Astaxanthin composition for relieving visual fatigue and preparation method and application thereof

Abstract

The present application relates to a kind of astaxanthin composition that can relieve visual fatigue and its preparation method and application, belong to astaxanthin technical field, composition includes the following mass fraction components: astaxanthin oil 75-80 parts, xanthophyll ester 22-30 parts, cistanches extract 25-30 parts, roxburgh rose extract 42-55 parts, glyceryl monocaprylate 9-10 parts, β-cyclodextrin 35-40 parts, sodium carboxymethyl cellulose 18-52 parts.The astaxanthin of the present application, xanthophyll ester, cistanches extract and roxburgh rose extract compound can effectively improve the efficacy of astaxanthin relieving visual fatigue.

Description

Technical Field

[0001] This invention belongs to the field of astaxanthin technology, specifically, it relates to an astaxanthin composition that can relieve visual fatigue, its preparation method and application. Background Technology

[0002] Visual fatigue (asthenopia), also known as eye strain, is a series of uncomfortable eye symptoms caused by prolonged close-range use of the eyes or staring at screens. The main symptoms include blurred vision, dry and itchy eyes, photophobia, tearing, headaches, and eye soreness. With the rapid development of information technology and the widespread use of smart devices, people's daily screen time has increased dramatically. Statistics show that the global prevalence of myopia continues to rise, and it is estimated that by 2050, nearly half of the population will be affected by myopia. Among school-aged children, daily screen time generally exceeds 3-4 hours, with digital eye strain being particularly prominent. Long-term visual fatigue not only affects daily work and study efficiency but may also progress to more serious eye diseases, posing a significant threat to public visual health.

[0003] Photooxidative damage is considered one of the important molecular mechanisms underlying visual fatigue. When the retinal pigment epithelium is continuously exposed to high-energy visible light such as blue light, it produces a large number of reactive oxygen species (ROS), which trigger oxidative stress, leading to retinal cell damage and even apoptosis. Therefore, using exogenous antioxidants to scavenge free radicals and reduce retinal oxidative damage has become an important intervention for alleviating visual fatigue.

[0004] Astaxanthin is a ketocarotenoid pigment derived from microalgae such as Haematococcus pluvialis and marine organisms such as shrimp and crab shells, possessing extremely strong antioxidant activity. Studies have shown that astaxanthin's free radical scavenging ability far exceeds that of common antioxidants such as vitamin C, vitamin E, and beta-carotene. More importantly, astaxanthin has a unique amphiphilic molecular structure, enabling it to penetrate the blood-retinal barrier and directly reach and accumulate in ocular tissues such as the macula of the retina, exerting antioxidant and anti-inflammatory protective effects in the ocular microenvironment.

[0005] Numerous clinical studies have confirmed the clear effect of astaxanthin in relieving visual fatigue. In an 84-day randomized, double-blind, placebo-controlled trial, school-aged children who supplemented with 4 mg of natural astaxanthin daily showed a 20% decrease in chronic computer vision syndrome scores, a 27% improvement in visual fatigue scale scores, and significant improvements in stereopsis and tear secretion compared to the placebo group. Other studies have shown that after 28 days of continuous astaxanthin administration, uncorrected distance vision significantly improved in both the 4 mg and 12 mg groups, and ciliary muscle accommodation time was significantly shortened. Clinical studies on middle-aged and elderly individuals have also shown that astaxanthin can effectively reduce visual oxidative stress induced by screen time. These studies fully demonstrate that astaxanthin has outstanding application potential in relieving visual fatigue and improving visual function.

[0006] However, the effect of using astaxanthin alone to improve visual fatigue is limited. How to enhance the effect of astaxanthin in relieving visual fatigue by scientifically compounding natural plant extracts is a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0007] In view of the shortcomings of the prior art, the present invention provides an astaxanthin composition that can relieve visual fatigue, its preparation method and application.

[0008] To achieve the above-mentioned objectives, the present invention provides the following technical solution: The first aspect of the present invention provides an astaxanthin composition that can relieve visual fatigue, comprising the following components in parts by weight: 75-80 parts astaxanthin oil, 22-30 parts lutein ester, 25-30 parts broom flower extract, 42-55 parts prickly pear extract, 9-10 parts caprylic acid glyceride, 35-40 parts β-cyclodextrin, and 18-52 parts sodium carboxymethyl cellulose.

[0009] Further, the product contains the following components in parts by weight: 78 parts astaxanthin oil, 25 parts lutein ester, 28 parts broom flower extract, 48 parts prickly pear extract, 9.5 parts caprylic acid glyceride, 37 parts β-cyclodextrin, and 35 parts sodium carboxymethyl cellulose.

[0010] A second aspect of the present invention provides a method for preparing the combination described in the first aspect, comprising the following steps: S1, add glyceryl monocaprylate to astaxanthin oil and lutein ester, stir to emulsify, and obtain an emulsion; S2, add sodium carboxymethyl cellulose and 1 / 4-1 / 2 β-cyclodextrin to the emulsion, and stir evenly to obtain a mixed slurry; S3, spray dry the mixed slurry obtained in step S2 to obtain astaxanthin powder; S4, add broom flower extract, prickly pear extract and the remaining β-cyclodextrin to an appropriate amount of purified water and stir to dissolve, then spray dry the mixture to obtain a mixed powder; S5. The astaxanthin powder obtained in step S3 and the mixed powder obtained in step S4 are mixed evenly to obtain the composition.

[0011] The third aspect of the present invention provides the composition obtained in the second aspect.

[0012] The fourth aspect of the present invention provides the use of the composition of the third aspect in the preparation of a medicament for relieving visual fatigue. Beneficial effects

[0013] This invention combines astaxanthin, lutein ester, broom flower extract and prickly pear extract to effectively enhance the effect of astaxanthin in relieving visual fatigue. Detailed Implementation

[0014] The present invention will be described in detail below with reference to the embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0015] In this invention, the astaxanthin oil is extracted by our company (Yunnan Green A Biotechnology Co., Ltd.), and the astaxanthin oil contains 5% astaxanthin. The broom flower extract was purchased from Fufeng Sinote Biotechnology Co., Ltd., and it is a water extract with a ratio of 10:1. The prickly pear extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd. It is a water extract of prickly pear fruit with a specification of 10:1. Caprylic / caprylic glyceride was purchased from Hebei Hongtao Bioengineering Co., Ltd. β-Cyclodextrin was purchased from Chongqing Jiulingling Biotechnology Co., Ltd. Sodium carboxymethyl cellulose was purchased from Hebei Pengyu Biotechnology Co., Ltd. Lutein esters were purchased from Guangdong Mingcheng Biotechnology Co., Ltd. Example 1

[0016] An astaxanthin composition that can relieve visual fatigue comprises the following components in parts by weight: 78 parts astaxanthin oil, 26 parts lutein ester, 28 parts broom flower extract, 48 parts prickly pear extract, 9.5 parts caprylic acid glyceride, 37 parts β-cyclodextrin, and 35 parts sodium carboxymethyl cellulose.

[0017] The preparation method of the combination includes the following steps: S1, add glyceryl monocaprylate to astaxanthin oil and lutein ester, stir to emulsify, and obtain an emulsion; S2, add sodium carboxymethyl cellulose and 1 / 2 β-cyclodextrin to the emulsion, and stir evenly to obtain a mixed slurry; S3, spray dry the mixed slurry obtained in step S2 to obtain astaxanthin powder; S4, add broom flower extract, prickly pear extract and the remaining β-cyclodextrin to an appropriate amount of purified water and stir to dissolve, then spray dry the mixture to obtain a mixed powder; S5. The astaxanthin powder obtained in step S3 and the mixed powder obtained in step S4 are mixed evenly to obtain the composition. Example 2

[0018] An astaxanthin composition that can relieve visual fatigue comprises the following components in parts by weight: 75 parts astaxanthin oil, 22 parts lutein ester, 25 parts broom flower extract, 42 parts prickly pear extract, 9 parts caprylic acid glyceride, 35 parts β-cyclodextrin, and 18 parts sodium carboxymethyl cellulose.

[0019] A second aspect of the present invention provides a method for preparing the combination described in the first aspect, comprising the following steps: S1, add glyceryl monocaprylate to astaxanthin oil and lutein ester, stir to emulsify, and obtain an emulsion; S2, add sodium carboxymethyl cellulose and 1 / 4 of β-cyclodextrin to the emulsion, and stir evenly to obtain a mixed slurry; S3, spray dry the mixed slurry obtained in step S2 to obtain astaxanthin powder; S4, add broom flower extract, prickly pear extract and the remaining β-cyclodextrin to an appropriate amount of purified water and stir to dissolve, then spray dry the mixture to obtain a mixed powder; S5. The astaxanthin powder obtained in step S3 and the mixed powder obtained in step S4 are mixed evenly to obtain the composition. Example 3

[0020] An astaxanthin composition that can relieve visual fatigue comprises the following components in parts by weight: 80 parts astaxanthin oil, 30 parts lutein ester, 30 parts broom flower extract, 55 parts prickly pear extract, 10 parts caprylic acid glyceride, 40 parts β-cyclodextrin, and 52 parts sodium carboxymethyl cellulose.

[0021] A second aspect of the present invention provides a method for preparing the combination described in the first aspect, comprising the following steps: S1, add glyceryl monocaprylate to astaxanthin oil and lutein ester, stir to emulsify, and obtain an emulsion; S2, add sodium carboxymethyl cellulose and 1 / 2 β-cyclodextrin to the emulsion, and stir evenly to obtain a mixed slurry; S3, spray dry the mixed slurry obtained in step S2 to obtain astaxanthin powder; S4, add broom flower extract, prickly pear extract and the remaining β-cyclodextrin to an appropriate amount of purified water and stir to dissolve, then spray dry the mixture to obtain a mixed powder; S5. The astaxanthin powder obtained in step S3 and the mixed powder obtained in step S4 are mixed evenly to obtain the composition. Comparative Example 1

[0022] The difference between Comparative Example 1 and Example 1 is that Comparative Example 1 does not contain broom flower extract, and broom flower extract is replaced with prickly pear extract. Comparative Example 2

[0023] The difference between Comparative Example 2 and Example 1 is that it does not contain prickly pear extract, but instead replaces prickly pear extract with broom extract.

[0024] Experimental Example 1

[0025] Experimental study on the effect of the composition of the present invention on a dry eye model Sixty SPF-grade male BALB / c mice, weighing between 18 and 20 g, were selected and acclimatized for one week. Six mice were randomly selected as the normal control group, while the other 54 mice were given 0.2% benzalkonium chloride solution in both eyes to establish a mouse model of dry eye. 5 μL was instilled into each eye three times daily for seven consecutive days. The success of the model was observed and evaluated using fluorescein staining.

[0026] In addition to the normal control group (saline), 36 mice that successfully developed the model were randomly selected and divided into 6 groups. The grouping and drug dosage are as follows: Normal group (physiological saline), model control group (physiological saline), groups 1-3 (corresponding to the administration of the compositions of Examples 1, 2, and 3 above, respectively), and groups 4-5 (corresponding to the administration of the compositions of Comparative Examples 1-2 above, respectively).

[0027] Dosage: The recommended daily intake of astaxanthin for adults is 4-12 mg / day; based on 12 mg / day, this is calculated. Astaxanthin oil contains 5% astaxanthin, requiring an intake of 240 mg of astaxanthin oil. Assuming an adult weight of 60 kg, the recommended intake of astaxanthin oil for adults is 4 mg / kg body weight (BW). The dosage for mice in groups 1-5 was then calculated using this baseline. The normal control group and the model control group were given the same volume of distilled water, and mice in each group were administered the medication by gavage for 28 consecutive days.

[0028] 2. Indicator Measurement 2.1 Measurement of tear secretion According to the reference method (Wu SQ, Dai Q, Xu JL, et al. Combined effect of traditional Chinese and Western medicine on inflammatory factors in patients with diabetes-induced xerophthalmia[J]. Genetics and Molecular Research, 2016, 15(4): 15-17.), the phenol red cotton thread soaking method was used to detect the amount of tear secretion in mice before and after drug administration, and the length of the cotton thread that turned red was measured.

[0029] 2.2 Determination of tear film breakup time The tear film breakup time in mice before and after drug administration was detected by fluorescein staining method (KIM J,KIM JY,SEO KY, et al. Location and pattern of non-invasive keratographic tear film breakup according to dry eyedisease subtypes[J].Acta Ophthalmologica,2019,97(10):324-331.).

[0030] Table 1 Effects of each treatment on tear secretion Example 1 7.74±0.91 Example 2 7.59±1.02 Example 3 7.88±1.10 Comparative Example 1 5.72±0.65 Comparative Example 2 5.80±0.93 normal group 8.73±0.42 Model control group 3.51±0.58 As can be seen from Table 1 above, the combination of astaxanthin oil, broom extract and prickly pear extract prepared by the present invention can effectively increase tear secretion.

[0031] Table 2. Effects of each treatment on tear film breakup time Example 1 5.12±0.50 Example 2 5.07±0.62 Example 3 5.22±0.71 Comparative Example 1 3.96±0.85 Comparative Example 2 4.09±0.79 normal group 5.41±0.46 Model control group 1.40±0.31 As can be seen from Table 2 above, the combination of astaxanthin oil, lutein ester, broom extract and prickly pear extract prepared by the present invention can effectively improve tear film breakup time.

[0032] In summary, the composition of the present invention can significantly increase tear secretion and prolong tear film breakup time, and has a significant therapeutic effect on dry eye syndrome.

[0033] Experimental Example 2

[0034] Visual persistence test of the composition of the present invention Trial administration experimental steps (1) Conduct relevant questionnaires for qualified volunteers to conduct preliminary screening.

[0035] (2) The volunteers are required to take a visual persistence test, and volunteers with a visual persistence of ≤90% are eligible to participate in the composition trial administration test and are identified as subjects.

[0036] (3) The subjects start taking the composition prepared in Example 1, once a day (the dosage is converted according to the subject's body weight, based on the adult intake of astaxanthin oil: 4mg / kg BW), and one course of treatment lasts 10 days.

[0037] (4) After the subjects have taken the composition for three courses of treatment, the visual persistence test is performed.

[0038] Steps of the visual persistence test A "pin"-shaped (Chinese character pin) three-dimensional block pattern is drawn on the inspection table, each side of the block is 1cm long, and the local illumination is 100LX-150LX (a specially made light box can be used). During the measurement, the distance between the inspection table and the eyes should be kept at an appropriate distance according to the subject's viewing habit without moving. It is stipulated that the subject seeing the "pin" character image is regarded as clear vision, and seeing the inverted "pin" character is regarded as unclear vision. The measurement time is 3 minutes.

[0039] During the inspection, the subject is asked to hold a stopwatch capable of intermittent timing. After the inspector gives the start command, the subject immediately gazes at the pattern in the block (or turns on the light box switch), and starts the stopwatch in hand at the same time to time. During the gazing process, when the inverted "pin" character is seen, press the pause switch of the stopwatch immediately; when the "pin" character image is seen again, restart the stopwatch, and repeat this process.

[0040] When the specified 3-minute measurement time ends, the subject hears the inspector's command and immediately stops the stopwatch. The reading on the stopwatch during this period is the total time the subject sees the "pin" character image, that is, the clear vision time.

[0041] Visual persistence = (clear vision time / total gazing time) × 100%.

[0042] Attention should be paid to the site and illumination during the measurement, and the result is also related to the degree of eye use of the subject before the test, which should be noted before the experiment.

[0043] 3. The experimental results are shown in Table 3.

[0044] 1 54.27 67.30 13.03 2 61.38 85.84 24.46 3 57.92 67.20 9.28 4 53.45 73.88 20.43 5 55.78 71.09 15.31 6 60.14 78.20 18.06 7 53.47 68.12 14.65 8 63.28 71.91 8.63 9 58.63 73.27 14.64 10 56.91 69.93 13.02 11 62.75 81.06 18.31 12 59.33 74.72 15.39 13 65.21 77.80 12.59 14 53.56 69.29 15.73 15 60.44 76.64 16.20 16 55.03 69.03 14.00 17 57.41 79.08 21.67 18 62.09 75.24 13.15 19 64.76 80.06 15.30 20 58.15 77.81 19.66 21 54.89 65.34 10.45 22 61.63 70.26 8.63 23 56.24 78.05 21.81 24 53.98 71.52 17.54 25 59.70 76.93 17.23

[0045] Table 3 Experimental results of the visual persistence of the composition of the present invention As can be seen from Table 3 above, 22 out of 25 subjects had their visual persistence improved to varying degrees (an improvement of less than 10% is not counted), with an effective rate of 88%.

[0046] The above description is merely a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the forms disclosed herein and should not be construed as excluding other embodiments. It can be used in various other combinations, modifications, and environments, and can be altered within the scope of the concept described herein through the above teachings or related technologies or knowledge. Modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention should be within the protection scope of the appended claims.

Claims

1. An astaxanthin composition that can relieve visual fatigue, characterized in that: The composition comprises the following components in parts by weight: 75-80 parts astaxanthin oil, 22-30 parts lutein ester, 25-30 parts broom flower extract, 42-55 parts prickly pear extract, 9-10 parts caprylic / caprylic acid glyceride, 35-40 parts β-cyclodextrin, and 18-52 parts sodium carboxymethyl cellulose; the preparation method of the composition includes the following steps: S1, add glyceryl monocaprylate to astaxanthin oil and lutein ester, stir to emulsify, and obtain an emulsion; S2, add 1 / 4 to 1 / 2 of the total mass of sodium carboxymethyl cellulose and β-cyclodextrin to the emulsion, and stir evenly to obtain a mixed slurry; S3, spray dry the mixed slurry obtained in step S2 to obtain astaxanthin powder; S4, add broom flower extract, prickly pear extract and the remaining β-cyclodextrin to an appropriate amount of purified water and stir to dissolve, then spray dry the mixture to obtain a mixed powder; S5. The astaxanthin powder obtained in step S3 and the mixed powder obtained in step S4 are mixed evenly to obtain the composition.

2. The astaxanthin composition for relieving visual fatigue according to claim 1, characterized in that: It contains the following components in parts by weight: 78 parts astaxanthin oil, 25 parts lutein ester, 28 parts broom flower extract, 48 parts prickly pear extract, 9.5 parts caprylic acid glyceride, 37 parts β-cyclodextrin, and 35 parts sodium carboxymethyl cellulose.

3. The use of the composition according to claim 1 in the preparation of a medicament for relieving visual fatigue.