control group (Group A), and PLH095 slant cultured at 4℃ for approximately 60, 180, and 360 days were used as experimental groups (Groups B, C, and D). All samples were inoculated with test medium (ADM, formula as in 2.1) and cultured at 30℃ and 200 rpm for 48 hours. Ammonia nitrogen, COD, and OD were measured at 24h, 36h, and 48h. 600 The test results are shown in Table 2: Table 2

[0036] Table 2 shows that the ammonia nitrogen removal rate, COD removal rate, and OD removal rate of experimental groups B and C compared with the control group are different. 600There were no significant differences; the removal rates of ammonia nitrogen remained above 90%, and the removal rates of COD were above 85%. The bacterial growth trend and activity were close to those of the control group. Group D was stored for approximately one year, and still maintained about half the ammonia nitrogen and COD removal rates of the control group. This indicates that the PLH095 strain can maintain a certain level of deammoniation performance and metabolic stability during long-term storage, possessing the potential for long-term storage and repeated use. This characteristic significantly reduces the strain maintenance cost and provides a stable and reliable technical guarantee for practical engineering applications.

[0037] 2.3 Preparation method of single-strain inoculum PLH095 was fermented in a 5L fermenter using ADM medium with 0.1-0.5% antifoaming agent. The fermentation temperature was controlled at 35℃, pH 7.0±0.5, and DO (Dissolved Oxygen Relative Saturation) at 60%-80%. Once the fermentation entered the logarithmic phase, feed was slowly introduced using 500mL of 10x concentrated ADM solution. Fermentation was stopped after 20-30 hours. At the end of fermentation, the OD of the fermentation broth was measured. 600 The concentration remained stable above 20. The bacterial culture was concentrated by centrifugation using a benchtop high-speed refrigerated centrifuge (model MH1850R, Shanghai Merrick), and then freeze-dried using a freeze dryer (model: CoolSafe Pro 55-9, manufacturer: LaboGene, Denmark) to prepare a freeze-dried powder. The TVC (Total Viable Count) reached 3 × 10⁻⁶. 10 CFU / g. Prepared to 5.0 × 10⁻⁶ using water-soluble excipients (a common composite carrier composed of various inorganic salts and special excipients, manufacturer: Zaozhuang Youshun). 9 Single-strain inoculum of CFU / g.

[0038] Example 3: Effect of pH on the deammoniation performance of PLH095 single-strain inoculum In real-world aquatic environments, significant pH fluctuations can affect the metabolic activity of bacterial strains. To investigate the deammoniation performance of PLH095 under different pH conditions, ADM medium was used as the test medium, with initial pH values ​​of 5.0, 6.0, 7.0, 8.0, and 9.0 for each experimental group. The PLH095 single-strain agent from section 2.3 was inoculated at a concentration of 0.1‰ (m:v(g / mL)), and cultured at 30 ℃ and 200 rpm for 36 h. Ammonia nitrogen and OD were then measured. 600 The test results are shown in Table 3: Table 3

[0039] Table 3 shows that the ammonia nitrogen removal rate was highest at pH 8.0, reaching 96.37%, and the OD... 600The value was 4.65; the removal rate was lowest at pH 5.0, at 35.12%, and the OD... 600 The value was 2.1. Under pH conditions of 7.0 and 9.0, the ammonia nitrogen removal rates were 91.47% and 85.65%, respectively, and the OD... 600 The values ​​of 4.19 and 3.98 indicate that the strain maintains high metabolic activity in neutral to slightly alkaline environments. Although slightly acidic conditions (pH 6.0) slightly inhibit the performance of PLH095, the removal rate still remains above 65%, indicating that the bacterium has good environmental tolerance. Experimental results show that the PLH095 single-strain agent has a strong pH adaptability range, maintaining stable deammoniation efficiency within the pH range of 6.0 to 9.0, and is suitable for functioning in slightly alkaline environments. The pH should be controlled between 7.0 and 9.0, with 8.0 being the optimal value.

[0040] Example 4: Effect of temperature on the deammoniation performance of PLH095 single-strain inoculum To investigate the deammoniation performance of PLH095 at different temperatures, ADM medium was used as the test medium, and the culture temperatures for each experimental group were set at 20.0, 25.0, 30.0, 35.0, and 40.0℃. The PLH095 single-strain agent from section 2.3 was inoculated at a concentration of 0.1‰ (m:v(g / mL)), with a pH of 8.0±0.5, and cultured at 200 rpm for 36 h. Ammonia nitrogen and OD were then measured. 600 The test results are shown in Table 4: Table 4

[0041] Table 4 shows that the ammonia nitrogen removal rate was highest at 35.0℃, reaching 98.31%, and the OD... 600 The value was 4.85; the lowest removal rate was 63.99% at 20.0℃, and the OD... 600 Only 2.19. At 30.0℃ and 40.0℃, the removal rates were 96.36% and 94.50%, respectively, with cell growth remaining at a high level. The results indicate that PLH095 possesses good ammonia removal ability within the temperature range of 25.0–40.0℃, with the optimal temperature being 35.0℃.

[0042] Example 5: Application Test of Compound Bacterial Agent on Actual Wastewater 1 A compound bacterial agent with *Paracoccus paracoccus* PLH095 as the main component was prepared. *Priscilla megaterium* and *Bacillus subtilis* were used, and LB medium was used as the fermentation medium, achieving a total TVC of 5*10⁻⁶. 10 Single-strain inoculum preparations were made using the method described in section 2.3 with a CFU / g or higher, and the TVC of the single-strain inoculum was 5.0 × 10⁻⁶. 9 CFU / g. The three single-strain inoculants were thoroughly mixed to prepare a total colony count of 5.0 x 10⁻⁶. 9The compound microbial agent has a CFU / g concentration, in which the single microbial agent of Paracoccus PLH095 accounts for more than 35%, and the single microbial agents of Priestella megaterium and Bacillus subtilis are added at 10~50%.

[0043] Wastewater from an aerobic tank in a petrochemical plant was used as the test sample (COD 760 mg / L, ammonia nitrogen 49.92 mg / L, total nitrogen 58 mg / L, organic nitrogen 8.08 mg / L). The pH of the wastewater was adjusted to 8.0. 1 L of wastewater was placed in a 2 L beaker, and the inoculum size of PLH095 compound bacterial agent was 0.1‰ (m:v(g / mL)). The control group received no bacterial agent. An aerobic reaction was conducted at room temperature using an aerator and a stirrer, maintaining the pH at 7.5 ± 0.5 throughout the reaction. After 36 h of incubation, the concentrations of ammonia nitrogen and total nitrogen were measured. (Total nitrogen was determined using the alkaline potassium persulfate digestion ultraviolet spectrophotometric method HJ 636—2012). The test results are shown in Table 5. Table 5

[0044] As shown in Table 5, the PLH095 compound microbial agent achieved a 96.33% removal rate of ammonia nitrogen, a 93.90% removal rate of total nitrogen, and a 81.75% removal rate of COD after 36 hours of reaction.

[0045] Example 6: Application Test of Compound Bacterial Agent in Actual Wastewater 2 The same compound bacterial agent as in Example 5 was used. Wastewater from the same flotation tank as in Example 5 was used as the experimental water sample (COD 1690 mg / L, ammonia nitrogen 57.12 mg / L, total nitrogen 86 mg / L, organic nitrogen 28.88 mg / L). The pH of the wastewater was adjusted to 8.0. The denitrification performance of the bacterial agent was tested using the above wastewater. After 36 hours of cultivation, relevant indicators were measured using the same testing method as in Example 5. The test results are shown in Table 6. Table 6

[0046] Table 6 shows that after 36 hours of reaction, the PLH095 compound bacterial agent reduced ammonia nitrogen to 2.89 mg / L, with a removal rate of 94.94%; and total nitrogen to 9.53 mg / L, with a removal rate of 88.91%. In contrast, the removal rates of ammonia nitrogen and total nitrogen in the control group were 8.82% and 6.81%, respectively. This bacterial agent not only demonstrated strong denitrification ability in actual wastewater but also showed good COD removal effect.

[0047] Example 7: Application Test of Compound Bacterial Agent to Actual Wastewater 3 The same compound bacterial agent as in Example 5 was used. A sample of ammonia stripping wastewater from a coking plant was used. After testing various indicators such as ammonia nitrogen and pH, the sample was treated as follows: diluted twice, and the pH was adjusted to 8.0. The treated sample was used as the experimental sample (COD 2948 mg / L, ammonia nitrogen 102 mg / L, volatile phenols 268.33 mg / L, quinoline 100.06 mg / L, total phosphorus 0.98 mg / L). The denitrification performance of the bacterial agent was tested using the above wastewater. Due to the high biotoxicity of this wastewater, it was cultured for 48 hours before testing. The testing method was the same as in Example 5. (Volatile phenols were determined using the 4-aminoantipyrine spectrophotometric method HJ 503-2009; quinoline was determined using liquid chromatography-mass spectrometry). The test results are shown in Table 7. Table 7

[0048] As shown in Table 7, the PLH095 compound bacterial agent achieved a 95.23% removal rate of ammonia nitrogen and an 80.79% removal rate of COD in the ammonia-containing wastewater after 48 hours. This bacterial agent not only demonstrated strong denitrification capabilities but also maintained good removal efficiency for toxic substances in the system, with a 96.73% removal rate of volatile phenols and a 92.04% removal rate of quinoline.

[0049] The above description is merely a preferred embodiment of this application and is not intended to limit this application in any form or substance. It should be noted that those skilled in the art can make several improvements and additions without departing from this application, and these improvements and additions should also be considered within the scope of protection of this application.

Claims

1. A strain of paracoccus, characterized in that, The paracoccus is classified and named paracoccus. Paracoccussp It has been deposited at the China General Microbiological Culture Collection Center, with accession number CGMCC32753, deposit date November 22, 2024, and deposit address No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing.

2. The application of Paracoccus as described in claim 1 in water treatment.

3. The application according to claim 2, characterized in that, The water treatment includes removing at least one of ammonia nitrogen, COD, and organic pollutants from sewage or wastewater; And / or, the pH of the water treatment is 6-9; And / or, the water treatment temperature is 25~40℃.

4. The application according to claim 3, characterized in that, The organic pollutants include volatile phenolic compounds and / or quinoline compounds.

5. A biological agent, characterized in that, The biological agent comprises the Paracoccus strain of claim 1 or a bacterial agent prepared from the Paracoccus strain of claim 1.

6. The biological agent according to claim 5, characterized in that, The preparation method of the biological agent includes: inoculating the Paracoccus of claim 1 into ADM medium for fermentation culture, collecting the fermentation broth after the culture is completed, centrifuging to remove the supernatant to obtain the bacterial cells, freeze-drying the bacterial cells to make freeze-dried powder, and adding or not adding excipients, other strains or bacterial agents to obtain the product.

7. The biological agent according to claim 6, characterized in that, The fermentation culture process conditions are: 33~37℃, pH: 7.0±0.5; DO: 60%-80%; And / or, the excipients are selected from at least one of the following: carrier, adsorbent, cosolvent, dispersant, stabilizer, antioxidant, pH adjuster, preservative, defoamer, slow-release agent and binder; And / or, the other bacterial species or agents include Bacillus species or agents prepared from Bacillus.

8. The use of the biological agent according to any one of claims 5 to 7 in water treatment.

9. The application according to claim 8, characterized in that, The water treatment includes removing at least one of ammonia nitrogen, COD, and organic pollutants from sewage or wastewater; And / or, the pH of the water treatment is 6-9; And / or, the water treatment temperature is 25~40℃.

10. The application according to claim 9, characterized in that, The organic pollutants include volatile phenolic compounds and / or quinoline compounds.

Images