An injectable self-healing hydrogel and a preparation method thereof

By dynamically cross-linking Schiff bases through oxidized astragalus polysaccharide and lysozyme, an injectable self-healing hydrogel was prepared. This solved the problem of insufficient ability of lysozyme to kill Gram-negative bacteria and biofilms, and achieved efficient destruction of Gram-negative bacteria and biofilms and synergistic regulation of wound healing. It is suitable for irregular wounds and has injectability and self-healing properties.

CN122229995APending Publication Date: 2026-06-19GUANGDONG MEDICAL UNIV
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Patent Information

Application Number
CN202610324426.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-17
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

Existing lysozymes are insufficient in killing Gram-negative bacteria and biofilm-forming bacteria. Their single antibacterial function cannot regulate the wound healing microenvironment, and they are difficult to form into hydrogel dressings suitable for irregular wound surfaces.

Method used

By oxidizing the hydroxyl groups of Astragalus polysaccharide to active aldehyde groups, oxidized Astragalus polysaccharide is prepared and dynamically cross-linked with lysozyme to form Schiff bases, achieving self-assembly into an injectable self-healing hydrogel. This enhances the ability to destroy Gram-negative bacteria and biofilms, and combines the antioxidant and angiogenesis-promoting activities of Astragalus polysaccharide.

Benefits of technology

It achieves efficient destruction of Gram-negative bacteria and biofilms, optimizes the immune microenvironment, promotes wound healing, is suitable for irregular wounds, and has injectability and self-healing properties, meeting the requirements of green medical materials.

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Abstract

This invention relates to the field of biomedical technology, specifically to an injectable self-healing hydrogel and its preparation method. The preparation method includes the following steps: (1) Preparation of oxidized astragalus polysaccharide: The hydroxyl groups of astragalus polysaccharide are gently oxidized to active aldehyde groups to obtain oxidized astragalus polysaccharide; (2) Self-assembly of the injectable self-healing hydrogel: The oxidized astragalus polysaccharide and lysozyme are mixed evenly in PBS buffer at a certain mass ratio, and allowed to stand at room temperature for 3-5 minutes to self-assemble into an injectable self-healing hydrogel. The injectable self-healing hydrogel of this invention achieves the organic fusion of astragalus polysaccharide and lysozyme through gelation, and enhances the ability of lysozyme to destroy Gram-negative bacteria and biofilms through a synergistic strategy; it systematically elucidates the mechanism of action of lysozyme in clearing bacterial biofilms, regulating fibroblast proliferation and promoting chronic wound healing, providing a new theoretical paradigm and experimental basis for the synergistic regulation of tissue regeneration by natural active ingredients.
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