Construction of a library of transcription factors from sugarcane smut fungus and its application in screening of effector protein regulatory factors

CN122147544APending Publication Date: 2026-06-05GUANGXI UNIV

Patent Information

Authority / Receiving Office: CN · China
Patent Type: Applications(China)
Current Assignee / Owner: GUANGXI UNIV
Filing Date: 2026-01-15
Publication Date: 2026-06-05

AI Technical Summary

Technical Problem

Existing technologies have gaps in the integration of transcription factor resources and high-throughput screening of effector protein regulatory factors from *Ustilago maydis*, resulting in low screening efficiency, high costs, and insufficient research coverage.

Method used

A transcription factor library of *Ustilago maydis* was constructed. The transcription factor sequence information of *Ustilago maydis* was obtained, and the transcription factors were ligated into the vector pGADT7 using PCR amplification and recombinase to construct recombinant plasmids. The plasmids were then expanded into *E. coli* culture, and positive interacting transcription factors were obtained through two rounds of screening using yeast one-hybrid screening.

Benefits of technology

High-throughput screening of transcription factors of sugarcane smut fungus was achieved, the regulatory mechanism of pathogenic transcription factors was elucidated, and efficient technical support for control targets of sugarcane smut was provided.

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Abstract

The application discloses a construction method of a sugarcane smut fungus transcription factor library, and first, according to the identified corn smut fungus transcription factor sequence information, the full-length sequences of all the transcription factors of the sugarcane smut fungus are obtained through the complete genome data and the annotation information of the sugarcane smut fungus; then, the transcription factors are connected to the vector pGADT7 through PCR amplification and recombination enzymes, the recombination plasmid with the transcription factors is obtained, and the construction of the transcription factor library is completed. Accordingly, the inventor constructs a library containing 268 transcription factors of the sugarcane smut fungus, and uses the transcription factor library to screen the transcription factor SsbHLH-1 which regulates the expression of the effector protein SsPele1 of the sugarcane smut fungus. The application provides the feasibility for high-throughput screening of the transcription factors of the sugarcane smut fungus, and provides efficient technical support for analyzing the regulation mechanism of the pathogenic related transcription factors of the sugarcane smut fungus and excavating the target points for the prevention and treatment of smut.

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