SNP marker for identifying flower bud stage of lonicera japonica and application thereof

Through multi-omics analysis of honeysuckle germplasm resources, SNP markers related to the budding stage were discovered and dCAPS markers were developed, which solved the problem of unclear genetic basis of long budding stage in honeysuckle, realized efficient budding stage identification and variety breeding, and improved the medicinal value and economic benefits of honeysuckle.

CN122146913APending Publication Date: 2026-06-05INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
Filing Date
2024-12-05
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

The genetic basis of the long bud stage of honeysuckle is unclear in the existing technology, which makes it difficult to achieve efficient variety breeding and quality improvement, thus affecting its medicinal value and economic benefits.

Method used

By conducting multi-omics joint analysis of honeysuckle germplasm resources, SNP marker sites significantly associated with the budding stage were discovered, and a dCAPS marker method was developed. By combining specific primer PCR with restriction enzyme digestion, a detection method and primers for the budding stage of honeysuckle were developed, enabling accurate identification of the budding stage.

Benefits of technology

This study provides an efficient molecular marker-assisted selection method that can accurately identify the flowering stage of Lonicera japonica without evaluating phenotypic characteristics, supporting the molecular identification and breeding of Lonicera japonica medicinal materials and improving harvest yield and quality.

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Abstract

The application belongs to the technical field of biology, and specifically discloses a SNP marker for identifying Lonicera japonica flower bud stage and application thereof. The application finds that the SNP site Chr7:24636061 is related to the Lonicera japonica flower bud stage through multi-omics joint analysis of Lonicera japonica germplasm resources, and further develops a dCAPS marker for the site, and provides a method for detecting the Lonicera japonica flower bud stage and special primers (SEQ ID NO. 2-3). Experiments prove that the primers provided by the application can specifically detect the genotype of the Chr7:24636061 site in Lonicera japonica, and can also be used as a new tool for marker-assisted selection in breeding of different flower bud stages, and the flower bud stage of the material carrying the AA genotype is significantly higher than that of the material carrying the GG or AG genotype.
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