贝壳杉烯酸13-羟化酶及在全发酵产甜菊糖苷中的应用
By mutating kaurene acid 13-hydroxylase to G481L/Q159L, its catalytic efficiency was improved, solving the problem of low catalytic efficiency in steviol glycoside production. This enabled the efficient production of steviol and steviol glycosides and reduced production costs.
CN122188954BActive Publication Date: 2026-07-17SICHUAN INGIA BIOSYNTHETIC CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SICHUAN INGIA BIOSYNTHETIC CO LTD
- Filing Date
- 2026-05-18
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
In existing methods for producing steviol glycosides, the catalytic efficiency of kauronic acid 13-hydroxylase (KAH) is low, which affects the synthesis efficiency of steviol and steviol glycosides, leading to issues with yield and cost.
Method used
By mutating wild-type kaurenoic acid 13-hydroxylase to G481L/Q159L, a highly active KAH mutant was constructed, and a steviol or steviol glycoside synthesis pathway was constructed in Saccharomyces cerevisiae to improve the expression level and catalytic efficiency of key enzymes.
Benefits of technology
The molar conversion rate of kaurene acid to steviol catalyzed by the KAH mutant increased from 70.5% to 96.8%, and the total fermentation yield of Reb M increased from 25.812 mg/L to 52.247 mg/L, while reducing production costs.
✦ Generated by Eureka AI based on patent content.
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Abstract
本发明涉及生物合成技术领域，特别涉及贝壳杉烯酸13‑羟化酶及在全发酵产甜菊糖苷中的应用。本发明利用定点突变技术对野生型贝壳杉烯酸13‑羟化酶进行突变，获得G481L / Q159L突变体。本发明的KAH突变体相较于野生型具有更高的活性，催化贝壳杉烯酸合成甜菊醇的摩尔转化率自70.5%提升至96.8%；KAH突变体对Reb M的全发酵生产产生了极显著的影响，其产量自25.812mg / L显著提升到了52.247mg / L。可见，G481L / Q159L突变体可应用于甜菊醇或全发酵生产甜菊糖苷的高效生产中，为工业化生产降低成本。
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