一种脂质体佐剂中3D-MLA的定量检测方法

By employing acid hydrolysis and fluorescence detection, the challenge of detecting 3D-MLA content in HA201 liposome adjuvant was solved, enabling rapid and accurate quantitative detection, simplifying sample processing, making it suitable for large-scale sample testing, and ensuring stable product quality.

CN122171720BActive Publication Date: 2026-07-17BEIJING HUANUOTAI BIOMEDICAL TECH CO LTD
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Patent Information

Application Number
CN202610646300.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-05-12
Publication Date
2026-07-17
Estimated Expiration
2046-05-12

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Abstract

本申请涉及疫苗质量检测的技术领域，具体涉及一种脂质体佐剂中3D‑MLA的定量检测方法。具体包括以下步骤：取供试品溶液，加入盐酸进行酸水解，使3D‑MLA完全水解为D‑葡萄糖胺和D‑葡萄糖胺‑6‑磷酸；向水解后的样品中加入内标溶液和衍生试剂，进行衍生化反应，使糖胺和内标分别转化为具有荧光特性的衍生物；采用高效液相色谱‑荧光检测器对衍生后的样品进行检测；以葡萄糖胺总峰面积与内标峰面积的比值为依据，通过标准曲线法计算供试品中3D‑MLA的含量。本申请提供的检测方法专属性强、灵敏度高、操作简便且适配大批量样本。
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Citation Information

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