A gene chip for simultaneously detecting 12 foodborne pathogenic bacteria and application thereof

CN122235342APending Publication Date: 2026-06-19QUZHOU FUXIN MEDICAL TECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
QUZHOU FUXIN MEDICAL TECHNOLOGY CO LTD
Filing Date
2026-05-21
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

Existing technologies are insufficient for high-throughput, rapid, and accurate detection of multiple foodborne pathogens, and the imperfect gene chip preparation process leads to insufficient detection stability and repeatability.

Method used

Using polyvinyl chloride as a solid-phase carrier, combined with a 5' end PolyT modified probe and ultraviolet crosslinking technology, a gene chip for the simultaneous detection of 12 foodborne pathogens was prepared, and simultaneous detection was achieved through multiplex PCR amplification and hybridization detection procedures.

Benefits of technology

It enables simultaneous detection of 12 foodborne pathogens, improves detection throughput and stability, simplifies the process, reduces human error, and is suitable for rapid screening and determination of food safety incidents.

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Abstract

This invention relates to the field of biodetection technology, specifically disclosing a gene chip for the simultaneous detection of 12 foodborne pathogens and its applications. The gene chip includes a polyvinyl chloride solid-phase carrier and specific probes, positive control probes, and hybridization control probes immobilized on its surface. This gene chip can simultaneously detect Salmonella, Escherichia coli O157:H7, Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Vibrio parahaemolyticus, Vibrio cholerae, Vibrio fluvialis, Shigella flexneri, Campylobacter jejuni, Clostridium perfringens, and Yersinia enterocolitica. The gene chip of this invention employs a pre-defined dot matrix arrangement and achieves detection through total DNA extraction from the sample, multiplex PCR amplification and biotin labeling, chip hybridization, washing, blocking, enzyme labeling, color development, and scanning interpretation. It can achieve simultaneous detection of 12 target bacteria and has good specificity, repeatability, and application value.
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