Preparation method of polypeptide of arnebia euchroma, polypeptide of arnebia euchroma and application thereof

Abstract

The application belongs to the technical field of biotechnology, and particularly relates to a preparation method of a polypeptide of Arnebia euchroma, the polypeptide of Arnebia euchroma and application thereof. The preparation method comprises the following steps: extracting Arnebia euchroma after CO2 supercritical extraction, adding water to obtain an extract; performing enzyme hydrolysis in cooperation with Bacillus subtilis, neutral protease and amino peptidase to obtain an enzyme hydrolysate; performing preliminary filtration on the enzyme hydrolysate, and sequentially separating the obtained filtrate through an ultrafiltration membrane and a nanofiltration membrane to collect a nanofiltration concentrated solution; and drying to obtain the polypeptide of Arnebia euchroma. The polypeptide of Arnebia euchroma prepared by the method has high polypeptide content, strong anti-inflammatory, anti-aging, anti-oxidation activities, and can be applied to functional cosmetics or medicines, and is helpful to the development and utilization of Arnebia euchroma resources.

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