A whitening soothing composition and uses thereof

Abstract

The present application relates to a kind of whitening soothing composition and its application, including the following weight parts of component: tianshan saussurea involucrata cell culture, hydrolyzed silk fibroin and jasmine flower extract;The mass ratio of tianshan saussurea involucrata cell culture, hydrolyzed silk fibroin and jasmine flower extract is (1-3) :(0.3-1.5) :(2-8);Wherein, the jasmine flower extract is obtained using two-stage gradient oxygen fermentation process, the whitening soothing composition of the present application is compounded by tianshan saussurea involucrata cell culture, hydrolyzed silk fibroin and fermented jasmine flower extract, realizes the multiple effects of inhibiting melanin production, antioxidant lightening, anti-inflammatory soothing, skin barrier repair synergy, overcomes the defects such as traditional whitening product easy irritation, soothing product weak whitening effect, low utilization rate of plant raw materials, finally obtains a kind of whitening effect significant and mild non-irritating composition, can be widely used in cosmetic field.

Description

Technical Field

[0001] This invention relates to the technical field of cosmetics, specifically to a whitening and soothing composition and its application. Background Technology

[0002] A long-standing problem in the whitening ingredient industry is that potent effects often mean irritation, while gentleness is often equated with "concept outweighing practicality." While chemically synthesized whitening ingredients (such as niacinamide, kojic acid, and tranexamic acid) show faster results, some are highly irritating, have low sensitivity to sensitive skin, and may even cause redness, stinging, and other discomfort. Many existing whitening products target only a single step or signaling pathway in melanin production, resulting in limited effectiveness and difficulty in achieving significant whitening effects. They often employ single chemically synthesized whitening ingredients (such as adding only kojic acid or only niacinamide), lacking multi-pathway synergistic design. Single-pathway whitening strategies are prone to reaching performance plateaus or developing drug resistance. Traditional plant extraction methods (such as solvent extraction) often only extract large molecules, resulting in poor transdermal absorption.

[0003] Many whitening products on the market prioritize whitening effects while neglecting soothing and repairing functions, making them unsuitable for people with sensitive skin. While whitening ingredients inhibit melanin production, they may also create additional irritation and burden on the skin, and existing products lack the complementary design of effective soothing and barrier repair mechanisms. Summary of the Invention

[0004] The purpose of this invention is to overcome the problems of existing whitening and soothing compositions, which mostly use simple compounding of whitening active ingredients and soothing ingredients, resulting in high irritation of whitening ingredients, low retention of plant raw material activity, insufficient extraction, single efficacy, and poor synergy. This invention provides a whitening and soothing composition and its application.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a whitening and soothing composition comprising the following components in parts by weight: Tian Shan snow lotus cell culture, hydrolyzed silk protein and jasmine flower extract; the mass ratio of Tian Shan snow lotus cell culture, hydrolyzed silk protein and jasmine flower extract is (1-3):(0.3-1.5):(2-8); The preparation method of the jasmine flower extract includes the following steps: S1. Mix dried jasmine powder with 10-15 times its weight of deionized water, sterilize, and cool to obtain the first fermentation substrate; S2. Inoculate the first fermentation substrate with Staphylococcus aureus culture for fermentation. After fermentation, sterilize and cool to obtain the second fermentation substrate. S3. Inoculate the second fermentation substrate with Mycosaccharomyces cerevisiae and Lactococcus lactis culture, and carry out fermentation. After fermentation, sterilize, cool, centrifuge and filter to obtain fermentation broth and fermentation residue. S4. Add the fermentation residue obtained in step S3 to 6-10 times its weight of deionized water for ultrasonic extraction, centrifuge, and collect the supernatant. S5. Mix the fermentation broth obtained in step S3 and the supernatant collected in step S4, and freeze-dry to obtain the jasmine flower extract.

[0006] The functions of the components in the whitening and soothing composition of the present invention are as follows: The active ingredients in Tian Shan snow lotus cell culture mainly include polysaccharides, flavonoids, alkaloids, sterols, and volatile oils. These active ingredients endow it with anti-inflammatory, anti-radiation, free radical scavenging, antibacterial, and antiviral biological functions. Compared with the content of active ingredients in natural plant extracts, the total flavonoid and total protein content of snow lotus culture is significantly increased, further enhancing the whitening and soothing effects of the composition.

[0007] Hydrolyzed silk protein is rich in various amino acids and small-molecule proteins, which are easily absorbed by the skin, providing the nutrients needed for whitening and further promoting skin tone improvement. Hydrolyzed silk protein can also promote the proliferation and migration of fibroblasts and increase the content of barrier-related proteins, thereby helping to repair damaged skin barriers.

[0008] The jasmine flower extract of this invention is obtained using a two-stage gradient aerobic fermentation process: In the first stage, under strong oxygen conditions, *Gastrodia elata* efficiently produces gluconic acid, gently exfoliating dead skin cells and inhibiting tyrosinase, achieving whitening, brightening, anti-oxidation, and yellowing removal; in the second stage, under micro-oxygen conditions, *Mycospermum jasminoides* and *Lactococcus lactis* synergistically metabolize to produce lactic acid, small molecule peptides, extracellular polysaccharides, sterols, etc., exerting anti-inflammatory, soothing, barrier-repairing, and irritation-reducing effects, and further releasing the active ingredients of jasmine. The fermentation residue is ultrasonically broken down to extract bound polyphenols, flavonoids, and polysaccharides, which, combined with the fermentation broth containing soluble probiotic metabolites, significantly improves the utilization rate of raw materials.

[0009] Preferably, the mass ratio of Tian Shan snow lotus cell culture, hydrolyzed silk protein and jasmine flower extract is (1.5-2):(0.5-1):(3-5).

[0010] Preferably, the preparation method of the jasmine flower powder includes the following steps: washing the jasmine flowers, first performing infrared drying, and then freeze-drying; wherein the parameters for infrared drying are: vacuum degree of (-0.06)-(-0.09) MPa, irradiation distance of 100-150 mm, power density of 1-2 W / g, infrared wavelength of 3-5 μm, temperature of 35-45℃, and time of 30-60 min. This invention pre-treats jasmine flowers by combining low-temperature infrared drying with vacuum freeze-drying, making the cells loose and porous, maximizing the retention of active ingredients such as flavonoids, polyphenols, and polysaccharides, and avoiding oxidative browning.

[0011] Preferably, in step S1 of preparing jasmine flower extract, the sterilization temperature is 115-121℃ and the time is 15-40 min.

[0012] Preferably, in step S2 of preparing the jasmine flower extract, the inoculum size of the *Staphylococcus aureus* suspension is 4-7% v / v, and the viable count of the *Staphylococcus aureus* suspension is 10-1. 7 -10 9 The fermentation temperature was 25-27℃, the oxygen supply was 15-20%, and the time was 24-72h; the sterilization temperature was 80-105℃ and the time was 20-40min.

[0013] Preferably, in step S3 of preparing the jasmine flower extract, the total inoculum amount of the *Saccharomyces cerevisiae* and *Lactococcus lactis* cultures is 3-5% v / v, and the total viable count is 10. 7 -10 9 CFU / mL, the live count ratio of Mycosaccharomyces cerevisiae and Lactococcus lactis is 1:(0.5-1.5); the fermentation temperature is 26-32℃, the oxygen supply is 0.1-5%, and the time is 24-48h; the sterilization temperature is 80-105℃ and the time is 20-40min.

[0014] Preferably, in step S4 of preparing jasmine flower extract, the ultrasonic extraction temperature is 30-40℃, the power is 100-300W, and the time is 20-40min.

[0015] In a second aspect, the present invention provides the application of the whitening and soothing composition of the first aspect in the preparation of cosmetics.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: The whitening and soothing composition of this invention, through the combination of Tian Shan snow lotus cell culture, hydrolyzed silk protein and fermented jasmine flower extract, achieves multiple synergistic effects of inhibiting melanin production, anti-oxidation and brightening, anti-inflammatory and soothing, and repairing the skin barrier. It overcomes the shortcomings of traditional whitening products such as easy irritation, weak whitening effect of soothing products, and low utilization rate of plant raw materials. The final result is a composition with significant whitening effect and gentle and non-irritating properties, which can be widely used in the cosmetics field. Detailed Implementation

[0017] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0018] The sources of the raw materials used in the following examples and comparative examples are as follows: Tianshan Snow Lotus Cell Culture: Manufacturer: Chongqing Ansaibo New Material Technology Co., Ltd.; Product Name: Tianshan Snow Lotus Pure Essence; Tianshan Snow Lotus Cell Culture Content: 30wt% Hydrolyzed silk protein: Manufacturer is SilkyMed Biotechnology (Zhejiang) Co., Ltd., product name is Silky Inner. ® sfp01; Staphylococcus aureus: Manufacturer: Wuhan Gray Algae Biotechnology Co., Ltd., Product No.: HZB197780; Hypnotypus: purchased from China General Microbiological Culture Collection Center, accession number CGMCC No. 29720; Lactococcus lactis: purchased from the American Center for Type Culture Collection, catalog number ATCC13675; Unless otherwise specified, all other materials, reagents, etc. used in the examples and comparative examples are commercially available.

[0019] Examples 1-5 and Comparative Examples 1-5 The components and their weight parts of the whitening and soothing compositions of Examples 1-5 and Comparative Examples 1-5 are shown in Table 1. The total weight of each composition is the same.

[0020] Table 1

[0021] The preparation methods of the jasmine flower extracts in Examples 1-5 and Comparative Examples 1-5 include the following steps: S1. Mix jasmine flower powder with 12 times its mass of deionized water, sterilize, and cool to obtain the first fermentation substrate; wherein, the preparation method of the jasmine flower powder includes the following steps: washing jasmine flowers, first performing infrared drying, and then freeze-drying; wherein, the parameters for infrared drying are: vacuum degree of -0.08MPa, irradiation distance of 120mm, power density of 1.5W / g, infrared wavelength of 4μm, temperature of 40℃, and time of 50min; the sterilization temperature is 118℃ and the time is 20min; S2. Inoculate the first fermentation substrate with *Gastrodia elata* bacterial solution for fermentation. After fermentation, sterilize and cool to obtain the second fermentation substrate. The inoculum size of the *Gastrodia elata* bacterial solution is 5% v / v, and the viable count of the *Gastrodia elata* bacterial solution is 3 × 10⁻⁶. 8 The fermentation temperature was 26℃, the oxygen supply was 18%, and the time was 36h; the sterilization temperature was 90℃ and the time was 30min. S3. Inoculate the second fermentation substrate with Mycosaccharomyces cerevisiae and Lactococcus lactis cultures for fermentation. After fermentation, sterilize, cool, centrifuge, and filter to obtain fermentation broth and fermentation residue. The total inoculum size of Mycosaccharomyces cerevisiae and Lactococcus lactis cultures is 4% v / v, and the total viable count is 3.5 × 10⁻⁶. 8 CFU / mL, viable count ratio of Mycosaccharomyces cerevisiae to Lactococcus lactis was 1:1.3; fermentation temperature was 30℃, oxygen supply was 1%, and time was 36h; sterilization temperature was 90℃ and time was 25min. S4. Add the fermentation residue obtained in step S3 to 7 times its mass of deionized water for ultrasonic extraction, centrifuge, and collect the supernatant; wherein, the ultrasonic extraction temperature is 35℃, the power is 200W, and the time is 30min. S5. Mix the fermentation broth obtained in step S3 and the supernatant collected in step S4, and freeze-dry to obtain the jasmine flower extract.

[0022] Comparative Example 6 The only difference between Comparative Example 6 and Example 1 is that the jasmine flower extract is not fermented, and the specific preparation method includes the following steps: S1. Add jasmine flower powder to 7 times its weight of deionized water for ultrasonic extraction, centrifuge, and collect the supernatant; wherein, the preparation method of the jasmine flower powder includes the following steps: washing jasmine flowers, first performing infrared drying, and then freeze-drying; wherein, the parameters for infrared drying are: vacuum degree of -0.08MPa, irradiation distance of 120mm, power density of 1.5W / g, infrared wavelength of 4μm, temperature of 40℃, and time of 50min; the ultrasonic extraction temperature is 35℃, power is 200W, and time is 30min.

[0023] Comparative Example 7 The only difference between Comparative Example 7 and Example 1 is that the jasmine flower extract does not undergo fermentation with Staphylococcus aureus in Japan. The specific preparation steps are as follows: S1. Mix jasmine flower powder with 12 times its mass of deionized water, sterilize, and cool to obtain the first fermentation substrate; wherein, the preparation method of the jasmine flower powder includes the following steps: washing jasmine flowers, first performing infrared drying, and then freeze-drying; wherein, the parameters for infrared drying are: vacuum degree of -0.08MPa, irradiation distance of 120mm, power density of 1.5W / g, infrared wavelength of 4μm, temperature of 40℃, and time of 50min; the sterilization temperature is 118℃ and the time is 20min; S2. Inoculate the first fermentation substrate with Mycosaccharomyces cerevisiae and Lactococcus lactis cultures for fermentation. After fermentation, sterilize, cool, centrifuge, and filter to obtain fermentation broth and fermentation residue. The total inoculum size of Mycosaccharomyces cerevisiae and Lactococcus lactis cultures is 4% v / v, and the total viable count is 3.5 × 10⁻⁶. 8 CFU / mL, viable count ratio of Mycosaccharomyces cerevisiae to Lactococcus lactis was 1:1.3; fermentation temperature was 30℃, oxygen supply was 1%, and time was 36h; sterilization temperature was 90℃ and time was 25min. S3. Add the fermentation residue obtained in step S2 to 7 times its mass of deionized water for ultrasonic extraction, centrifuge, and collect the supernatant; wherein, the ultrasonic extraction temperature is 35℃, the power is 200W, and the time is 30min. S4. Mix the fermentation broth obtained in step S2 and the supernatant collected in step S3, and freeze-dry to obtain the jasmine flower extract.

[0024] Comparative Example 8 The only difference between Comparative Example 8 and Example 1 is that the jasmine flower extract does not undergo fermentation with Mycosaccharomyces cerevisiae in its fermentation step. The specific preparation steps are as follows: S1. Mix jasmine flower powder with 12 times its mass of deionized water, sterilize, and cool to obtain the first fermentation substrate; wherein, the preparation method of the jasmine flower powder includes the following steps: washing jasmine flowers, first performing infrared drying, and then freeze-drying; wherein, the parameters for infrared drying are: vacuum degree of -0.08MPa, irradiation distance of 120mm, power density of 1.5W / g, infrared wavelength of 4μm, temperature of 40℃, and time of 50min; the sterilization temperature is 118℃ and the time is 20min; S2. Inoculate the first fermentation substrate with *Gastrodia elata* bacterial solution for fermentation. After fermentation, sterilize and cool to obtain the second fermentation substrate. The inoculum size of the *Gastrodia elata* bacterial solution is 5% v / v, and the viable count of the *Gastrodia elata* bacterial solution is 3 × 10⁻⁶. 8 The fermentation temperature was 26℃, the oxygen supply was 18%, and the time was 36h; the sterilization temperature was 90℃ and the time was 30min. S3. Inoculate the second fermentation substrate with Lactococcus lactis culture and carry out fermentation. After fermentation, sterilize, cool, centrifuge, and filter to obtain fermentation broth and fermentation residue. The inoculum size of Lactococcus lactis culture is 4% v / v, and the viable count is 3.5 × 10⁻⁶. 8 CFU / mL; fermentation temperature 30℃, oxygen supply 1%, time 36h; sterilization temperature 90℃, time 25min; S4. Add the fermentation residue obtained in step S3 to 7 times its mass of deionized water for ultrasonic extraction, centrifuge, and collect the supernatant; wherein, the ultrasonic extraction temperature is 35℃, the power is 200W, and the time is 30min. S5. Mix the fermentation broth obtained in step S3 and the supernatant collected in step S4, and freeze-dry to obtain the jasmine flower extract.

[0025] Comparative Example 9 The only difference between Comparative Example 9 and Example 1 is that the jasmine flower extract does not undergo lactococcal fermentation during the fermentation process. The specific preparation steps are as follows: S1. Mix jasmine flower powder with 12 times its mass of deionized water, sterilize, and cool to obtain the first fermentation substrate; wherein, the preparation method of the jasmine flower powder includes the following steps: washing jasmine flowers, first performing infrared drying, and then freeze-drying; wherein, the parameters for infrared drying are: vacuum degree of -0.08MPa, irradiation distance of 120mm, power density of 1.5W / g, infrared wavelength of 4μm, temperature of 40℃, and time of 50min; the sterilization temperature is 118℃ and the time is 20min; S2. Inoculate the first fermentation substrate with *Gastrodia elata* bacterial solution for fermentation. After fermentation, sterilize and cool to obtain the second fermentation substrate. The inoculum size of the *Gastrodia elata* bacterial solution is 5% v / v, and the viable count of the *Gastrodia elata* bacterial solution is 3 × 10⁻⁶. 8 The fermentation temperature was 26℃, the oxygen supply was 18%, and the time was 36h; the sterilization temperature was 90℃ and the time was 30min. S3. Inoculate the second fermentation substrate with Mycosaccharomyces cerevisiae culture and carry out fermentation. After fermentation, sterilize, cool, centrifuge, and filter to obtain fermentation broth and fermentation residue. The inoculum size of the Mycosaccharomyces cerevisiae culture is 4% v / v, and the viable cell count is 3.5 × 10⁻⁶. 8 CFU / mL; fermentation temperature 30℃, oxygen supply 1%, time 36h; sterilization temperature 90℃, time 25min; S4. Add the fermentation residue obtained in step S3 to 7 times its mass of deionized water for ultrasonic extraction, centrifuge, and collect the supernatant; wherein, the ultrasonic extraction temperature is 35℃, the power is 200W, and the time is 30min. S5. Mix the fermentation broth obtained in step S3 and the supernatant collected in step S4, and freeze-dry to obtain the jasmine flower extract.

[0026] Test Example 1: Inhibition Test of Each Composition on Tyrosinase Test method: Sample solutions: The compositions of Examples 1-5 and Comparative Examples 1-9 were dissolved in deionized water to prepare sample solutions with a concentration of 30 μg / mL.

[0027] Prepare a 0.5 mg / mL L-tyrosine solution and a 100 μg / mL tyrosinase solution using phosphate buffer (pH 6.8) as the solvent. Mix the tyrosine solution, tyrosinase solution, and sample solution thoroughly. Set up a blank control group. Incubate at 37°C for 30 min, and measure the absorbance at 475 nm. Calculate the inhibition rate of tyrosinase activity by the sample using the following formula: ; Sample group: 100 μL tyrosine solution + 50 μL sample solution + 50 μL tyrosinase solution; Sample blank group: 100 μL tyrosine solution + 50 μL sample solution + 50 μL buffer solution; Control group: 100 μL tyrosine solution + 50 μL tyrosinase + 50 μL buffer solution; Control group: 100 μL tyrosine solution + 100 μL buffer solution; Each group was measured an average of 3 times, and the average value was taken to calculate the tyrosinase inhibition rate. The results are shown in Table 2.

[0028] Test Example 2: Hyaluronidase Activity Inhibition Test of Each Composition Hyaluronidase is a hydrolytic enzyme that degrades hyaluronic acid. It is a major component of the extracellular matrix in connective tissue cells and is associated with most IgE-mediated type I and T-cell-mediated type IV hypersensitivity reactions. Therefore, in vitro hyaluronidase inhibition assays can, to some extent, reflect the skin-soothing, anti-allergic, and anti-inflammatory effects of corresponding products. A higher hyaluronidase inhibition rate indicates a better soothing effect of the composition.

[0029] The compositions of Examples 1-5 and Comparative Examples 1-9 were dissolved in deionized water to obtain sample solutions with a mass concentration of 6 mg / mL. In a 96-well plate, the sample solution was mixed with hyaluronidase solution (5 U / mL) by shaking and incubated at 37°C for 10 min. Then, sodium hyaluronate solution (0.03% w / v) was added and incubated at 37°C for 45 min. Subsequently, BSA solution (0.1% w / v) was added and incubated for another 10 min. OD was then measured. 600nm The value was used to determine the inhibitory effect of the test sample on hyaluronidase.

[0030] The formula for calculating the hyaluronidase activity inhibition rate is: Wherein, A is the OD600nm value of the control solution (using acetate buffer solution instead of the sample solution); B is the OD600nm value of the control blank solution (using acetate buffer solution instead of the sample solution and enzyme solution); C is the OD600nm value of the test sample solution; and D is the OD600nm value of the sample blank solution (using acetate buffer solution instead of the enzyme solution). See Table 2 for specific data.

[0031] Table 2. Tyrosinase inhibition rate and hyaluronidase inhibition rate of each composition Example 1 66.3 61.8 Example 2 64.2 60.3 Example 3 65.6 62.1 Example 4 60.8 57.5 Example 5 65.3 59.2 Comparative Example 1 44.8 39.6 Comparative Example 2 47.2 42.4 Comparative Example 3 41.7 39.1 Comparative Example 4 58.0 51.7 Comparative Example 5 53.2 47.4 Comparative Example 6 45.9 40.6 Comparative Example 7 50.4 45.2 Comparative Example 8 54.2 48.4 Comparative Example 9 51.6 46.0 As can be seen from the data of Examples 1-5 in Table 2, when the mass ratio of Tian Shan snow lotus cell culture, hydrolyzed silk protein and jasmine flower extract in the composition is in the range of (1.5-2):(0.5-1):(3-5), the whitening and soothing effects of the composition are at a better level.

[0032] Based on the data from Example 1 and Comparative Examples 1-3 in Table 2, it can be seen that the composition of Example 1, which uses a combination of three components—snow lotus cell culture, hydrolyzed silk protein, and jasmine flower extract—has higher whitening and soothing properties than Comparative Examples 1-3. This may be because the combination of snow lotus cell culture, hydrolyzed silk protein, and jasmine flower extract can synergistically enhance the whitening and soothing effects of the composition.

[0033] Based on the data from Example 1 and Comparative Examples 4-5 in Table 2, it can be seen that when the mass ratio of Tianshan snow lotus cell culture, hydrolyzed silk protein and jasmine flower extract is not within the range of (1-3):(0.3-1.5):(2-8), the whitening and soothing effects of the composition will be significantly reduced.

[0034] Combining the data from Example 1 and Comparative Examples 6-9 in Table 2, it can be seen that the whitening and soothing effects of the compositions in Comparative Examples 6-9 are significantly lower than those in Example 1. This may be because: in Comparative Example 6, the jasmine flower extract was not subjected to microbial fermentation, but only ultrasonic extraction was used, resulting in low degree of damage to the jasmine cell wall and insufficient release of whitening components such as polyphenols and flavonoids, as well as soothing components such as polysaccharides and terpenes. Therefore, the inhibition rates of tyrosinase and hyaluronidase were both at a low level. In Comparative Example 7, the strong aerobic fermentation step of *Gastrodia elata* was omitted, resulting in a significant reduction in the production of whitening active substances such as gluconic acid and gluconic acid in the system, and a significant decrease in the whitening effect. At the same time, insufficient acidification in the early stage also affected the metabolism of subsequent strains, and the soothing effect was reduced accordingly. In Comparative Example 8, the fermentation step of *Myriophyllum sp.* was omitted, resulting in insufficient degradation of the jasmine cell wall, insufficient release of active ingredients, and a reduction in the production of soothing and repairing substances such as extracellular polysaccharides. The whitening and soothing effects were weaker than those in Example 1. Comparative Example 9 omitted the Lactococcus lactis fermentation step, resulting in insufficient production of lactic acid and small molecule anti-inflammatory peptides in the system, significantly reducing the soothing effect. At the same time, the synergistic effect of the bacterial community was weakened, which also affected the full release of whitening active substances.

[0035] In summary, this invention, through a ternary compound system with a specific ratio combined with a gradient dissolved oxygen two-stage microbial fermentation process, can significantly improve the tyrosinase inhibitory activity and hyaluronidase inhibitory activity of the composition, achieving synergistic enhancement of whitening and brightening as well as soothing and repairing. The effect is significantly better than solutions with single extraction, single strain fermentation, missing components, or unbalanced ratios.

[0036] Application Example 1-5 and Comparative Application Example 1-9 The present invention provides an application example and a comparative application example of a serum with whitening and soothing effects, wherein the serum cream is composed of the following components by weight percentage: The composition comprises 1% whitening and soothing composition, 5% skin conditioning agent, 0.5% thickener, and 0.1 wt% preservative, with the balance being deionized water. The skin conditioning agent is a 1:1 mass ratio of glycerin and panthenol, the thickener is xanthan gum, and the preservative is phenazine. The whitening and soothing compositions used in Application Examples 1-5 are the whitening and soothing compositions prepared in Examples 1-5, respectively. The whitening and soothing compositions used in Comparative Application Examples 1-9 are the whitening and soothing compositions prepared in Comparative Examples 1-9, respectively. A Comparative Application Example 10 (blank application example) is also provided, the only difference between Comparative Application Example 10 and Application Example 1 being the absence of the whitening and soothing composition.

[0037] The preparation method of the above-mentioned whitening and soothing serum includes the following steps: A1: Mix 1 / 2 deionized water with the skin conditioning agent and the composition evenly to obtain mixture A; A2: Heat 1 / 3 of the deionized water to 60°C, then add the thickener and stir until well mixed to obtain mixture B; A3: Mix mixture A, mixture B cooled to 30°C, the remaining deionized water, and the preservative evenly to obtain each group of essences.

[0038] Test Example 3: Safety Tests of Various Serums Using the 2015 "Cosmetic Safety Technical Specifications" as a reference standard, the irritation of the serums corresponding to Case 1-5 and Comparative Application Case 1-10 was evaluated. The test method was a skin patch test, and 30 people aged 16-65 years were randomly distributed to participate in the test.

[0039] Test substance: the serums used in Examples 1-5 and Comparative Examples 1-10.

[0040] Test Method: An equal number of test areas as the amount of test substance were marked on the subject's back. The test substance was placed in a patch applicator (0.020-0.025g). The patch applicator containing the test substance was then covered with non-irritating adhesive tape on the subject's back. One test substance was assigned to each test area. The patch was gently pressed onto the skin surface with the palm of the hand to ensure even adhesion. The application was left for 24 hours. The patch applicator was removed 30 minutes later, and the skin reaction was observed after the pressure marks disappeared. If the result was negative, observations were repeated at 24 and 48 hours after the patch test. Evaluation was performed according to the evaluation criteria in Table 3.

[0041] Table 3 Evaluation criteria for skin patch test 0 negative reaction 1 Suspicious reaction, only slight erythema 2 Weakly positive reaction, erythema, infiltration, edema, and possibly papules. 3 Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area. 4 Extremely strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, reaction extending beyond the test area. Test results: Except for one Grade 1 reaction in control application example 8, all other groups showed negative skin reactions. These test results indicate that the serum provided by this invention is gentle and non-irritating to the skin and safe to use.

[0042] Test Example 4: Human Efficacy Test of Various Serums Subjects aged 40-55 with dull facial skin were selected and randomly divided into groups of 5. Each group used the serums from Application Examples 1-5 and Control Application Examples 1-10 for 4 weeks. The whitening and soothing effects were objectively evaluated using a testing device.

[0043] Researchers used a skin red-melanin analyzer to determine the melanin content of the cheeks; a skin color difference test probe to measure the skin whiteness (brightness) L of the cheeks; a skin red-melanin analyzer and test probe (DermaLab® Combo, Cortex, Denmark) to measure the skin melanin content MI of the cheeks; and a skin moisture loss analyzer to measure the transepidermal water loss (TEWL) value of the skin surface. During the test, the probe pressure and measurement time were fixed, and the same site was measured three times, and the average value was taken.

[0044] Among them: L value represents L* white balance, the larger the value, the more the color is white; the higher the MI measurement value, the higher the melanin content in the skin; the higher the TEWL value, the more transepidermal water loss the skin has. The higher the improvement rate of skin whiteness (L) and skin melanin (MI), the better the whitening effect of the serum. The higher the improvement rate of TEWL value, the better the soothing effect of the serum. The specific calculation formula is as follows: Skin whiteness L improvement rate = (L value) T0 -L value T28 ) / L value T0 ×100%; Skin melanin MI improvement rate = (MI) T0 -MI T28 ) / MI T0 ×100%; TEWL value improvement rate = (TEWL value) T0 -TEWL value T28 ) / TEWL value T0 ×100%; see Table 4 for specific data.

[0045] Table 4. Human efficacy tests of each group of serums Application Example 1 15.8 21.4 25.8 Application Example 2 14.0 18.3 23.7 Application Example 3 15.2 19.7 26.4 Application Example 4 12.6 15.1 21.6 Application Example 5 13.9 17.3 22.4 Comparative Application Example 1 5.4 6.6 12.8 Comparative Application Example 2 5.7 7.1 13.6 Comparative Application Example 3 5.0 6.3 12.2 Comparative Application Example 4 9.1 11.5 18.4 Comparative Application Example 5 8.6 10.7 17.2 Comparative Application Example 6 5.9 6.9 13.5 Comparative Application Example 7 8.0 10.1 16.7 Comparative Application Example 8 8.7 11.8 17.9 Comparative Application Example 9 8.2 10.4 16.1 Comparative Application Example 10 0.7 0.5 7.2 As shown in Table 4, the trends of the skin whiteness (L) improvement rate and skin melanin (MI) improvement rate of each serum are roughly similar to the tyrosinase activity inhibition rate of each composition, while the trend of the TEWL value improvement rate of the serum is roughly similar to the hyaluronidase activity inhibition rate of each composition. This indicates that the whitening and soothing composition of the present invention, through the scientific compounding of Tian Shan snow lotus cell culture, hydrolyzed silk protein, and specific three-stage fermented jasmine flower extract within a preferred mass ratio range, achieves a synergistic effect of inhibiting melanin production through multiple signaling pathways and anti-inflammatory and soothing effects at multiple targets. Compared with compositions prepared with single components, in non-preferred ratios, or without a complete fermentation process, the composition of the present invention significantly improves the whitening and soothing effects, proving the rationality of its formulation and the advancement of its process.

[0046] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A whitening and soothing composition, characterized in that, The product comprises the following components in parts by weight: Tian Shan snow lotus cell culture, hydrolyzed silk protein, and jasmine flower extract; the mass ratio of Tian Shan snow lotus cell culture, hydrolyzed silk protein, and jasmine flower extract is (1.5-2):(0.5-1):(3-5). The preparation method of the jasmine flower extract includes the following steps: S1. Mix dried jasmine powder with 10-15 times its weight of deionized water, sterilize, and cool to obtain the first fermentation substrate; S2. Inoculate the first fermentation substrate with *Gastrodia elata* bacterial solution for fermentation. After fermentation, sterilize and cool to obtain the second fermentation substrate. The inoculum size of the *Gastrodia elata* bacterial solution is 4-7% v / v, and the viable count of the *Gastrodia elata* bacterial solution is 10-1. 7 -10 9 CFU / mL; S3. Inoculate the second fermentation substrate with Mycosaccharomyces cerevisiae and Lactococcus lactis cultures, and carry out fermentation. After fermentation, sterilize, cool, centrifuge, and filter to obtain fermentation broth and fermentation residue. The total inoculum amount of Mycosaccharomyces cerevisiae and Lactococcus lactis cultures is 3-5% v / v, and the total viable count is 10. 7 -10 9 CFU / mL, the live count ratio of Saccharomyces cerevisiae to Lactococcus lactis is 1:(0.5-1.5). S4. Add the fermentation residue obtained in step S3 to 6-10 times its weight of deionized water for ultrasonic extraction, centrifuge, and collect the supernatant. S5. Mix the fermentation broth obtained in step S3 and the supernatant collected in step S4, and freeze-dry to obtain the jasmine flower extract.

2. The whitening and soothing composition as described in claim 1, characterized in that, The method for preparing the jasmine flower powder includes the following steps: washing the jasmine flowers, first performing infrared drying, and then performing freeze drying; wherein, the parameters for infrared drying are: vacuum degree of (-0.06)-(-0.09) MPa, irradiation distance of 100-150 mm, power density of 1-2 W / g, infrared wavelength of 3-5 μm, temperature of 35-45℃, and time of 30-60 min.

3. The whitening and soothing composition as described in claim 1, characterized in that, In step S1 of preparing jasmine flower extract, the sterilization temperature is 115-121℃ and the time is 15-40 min.

4. The whitening and soothing composition as described in claim 1, characterized in that, In step S2 of the preparation of jasmine flower extract, the fermentation temperature is 25-27℃, the oxygen supply is 15-20%, and the time is 24-72h; the sterilization temperature is 80-105℃, and the time is 20-40min.

5. The whitening and soothing composition as described in claim 1, characterized in that, In step S3 of the preparation of jasmine flower extract, the fermentation temperature is 26-32℃, the oxygen supply is 0.1-5%, and the time is 24-48h; the sterilization temperature is 80-105℃, and the time is 20-40min.

6. The whitening and soothing composition as described in claim 1, characterized in that, In step S4 of the preparation of jasmine flower extract, the ultrasonic extraction temperature is 30-40℃, the power is 100-300W, and the time is 20-40min.

7. The use of the whitening and soothing composition according to any one of claims 1-6 in the preparation of cosmetics.

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