A human hair follicle organoid culture solution, culture system, culture method and application

Abstract

The present application provides a kind of human hair follicle organoid culture solution, culture system and culture method, belong to the field of biotechnology.The present application provides a kind of human hair follicle stem cell as seed cell and is induced to be constructed into the culture solution of hair follicle organoid, with DMEM / F12 and Neurobasal as base culture solution, and a variety of additives and key small molecules are combined.The present application also mixes the culture solution with primary keratinocyte culture solution, obtains a kind of microenvironment culture system supporting human hair follicle organoid development, the system culture cycle is short, and typical globular protrusion structure organoid is induced in about 6 days of culture.Reproduced human hair follicle organoid, with natural hair follicle is highly similar, and reproduces key microenvironment component, exhibits highly consistent molecular expression profile and potential physiological function with natural hair follicle.

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