Special growth regulator for peanut and its application in sugarcane-peanut intercropping planting

Abstract

The present application relates to the technical field of microorganism, and particularly relates to a special growth regulator for peanuts and application of the special growth regulator for peanuts in sugarcane-peanut intercropping planting, the special growth regulator for peanuts comprises 1g / L-3g / L of GX-2-sugarcane leaf microbial growth regulator, 2g / L-6g / L of GX-7-peanut seedling microbial growth regulator, 0.2g / L-0.4g / L of brassinolide and 0.2g / L-0.4g / L of jasmonic acid, under the concentration, the special growth regulator for peanuts can significantly improve the yield of peanuts, under the condition of sugarcane-peanut intercropping, the special growth regulator for peanuts is more remarkable for improving the yield of peanuts, and the special growth regulator for peanuts can provide a new idea for developing special agricultural biological agents.

Description

Technical Field

[0001] This invention relates to the field of microbial technology, and in particular to a peanut-specific growth regulator and its application in sugarcane-peanut intercropping. Background Technology

[0002] *Streptococcus alactolyticus* refers to the vast majority of species in the genus *Streptococcus* except for *Streptococcus lactis*. In existing technology, *Streptococcus lactis*, which belongs to the same genus as *Streptococcus alactolyticus*, has been reported as a biofertilizer. For example, patent CN111574299A discloses *Streptococcus lactis* as a microbial agent used to prepare a biofertilizer for repairing the antioxidant system of plant leaves. Furthermore, existing technology reports that beneficial microorganisms in microbial agents can decompose organic matter in plant residues and organic fertilizers into soluble substances such as organic acids, amino acids, and sugars. These substances can be absorbed and utilized by plant roots, providing the nutrients needed by the plant. Existing technology also indicates that a mixture of succinic acid and lactic acid at a certain concentration can increase plant biomass. However, *Streptococcus alactolyticus* is not mentioned in existing technology. Reports of using *Alactolyticus* directly in the production of biofertilizers are based on the following: This strain is an animal-derived strain with limited research on plant fermentation, and it is highly unlikely to be suitable for soil improvement. However, during the preparation of animal feed using this strain, we discovered that it promotes the release of growth hormones from certain plants, specifically by forming indole compounds during fermentation. This has been corroborated in studies of the metabolomics of plants fermented by lactic acid bacteria. Furthermore, if certain plants naturally contain growth hormones, this strain can accumulate these hormones during fermentation and accelerate the metabolism of organic matter. This means we can utilize... The possibility of using fermentation products to prepare plant growth regulators that promote plant growth; there are related reports on the production of plant growth hormones in lactic acid bacteria that also produce acetic acid; in reports on the production of indoleacetic acid by lactic acid bacteria fermenting sauerkraut, existing technology reveals that not all lactic acid bacteria and plant fermentation will necessarily produce the corresponding effect. Therefore, this indicates that the enrichment of plant growth hormones is closely related to the strain and the plant substrate itself, and has a certain degree of specificity. In order to better study whether *Streptococcus non-lactolyticus* has the same effect on promoting plant growth after fermenting plants as other microorganisms, we must explore more microbial agents and discuss the possibility of their fermentation products in promoting plant growth. Summary of the Invention

[0003] In light of the above, in order to better investigate whether non-lactolytic streptococci, like other microorganisms, have the effect of promoting plant growth after fermenting plants, we must explore more microbial agents and discuss the possibility of their fermentation products promoting plant growth.

[0004] To achieve the above objective, the technical solution of the present invention is as follows.

[0005] A special growth regulator for peanuts, wherein the special growth regulator for peanuts consists of a microbial growth regulator and a dwarfing agent.

[0006] The microbial growth regulators are GX-2-sugarcane leaf microbial growth regulator and GX-7-peanut vine microbial growth regulator.

[0007] The dwarfing agents are brassinosteroid and jasmonic acid.

[0008] The GX-2-sugarcane leaf microbial growth regulator is prepared by inoculating *streptococcus alactolyticus* strain GX-2 into sugarcane leaf slurry, followed by fermentation, filtration and concentration.

[0009] The GX-7-peanut vine microbial growth regulator is prepared by inoculating *streptococcus alactolyticus* strain GX-7 into peanut vine slurry, followed by fermentation, filtration and concentration.

[0010] The strain information of said *streptococcus alactolyticus* strain GX-2 is: *streptococcus alactolyticus* GX-2, its taxonomic nomenclature is: *streptococcus alactolyticus*, the Chinese taxonomic nomenclature is: 非解乳糖链球菌, the deposit number is GDMCC NO: 65508, the depository authority is: Guangdong Microbial Culture Collection Center; the deposit address is: 5th Floor, Building 59, No. 100 Xianlie Middle Road, Institute of Microbiology, Guangdong Academy of Sciences; the deposit date is: November 19, 2024.

[0011] The strain information of said *streptococcus alactolyticus* strain GX-7 is: *streptococcus alactolyticus* GX-7, its taxonomic nomenclature is: *streptococcus alactolyticus*, the Chinese taxonomic nomenclature is: 非解乳糖链球菌, the deposit number is GDMCC NO: 65509, the depository authority is: Guangdong Microbial Culture Collection Center; the deposit address is: 5th Floor, Building 59, No. 100 Xianlie Middle Road, Institute of Microbiology, Guangdong Academy of Sciences; the deposit date is: November 19, 2024.

[0012] Furthermore, the peanut-specific growth regulator is composed of 1 g / L-3 g / L of GX-2-sugarcane leaf microbial growth regulator, 2 g / L-4 g / L of GX-7-peanut vine microbial growth regulator, 0.1 g / L-0.3 g / L of brassinolide, and 0.2 g / L-0.3 g / L of jasmonic acid.

[0013] Furthermore, the peanut-specific growth regulator is composed of 2 g / L of GX-2-sugarcane leaf microbial growth regulator, 2 g / L of GX-7-peanut vine microbial growth regulator, 0.2 g / L of brassinolide, and 0.3 g / L of jasmonic acid.

[0014] This invention also includes the application of the peanut-specific growth regulator in sugarcane-peanut intercropping. The method of sugarcane-peanut intercropping is as follows: after intercropping sugarcane stalks and peanut seeds, cultivate for 30 days. On the 30th day, spray a microbial growth regulator composed of GX-2-sugarcane leaf microbial growth regulator and GX-7-peanut vine microbial growth regulator, and mark this spraying as the first time. After that, spray once more after 15 days, for a total of 2 sprays of microbial growth regulator. On the third time, spray the peanut-specific growth regulator. After that, spray the peanut-specific growth regulator once every 15 days, with conventional field management, no additional fertilization, and harvest 120 days after sowing.

[0015] Furthermore, the peanut-specific growth regulator is composed of 1 g / L of GX-2-sugarcane leaf microbial growth regulator, 4 g / L of GX-7-peanut vine microbial growth regulator, 0.2 g / L of brassinolide, and 0.2 g / L of jasmonic acid.

[0016] This invention offers the following advantages: The microbial growth regulators of this invention are prepared by fermenting sugarcane leaves with *Streptococcus non-lactolyticus* strain GX-2 and peanut vines with *Streptococcus non-lactolyticus* strain GX-7. Experiments have verified that these regulators significantly promote peanut plant growth; however, they do not significantly increase peanut yield. Therefore, we combined these microbial growth regulators with dwarfing agents: brassinolide and jasmonic acid, to prepare a specialized growth regulator. Experiments have verified that, at specific ratios, this growth regulator significantly increases peanut yield. Under intercropping conditions of sugarcane and peanuts, the yield increase is even more significant, and the applicable concentration is wider. This growth regulator originates from the microbial fermentation process of plant materials, making it potentially environmentally friendly. Furthermore, the combination of different regulators exhibits a synergistic effect, providing a new approach for developing specialized agricultural biological agents. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the sugarcane-peanut intercropping experiment of this application.

[0018] Figure 2 is a schematic diagram showing the growth of peanut plants in the sugarcane-peanut intercropping system after the special growth regulator is added; A is a peanut plant applied with the special growth regulator, and B is a blank control plant without any growth regulator applied.

[0019] Biological material deposit information.

[0020] The information of the strain deposited in the present application is: streptococcus alactolyticus GX-2, its taxonomic designation is: streptococcus alactolyticus, its Chinese taxonomic designation is: non-lactolytic streptococcus, and its deposit number is GDMCC NO: 65508; the strain is deposited in the Guangdong Microbial Culture Collection Center, address: Institute of Microbiology, Guangdong Academy of Sciences, 5th Floor, Building 59, Compound No. 100, Xianlie Middle Road, Guangzhou, and the deposit date is November 19, 2024.

[0021] The information of the strain deposited in the present application is: streptococcus alactolyticus GX-7, its taxonomic designation is: streptococcus alactolyticus, its Chinese taxonomic designation is: non-lactolytic streptococcus, and its deposit number is GDMCC NO: 65509; the strain is deposited in the Guangdong Microbial Culture Collection Center, address: Institute of Microbiology, Guangdong Academy of Sciences, 5th Floor, Building 59, Compound No. 100, Xianlie Middle Road, Guangzhou, and the deposit date is November 19, 2024. Detailed Description of Embodiments

[0022] All features disclosed in this specification, or all steps in the disclosed methods or processes, can be combined in any manner, except for mutually exclusive features and / or steps.

[0023] Any feature disclosed in this specification, unless specifically stated otherwise, is merely an example of a series of equivalent or similar features.

[0024] Example 1

[0025] This example studies the effect of fermentation products of streptococcus alactolyticus on the growth of peanut seedlings.

[0026] Strains: streptococcus alactolyticus GX-2 and streptococcus alactolyticus GX-7.

[0027] Fermentation substrate: In our previous feed, we found that mulberry leaves, sugarcane leaves and peanut vines produce indole compounds when fermented with lactic acid bacteria. Therefore, we selected mulberry leaves, sugarcane leaves and peanut vines for regulator research. Specifically, fresh mulberry leaves, sugarcane leaves and peanut vines were mixed with water at a solid-liquid ratio of 3:1 and then pulped to obtain the substrate.

[0028] Fermentation method: After activating the above strains GX-2 and GX-7, inoculate them into the above substrates at an inoculation rate of 5%. After fermentation for 30 days, filter the solution and concentrate it to 1 / 10 of the original solution to obtain the corresponding microbial growth regulator.

[0029] Peanut seeds were soaked in sterile water for 12 hours for disinfection, washed, and then germinated. Seeds showing signs of germination were selected and sown in a sterilized substrate for seedling cultivation. When the seedlings had 2-3 true leaves, healthy and uniform seedlings were transplanted into a sterilized mixture of calcareous soil and quartz sand in a 3:2 ratio. Seedlings of the same height were grouped into 7 groups (3 levels per group). Following the grouping in Table 1, a fermented microbial growth regulator (prepared as a 10g / L solution) was sprayed onto the potted plants every 15 days until the leaves were moist. The control group was sprayed with the same amount of water. Harvesting was carried out after 60 days of cultivation. The light chamber conditions were: 25℃ for 17 hours of light and 17℃ for 7 hours of darkness. The aboveground biomass, plant height, and root length of each plant were measured, and the results are shown in Table 1.

[0030]

[0031] Note: Different lowercase letters in the table indicate significant differences in the data of the same column (p<0.05), and the same letters indicate no significant differences in the data of the same column (p>0.05). The same applies to the following table.

[0032] As shown in Table 1, the peanut biomass from highest to lowest was: GX-2-sugarcane leaf microbial growth regulator > GX-7-peanut vine microbial growth regulator > GX-2-mulberry leaf microbial growth regulator > GX-2-peanut vine microbial growth regulator > GX-7-sugarcane leaf microbial growth regulator > control group (CK) > GX-7-mulberry leaf microbial growth regulator. There were no significant differences among the groups (p>0.05).

[0033] In terms of plant height: GX-7-peanut vine microbial growth regulator > GX-2-mulberry leaf microbial growth regulator > GX-2-sugarcane leaf microbial growth regulator > control group (CK) > GX-7-sugarcane leaf microbial growth regulator > GX-2-peanut vine microbial growth regulator > GX-7-mulberry leaf microbial growth regulator, and the differences among the groups were not significant (p>0.05).

[0034] In terms of root length: GX-2-sugarcane leaf microbial growth regulator > GX-2-mulberry leaf microbial growth regulator > GX-7-mulberry leaf microbial growth regulator > GX-7-sugarcane leaf microbial growth regulator > GX-7-peanut vine microbial growth regulator > GX-2-peanut vine microbial growth regulator > control group (CK), and the differences among the groups were not significant (p>0.05).

[0035] This indicates that the microbial growth regulators produced by the fermentation of GX-2 and GX-7 strains have varying degrees of promoting effect on the growth of peanut seedlings, but the differences are not significant. This suggests that these growth regulators have certain research value. Considering the various indicators, we selected GX-2-mulberry leaf microbial growth regulator, GX-2-sugarcane leaf microbial growth regulator, and GX-7-peanut vine microbial growth regulator, which have promoting effects on peanut seedling biomass, plant height, and root length, for further field experiments.

[0036] Example 2

[0037] This embodiment studies the effects of the microbial growth regulator prepared in Example 1 on peanut field cultivation, as detailed below.

[0038] (1) Randomized block design, 3 replicates.

[0039] (2) Planting specifications: single seed sowing, plant spacing 12.5cm.

[0040] (3) Variety: Osmanthus 376.

[0041] (4) Peanut-specific growth regulator treatment.

[0042] Peanut seedlings 30 days after planting were sprayed with either GX-2-mulberry leaf microbial growth regulator, GX-2-sugarcane leaf microbial growth regulator, or GX-7-peanut vine microbial growth regulator as the experimental group. Spraying was sufficient to moisten the leaves. The control group was sprayed with the same amount of water, once every 15 days. Routine field management was maintained, with no additional fertilization. Harvest was conducted 120 days after sowing. Plant height, dry weight, and yield of peanuts at harvest were measured.

[0043]

[0044] As shown in Table 2, in terms of plant height and dry weight, GX-2-mulberry leaf microbial growth regulator, GX-2-sugarcane leaf microbial growth regulator, and GX-7-peanut vine microbial growth regulator were all higher than the control group. Among them, the plant height and dry weight of GX-7-peanut vine microbial growth regulator were significantly different from the control group (p<0.05), the dry weight of GX-2-sugarcane leaf microbial growth regulator was significantly different from the control group (p<0.05), but the plant height was not significantly different (p>0.05), and the plant height and dry weight of GX-2-mulberry leaf microbial growth regulator were not significantly different from the control group (p>0.05).

[0045] The yields of GX-2-mulberry leaf microbial growth regulator, GX-2-sugarcane leaf microbial growth regulator, and GX-7-peanut vine microbial growth regulator were basically the same as those of the control group (CK). The yield of GX-2-mulberry leaf microbial growth regulator was even slightly lower than that of the control group (CK), but the difference was not significant (p>0.05). This indicates that microbial growth regulators can promote peanut plant growth to some extent, but they do not significantly increase peanut yield, suggesting that microbial growth regulators cannot improve peanut yield.

[0046] To increase peanut yield, we considered using microbial growth regulators combined with dwarfing agents to control excessive vegetative growth and shift the focus from above-ground vegetative growth to the reproductive growth of the underground pods, thereby increasing yield. We selected two microbial growth regulators that improve peanut yield, plant height, and dry weight: GX-2-sugarcane leaf microbial growth regulator and GX-7-peanut vine microbial growth regulator, combined with dwarfing agents: brassinolide and jasmonic acid, to prepare a special growth regulator. The specific application method is as follows: Spray the microbial growth regulator starting 30 days after peanut sowing, marking this application as the first time. The microbial growth regulator is applied according to... The experimental formulation consisted of a mixture of GX-2-sugarcane leaf microbial growth regulator and GX-7-peanut vine microbial growth regulator. This mixture was sprayed twice, with a 15-day interval between each application. The third application (60 days after planting) involved spraying a special growth regulator containing a dwarfing agent. This special growth regulator was composed of GX-2-sugarcane leaf microbial growth regulator, GX-7-peanut vine microbial growth regulator, and a dwarfing agent. Subsequent applications of the special growth regulator were made every 15 days. Standard field management was followed, with no additional fertilization. Peanuts were harvested 120 days after sowing. Plant height, dry weight, and yield were measured at harvest, and the results are shown in Table 3.

[0047]

[0048] As shown in Table 3, the plant height of experimental group 1 was significantly higher than that of experimental groups 2-9 (p<0.05). Simultaneously, the plant height of experimental group 1 was also higher than that of experimental group 10, but the difference was not significant. This may be because the content of the dwarfing agents brassinolide and jasmonic acid in experimental group 1 was lower, failing to achieve a complete and effective control of excessive growth; it may also be due to the presence of a ratio threshold or antagonistic effect of the dwarfing agents. At other concentration ratios, the dedicated growth regulator effectively controlled peanut plant height, preventing excessive growth and achieving effective control of excessive growth.

[0049] The dry weights from highest to lowest were: Group 4 > Group 6 > Group 3 > Group 2 > Group 5 > Group 7 > Group 1 > Group 9 > Group 8 > Group 10. This indicates that the dry weights of Groups 1-7 were significantly higher than those of Group 10. This also shows that the growth dwarfing agent can promote the accumulation of photosynthetic products after controlling the growth, resulting in stronger peanut plants, which is consistent with the characteristics of the growth dwarfing agent.

[0050] The yields, from highest to lowest, were: Group 4 > Group 7 > Group 3 > Group 2 > Group 8 > Group 1 > Group 9 > Group 5 > Group 10 > Group 6. The results showed that although the yields of Groups 1-5 and Groups 7-9 were lower than the control group (Group 10), except for Group 4, the differences between the other groups and the control group were not significant (p>0.05). This indicates that adding a dwarfing agent to control excessive growth can increase peanut yield. The mixture of dwarfing agent and microbial growth regulator improved the problems of excessive vegetative growth and failure to produce fruit, thus increasing peanut yield. In Group 6, although the dwarfing agent was fully added, the yield did not increase, indicating that there is a ratio threshold or antagonistic effect of the dwarfing agent.

[0051] Based on the above experimental results and with yield as the guiding principle, we selected experimental group 4 as the optimal result. We concluded that the best application method for the specialized growth regulator in peanut field planting is as follows: Spray the microbial growth regulator starting 30 days after peanut sowing, marking this spray as the first application. The microbial growth regulator consists of 2 g / L of GX-2-sugarcane leaf microbial growth regulator and 2 g / L of GX-7-peanut vine microbial growth regulator. Spray again after 15 days, for a total of two applications. For the third application (60 days after planting), spray the specialized growth regulator with added dwarfing agents. This specialized growth regulator consists of 2 g / L of GX-2-sugarcane leaf microbial growth regulator, 2 g / L of GX-7-peanut vine microbial growth regulator, 0.2 g / L of brassinolide, and 0.3 g / L of jasmonic acid. Spray the specialized growth regulator every 15 days thereafter until harvesting peanuts on the 120th day after planting.

[0052] Example 3

[0053] This embodiment studies the effects of microbial growth regulators on the growth of sugarcane-peanut intercropping. Referring to the wide-narrow row intercropping pattern in the previously published paper "Research on Peanut Variety Screening and High-Efficiency Cultivation Model of Sugarcane + Peanut Intercropping," the specific details are as follows:

[0054] (1) Randomized block design, 3 replicates.

[0055] (2) Planting pattern: Wide-row cluster sugarcane + peanut intercropping pattern. To suit mechanized production, the wide row spacing of sugarcane is set at 2.4 and 2.6 m, and the narrow row spacing is set at 1.2 and 1.3 m, that is, 2.4 m equal row spacing, 1.2 m + 2.4 m wide and narrow row spacing, and 1.3 m + 2.6 m wide and narrow row spacing. The hole spacing is 0.55 m, and each hole has 8 effective stems, which is equivalent to 67,500 effective stems / hm. 2 Four rows of peanuts are planted between sugarcane rows, using the same high-yield cultivation technique of ridging, double-row single-seed precision sowing as in monoculture. Peanuts are sown individually with a plant spacing of 12.5cm. A diagram of intercropping can be found here. Figure 1 .

[0056] (3) Variety: Osmanthus 376.

[0057] (4) Spraying of peanut-specific growth regulators for sugarcane intercropping: GX-2-mulberry leaf microbial growth regulator, GX-2-sugarcane leaf microbial growth regulator, and GX-7-peanut vine microbial growth regulator were selected and sprayed on peanut plants 30 days after planting. Spraying was sufficient to moisten the leaves. This group served as the experimental group. The control group (CK) was sprayed with the same amount of water. Spraying was carried out every 15 days, with routine field management. Harvest was carried out 120 days after sowing. The plant height, dry weight, and yield of peanuts at the harvest period were tested.

[0058]

[0059] As shown in Table 4, after intercropping sugarcane and peanuts, in terms of plant height, GX-7-peanut vine microbial growth regulator > GX-2-sugarcane leaf microbial growth regulator > control group > GX-2-mulberry leaf microbial growth regulator. The plant height of the experimental groups with GX-7-peanut vine microbial growth regulator and GX-2-sugarcane leaf microbial growth regulator was significantly higher than that with GX-2-mulberry leaf microbial growth regulator (p<0.05). There was no significant difference compared with the control group (p>0.05). This indicates that GX-7-peanut vine microbial growth regulator and GX-2-sugarcane leaf microbial growth regulator can significantly increase the plant height of peanut plants under the intercropping pattern compared with the GX-2-mulberry leaf microbial growth regulator group.

[0060] In terms of dry weight, GX-2-sugarcane leaf microbial growth regulator > GX-7-peanut vine microbial growth regulator > control group > GX-2-mulberry leaf microbial growth regulator. However, the differences in dry weight among the groups were not significant (p>0.05), indicating that under sugarcane-peanut intercropping conditions, none of the microbial growth regulators had a significant growth-promoting effect on peanut crops. Combined with plant height data, this suggests that under this model, peanut plants exhibit excessive vegetative growth and are not robust; therefore, although there are large differences in plant height, the differences in dry weight are not significant.

[0061] In terms of yield, GX-7-peanut vine microbial growth regulator > GX-2-sugarcane leaf microbial growth regulator > control group > GX-2-mulberry leaf microbial growth regulator. However, there was no significant difference in dry weight among the groups (p>0.05). This indicates that under sugarcane-peanut intercropping conditions, none of the microbial growth regulators promoted peanut yield. Combined with dry weight and plant height data, we further confirmed that microbial growth regulators do not make plants robust or improve peanut seed setting ability.

[0062] The growth-promoting effect of peanut-sugarcane intercropping also differs from that of monoculture. This indicates that the soil microbial community may change after intercropping, and the growth environment, such as light and temperature, will change due to intercropping. This leads to different effects of growth regulators under intercropping and monoculture conditions. Therefore, to prevent excessive peanut vegetative growth leading to seedling growth without fruiting, we considered mixing the two microbial growth regulators mentioned above with dwarfing agents: brassinolide and jasmonic acid, to prepare a special growth regulator. The preparation scheme of the special growth regulator is based on orthogonal array design. After mixing according to the mass concentration in Table 5, sugarcane-peanut intercropping is carried out. The specific spraying method is as follows: spray the microbial growth regulator starting 30 days after peanut sowing. The growth regulator was applied, and this application was marked as the first application. The microbial growth regulator was prepared according to the experimental group ratio by mixing GX-2-sugarcane leaf microbial growth regulator and GX-7-peanut vine microbial growth regulator. After that, it was sprayed again after 15 days, for a total of 2 applications. The third application (60 days after planting) was a special growth regulator with added dwarfing agent. The special growth regulator was composed of GX-2-sugarcane leaf microbial growth regulator, GX-7-peanut vine microbial growth regulator and dwarfing agent. After that, the special growth regulator was sprayed every 15 days. Conventional field management was carried out without additional fertilization. The peanuts were harvested 120 days after sowing. The plant height, dry weight and yield of peanuts at harvest were measured. The results are shown in Table 5.

[0063]

[0064] Table 5 shows that, in terms of plant height, the order from highest to lowest is: Experimental Group 1 > Experimental Group 10 > Experimental Group 4 > Experimental Group 8 > Experimental Group 9 > Experimental Group 2 > Experimental Group 6 > Experimental Group 5 > Experimental Group 3 > Experimental Group 7. The plant height of Experimental Group 1 was not significantly different from other experimental groups and the control group (Experimental Group 10) without added dwarfing agents. This may be because the content of brassinolide and jasmonic acid in Experimental Group 1 was relatively low, failing to achieve a complete and effective control of excessive growth; it may also be due to a ratio threshold or antagonistic effect of the dwarfing agents. At other concentration ratios, the dedicated growth regulator effectively controlled peanut plant height, preventing excessive growth and achieving effective control of excessive growth.

[0065] In terms of dry weight, the order from highest to lowest is: Group 4 > Group 2 > Group 7 > Group 1 > Group 8 > Group 5 > Group 9 > Group 3 > Group 6 > Group 10. This indicates that the growth dwarfing agent can promote the accumulation of photosynthetic products, and the peanut plants grow stronger, which is consistent with the characteristics of the growth dwarfing agent.

[0066] In terms of yield, the order from highest to lowest was: Experimental Group 2 > Experimental Group 7 > Experimental Group 4 > Experimental Group 8 > Experimental Group 9 > Experimental Group 3 > Experimental Group 1 > Experimental Group 6 > Experimental Group 10 > Experimental Group 5. The yields of Experimental Groups 2, 7, 4, and 8 were significantly different from those of Experimental Group 10, while the yields of Experimental Groups 1, 3, 6, and 9 were not significantly different from those of Experimental Group 10. This indicates that adding a dwarfing agent to control excessive growth can increase peanut yield. The combination of the dwarfing agent and the microbial growth regulator improved the problems of excessive vegetative growth and failure to produce fruit, thus increasing peanut yield. Although the dwarfing agent was fully added to Experimental Group 5, the yield did not increase, indicating that there is a ratio threshold or antagonistic effect of the dwarfing agent.

[0067] Therefore, considering all factors, we believe that the growth regulator can achieve the effect of increasing yield under the experimental conditions of experimental groups 2, 4, 7, and 8. The optimal experimental conditions are those of experimental group 2, i.e., in the sugarcane-peanut intercropping system, where the special growth regulator composed of 1 g / L-3 g / L of GX-2-sugarcane leaf microbial growth regulator, 2 g / L-4 g / L of GX-7-peanut vine microbial growth regulator, 0.1 g / L-0.3 g / L of brassinolide, and 0.2 g / L-0.3 g / L of jasmonic acid can significantly promote peanut yield growth. The specific growth regulator, composed of 1 g / L GX-2-sugarcane leaf microbial growth regulator, 4 g / L GX-7-peanut vine microbial growth regulator, 0.2 g / L brassinolide, and 0.2 g / L jasmonic acid, showed the highest effect on increasing peanut yield. Therefore, we selected the best-performing experimental group, experimental group 2, and conducted a separate verification experiment with the control group. The final results are as follows: Figure 2 As shown, Figure 2In Figure A, the experimental group treated with the special growth regulator was used, and in Figure B, the control group was not treated with the regulator. As can be seen from the figure, the height of the two plants is not much different, but the fruits of plant A are larger and the pods are fuller than those of plant B. This shows that the method of this application, which involves first applying a microbial growth regulator and then using a special growth regulator with added dwarfing agent to control the growth, can increase the yield of peanuts in the sugarcane-peanut intercropping system.

[0068] We determined the optimal application method for specialized growth regulators in peanut-sugarcane field planting as follows: Spray the microbial growth regulator starting 30 days after peanut sowing, marking this spray as the first application. The microbial growth regulator consists of 1 g / L of GX-2-sugarcane leaf microbial growth regulator and 4 g / L of GX-7-peanut vine microbial growth regulator. Repeat the application after 15 days, for a total of two applications. The third application (60 days after planting) is then performed. d) Begin spraying a special growth regulator with added dwarfing agent. The special growth regulator consists of 1 g / L of GX-2-sugarcane leaf microbial growth regulator, 4 g / L of GX-7-peanut vine microbial growth regulator, 0.2 g / L of brassinolide, and 0.2 g / L of jasmonic acid. Spray the special growth regulator every 15 days thereafter. Harvest peanuts on the 120th day after planting. The sugarcane-peanut intercropping method adopts a wide-row clump-type sugarcane + peanut compound cultivation model.

[0069] In summary, experiments have verified that one or a mixture of two of the GX-2-sugarcane leaf microbial growth regulator and GX-7-peanut vine microbial growth regulators in this application can promote the growth of peanut plants. However, these microbial growth regulators have not improved peanut yield. Therefore, we have formulated a dwarfing agent to prepare a growth regulator that can improve peanut yield. The special growth regulator in this application is composed of GX-2-sugarcane leaf microbial growth regulator, GX-7-peanut vine microbial growth regulator, brassinolide, and jasmonic acid. Under specific ratios, this growth regulator can significantly control peanut plant height and increase peanut pod yield, and it is also effective under sugarcane-peanut intercropping conditions. This invention broadens the application of strains GX-2 and GX-7 in promoting plant growth and provides a new microbial growth-promoting technology solution.

[0070] The above-described embodiments are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention.

Claims

1. A peanut-specific growth regulator, characterized in that, The peanut-specific growth regulator is composed of 1 g / L-3 g / L of GX-2-sugarcane leaf microbial growth regulator, 2 g / L-4 g / L of GX-7-peanut vine microbial growth regulator, 0.1 g / L-0.3 g / L of brassinolide, and 0.2 g / L-0.3 g / L of jasmonic acid. The GX-2 sugarcane leaf microbial growth regulator is produced by non-lactolytic streptococci (… streptococcus alactolyticus The strain GX-2 was inoculated into sugarcane leaf slurry at a 5% inoculation rate, fermented for 30 days, filtered, and the filtrate was concentrated to 1 / 10 of the original liquid to obtain the product. The GX-7 peanut vine microbial growth regulator is produced by non-lactolytic streptococci (… streptococcus alactolyticus The strain GX-7 was inoculated into the slurry of peanut vines at a rate of 5%, fermented for 30 days, filtered, and the filtrate was concentrated to 1 / 10 of the original solution to obtain the product. The non-lactolytic streptococci ( streptococcus alactolyticus The accession number of strain GX-2 is GDMCCNO: 65508, the depositary institution is Guangdong Provincial Center for Microbial Culture Collection, the depositary address is: Institute of Microbiology, Guangdong Academy of Sciences, 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Guangdong Province, and the deposit date is November 19, 2024. The accession number of the Streptococcus alactolyticus strain GX-7 is GDMCCNO: 65509, the depositary institution is Guangdong Provincial Center for Microbial Culture Collection, the depositary address is: Institute of Microbiology, Guangdong Academy of Sciences, 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Guangdong Province, and the deposit date is November 19, 2024.

2. The peanut-specific growth regulator according to claim 1, characterized in that, The peanut-specific growth regulator consists of 2 g / L of GX-2-sugarcane leaf microbial growth regulator, 2 g / L of GX-7-peanut vine microbial growth regulator, 0.2 g / L of brassinolide, and 0.3 g / L of jasmonic acid.

3. The application of the peanut-specific growth regulator as described in claim 1 in sugarcane-peanut intercropping, characterized in that, The method for intercropping sugarcane and peanuts is as follows: After intercropping sugarcane stalks and peanut seeds, cultivate for 30 days. On the 30th day, spray with a microbial growth regulator consisting of GX-2-sugarcane leaf microbial growth regulator and GX-7-peanut vine microbial growth regulator, and mark this spraying as the first time. Spray again after 15 days, for a total of 2 sprays of microbial growth regulator. On the third time, spray with the peanut-specific growth regulator. After that, spray the peanut-specific growth regulator once every 15 days, with conventional field management, no additional fertilization, and harvest 120 days after sowing.

4. The application according to claim 3, characterized in that, The peanut-specific growth regulator consists of 1 g / L of GX-2-sugarcane leaf microbial growth regulator, 4 g / L of GX-7-peanut vine microbial growth regulator, 0.2 g / L of brassinolide, and 0.2 g / L of jasmonic acid.

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