Naringenin high-yield yeast engineering bacteria and application thereof

Abstract

The present application relates to a kind of high naringenin production yeast engineering bacteria and its application, belong to compound biosynthesis technical field.The present application is based on yeast expression system, the following four key genes: CHI gene from soybean, CHS gene from petunia hybrid, TAL gene from flavobacterium and 4CL gene from populus euphratica are respectively expressed as the protein of naringenin production yeast synthesis enzyme, measured engineering bacteria production naringenin titer reaches 60.5 mg / L.Further, by increasing copy number, enhancing precursor substrate expression, enhancing cofactor NADPH flux and other technical means, the yield of naringenin is improved to gram level, to solve the technical problem of low yield of naringenin synthesis in yeast in prior art.

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