一种与茎瘤田菁不定根原基性状相关的SNP标记及检测引物对和应用
By developing SNP molecular markers and designing specific primer pairs on chromosome 4 of sesquiterpenoid scab, the problem of locating and identifying adventitious root primordia of sesquiterpenoid scab was solved, enabling rapid breeding and efficient screening, which has significant breeding application value.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
- Filing Date
- 2026-05-11
- Publication Date
- 2026-07-17
AI Technical Summary
The lack of effective SNP molecular markers in existing technologies for the localization and identification of adventitious root primordia in stem nodules of Sesbania sesquiterpenes leads to high breeding costs, long cycles, and difficulty in achieving multi-gene aggregation and genetic gain.
A SNP molecular marker located at 10,433,968 bp on chromosome 4 of the reference genome of *Senecio scandens* was developed, and specific primer pairs (SEQ ID NO.1 and SEQ ID NO.2) were designed for PCR amplification and sequencing to identify the presence or absence of adventitious root primordia in *Senecio scandens*.
This method enables rapid identification of adventitious root primordia in stem-nodled sesbania, shortens the breeding cycle, improves screening efficiency, and has significant potential for breeding applications.
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Figure CN122146934B_ABST