Bioengineered bile ducts based on organoids and gas-liquid interfaces and methods of construction thereof

Abstract

The application discloses a method for constructing bioengineered bile duct based on bile duct organoids and gas-liquid interface culture, which amplifies bile duct epithelial cells through bile duct organoid culture technology, and amplifies fibroblasts derived from bile duct through adherent culture; then, the two kinds of cells are mixed with collagen to form a compound at a specific ratio, inoculated on a porous support, and sequentially subjected to submerged culture and gas-liquid interface culture to form an engineered bile duct cell sheet layer with a double-layer structure; finally, the sheet layer is wound on a mold for maturation culture, so that a tubular engineered bile duct is constructed. The application solves the problems of in-vitro amplification difficulty and function maintenance of bile duct epithelial cells by using organoid technology, and successfully constructs a bioengineered bile duct which is complete in structure, good in barrier function and strong in mechanical toughness by using a gas-liquid interface co-culture method, thereby providing a new way for model research, drug screening and clinical transplantation of biliary diseases.

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