A device for measuring the wet weight of bacteria body of a fixed-angle rotor

Abstract

The present application relates to the technical fields of microorganism detection, and especially relates to a bacteria wet weight measuring device for fixed angle rotor, which comprises a tube, the outer side of the tube is provided with a sleeve, the top end of the tube is fixedly connected with an outer edge, the surface of the sleeve is provided with a screw groove, the inner wall of the tube is provided with a filter membrane, the outer side of the tube is provided with a support plate, the support plate and the filter membrane are provided with a flow guide groove, the bottom of the inner wall of the tube is provided with a silica gel strip, and the surface of the tube is provided with a base, when centrifuging the sample with small volume and small capacity and fixing it on the angle rotor, the sample is put into the tube, the tube is put into the sleeve, the tube is clamped on the pipe opening of the sleeve under the action of the outer edge, and the cover is screwed on the surface of the sleeve, because the inner wall of the tube is coated with a polytetrafluoroethylene super-hydrophobic coating, so that the liquid residue on the inner wall can be reduced during use.

Description

Technical Field

[0001] This invention relates to the field of microbial detection technology, and in particular to a device for measuring the wet weight of bacterial cells using a fixed-angle rotor. Background Technology

[0002] In fields such as microbial research, fermentation engineering, and biopharmaceuticals, accurate measurement of cell wet weight is crucial for assessing microbial growth and fermentation efficiency. Cell wet weight measurement reflects microbial biomass by collecting and weighing the cells. Traditional centrifugation methods for measuring cell wet weight often suffer from drawbacks such as cumbersome operation, long processing time, low accuracy (due to residues or sediment loss during supernatant removal after centrifugation, affecting weighing results and reducing accuracy), and susceptibility to human error. Furthermore, traditional filtration and weighing methods involve filtering the bacterial culture sample with filter paper and calculating the cell wet weight by weighing the filter paper before and after filtration. This method requires manual filtration, washing, and weighing, which may result in cell loss, is time-consuming, and is susceptible to environmental factors.

[0003] However, existing methods for measuring bacterial wet weight suffer from common problems such as cumbersome operation, long processing time, low accuracy, and susceptibility to human and environmental interference, failing to meet the demands for rapid and accurate data in modern microbial research and fermentation production. Therefore, developing a simple, rapid, accurate, and highly stable bacterial wet weight measurement technique has become a pressing technical problem in this field. This invention specifically addresses the challenge of measuring low-concentration, small-volume samples in fixed-angle rotor high-speed centrifugation scenarios, complementing the horizontal rotor device.

[0004] In view of this, this paper studies and improves upon existing problems, and provides a bacterial cell wet weight measuring device for a fixed-angle rotor. The aim is to solve the problem and improve its practical value through this technology. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and to propose a bacterial cell wet weight measuring device for a fixed angle rotor.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a bacterial cell wet weight measuring device for a fixed angle rotor, comprising a tube, a sleeve provided on the outer side of the tube, an outer edge fixedly connected to the top end of the tube, a threaded groove provided on the surface of the sleeve, a filter membrane provided on the inner wall of the tube, a support plate provided on the outer side of the tube, a flow guide groove provided between the support plate and the filter membrane, a silicone strip provided at the bottom of the inner wall of the tube, a base provided on the surface of the tube, a polytetrafluoroethylene superhydrophobic coating on the inner wall of the tube, volume scale lines printed on the outer side of the tube, the filter membrane being conical and inclined, and the silicone strip being disposed between the filter membranes.

[0007] Preferably, the top of the sleeve is provided with a cap, the inner wall of the cap is provided with threads, a nitrile gasket is fixedly connected to the inner wall of the cap, a fluororubber gasket is fixedly connected to the bottom of the nitrile gasket, and an annular microgroove is provided between the fluororubber gasket and the inner wall of the cap.

[0008] Preferably, the filter membrane is made of polyethersulfone with a pore size ranging from 0.1 to 0.45 μm, preferably 0.22 μm, and a thickness of 0.05 to 0.2 mm, and is welded to the support plate by laser welding technology.

[0009] Preferably, the tube has multiple flow channels inside, and the multiple flow channels are evenly arranged inside the tube.

[0010] Preferably, the side of the support plate that is in contact with the filter membrane is the front side, and the side of the support plate that is away from the filter membrane is the back side.

[0011] Preferably, a silicone strip is provided at the position where the filter membrane forms a conical angle, the silicone strip completely fills the space of the conical angle, and the upper surface of the silicone strip is higher than the lower end of the filter membrane.

[0012] Compared with the prior art, the beneficial effects of the present invention are:

[0013] 1. When centrifuging small-volume samples fixed on an angle rotor, this invention places the sample inside a tube, then places the tube inside a sleeve. The outer edge of the tube secures the tube to the opening of the sleeve. The cap is screwed onto the surface of the sleeve using a screw groove and thread. The nitrile and fluororubber gaskets prevent the solution inside the tube from flowing out. Because the inner wall of the tube is coated with a polytetrafluoroethylene superhydrophobic coating, liquid residue on the inner wall is reduced during use. During centrifugation, the filtrate filtered through the filter membrane flows through the guide channel to the bottom of the support plate, and then flows into the inside of the sleeve along the bottom guide channel.

[0014] 2. In this invention, when the centrifuged sample is taken out from inside the sleeve and placed inside the base for weighing, the base can weigh small-volume samples, which is convenient for placing the tube during the weighing process. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0016] Figure 2 This is one of the partial cross-sectional schematic diagrams of the present invention;

[0017] Figure 3 This is a second partial cross-sectional schematic diagram of the present invention;

[0018] Figure 4 This is a partial cross-sectional schematic diagram of the present invention;

[0019] Figure 5 For the present invention Figure 2 An enlarged diagram of A in the diagram.

[0020] Legend:

[0021] 1. Tube; 2. Sleeve; 3. Outer edge; 4. Cap; 5. Threaded groove; 6. Base; 7. Nitrile gasket; 8. Fluororubber gasket; 9. Filter membrane; 10. Flow guide groove; 11. Silicone strip; 12. Support plate; 13. Annular microgroove. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0023] See Figures 1 to 5 As shown, the present invention provides a bacterial cell wet weight measuring device for a fixed angle rotor, including a tube 1, a sleeve 2 on the outside of the tube 1, an outer edge 3 fixedly connected to the top of the tube 1, a screw groove 5 on the surface of the sleeve 2, a filter membrane 9 on the inner wall of the tube 1, a support plate 12 on the outside of the tube 1, a flow guide groove 10 between the support plate 12 and the filter membrane 9, a silicone strip 11 at the bottom of the inner wall of the tube 1, a base 6 on the surface of the tube 1, a polytetrafluoroethylene superhydrophobic coating on the inner wall of the tube 1, volume scale lines printed on the outside of the tube 1, the filter membrane 9 being conical and inclined, and the silicone strip 11 being disposed between the filter membranes 9.

[0024] In an optional embodiment: the top of the sleeve 2 is provided with a cap 4, the inner wall of the cap 4 is provided with threads, a nitrile gasket 7 is fixedly connected to the inner wall of the cap 4, a fluororubber gasket 8 is fixedly connected to the bottom of the nitrile gasket 7, and an annular microgroove 13 is provided between the fluororubber gasket 8 and the inner wall of the cap 4.

[0025] It should be noted that when centrifuging small volumes of bacterial cells, the tube 1 with the filter membrane 9 should be weighed and recorded as x. Then, it should be placed in the sleeve 2 for collecting the supernatant. The culture medium containing the bacterial cells should be thoroughly mixed to avoid sampling errors caused by uneven bacterial distribution or precipitation. A precise volume of bacterial solution should be measured and placed into the tube 1 with the filter membrane 9. The cap 4 should then be closed and tightened. The nitrile gasket 7, the fluororubber gasket 8, and the annular microgroove 13 ensure a seal when the cap 4 is tightened, preventing liquid overflow and avoiding leakage of the solution inside the tube 1 during centrifugation. The container should then be placed in the fixed-angle rotor of the centrifuge. The centrifugation speed and time should be set according to the bacterial characteristics. The centrifuge should be started for centrifugation. After centrifugation, the cap 4 should be opened, and the tube 1 with the filter membrane 9 should be removed and weighed, recording as y. The weight of the tube 1 with the filter membrane 9 measured before and after centrifugation should be recorded. The wet weight of the bacteria, yx, is calculated. After centrifugation, the inner wall of tube 1 is coated with a polytetrafluoroethylene superhydrophobic coating, which reduces the amount of liquid residue on the inner wall during use. This reduces the impact of residual moisture on the weight of the bacteria and prevents large measurement errors. The filtrate inside tube 1 can be filtered out through the filter membrane 9 and flows through the guide channel 10 to the bottom of the support plate 12. It then flows from the guide channel 10 at the bottom of the support plate 12 into the inside of the sleeve 2 for separation. Since the filter membrane 9 is conical, it can quickly filter out the filtrate in the solution. A silicone strip 11 is set at the conical angle of the filter membrane 9. The silicone strip 11 completely fills the conical angle space. The upper surface of the silicone strip 11 is higher than the lower end of the filter membrane. This design can prevent the sample from remaining at the angle during centrifugation, ensuring that there is no residue at the angle between the silicone strip 11 and the filter membrane 9, thus keeping the inside of tube 1 clean.

[0026] After the bacterial solution has been centrifuged, once the filtrate inside tube 1 has completely flowed into the sleeve 2, open the cap 4, remove tube 1 from the sleeve 2, and place it inside the base 6 for weighing. This prevents tube 1 from tilting during weighing, which could cause the bacterial cells inside tube 1 to spill out and lead to inaccurate weighing. At the same time, the base 6 makes it very convenient to weigh tube 1.

[0027] In an optional embodiment: the filter membrane 9 is made of polyethersulfone, with a pore size ranging from 0.1 to 0.45 μm, preferably 0.22 μm, and a thickness of 0.05 to 0.2 mm, and is welded to the support plate 12 by laser welding technology.

[0028] It should be noted that polyethersulfone has excellent heat resistance, physical and mechanical properties, and insulation properties. In particular, it has outstanding advantages such as continuous use at high temperatures and stable performance in environments with rapid temperature changes. Therefore, there will be no change in pore size after centrifugation, which avoids the situation where bacteria flow out with the filtrate during filtration.

[0029] In an optional embodiment: the inside of the pipe 1 is provided with multiple guide grooves 10, which are evenly arranged inside the pipe 1.

[0030] It should be noted that, with the help of multiple guide channels 10, the filtrate inside the tube 1 can be discharged quickly, thereby achieving the effect of rapid filtrate filtration.

[0031] In an optional embodiment: the side of the support plate 12 that is attached to the filter membrane 9 is the front side, and the side of the support plate 12 that is away from the filter membrane 9 is the back side.

[0032] It should be noted that since the support plate 12 has two sides, the guide channel 10 can be stably opened on one side of the support plate 12 to facilitate the filtration of the filtrate.

[0033] In an optional embodiment: a silicone strip 11 is disposed at a position where the filter membrane 9 forms a conical angle, the silicone strip 11 completely fills the space of the conical angle, and the upper surface of the silicone strip 11 is higher than the lower end of the filter membrane 9.

[0034] It should be noted that because the upper surface of the silica gel strip 11 is higher than the lower end of the filter membrane 9, the sample can be prevented from remaining at the corner during centrifugation, and there will be no residue inside the tube 1 when the bacterial cells are poured out.

[0035] Working principle: When centrifuging small volumes of bacteria, weigh tube 1 with filter membrane 9 and record the weight as x. Then place it in tube 2 for collecting the supernatant. Thoroughly mix the culture medium containing bacteria to avoid sampling errors caused by uneven distribution or precipitation. Accurately measure a certain volume of bacterial solution into tube 1 with filter membrane 9, then cover with cap 4 and tighten it. With the action of nitrile gasket 7, fluororubber gasket 8, and annular microgroove 13, the cap 4 can provide a seal when tightened to prevent liquid from overflowing. Place the container in the fixed-angle rotor of the centrifuge, set the centrifugation speed and time according to the characteristics of the bacteria, start the centrifuge, and perform centrifugation. After centrifugation, open cap 4, take out tube 1 with filter membrane 9, weigh it, and record the weight as y. Based on the weight of tube 1 with filter membrane 9 measured before and after centrifugation, the wet weight of the bacteria, i.e., yx, is calculated. After centrifugation, because the inner wall of tube 1 is coated with a polytetrafluoroethylene superhydrophobic coating, the liquid residue on the inner wall can be reduced during use. The filtrate inside tube 1 can be filtered out through filter membrane 9 and flows through guide channel 10 to the bottom of support plate 12, and from the guide channel 10 at the bottom of support plate 12 to the inside of sleeve 2 for separation. Since filter membrane 9 is conical, it can quickly filter out the filtrate in the solution. A silicone strip 11 is set at the conical angle of filter membrane 9. The silicone strip 11 completely fills the conical angle space. The upper surface of silicone strip 11 is higher than the lower end of filter membrane. This design can avoid sample residue at the angle during centrifugation, thus keeping the inside of tube 1 clean.

[0036] After the bacterial solution has been centrifuged, once the filtrate inside tube 1 has completely flowed into the sleeve 2, open the cap 4, remove tube 1 from the sleeve 2, and place it inside the base 6 for weighing. This prevents tube 1 from tilting during weighing, and the base 6 makes it easy to weigh tube 1.

[0037] Finally, it should be noted that the above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A device for measuring the wet weight of bacterial cells using a fixed-angle rotor, comprising a tube (1), characterized in that: The tube (1) has a sleeve (2) on its outer side, an outer edge (3) fixedly connected to the top of the tube (1), a screw groove (5) on the surface of the sleeve (2), a filter membrane (9) on the inner wall of the tube (1), a support plate (12) on the outer side of the tube (1), a guide groove (10) between the support plate (12) and the filter membrane (9), a silicone strip (11) at the bottom of the inner wall of the tube (1), a base (6) on the surface of the tube (1), a polytetrafluoroethylene superhydrophobic coating on the inner wall of the tube (1), volume scale lines printed on the outer side of the tube (1), the filter membrane (9) is conical and inclined, and the silicone strip (11) is arranged between the filter membranes (9).

2. The bacterial cell wet weight measuring device for a fixed-angle rotor according to claim 1, characterized in that: The top of the sleeve (2) is provided with a cap (4), the inner wall of the cap (4) is provided with threads, a nitrile gasket (7) is fixedly connected to the inner wall of the cap (4), a fluororubber gasket (8) is fixedly connected to the bottom of the nitrile gasket (7), and an annular microgroove (13) is provided between the fluororubber gasket (8) and the inner wall of the cap (4).

3. The bacterial cell wet weight measuring device for a fixed-angle rotor according to claim 1, characterized in that: The filter membrane (9) is made of polyethersulfone with a pore size ranging from 0.1 to 0.45 μm, preferably 0.22 μm, and a thickness of 0.05 to 0.2 mm. It is welded to the support plate (12) by laser welding technology.

4. The bacterial cell wet weight measuring device for a fixed-angle rotor according to claim 1, characterized in that: The tube (1) is provided with multiple guide grooves (10) inside, and the multiple guide grooves (10) are evenly arranged inside the tube (1).

5. The bacterial cell wet weight measuring device for a fixed-angle rotor according to claim 1, characterized in that: The side of the support plate (12) that is in contact with the filter membrane (9) is the front side, and the side of the support plate (12) that is away from the filter membrane (9) is the back side.

6. The bacterial cell wet weight measuring device for a fixed-angle rotor according to claim 1, characterized in that: A silicone strip (11) is provided at the position of the filter membrane (9) at the conical angle. The silicone strip (11) completely fills the space of the conical angle, and the upper surface of the silicone strip (11) is higher than the lower end of the filter membrane (9).

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