Method for treating aquaculture wastewater with antibiotics by microalgae and bacteria in cooperation

By using a microalgae-bacteria symbiotic granular sludge system with Chlorella as the core, and by utilizing pre-screened tetracycline-degrading bacteria and light-dark alternation technology, the problems of uncontrollable antibiotic-degrading bacteria and carbon source dependence in existing technologies have been solved, achieving simultaneous and efficient removal of tetracycline, ammonia nitrogen and total phosphorus from aquaculture wastewater and biomass resource utilization.

CN122276992APending Publication Date: 2026-06-26HUANGSHAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUANGSHAN UNIV
Filing Date
2026-05-08
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

In existing microalgae-bacterial granular sludge systems, the function of antibiotic-degrading bacteria is uncontrollable when treating aquaculture wastewater, the carbon source depends on external addition, and the three treatment functions lack spatial coupling optimization, making it impossible to achieve simultaneous and efficient removal of tetracycline, ammonia nitrogen, and total phosphorus.

Method used

Using Chlorella microalgae as the core of the granular structure and pre-screened tetracycline-degrading dominant bacterial groups as directional functional components, algae-bacteria symbiotic granular sludge is formed through a self-assembly process driven by alternating light and dark and sedimentation selection pressure. Oxygen is produced by the photosynthesis of microalgae to achieve the simultaneous removal of tetracycline, ammonia nitrogen and total phosphorus. A closed-loop cycle of O2/CO2/DOC is formed through algae-bacteria symbiosis, avoiding the need for external carbon sources.

Benefits of technology

Without the addition of an external carbon source, a tetracycline removal rate of 92%, ammonia nitrogen removal rate of 97%, and total phosphorus removal rate of 88% were achieved. The effluent quality met the discharge standards for aquaculture tailwater, and the algae and bacteria biomass could be used as feed protein raw materials, achieving the dual benefits of wastewater treatment and biomass resource utilization.

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Abstract

This invention discloses a method for the synergistic treatment of antibiotics in aquaculture wastewater by microalgae and bacteria, belonging to the field of biological treatment technology for aquaculture wastewater. Microalgae of the genus *Chlorella* and a dominant tetracycline-degrading bacterial community obtained through tetracycline concentration gradient acclimation and screening from activated sludge in aquaculture farms are co-inoculated in a sequencing batch reactor (SBR) at an algae-bacterial cell ratio of 3-8:1. Through alternating light and dark operation and selective pressure with gradually shortened settling time, the algae and bacteria self-assemble to form algae-bacterial symbiotic granular sludge. Under conditions without external carbon source, the simultaneous and efficient removal of tetracycline antibiotics, ammonia nitrogen, and total phosphorus from aquaculture wastewater is achieved through a closed-loop O2 / CO2 / DOC cycle of algae and bacteria within the granules. Under optimal conditions, the tetracycline removal rate reaches 92%, the ammonia nitrogen removal rate reaches 97%, and the total phosphorus removal rate reaches 88%. The harvested biomass has a protein content of not less than 44%, which can be used as feed protein raw material, achieving the dual benefits of wastewater treatment and resource recovery.
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