A method for increasing the protein content of soybean seeds

Abstract

This invention discloses a method for increasing the protein content of soybean seeds. The method involves supplementing soybean plants with molybdenum during the soybean growth cycle. This supplementation is achieved by applying a molybdenum-containing compound solution to the leaves of the soybean plants. The concentration of the molybdenum-containing compound in the solution is greater than 0.1% by mass and less than 0.4% by mass. The molybdenum compound includes one or more of molybdate, molybdenum oxide, or molybdenum-containing phosphate. The molybdenum supplementation is performed at least at one of the following stages of soybean growth: seedling stage, budding stage, or flowering stage. This method can increase the protein content of soybean seeds, ensure stable agronomic traits and yield, and provide technical support for high-quality and high-yield soybean cultivation.

Description

Technical Field

[0001] This invention relates to a method for increasing the protein content of soybean seeds, belonging to the field of crop cultivation technology. Background Technology

[0002] In soybean cultivation, the protein content of soybean seeds is one of the key indicators for measuring their quality and economic value. Improving the protein content of soybean seeds is of great significance for meeting the needs of the food industry (such as the application of soy protein in dairy processing) and the feed industry. Currently, my country's soybean production faces an urgent need for quality improvement, and cultivation techniques are one of the important factors affecting soybean quality.

[0003] In existing technologies, there are various cultivation methods to increase the protein content of soybean seeds, including optimizing fertilization structure (such as adjusting the nitrogen, phosphorus, and potassium ratio), improving planting density, and selecting high-quality varieties. Among these, there are numerous reports on the effects of micronutrients on soybean quality. For example, the "Specifications and Data Standards for Soybean Germplasm Resource Description" standardizes the methods for determining soybean agronomic traits and quality indicators, but it does not cover specific technical solutions for spraying specific micronutrients to increase protein content. Reference 1, "Research on the Effects of Micronutrients on Soybean Yield and Quality," points out that spraying micronutrients such as zinc, boron, and molybdenum can affect soybean quality to some extent, but systematic research on the specific concentrations and spraying times for increasing soybean seed protein content is relatively lacking.

[0004] Reference 2 proposes improving soybean quality through basal application of molybdenum fertilizer, but this method suffers from low molybdenum utilization and slow results, and the optimal application concentration and timing are not clearly defined. Currently, there is also a lack of specialized technical solutions on the market for spraying specific concentrations of ammonium molybdate onto soybeans to precisely increase grain protein content.

[0005] References

[0006] Reference 1: Wu Tuo, Yang Liu, Jiang Zhibing. Effects of trace elements molybdenum, zinc and boron on soybean yield and quality. Southern Agriculture, 2015, 9(31): 6-8.

[0007] Reference 2: CN102257916A Summary of the Invention

[0008] The problem the invention aims to solve

[0009] Although existing technologies utilize the application of molybdenum fertilizer to promote soybean growth, the limitations imposed by the method of application are still quite evident. Through extensive practical experience, the applicant of this invention has further discovered the following drawbacks of existing technologies in increasing the protein content of soybean seeds:

[0010] 1. Inappropriate application of molybdenum fertilizer: Current technology mostly adopts the method of basal application of molybdenum fertilizer. Molybdenum is easily fixed in the soil, resulting in low crop absorption and utilization rate. It cannot be effectively applied to the key growth period of soybeans and it is difficult to significantly increase the protein content of grains.

[0011] 2. Lack of precise concentration and timing control: The exploration of molybdenum spraying concentration and timing is relatively fragmented, and the optimal spraying concentration for the two key growth stages of soybean seedling and budding has not been clearly defined, resulting in unstable improvement effects. Some schemes even affect soybean growth due to inappropriate concentration.

[0012] 3. Poor overall results: Some technical solutions only focus on improving a single indicator, ignoring the impact on other agronomic traits of soybeans (such as plant height and grain weight per plant) and yield, making it difficult to effectively improve protein content while ensuring stable yield.

[0013] To address the aforementioned shortcomings, this invention provides a highly efficient molybdenum fertilizer application method. By applying the fertilizer through foliar spraying, molybdenum is directly absorbed by soybean leaves, thereby improving the utilization rate of molybdenum. Furthermore, it clarifies the optimal concentration of ammonium molybdate for application during the soybean seedling and budding stages, achieving precise enhancement of soybean grain protein content.

[0014] The method provided by this invention can increase the protein content of soybean seeds while ensuring the stability of soybean agronomic traits and that yield is not negatively affected, thus providing technical support for high-quality and high-yield soybean cultivation.

[0015] Solution for solving the problem

[0016] [1]. A method for increasing the protein content of soybeans, wherein, during the growth cycle of soybeans, molybdenum is supplemented to soybean plants by applying a solution containing a molybdenum compound to the soybean plants, and the concentration of the molybdenum compound in the solution is greater than 0.1% by mass and less than 0.4% by mass, wherein the molybdenum compound includes one or more of molybdate, molybdenum oxide or molybdenum-containing phosphate, and the molybdenum supplementation is carried out at least during one of the seedling stage, budding stage or flowering stage of soybean growth.

[0017] [2]. According to the method described in [1], the molybdenum-containing compound includes at least one of ammonium molybdate, sodium molybdate, potassium molybdate, calcium molybdate, molybdenum trioxide, and molybdenum-containing superphosphate.

[0018] [3]. According to the method described in [1] or [2], wherein the amount of solution sprayed each time is not less than 100 kg / hm. 2 ,

[0019] Preferably, the amount of solution sprayed each time is 150-200 kg / hm².

[0020] [4]. The method according to any one of [1]-[3], wherein the spraying is performed on the leaves of the soybean plant.

[0021] [5]. The method according to any one of [1]-[4], wherein the spraying is performed at least once during any period of 20-40 days after soybean sowing, 40-60 days after soybean sowing, or 60-80 days after soybean sowing.

[0022] [6]. The method according to any one of [1]-[5], wherein the molybdenum supplementation is applied to the front and back of the soybean plant leaves.

[0023] [7]. The method according to any one of [1]-[6], wherein the seed density of the soybean at the time of sowing is not less than 250,000 plants / hm².

[0024] [8]. Application of molybdenum-containing foliar fertilizer in increasing soybean yield and / or improving grain protein content, wherein the concentration of molybdenum in the foliar fertilizer is above 0.1% by mass and less than 0.4% by mass.

[0025] [9]. Application of molybdenum-containing foliar fertilizer in the preparation of compositions for increasing soybean yield and / or improving grain protein content, wherein the concentration of molybdenum in the foliar fertilizer is greater than 0.1% by mass and less than 0.4% by mass.

[0026]

[10] . According to the application described in [8] or [9], the molybdenum-containing foliar fertilizer is applied to the leaves of soybean plants at least once during one of the seedling, budding or flowering stages of the soybean growth cycle.

[0027] The effects of the invention

[0028] This invention clarifies the optimal spraying concentration ratio and application timing for the two key growth stages of seedling and budding, overcoming the problems of scattered and unstable concentration and timing in existing technologies, ensuring the consistency and reliability of spraying effects, and avoiding negative impacts on soybean growth due to improper concentration.

[0029] This invention not only significantly improves the protein content of soybean seeds, but also takes into account the improvement of traits such as plant height and single-plant grain weight, effectively improving soybean quality while ensuring stable or even increased soybean yield, overcoming the shortcomings of previous technologies that only focused on a single indicator and neglected comprehensive benefits.

[0030] The spraying method provided by this invention is clear and the parameters are specific, making it easy to operate in the field and promote in a standardized manner. It helps to stably increase protein content and ensure yield in soybean cultivation, and has good practical application prospects and industrial value. Attached Figure Description

[0031] Figure 1 This is a diagram illustrating the soybean growth stages. Detailed Implementation

[0032] Various exemplary embodiments, features, and aspects of the present invention will be described in detail below. The term "exemplary" as used herein means "serving as an example, embodiment, or illustration." Any embodiment described herein as "exemplary" is not necessarily to be construed as superior to or better than other embodiments.

[0033] Furthermore, to better illustrate the present invention, numerous specific details are set forth in the following detailed embodiments. Those skilled in the art should understand that the present invention can be practiced without certain specific details. In other instances, methods, means, apparatus, and steps well known to those skilled in the art have not been described in detail in order to highlight the spirit of the present invention.

[0034] Unless otherwise stated, all units used in this specification are international standard units, and all numerical values ​​and ranges appearing in this invention should be understood to include systematic errors that are unavoidable in industrial production.

[0035] In this specification, the word "may" has two meanings: to perform a certain process and not to perform a certain process.

[0036] In this specification, references to "some specific / preferred embodiments," "other specific / preferred embodiments," "implementation," etc., refer to specific elements (e.g., features, structures, properties, and / or characteristics) related to that embodiment, which are included in at least one of the embodiments described herein and may or may not be present in other embodiments. Furthermore, it should be understood that these elements may be combined in any suitable manner in various embodiments.

[0037] In this specification, the range of values ​​referred to as "value A to value B" refers to the range including the endpoint values ​​A and B.

[0038] <First Aspect>

[0039] This invention provides a method for increasing the protein content of soybeans. During the soybean growth cycle, molybdenum is supplemented to the soybean plants by applying a solution containing a molybdenum compound to the leaves of the soybean plants. The concentration of the molybdenum compound in the solution is greater than 0.1% by mass and less than 0.4% by mass. The molybdenum compound includes one or more of molybdate, molybdenum oxide, or molybdenum-containing phosphate. The molybdenum supplementation is carried out at least during one of the seedling stage, budding stage, or flowering stage of soybean growth.

[0040] In some exemplary embodiments, the soybean variety described in this invention is the "BWYR-01" variety.

[0041] (sowing)

[0042] In some implementations, the seed density at the time of sowing is not less than 250,000 plants / hm², and preferably, it can be 250,000 to 350,000 plants / hm².

[0043] In some exemplary implementations, compound fertilizer (45% effective ingredient, N:P:K = 15:15:15) is applied at sowing, at a rate of 30 kg / mu, and the soil is cultivated and weeded three times.

[0044] (cultivation)

[0045] In some exemplary implementations, cultivation management includes randomized block design, 3 replicates, 6 rows, row length 5m, row spacing 0.6m, and sowing density of 320,000 plants / hm².

[0046] In some exemplary implementations, a plot of land with flat terrain and uniform soil fertility is selected, preferably with light black calcareous soil, and an average temperature of 15-16℃, rainfall of 550-570 mm, and sunshine duration of 1350-1400 h during the growing season to ensure normal soybean growth.

[0047] (Compound)

[0048] In some embodiments, the molybdenum-containing compound is not particularly limited in principle and can be a molybdenum-containing fertilizer raw material. In this invention, the molybdenum compound may include one or more of molybdates, molybdenum oxides, or molybdenum-containing phosphates.

[0049] In some specific embodiments, the molybdenum-containing compound may include at least one of ammonium molybdate, sodium molybdate, potassium molybdate, calcium molybdate, molybdenum trioxide, and molybdenum-containing superphosphate.

[0050] In some preferred embodiments, the molybdenum-containing compound includes ammonium molybdate.

[0051] In some preferred embodiments, the ammonium molybdate includes at least one of ammonium orthomolybdate, ammonium dimolybdate, ammonium paramolybdate, ammonium tetramolybdate, and their hydrated forms.

[0052] In some exemplary embodiments, the ammonium molybdate comprises ammonium heptamolybdate tetrahydrate [(NH4)6Mo7O 24 ·4H2O).

[0053] In some exemplary embodiments, ammonium heptamolybdate tetrahydrate [(NH4)6Mo7O] is used. 24 [·4H2O] dissolves in water.

[0054] In some more preferred embodiments, the concentration of the molybdenum compound in the solution is above 0.1% by mass and below 0.3% by mass, for example, 0.1% by mass, 0.15% by mass, 0.2% by mass, 0.25% by mass, 0.3% by mass, etc.

[0055] (Solution)

[0056] In some implementations, molybdenum supplementation is carried out by dissolving a molybdenum-containing compound in a solvent and applying it, especially by spraying.

[0057] The present invention does not have any particular limitation on the solvent, and can be a commonly used solvent in the art or a liquid foliar fertilizer. In some exemplary embodiments, the solvent of the present invention is water.

[0058] In some implementations, there is no particular limitation on the amount of solution sprayed each time when supplementing molybdenum. However, to better obtain the effects of the present invention, the amount of solution sprayed each time should not be less than 100 kg / hm². 2 .

[0059] In some preferred embodiments, when supplementing molybdenum, the amount of solution sprayed each time is 150-200 kg / hm².

[0060] In some preferred embodiments, when supplementing molybdenum, the amount of solution sprayed each time is 160-190 kg / hm², such as 165 kg / hm², 170 kg / hm², 175 kg / hm², 180 kg / hm², 185 kg / hm², 190 kg / hm², etc.

[0061] In some exemplary embodiments, when supplementing molybdenum, the amount of solution sprayed each time is 175-185 kg / hm².

[0062] In some implementations, the molybdenum supplementation is applied by spraying both the front and back of the soybean plant leaves.

[0063] In some exemplary implementations, the spraying process ensures that both sides of the leaves are evenly covered with the pesticide, and the amount of spray should be such that the leaves are moistened but not dripping wet.

[0064] In some implementations, the spraying is performed on the leaves of the soybean plant.

[0065] In some implementations, the spraying time is no less than one day.

[0066] The timing of molybdenum supplementation according to the present invention can be carried out at least during one of the seedling stage, budding stage, and flowering stage of soybean growth.

[0067] See appendix Figure 1It provides a schematic diagram of the soybean growth stages, which can be divided into three stages: the vegetative growth stage (stage I), the stage of simultaneous vegetative and reproductive growth (stage II), and the reproductive growth stage (stage III).

[0068] in:

[0069] Phase I can include the seedling stage (VE), cotyledon stage (VC), first node stage (V1), and second node stage (V2).

[0070] Phase II may include the initial flowering period (R1), full bloom period (R2), initial pod formation period (R3), full pod formation period (R4), initial grain formation period (R5), and grain filling period (R6).

[0071] Stage III can include early maturity (R7) and full maturity (R8).

[0072] Furthermore, the "seedling stage" mentioned in this invention can be the emergence stage (VE), cotyledon stage (VC), first node stage (V1), and second node stage (V2) mentioned above. Preferably, the "seedling stage" of this invention can be the cotyledon stage (VC), first node stage (V1), and second node stage (V2) mentioned above. Since the plant has already produced leaves, it is more conducive to the application of the molybdenum compound solution.

[0073] The “budding period” mentioned in this invention can be a period from the second stage (V2) to the beginning of flowering (R1) (excluding the beginning of flowering R1).

[0074] The "flowering period" of this invention can be the initial flowering period (R1) and the full bloom period (R2) mentioned above.

[0075] Furthermore, in some preferred embodiments of the present invention, the timing of molybdenum supplementation can be at least during the period from the cotyledon stage (VC) to the full bloom stage (R1).

[0076] In addition, in terms of timing, in some other specific embodiments of the present invention, molybdenum supplementation is carried out at least during one of the seedling or budding stages of soybean growth. This can be achieved by spraying a molybdenum-containing compound solution at least once during one or more of the following periods: 20-40 days after soybean sowing, 40-60 days after soybean sowing, or 60-80 days after soybean sowing.

[0077] In some preferred embodiments, foliar spraying can be carried out at least once each during the soybean seedling stage, budding stage, and flowering stage.

[0078] In a further preferred embodiment:

[0079] The soybean seedling stage is between 25 and 40 days after sowing.

[0080] The budding stage of soybeans is between 45 and 55 days after sowing.

[0081] The flowering period of soybeans is between 60 and 80 days after sowing.

[0082] In some other preferred embodiments, the spraying time includes at least one combination of the following:

[0083] Apply foliar spray at least once during the soybean seedling stage (30-35 days after sowing) and the budding stage (40-60 days after sowing);

[0084] Apply foliar spray at least once during the soybean seedling stage (30-35 days after sowing);

[0085] Apply foliar spray at least once during the soybean budding stage (40-60 days after sowing) and at least once during the full flowering stage (60-80 days after sowing);

[0086] Apply foliar spray at least once during the soybean seedling stage (30-35 days after sowing), budding stage (40-60 days after sowing), and flowering stage (60-80 days after sowing).

[0087] In some preferred embodiments, the present invention has found that foliar spraying at least once during the soybean seedling stage, i.e., 30 to 33 days after sowing, has a better effect on improving seed protein content.

[0088] In some exemplary embodiments, the spraying is performed at least once during the soybean seedling stage (32 days after sowing) and the budding stage (55 days after sowing);

[0089] Apply at least once only during the soybean seedling stage (32 days after sowing);

[0090] Apply the spray at least once only during the soybean budding stage (55 days after sowing);

[0091] Apply the spray at least once during the soybean budding stage (55 days after sowing) and at the flowering stage (70 days after sowing).

[0092] In some further preferred embodiments, a 0.2% ammonium molybdate solution is prepared and applied as a foliar spray at least once during the soybean seedling stage (32 days after sowing) and the budding stage (55 days after sowing), with each application amounting to 180 kg / hm².

[0093] Using the method described above in this invention can increase soybean yield and improve the protein content of the seeds.

[0094] <Second aspect>

[0095] A second aspect of the present invention provides a foliar fertilizer for increasing soybean yield and / or improving seed protein content, wherein the concentration of molybdenum in the foliar fertilizer is greater than 0.1% by mass and less than 0.4% by mass.

[0096] The present invention also provides the application of molybdenum-containing foliar fertilizer in increasing soybean yield and / or improving grain protein content, characterized in that the concentration of molybdenum in the foliar fertilizer is above 0.1% by mass and less than 0.4% by mass.

[0097] The present invention also provides the application of molybdenum-containing foliar fertilizer in the preparation of compositions for increasing soybean yield and / or improving seed protein content, characterized in that the concentration of molybdenum in the foliar fertilizer is above 0.1% by mass and less than 0.4% by mass.

[0098] In some specific implementation schemes, molybdenum-containing foliar fertilizers may contain, in addition to molybdenum compounds, the following components: nitrogen, phosphorus, and potassium elements essential for soybean growth; magnesium elements to enhance photosynthesis; iron elements to regulate plant redox reactions and improve crop absorption of nutrients; and trace elements and amino acids required for soybean growth.

[0099] In some specific implementations, soybeans grown using the method described in this invention have a protein content greater than 35% and a yield greater than 3630 kg / hm². 2 The soybean grain weight per plant is greater than 19.4 g, and the soybean plant height is less than 94 cm.

[0100] In some preferred embodiments, soybeans grown using the method described in this invention have a protein content greater than 35.2%, for example: 35.2%, 35.25%, 35.3%, 35.35%, 35.4%, 35.45%, etc.

[0101] In some exemplary embodiments, soybeans grown using the method described in this invention have a protein content of 35.21%, 36.16%, 35.58%, 35.45%, 35.68%, and 35.82%.

[0102] In some preferred embodiments, soybeans grown using the method described in this invention have a soybean protein content of 36% or higher.

[0103] In some preferred embodiments, soybeans grown using the method described in this invention yield more than 3640 kg / hm². 2 For example: 3641 kg / hm 2 3645 kg / hm 2 3650 kg / hm 2 3651 kg / hm 2 wait.

[0104] In some exemplary embodiments, soybeans grown using the method described in this invention achieve a yield of 3662.5 kg / hm². 2 3676.3 kg / hm 2 3651.8 kg / hm 2 3658.2 kg / hm 2 3665.7 kg / hm 2 3669.1 kg / hm 2 .

[0105] In some preferred embodiments, soybeans grown using the method described in this invention achieve a yield of 3670 kg / hm². 2 above.

[0106] In some preferred embodiments, soybeans grown using the method described in this invention have a single plant grain weight greater than 19.45 g, for example: 19.46 g, 19.47 g, 19.48 g, 19.49 g, 19.50 g, etc.

[0107] In some exemplary embodiments, soybeans grown using the method described in this invention have individual plant grain weights of 19.80 g, 20.15 g, 19.50 g, 19.78 g, 19.98 g, and 20.09 g.

[0108] In some preferred embodiments, soybeans grown using the method described in this invention have a single soybean grain weight greater than 20 g.

[0109] In some preferred embodiments, soybeans grown using the method described in this invention have a plant height greater than 83 cm, for example: 85 cm, 85.5 cm, 86 cm, 86.5 cm, 87 cm, 87.5 cm, 88 cm, 88.5 cm, etc.

[0110] In some exemplary embodiments, soybeans grown using the method described in this invention have plant heights of 88.50 cm, 86.98 cm, 85.30 cm, 87.21 cm, 86.25 cm, and 86.37 cm.

[0111] In some more preferred embodiments, soybeans grown using the method described in this invention have a plant height between 86.5 and 87.5 cm.

[0112] Example

[0113] The embodiments of the present invention will be described in detail below with reference to examples. However, those skilled in the art will understand that the following examples are for illustrative purposes only and should not be considered as limiting the scope of the invention. Unless otherwise specified in the examples, conventional conditions or conditions recommended by the manufacturer are followed. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0114] This invention involves foliar spraying a specific concentration of ammonium heptamolybdate tetrahydrate [(NH4)6Mo7O] at a specific growth stage of soybeans. 24 A solution containing ·4H2O was used to increase the protein content of soybean seeds. The materials and methods are as follows:

[0115] Experimental materials: Soybean varieties suitable for local planting (such as the "BWYR-01" variety in this experiment); ammonium heptamolybdate tetrahydrate [(NH4)6Mo7O 24 [·4H2O], purity ≥99%.

[0116] Experimental site selection: Select a flat plot of land with uniform soil fertility. The soil type should be light black calcareous soil. The previous crop should be corn. The average temperature during the growing season should be 15-16℃, the rainfall should be 550-570 mm, and the sunshine duration should be 1350-1400h to ensure normal soybean growth.

[0117] Basic cultivation management: A randomized block design with 3 replicates, 6 rows per plot, row length 5 m, row spacing 0.6 m, and sowing density of 320,000 plants / hm² was adopted. Compound fertilizer (45% effective ingredient, N:P:K = 15:15:15) was mechanically applied at sowing at a rate of 30 kg / mu. Three rounds of cultivation and weeding were performed; other management practices were the same as in conventional field cultivation.

[0118] Preparation and application of ammonium molybdate solution: Ammonium heptamolybdate tetrahydrate [(NH4)6Mo7O] 24 Dissolve ·4H2O in water to prepare a 0.2% ammonium molybdate solution. Apply the solution as a foliar spray during the soybean seedling, budding, and flowering stages. Ensure that both sides of the leaves are evenly coated during spraying. The amount of spray should be enough to moisten the leaves but not drip water. The amount of spraying per application is 150-200 kg / hm².

[0119] Detection indicators and methods: Referring to the "Specifications and Data Standards for Description of Soybean Germplasm Resources", soybean seeds were harvested at maturity, and the protein content of soybean seeds was determined by the Kjeldahl method. At the same time, agronomic traits and yield indicators such as soybean plant height, stem diameter, single plant seed weight, and yield per hectare were measured to evaluate the impact of the technical scheme on the overall growth of soybean.

[0120] Detection methods

[0121] Soybean seed protein content: The Kjeldahl nitrogen determination method was used, and the Kjeldahl nitrogen analyzer (model Kjeltec 8400) was used for detection.

[0122] Agronomic traits were measured as follows: plant height was measured by measuring the distance from the base to the top of the plant with a tape measure; grain weight per plant was measured by weighing the harvested grain weight of a single plant using an electronic balance (accuracy 0.01 g); yield per hectare was calculated by measuring the grain in the middle 4 rows (yield measurement area 12 m²) of the harvest plot and converting it into yield per hectare.

[0123] Molybdenum utilization rate: The amount of molybdenum accumulated in soybean plants was determined by inductively coupled plasma mass spectrometry (ICP-MS), and the molybdenum utilization rate was calculated in combination with the total amount of ammonium molybdate applied.

[0124] Example 1

[0125] (1) Experimental materials: Soybean variety “BWYR-01”; Ammonium heptamolybdate tetrahydrate [(NH4)6Mo7O 24 ·4H2O] (purity 99.5%).

[0126] (2) Experimental site: Agricultural Science and Technology Park of Heilongjiang North Latitude 47 Green Organic Food Co., Ltd. (Yian County, Heilongjiang Province, N:47°55′, E:125°15′), the soil is light black calcareous soil, the previous crop is corn, the average temperature during the growing season is 15.1℃, the precipitation is 560.4 mm, and the sunshine duration is 1377.7 h.

[0127] (3) Basic cultivation management: randomized block design, 3 replicates, 6 rows per block, row length 5 m, row spacing 0.6 m, sowing density 320,000 plants / hm². Apply compound fertilizer (45% effective ingredient, N:P:K = 15:15:15) at sowing, fertilizer amount 30 kg / mu, and cultivate and weed 3 times.

[0128] (4) Spraying with ammonium molybdate solution: Prepare an ammonium molybdate solution with a concentration of 0.1% and spray it on the leaves during the soybean seedling stage (32 days after sowing) and the budding stage (55 days after sowing). The amount of spraying each time is 180 kg / hm².

[0129] (5) The soybean seeds planted in this embodiment have a protein content of 35.21 g / 100g, a plant height of 88.5 cm, a single plant grain weight of 19.8 g, and a yield of 3662.5 kg per hectare.

[0130] Example 2

[0131] (1) The experimental materials, location and basic cultivation management are the same as in Example 1.

[0132] (2) Spraying with ammonium molybdate solution: Prepare an ammonium molybdate solution with a concentration of 0.2% and spray it on the leaves during the soybean seedling stage (32 days after sowing) and the budding stage (55 days after sowing). The amount of spraying each time is 180 kg / hm².

[0133] (3) The soybean seeds planted using this method have a protein content of 36.16 g / 100g, a plant height of 86.98 cm, a single plant grain weight of 20.15 g, and a yield of 3676.3 kg per hectare.

[0134] Example 3

[0135] (1) The experimental materials, location and basic cultivation management are the same as in Example 1.

[0136] (2) Spraying with ammonium molybdate solution: Prepare an ammonium molybdate solution with a concentration of 0.3% and spray it on the leaves during the soybean seedling stage (32 days after sowing) and the budding stage (55 days after sowing). The amount of spraying each time is 180 kg / hm².

[0137] (3) The soybean seeds planted in this embodiment have a protein content of 35.58 g / 100g, a plant height of 85.3 cm, a single plant grain weight of 19.5 g, and a yield of 3651.8 kg per hectare.

[0138] Example 4

[0139] (1) The experimental materials, location and basic cultivation management are the same as in Example 1.

[0140] (2) Ammonium molybdate solution spraying: Prepare an ammonium molybdate solution with a concentration of 0.2% and spray it only once during the soybean seedling stage (32 days after sowing). The spraying amount is 180 kg / hm².

[0141] (3) The soybean seeds planted in this embodiment have a protein content of 35.45 g / 100g and a yield of 3658.2 kg per hectare.

[0142] Example 5

[0143] (1) The experimental materials, location and basic cultivation management are the same as in Example 1.

[0144] (2) Spraying with ammonium molybdate solution: Prepare an ammonium molybdate solution with a concentration of 0.2% and spray it on the leaves only once during the soybean budding stage (55 days after sowing). The spraying amount is 180 kg / hm².

[0145] (3) The soybean seeds planted in this embodiment have a protein content of 35.68 g / 100g and a yield of 3665.7 kg per hectare.

[0146] Example 6

[0147] (1) The experimental materials, location and basic cultivation management are the same as in Example 1.

[0148] (2) Spraying with ammonium molybdate solution: Prepare an ammonium molybdate solution with a concentration of 0.2% and spray it on the leaves once each during the soybean budding stage (55 days after sowing) and flowering stage (70 days after sowing), with a spraying amount of 180 kg / hm².

[0149] (3) The soybean seeds planted in this embodiment have a protein content of 35.82 g / 100g and a yield of 3669.1 kg per hectare.

[0150] Comparative Example 1

[0151] (1) The experimental materials, location and basic cultivation management are the same as in Example 1.

[0152] (2) Spraying treatment: Spray water (ammonium molybdate-free) on the leaves of soybeans during the seedling and budding stages, at a rate of 180 kg / hm².

[0153] (3) The soybean seeds grown using this method have a protein content of 34.54 g / 100g, a plant height of 94.08 cm, a single plant grain weight of 19.34 g, and a yield of 3667.8 kg per hectare.

[0154] The method in Comparative Example 1 did not show a significant improvement in the protein content of soybeans grown using this method.

[0155] Comparative Example 2

[0156] (1) The experimental materials, location and basic cultivation management are the same as in Example 1.

[0157] (2) Spraying with ammonium molybdate solution: Prepare an ammonium molybdate solution with a concentration of 0.05% and spray it on the leaves during the soybean seedling and budding stages. The amount of each spraying is 180 kg / hm².

[0158] (3) The soybean seeds planted in this implementation plan have a protein content of 34.87 g / 100g, a plant height of 90.2 cm, a single plant grain weight of 19.4 g, and a yield of 3658.6 kg per hectare.

[0159] The concentration of ammonium molybdate used in Comparative Example 2 was too low, resulting in a small increase in the protein content of the soybeans grown.

[0160] Comparative Example 3

[0161] (1) The experimental materials, location and basic cultivation management are the same as in Example 1.

[0162] (2) Ammonium molybdate solution spraying: Prepare an ammonium molybdate solution with a concentration of 0.4% and spray it on the leaves during the soybean seedling and budding stages. The amount of each spraying is 180 kg / hm².

[0163] (3) The soybean seeds planted using this method have a protein content of 35.02 g / 100g, some leaves show signs of scorching, plant height is 83.7 cm, single plant grain weight is 18.9 g, and yield per hectare is 3623.4 kg.

[0164] The method in Comparative Example 3 used an excessively high concentration of ammonium molybdate, resulting in poor protein enhancement in the planted soybeans, significantly reduced plant height, and negatively impacted soybean growth.

[0165] Table 1. Data on soybean protein content, yield, and plant traits.

[0166]

[0167] The above embodiments and comparative examples are summarized in Table 1:

[0168] Regarding protein content, the overall soybean protein content of Examples 1-6 was higher than that of the comparative group, with Example 2 reaching 36.16%, an increase of 4.69% compared to Comparative Example 1 (34.54%).

[0169] In terms of single-plant grain weight, Examples 1-6 were generally greater than 19.5 g, which was better than the comparative group.

[0170] In terms of yield, Examples 1-6 showed overall stability, all within a reasonable fluctuation range of 3650-3680 kg / hm². This indicates that the fertilization method of the present invention can still maintain a high yield. However, for Comparative Example 3 (0.4% high concentration), the leaf burn was caused by the excessively high concentration, and the yield was significantly reduced to 3623.4 kg / hm², further confirming the rationality and necessity of the concentration range (0.1%-0.3%) of the present invention.

[0171] Regarding plant height, Comparative Example 1 reached the highest at 94.08 cm, but its protein content was only 34.54%, the lowest among all treatments. Comparative Example 2 had a plant height of 90.20 cm and a protein content of 34.87%, also significantly lower than the Example group. This indicates that simply pursuing plant height growth is not conducive to protein accumulation and may even inhibit grain protein synthesis due to excessive vegetative growth. In contrast, Examples 1-6 controlled plant height within a reasonable range of 85-89 cm. By moderately controlling vegetative growth, more photosynthetic products were allocated towards protein synthesis, achieving a synergistic effect of increasing both protein content and yield.

[0172] Furthermore, in terms of single-plant grain weight, the comparative group was significantly lower than the example group, meaning that excessively long or short plant height both affected single-plant grain weight. In summary, the method provided by this invention ensures that plant height and yield are within the normal range, and through improved methods, further accumulates single-plant grain weight and coordinates grain protein and other qualities to achieve a synergy between high yield and high quality.

[0173] It should be emphasized that the protein content of Comparative Examples 1 and 2 is significantly lower than that of the embodiments of the present invention, which precisely confirms the technical understanding that "simply pursuing vegetative growth cannot achieve quality improvement" and also highlights the superiority of the technical solution of the present invention in coordinating the relationship between growth and quality.

[0174] Furthermore, the present invention (1) significantly increases the protein content of soybean seeds: compared with the control treatment sprayed with water (Comparative Example 1), the protein content of soybean seeds in the best implementation scheme (Example 2) of this technical solution is increased from 34.54 g / 100g to 36.16 g / 100g, an increase of 4.69%, which meets the food industry's demand for high-protein soybean raw materials.

[0175] (2) Ensure stable soybean agronomic traits and yield: While increasing protein content, the soybean plant height, single plant grain weight, and yield per hectare did not decrease significantly compared with the control. The yield per hectare in Example 2 was 3676.3 kg, which was slightly higher than the control's 3667.8 kg, achieving a balance between high quality and high yield.

[0176] (3) Improve the utilization rate of molybdenum: The foliar spraying method avoids the fixation of molybdenum by the soil. The molybdenum is directly absorbed and utilized by the soybean leaves, and its utilization rate is significantly improved compared with the traditional soil fertilization method, which further promotes the increase of soybean grain protein content.

[0177] (4) The combination of spraying periods has a synergistic effect: Through systematic comparative experiments on different periods, it has been proven that the "seedling stage + budding stage" spraying combination selected in this invention, at the same concentration and dosage, has a significantly better effect on increasing the protein content of soybean seeds than other single periods (seedling stage only or budding stage only) or other period combinations (such as budding stage + flowering stage). This indicates that the key growth periods synergistically promote the absorption of molybdenum and protein synthesis.

[0178] (5) Simple operation and controllable cost: This technical solution only requires two foliar sprays during the soybean seedling stage and the budding stage. It is simple to operate and easy to promote. The amount of ammonium molybdate used is small, and the concentration is controlled within the range of 0.1%-0.3%. The production cost is low and it is suitable for large-scale field application.

[0179] It should be noted that although the technical solution of the present invention has been described with specific examples, those skilled in the art will understand that the present invention should not be limited thereto.

[0180] The various embodiments of the present invention have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or technical improvements to the embodiments in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A method for increasing the protein content of soybeans, characterized in that, During the soybean growth cycle, molybdenum is supplemented to the soybean plants by applying a molybdenum compound solution to the plants, wherein the concentration of the molybdenum compound in the solution is greater than 0.1% by mass and less than 0.4% by mass. The molybdenum-containing compound includes one or more of molybdates, molybdenum oxides, or molybdenum-containing phosphates. The molybdenum supplementation is performed at least during one of the following stages of soybean growth: seedling stage, budding stage, or flowering stage.

2. The method according to claim 1, characterized in that, The molybdenum-containing compound includes at least one of ammonium molybdate, sodium molybdate, potassium molybdate, calcium molybdate, molybdenum trioxide, and molybdenum-containing superphosphate.

3. The method according to claim 1 or 2, characterized in that, The amount of solution sprayed each time should not be less than 100 kg / hm. 2 .

4. The method according to any one of claims 1-3, characterized in that, The spraying is applied to the leaves of the soybean plants.

5. The method according to any one of claims 1-4, characterized in that, Apply the spray at least once at any time during the following periods: 20-40 days after soybean sowing, 40-60 days after soybean sowing, and 60-80 days after soybean sowing.

6. The method according to any one of claims 1-5, characterized in that, The molybdenum supplement is applied by spraying both the front and back of the soybean plant leaves.

7. The method according to any one of claims 1-6, characterized in that, The soybean seed density at the time of sowing shall not be less than 250,000 plants / hm².

8. The application of molybdenum-containing foliar fertilizer in increasing soybean yield and / or improving grain protein content, characterized in that, The concentration of molybdenum in the foliar fertilizer is above 0.1% by mass and less than 0.4% by mass.

9. The application of molybdenum-containing foliar fertilizer in the preparation of compositions for increasing soybean yield and / or improving grain protein content, characterized in that, The concentration of molybdenum in the foliar fertilizer is above 0.1% by mass and less than 0.4% by mass.

10. The application according to claim 8 or 9, characterized in that, Molybdenum-containing foliar fertilizer should be applied to the leaves of soybean plants at least once during one of the seedling, budding, or flowering stages of the soybean growth cycle.

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