Transdermal fibronectin and its preparation method and application

Abstract

The application discloses a transdermal fibronectin, a preparation method and application thereof. At least two fibronectin active domain modules are connected in series, a transdermal short peptide coding sequence is fused at the N end of each module, a pH-sensitive cleavage intein coding sequence is inserted between adjacent modules, and a fusion protein expression frame is constructed; a constitutive promoter P p311 The present application realizes high expression without induction in Lactobacillus plantarum; after separation and purification, the pH is adjusted to 5.0-5.5 to make the intein cleave, and multiple small molecule transdermal fibronectins are released. The present application does not need to add an exogenous inducer, does not produce endotoxin, avoids using an expensive enzyme cutting process, and realizes green and safe production. The obtained small molecule fibronectin has excellent transdermal property, cell migration promotion activity and skin repair effect, and can be used in the fields of anti-aging, soothing, repair and the like of cosmetics and the fields of wound repair and the like of medical devices.

Images