A complex culture system microbial contamination closed-loop monitoring system and method
By designing a gradient spiral banding system and a transparent trapping cavity, combined with closed-loop reflux and in-situ monitoring, the problems of low abundance of target microorganisms and background interference in complex culture systems are solved, achieving efficient detection and monitoring of microbial contamination.
CN122188783APending Publication Date: 2026-06-12NANJING NORMAL UNIVERSITY
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- NANJING NORMAL UNIVERSITY
- Filing Date
- 2026-05-18
- Publication Date
- 2026-06-12
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Figure CN122188783A_ABST
Abstract
The application discloses a complex culture system microbial contamination closed-loop monitoring system and method, and belongs to the technical field of microfluidic sample pretreatment and process monitoring. The system comprises a gradient spiral zoning module, an online lateral entrapment module, a closed-loop reflux module, an auxiliary pumping and liquid supplementing module and an in-situ monitoring module. Through continuous zoning, cyclic enrichment and lateral entrapment of different scale components in a complex sample, preferential retention and in-situ monitoring of low-abundance microbial contaminants are realized. The application does not need complex labeling and is suitable for contamination early warning and pretreatment of cell culture process liquid, fermentation process liquid, biological process sampling liquid and other complex suspension system samples.
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