A brain metastasis cell line based on flow cytometry sorting and transcarotid directional injection and a construction method and application thereof

Abstract

The application discloses a brain metastasis cell line based on flow cytometry sorting and carotid artery directional injection and a construction method and application thereof, relates to the technical field of tumor biology and animal model construction, and is characterized in that: taking mouse lung cancer LLC cells as starting cells, luciferase genes and green fluorescent protein genes are introduced into the starting cells through a lentivirus system, high-purity GFP positive cells are screened through flow cytometry, and starting tumor cells stably expressing reporter genes are obtained; the tumor cells are preferentially delivered to brain tissues through blood vessel ligation and then directional injection of the carotid artery; after brain metastases are formed, tumor cells are separated from the brain tissues, target cells are screened again through flow cytometry, and in-vitro expansion culture is carried out; the obtained tumor cells are used for in-vivo screening again, and the above-mentioned screening is repeated, so that a brain metastasis cell line which can be stably passed on and has a relatively high brain metastasis ability is finally obtained, and the brain metastasis cell line provides a reliable experimental tool for lung cancer brain metastasis mechanism research and drug screening.

Images