Method for constructing leptin gene modified obese golden hamster model and application thereof

By constructing a leptin-deficient model in golden hamsters using CRISPR-Cas9 technology, this study addresses the problem that existing animal models cannot simulate obesity-related SARS-CoV-2 infection in humans, providing an efficient research platform for the study of obesity-related SARS-CoV-2 infection mechanisms and drug development.

CN122189089APending Publication Date: 2026-06-12NANJING MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING MEDICAL UNIV
Filing Date
2026-01-09
Publication Date
2026-06-12

AI Technical Summary

⚠Technical Problem

Existing animal models, such as mice, cannot effectively simulate the severe symptoms and metabolic characteristics of obesity-related SARS-CoV-2 infection in humans, which increases the difficulty of studying the pathogenesis of obesity-related SARS-CoV-2 infection.

⚗Method used

By injecting Cas9 protein and sgRNA targeting exon 2 of the Leptin gene into two-cell embryos under red light using CRISPR-Cas9 technology, a Leptin-deficient golden hamster model was constructed to simulate obesity-related severe SARS-CoV-2 infection.

🎯Benefits of technology

It provides an animal model that more closely resembles human metabolic characteristics, enabling effective research into obesity-related SARS-CoV-2 infection mechanisms, screening of therapeutic drugs, and development of vaccines. Furthermore, the model construction method is efficient and has a stable reproductive transmission rate.

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Abstract

The application discloses a construction method of a Leptin gene modified obese golden hamster model and application thereof, and the sgRNA sample prepared by targeting the hamster Leptin gene is microinjected into a single two-cell stage embryo blastocyst, and then is transferred into a real pregnant surrogate to develop in vivo after culture recovery, so that the hamster Leptin gene can be accurately targeted and modified, and a genetically modified hamster is obtained. The two-cell stage embryo gene editing which overcomes the hamster embryonic development arrest period can overcome the embryonic development arrest caused by in vitro operation, and the litter effect is stable. The golden hamster can be directly infected with SARS-CoV-2 and show similar pathological characteristics to humans, and the ob / ob golden hamster model will fill the blank of a severe model animal of a novel coronavirus infection, and plays an important role in the fields of infection mechanism research, treatment drug screening and new pathogenic virus strain evaluation of SARS-CoV-2.
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