Primer and probe combination and detection method for real-time fluorescent PCR detection of bacterial canker of peach

By designing a combination of primers and probes for real-time fluorescent PCR detection with high specificity and sensitivity, the problems of cumbersome operation and long time consumption in the existing technology have been solved, realizing rapid and accurate detection of bacterial canker pathogens in peach trees, which is suitable for import and export plant quarantine and biosafety monitoring.

CN122146906APending Publication Date: 2026-06-05HUANGPU CUSTOMS TECH CENT

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUANGPU CUSTOMS TECH CENT
Filing Date
2026-04-23
Publication Date
2026-06-05

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Abstract

The application discloses a peach bacterial canker bacterium real-time fluorescent PCR detection primer and probe combination and a detection method, and belongs to the technical field of biological detection. The peach bacterial canker bacterium real-time fluorescent PCR detection method disclosed by the application uses a to-be-detected bacterial strain DNA as a template, and utilizes a primer and a probe combination to perform real-time fluorescent PCR detection. The detection method of the application has high specificity, high sensitivity, is rapid, efficient, simple to operate, and objective in result interpretation, and provides technical support for detection and monitoring of the peach bacterial canker bacterium in imported seedlings.
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