DRG9基因在调控水稻耐高温性中的应用

By cloning and editing the rice DRG9 gene, and using CRISPR technology to construct DRG9 mutants or overexpress the DRG9 gene, the problem of insufficient heat resistance in rice has been solved, and high-efficiency growth and high seed setting rate of rice under high-temperature conditions have been achieved.

CN122146778BActive Publication Date: 2026-07-17HUAZHONG AGRI UNIV +2

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Filing Date
2026-05-09
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

There is a lack of effective means in the current technology to improve the heat resistance of rice, especially to breed heat-resistant rice varieties through genetic engineering.

Method used

The rice DRG9 gene was cloned and edited using CRISPR technology. DRG9 mutants or overexpressions of the DRG9 gene were constructed using gene editing technology to regulate the heat resistance of rice. DRG9 gene expression was regulated by methods such as DNA methylation, histone deacetylation, and RNA interference.

Benefits of technology

It significantly improves the heat resistance of rice, enhances its growth performance under high temperature conditions, and increases the seed setting rate.

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Abstract

本发明属于植物基因工程技术领域,公开了DRG9基因在调控水稻耐高温性中的应用。本发明通过分离、克隆和通过功能验证得到一种能够提高水稻对高温耐受能力的DRG9基因,所述DRG9基因编码的蛋白质序列如SEQ ID.2所示。本发明克隆到控制水稻高温应答基因DRG9,并对候选基因进行CRISPR突变体表型鉴定,通过苗期和成株期高温胁迫表型鉴定表明,缺失该基因片段时,水稻耐高温胁迫能力降低,超量表达时,耐高温能力提高,证实了该基因的功能及应用途径。
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