基因GmISO5在调控大豆籽粒异黄酮组成及抗大豆花叶病毒中的应用

By regulating and editing the GmISO5 gene, the problem of the difficulty in synergistically improving soybean seed quality and disease resistance was solved, and the isoflavone content and antiviral traits of soybean seeds were improved simultaneously, providing a new variety with high nutritional value and high resistance.

CN122168634BActive Publication Date: 2026-07-17INSTITUTE OF CROP SCIENCE CHINESE ACADEMY OF AGRICULTURAL SCIENCES

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INSTITUTE OF CROP SCIENCE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Filing Date
2026-05-12
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In existing technologies, there are limited key gene resources that combine soybean grain quality improvement and soybean mosaic virus resistance traits, making it difficult to achieve synergistic improvement in quality and resistance through single gene manipulation.

Method used

By regulating the GmISO5 gene, the isoflavone content and resistance of soybean seeds can be altered by knocking out or overexpressing this gene. By combining gene editing vectors and sgRNA technology, the GmISO5 gene can be targeted to obtain new soybean varieties with high isoflavone content and high virus resistance.

Benefits of technology

It significantly alters the isoflavone content and composition of soybean seeds, enhances soybean resistance to soybean mosaic virus, and achieves targeted improvement of functional components and simultaneous enhancement of disease resistance in soybean seeds.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明公开基因GmISO5在调控大豆籽粒异黄酮组成及抗大豆花叶病毒中的应用,所述基因GmISO5的核苷酸序列如SEQ ID NO:1所示。本发明通过对大豆群体籽粒异黄酮相关性状进行遗传分析,鉴定获得了与异黄酮含量显著相关的候选基因GmISO5,并通过表达分析及功能验证明确了该基因与大豆籽粒异黄酮性状的密切关联。研究表明,GmISO5能够正向调控大豆籽粒异黄酮的含量及其组成比例。利用基因工程手段对GmISO5进行调控,可显著改变大豆籽粒中异黄酮及其各组分的含量,实现对大豆籽粒功能性成分的定向改良,为培育高异黄酮含量、高营养价值的大豆新品种提供了重要的基因资源和有效的技术手段。
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