Acidic aflatoxin oxidase qh50, encoding gene and application thereof

Abstract

The application belongs to the field of agricultural biotechnology, and particularly relates to an acid aflatoxin oxidase QH50, a coding gene thereof and application. The acid aflatoxin oxidase QH50 has an optimal reaction pH of 6.0 for oxidizing and degrading AFB1, and has a relative enzyme activity of 83.1% at pH 4.0 and a relative enzyme activity of 64.4% at pH 3.0. After incubation at pH 3.0 for 3 h, the residual enzyme activity is maintained at 69.2% of the initial enzyme activity, and after incubation at pH 4.0-8.0 for 3 h, the residual enzyme activity is maintained at more than 80%, which can well adapt to the weak acidic environment of the intestinal tract of livestock and poultry and has good acid-base stability. The QH50 protein loaded on a carrier as a biological material can efficiently degrade AFB1 in corn steep liquor without adjusting the pH, and can also be applied to degrade aflatoxins in feed and feed raw materials, corn steep liquor or Chinese herbal medicines and their extracts, and has good application prospect.

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