A fresh dog food to enhance immunity

Abstract

This invention provides a dog food that enhances immunity, belonging to the field of functional pet food technology. The dog food that enhances immunity is composed of component A and Bacillus subtilis. By weight percentage, component A includes the following ingredients: beef 39%–41%, beef cartilage 9%–11%, beef liver 9%–11%, cooked egg 9%–11%, purple sweet potato 9%–11%, carrot 4%–6%, broccoli 4%–6%, lettuce 4%–6%, cranberry 4%–6%, fish oil 0.2%, achyranthes bidentata 0.1%, shark chondroitin 0.02%, whey calcium 1%, malt powder 0.8%, inulin 0.5%, and inactivated brewer's yeast 0.5%. The live Bacillus subtilis count in the dog food is 1*10⁻⁶. 9 CFU / g. The dog food provided by this invention, by regulating the types and proportions of raw materials, enables the active ingredients to work synergistically, and can significantly enhance the antiviral ability of puppies after 14 days of feeding.

Description

Technical Field

[0001] This invention relates to the field of functional pet food technology, and in particular to a fresh dog food that enhances immunity. Background Technology

[0002] Traditional dog food has some technological limitations in enhancing immunity. Most dog food on the market today uses high-temperature processing, which can effectively kill bacteria and extend shelf life, but it also causes immune-enhancing ingredients (such as chondroitin) to decompose due to high temperatures.

[0003] Furthermore, while traditional dog food may contain some immune-boosting ingredients, it lacks the synergistic effect with the active ingredients in foods like animal liver and purple sweet potatoes. Animal liver is rich in nutrients such as vitamins A and D, while purple sweet potatoes are rich in anthocyanins and dietary fiber, all of which play a positive role in enhancing immunity. However, in the processing of traditional dog food, these ingredients are not properly mixed and processed, failing to fully exert their synergistic effect. For example, vitamins A and D are easily oxidized and deactivated during high-temperature processing, and anthocyanins are also easily degraded during processing.

[0004] The limitations of this processing technology prevent traditional dog food from fully utilizing the synergistic effects of various nutrients, resulting in limited antibody enhancement. Therefore, traditional dog food has significant shortcomings in boosting immunity, necessitating further improvements in its processing technology and formulation to enhance the activity and synergistic effects of immune-enhancing components. To address this, this invention provides a fresh dog food that enhances immunity, thereby resolving the aforementioned problems. Summary of the Invention

[0005] In view of this, the purpose of this invention is to provide a fresh dog food that enhances immunity, in order to solve the problem that the immune-enhancing ingredients (such as chondroitin) in traditional dog food are easily decomposed due to high-temperature processing and lack synergy with the active ingredients in animal liver and purple sweet potato, resulting in limited antibody enhancement effects.

[0006] To achieve the above objectives, the present invention provides the following technical solution: One of the technical solutions of this invention is a fresh dog food that enhances immunity, the raw materials of which consist of component A and Bacillus subtilis; By weight percentage, component A comprises the following ingredients: beef 39%–41%, beef cartilage 9%–11%, beef liver 9%–11%, cooked egg 6%–8%, purple sweet potato 9%–11%, carrot 4%–6%, broccoli 4%–6%, lettuce 4%–6%, cranberry 4%–6%, fish oil 0.2%, achyranthes bidentata 0.1%, shark cartilage extract 0.02%, whey calcium 1%, malt powder 0.8%, inulin 0.5%, and inactivated brewer's yeast 0.5%. The live Bacillus subtilis count in the dog food was 1*10^6. 9CFU / g.

[0007] In a preferred embodiment of the present invention, component A comprises the following ingredients by mass percentage: 40% beef, 10% beef cartilage, 10% beef liver, 6.88% cooked egg, 10% purple sweet potato, 5% carrot, 5% broccoli, 5% lettuce, 5% cranberry, 0.2% fish oil, 0.1% achyranthes bidentata, 0.02% shark cartilage extract, 1% whey calcium, 0.8% malt powder, 0.5% inulin, and 0.5% inactivated brewer's yeast; The live Bacillus subtilis count in the dog food was 1*10^6. 9 CFU / g.

[0008] The immune-boosting fresh dog food provided by this invention is suitable for puppies aged 0-1 years. Feeding puppies for 14 days can significantly enhance their immunity and significantly improve their antiviral (canine distemper virus, canine parvovirus) levels.

[0009] The second technical solution of the present invention is a method for preparing the above-mentioned immune-enhancing fresh dog food, comprising the following steps: Step 1, Cleaning: Clean the beef, beef cartilage, beef liver, purple sweet potato, carrot, broccoli and lettuce; Step 2, Steaming: Steam the beef, beef cartilage, beef liver, carrots, purple sweet potatoes, broccoli, and lettuce. Step 3: Crush the ingredients from Step 2 separately, crush the hard-boiled eggs, and then mix the crushed ingredients with cranberries, fish oil, achyranthes bidentata, shark chondroitin, whey calcium, malt powder, inulin, inactivated brewer's yeast and Bacillus subtilis evenly. Step 4: Filling; Step 5: Vacuum packaging; Step 6: Sterilize.

[0010] In a preferred embodiment of the present invention, in step 2, when the beef, beef cartilage, beef liver, carrots, purple sweet potatoes, broccoli, and lettuce are steamed, the different materials are steamed at different temperatures and for different times: beef and beef cartilage are steamed at 80°C for 2 hours, beef liver is steamed at 80°C for 1.5 hours, carrots and purple sweet potatoes are steamed at 80°C for 40 minutes, and broccoli and lettuce are steamed at 80°C for 15 minutes.

[0011] In a preferred embodiment of the present invention, the cooked egg is prepared by boiling a raw egg at 100°C for 7 minutes and then removing the shell.

[0012] In a preferred embodiment of the present invention, the time for stirring and mixing to achieve uniformity is 5 minutes.

[0013] In a preferred embodiment of the present invention, the sterilization method is pasteurization. After sterilization, the metabolites of Bacillus subtilis continue to exert their effects, synergistically enhancing the immunity of puppies with other active ingredients.

[0014] In a preferred embodiment of the present invention, the pasteurization specifically involves sterilization at 75°C for 20 minutes, followed by freezing; the freezing temperature is less than or equal to -22°C.

[0015] The present invention discloses the following technical effects: The dog food provided by this invention, by regulating the types and proportions of raw materials, enables the active ingredients to work synergistically, and can significantly enhance the antiviral ability of puppies after 14 days of feeding.

[0016] The preparation method provided by this invention avoids problems such as oxidation inactivation and degradation of active ingredients during processing through reasonable treatment, thereby making the antibody enhancement effect of puppies after they eat fresh dog food obvious. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 The percentage change in weight of puppies fed continuously for 14 days in Example 1; Figure 2 The level of viral antibodies in the blood of puppies fed continuously for 14 days in Example 1. Detailed Implementation

[0019] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0020] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0021] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0022] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0023] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0024] Unless otherwise specified, the technical solutions described in this invention are all conventional solutions in the field, and the reagents or raw materials used are all purchased from commercial channels or are publicly available unless otherwise specified.

[0025] To better understand the present invention, the following embodiments further illustrate the content of the present invention, but the content of the present invention is not limited to the following embodiments.

[0026] Example 1 A fresh dog food to boost immunity, made from ingredients consisting of component A and Bacillus subtilis; By weight percentage, component A comprises the following ingredients: 40% beef, 10% beef cartilage, 10% beef liver, 6.88% cooked egg, 10% purple sweet potato, 5% carrot, 5% broccoli, 5% lettuce, 5% cranberry, 0.2% fish oil, 0.1% achyranthes bidentata, 0.02% shark cartilage extract, 1% whey calcium, 0.8% malt powder, 0.5% inulin, and 0.5% inactivated brewer's yeast; The live count of Bacillus subtilis in the fresh dog food was 1*10⁻⁶. 9 CFU / g.

[0027] The preparation method for the above-mentioned immune-boosting fresh dog food includes the following steps: Step 1, Cleaning: After thawing the beef, beef cartilage and beef liver, clean them for 3 minutes. Clean the purple sweet potato, carrot, broccoli and lettuce and cut them into pieces. Step 2, Steaming: Steam the beef, beef cartilage, beef liver, carrots, purple sweet potatoes, broccoli, and lettuce. The beef and beef cartilage are steamed at 80℃ for 2 hours, the beef liver at 80℃ for 1.5 hours, the carrots and purple sweet potatoes at 80℃ for 40 minutes, and the broccoli and lettuce at 80℃ for 15 minutes. Boil the raw eggs at 100℃ for 7 minutes, then peel them to get hard-boiled eggs. Step 3: Crush the ingredients from Step 2 separately (crush until the particles are small but still recognizable). Then, mix the crushed ingredients with cranberries, fish oil, achyranthes bidentata, shark chondroitin, whey calcium, malt powder, inulin, inactivated brewer's yeast, and Bacillus subtilis for 5 minutes until well combined. Step 4, Filling: Weigh the mixed product in a bowl, ensuring each portion is no less than 100g and no more than 103g. After weighing, pour the mixture into bags through a filling funnel until no food residue remains on the funnel walls. Step 5, Vacuum Packaging: Arrange the filled products neatly on the vacuum packaging machine and vacuum seal them. After sealing, gently press to evenly flatten the product and fill the entire packaging bag; Step 6, Pasteurization: Preheat the water in the pasteurizer to 75 degrees Celsius. Place the packaged products into the pasteurizer for water bath pasteurization. Temperature: 75 degrees Celsius, duration: 20 minutes. After the time is up, immediately remove the products, arrange them neatly, and immediately place them in a flash freezer at -22 degrees Celsius or below. Continue freezing until the products are completely solid.

[0028] The nutritional components of the fresh dog food in this embodiment are shown in Table 1: Table 1

[0029] Effect verification: Six breeds of puppies aged 3-6 months with similar initial weights—Border Collies, Samoyeds, Corgis, Shiba Inus, Poodles, and Huskies—were randomly divided into two groups: Example 1 and Control Group 1, with 3 puppies in each group, for a total of 36 puppies. The experiment lasted 14 days (3 days of pre-experiment and 11 days of formal testing), with feeding twice daily, and the amount of food calculated based on body weight. The Control Group 1 was fed commercially available Myfoodie dry food (purchased from the official flagship store). Blood samples were collected before and after the experiment, and serum levels of canine distemper virus (CDV) and canine parvovirus (CPV) antibodies were detected using ELISA. The improvement rate in body condition was determined based on a 9-point body condition score (BCS), assessed by the same veterinary professional through visual observation (ribs, abdominal contour, lumbar spine palpation) combined with palpation. The BCS (Body Shape Scale) scoring criteria are defined as follows: 1-3 points: Underweight (ribs and lumbar vertebrae are clearly prominent, with very little fat coverage); 4-5 points: Ideal body shape (ribs are palpable but not obviously prominent, waist is prominent, and abdomen is tucked up); 6-9 points: Overweight (ribs are difficult to palpate, fat layer is thick, and abdomen is sagging). A body shape score of 4-5 points at the end of the experiment was considered an improvement. Results showed that the body shape improvement rate in the puppies in Example 1 group reached 100% (Body shape improvement rate (%) = (number of puppies with a body shape score of 4-5 points at the end of the experiment / total number of puppies in the group) × 100%).

[0030] Depend on Figure 1 It can be seen that after feeding the puppies (Border Collies) for 14 consecutive days, the fresh food group (i.e., the fresh dog food in this example) showed a significant increase in weight, with a weight growth rate of 50.3%.

[0031] Weight gain rate = (%) = [(W14 – W0) / W0] × 100% Wherein, W14 is the fasting weight measured on the morning of the 14th day of the experiment, in kg; W0 is the weight measured on an empty stomach on the morning of the day the experiment begins, in kg.

[0032] Depend on Figure 2 It can be seen that after feeding puppies (Border Collies, Bichon Frises, Teddy Bears, etc.) for 14 consecutive days, the antibody levels of the fresh food group (i.e., the fresh dog food in this example) increased significantly, with the serum canine distemper virus antibody level increasing by ≥58.5% and the canine parvovirus antibody level increasing by ≥65.9%.

[0033] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A fresh dog food that enhances immunity, characterized in that, The raw material consists of component A and Bacillus subtilis; By weight percentage, component A comprises the following ingredients: beef 39%–41%, beef cartilage 9%–11%, beef liver 9%–11%, cooked egg 6%–8%, purple sweet potato 9%–11%, carrot 4%–6%, broccoli 4%–6%, lettuce 4%–6%, cranberry 4%–6%, fish oil 0.2%, achyranthes bidentata 0.1%, shark cartilage extract 0.02%, whey calcium 1%, malt powder 0.8%, inulin 0.5%, and inactivated brewer's yeast 0.5%. The live Bacillus subtilis count in the dog food was 1*10^6. 9 CFU / g.

2. A method for preparing the immune-enhancing fresh dog food according to claim 1, characterized in that, Includes the following steps: Step 1, Cleaning: Clean the beef, beef cartilage, beef liver, purple sweet potato, carrot, broccoli and lettuce; Step 2, Steaming: Steam the beef, beef cartilage, beef liver, carrots, purple sweet potatoes, broccoli, and lettuce. Step 3: Crush the ingredients from Step 2 separately, crush the hard-boiled eggs, and then mix the crushed ingredients with cranberries, fish oil, achyranthes bidentata, shark chondroitin, whey calcium, malt powder, inulin, inactivated brewer's yeast and Bacillus subtilis evenly. Step 4: Filling; Step 5: Vacuum packaging; Step 6: Sterilize.

3. The preparation method according to claim 2, characterized in that, In step 2, when the beef, beef cartilage, beef liver, carrots, purple sweet potatoes, broccoli, and lettuce are steamed, the different materials are steamed at different temperatures and for different times: beef and beef cartilage are steamed at 80℃ for 2 hours, beef liver is steamed at 80℃ for 1.5 hours, carrots and purple sweet potatoes are steamed at 80℃ for 40 minutes, and broccoli and lettuce are steamed at 80℃ for 15 minutes.

4. The preparation method according to claim 2, characterized in that, The cooked eggs are prepared by boiling raw eggs at 100°C for 7 minutes, followed by shelling.

5. The preparation method according to claim 2, characterized in that, The time for stirring and mixing to achieve uniformity is 5 minutes.

6. The preparation method according to claim 2, characterized in that, The sterilization method is pasteurization.

7. The preparation method according to claim 6, characterized in that, The pasteurization process specifically involves sterilizing at 75°C for 20 minutes, followed by freezing; the freezing temperature is less than or equal to -22°C.

Images