一种DNA条形码序列及其应用
By designing specific primers F and R, PCR amplification and sequencing were performed based on the conserved region of the aceE gene, and a phylogenetic tree was constructed. This solved the problem of insufficient resolution in the classification and identification of Clavibacter michiganensis in the existing technology, and achieved high-specificity identification and accurate differentiation of the tomato canker pathogen.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- CHINA AGRI UNIV
- Filing Date
- 2026-05-08
- Publication Date
- 2026-07-17
AI Technical Summary
Existing DNA barcoding technology has insufficient resolution in the classification and identification of Clavibacter michiganensis, and cannot effectively distinguish closely related species. Universal primers have low amplification efficiency and contain repetitive units or regions that are difficult to sequence, which cannot meet the needs of accurate identification of the tomato canker pathogen.
Specific primers F and R were designed based on the conserved regions of the aceE gene in various species within the Clavibacter genus. The nucleotide sequence is SEQ ID NO.1. The amplification length was controlled at 400-700 bp, and the melting temperature was less than 69℃. The primers were used for PCR amplification and sequencing, and a phylogenetic tree was constructed for identification.
It achieves highly specific identification of Clavibacter michiganensis, accurately distinguishing it from other species within the genus and demonstrating strong species-level resolution and accuracy.
Smart Images
Figure CN122146909B_ABST