一种无需薄切片的植物细根解剖结构测定装置及方法

The device and method for determining the anatomical structure of plant fine roots without the need for thin sections, through direct staining and stable positioning microscopic observation, solves the problems of cumbersome operation and error introduction in existing technologies, and realizes rapid and reliable determination of anatomical structures, which is suitable for multi-sample and batch analysis.

CN122149961BActive Publication Date: 2026-07-17NORTHEAST NORMAL UNIVERSITY

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHEAST NORMAL UNIVERSITY
Filing Date
2026-05-07
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing technologies for studying the anatomical structure of plant fine roots are cumbersome, time-consuming, and rely on specialized equipment and techniques. Furthermore, sample processing is prone to introducing errors, making it difficult to meet the needs of rapid determination and high-throughput analysis.

Method used

This invention provides a device and method for determining the anatomical structure of fine plant roots without the need for thin sections. Through direct staining, stable positioning, and microscopic observation, using a glass slide, sample container, limiting metal wire, and light source, it achieves clear observation of the cross-section of fine roots and determination of anatomical parameters, eliminating the need for dehydration, embedding, and ultrathin sectioning steps.

Benefits of technology

It simplifies the sample preparation process, improves measurement efficiency, reduces costs, ensures the integrity of anatomical structures and the reliability of measurements, and is suitable for multi-sample and batch analysis.

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Abstract

本发明涉及植物科学技术领域,尤其涉及一种无需薄切片的植物细根解剖结构测定装置及方法。通过对细根横截面直接染色、稳定定位与显微观察,在不进行脱水、包埋及超薄切片处理的情况下,即可获得清晰、完整的细根横截面解剖图像,并实现对解剖参数的可靠测定。省去了固定、脱水、包埋及超薄切片等步骤,显著简化了样品制备流程,缩短了从处理到成像的时间,提高了整体测定效率,适用于多样本及批量分析场景。在保证解剖结构可识别和测量可靠的前提下,实现了流程简化、效率提升与成本降低,具有良好的技术价值与应用前景。
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