一种氟苯尼考和甲砜霉素单克隆抗体的重链和轻链可变区及其应用

By preparing the heavy and light chain variable regions of florfenicol and thiamphenicol monoclonal antibodies for use in colloidal gold test strips, the residual problem in the detection of florfenicol and thiamphenicol was solved, and an efficient, safe and rapid detection method was achieved.

CN122187988BActive Publication Date: 2026-07-17北京纳百生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京纳百生物科技有限公司
Filing Date
2026-05-15
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing detection methods for florfenicol and thiamphenicol are prone to improper use, leading to residues and accumulation in animals and endangering human health. Furthermore, there is a lack of efficient and safe biological detection methods.

Method used

We provide the heavy and light chain variable regions of florfenicol and thiamphenicol monoclonal antibodies for the preparation of detection products such as colloidal gold test strips, enabling rapid detection using highly specific and sensitive monoclonal antibodies.

Benefits of technology

It achieves high sensitivity and specificity for the detection of florfenicol and thiamphenicol, is suitable for rapid detection of large numbers of samples, reduces detection costs and improves detection efficiency.

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Abstract

本发明公开了一种氟苯尼考和甲砜霉素单克隆抗体及其应用,属于生物技术领域。所述单克隆抗体含有重链可变区和轻链可变区,所述重链可变区和轻链可变区均由互补决定区和框架区组成,所述互补决定区均由CDR1、CDR2和CDR3组成。所述单克隆抗体可用于制备氟苯尼考和甲砜霉素检测制品。本发明所提供的氟苯尼考和甲砜霉素胶体金检测试纸条,具有灵敏度高、特异性好、稳定性强的特点。
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Citation Information

Patent Citations

  • Method for detecting florfenicol and florfenicol amine and special-purpose enzyme-linked immunologic reagent kit thereof

    CN101299045A

  • Monoclonal antibody for detecting thiamphenicol and florfenicol, ELISA (enzyme-linked immunosorbent assay) method and kit

    CN105524174A