An organic fertilizer composition based on a high-yield urease inhibitor

Abstract

This invention provides an organic fertilizer composition based on a high-yield urease inhibitor, belonging to the field of organic fertilizer technology. The organic fertilizer includes soybean meal, rice bran, straw, compound microbial inoculant, livestock and poultry manure, cassava residue, furfural residue, peanut shells, wood ash, edible mushroom residue, humic acid, peat powder, chitosan, diatomaceous earth, urease inhibitor, compound enzyme preparation, and compound amino acids. This invention uses soybean meal, rice bran, and straw as the main components. First, the mixture is pretreated with a compound enzyme preparation, then inoculated with compound amino acids and microbial inoculant for the first stage of fermentation. After the first stage of fermentation, the mixture is mixed with the prescribed amounts of livestock and poultry manure, cassava residue, humic acid, etc., for the second stage of fermentation. After the two stages of fermentation and maturation, the mixture is blended with minerals such as diatomaceous earth and peat powder, and the prescribed amount of urease inhibitor is added to obtain the finished product. Thanks to the formulation of this invention and the synergistic effect with the urease inhibitor, the fertilizer efficiency is significantly improved, resulting in a more prominent growth-promoting effect on crops.

Description

Technical Field

[0001] This invention relates to the field of organic fertilizer technology, specifically to an organic fertilizer composition based on a high-yield urease inhibitor. Background Technology

[0002] Organic fertilizer refers to fertilizer made primarily from animal excrement or by-products rich in organic matter, which are fermented and decomposed. Organic fertilizer is characterized by its ability to improve soil quality, enhance soil fertility, increase soil nutrient activity, purify the soil environment, and ensure high-quality, high-yield, and high-efficiency vegetable production. It is an irreplaceable fertilizer for greenhouse vegetable cultivation. The raw materials for organic fertilizer production are diverse, mainly including agricultural waste (such as straw, soybean meal, cottonseed meal, and rapeseed cake), livestock and poultry manure (such as chicken manure, cattle, sheep, horse manure, and rabbit manure), industrial waste (such as distiller's grains, vinegar residue, cassava residue, sugar residue, furfural residue, and sawdust), domestic waste (such as kitchen waste and vegetable market leftovers), urban sludge (such as river silt and sewer silt), and plant products (such as bamboo powder, bamboo charcoal, bamboo ash, straw charcoal, and wood ash).

[0003] The typical production process of organic fertilizer includes raw material pretreatment, fermentation, turning, composting, and screening. For raw materials such as livestock and poultry manure, crop straw, and kitchen waste, preliminary crushing and mixing are required to ensure uniform fermentation. For raw materials with high moisture content (such as fresh manure), auxiliary materials (such as straw and sawdust) need to be added to adjust the moisture content to 50%-60%. Based on this, the pretreated raw materials are piled into windrows or placed in fermentation tanks, and fermentation agents (such as EM bacteria and Bacillus subtilis) are added to decompose organic matter through microbial action. During fermentation, the temperature needs to be controlled at 55-70℃ for 7-15 days to kill pathogens and weed seeds. The pile needs to be turned regularly during fermentation (usually every 2-3 days) to increase oxygen supply and promote microbial activity. The frequency and number of turnings depend on temperature changes and the characteristics of the raw materials. After fermentation, the material needs to be left to compost for 20-30 days to further stabilize the organic matter. The composted material should be odorless, dark brown, and loose in texture. After composting, the material is screened to remove impurities (such as stones, plastics, etc.) and the particle size (powder or granules) is adjusted as needed.

[0004] The selection of raw materials and production processes have a crucial impact on the performance and efficacy of organic fertilizers. Currently, the efficacy of conventional organic fertilizers still needs improvement, and while they have some growth-promoting effect on crops, the effect is relatively limited. Urease inhibitors are chemical agents that can inhibit urease activity in the soil and delay urea hydrolysis. Soil urease is a specific hydrolytic enzyme in the soil that catalyzes the hydrolysis of urea. The mechanism by which urease inhibitors control urea hydrolysis mainly involves two aspects: first, their oxidation reduces urease activity; second, they compete for ligands to reduce urease activity. Several specialized fertilizers containing slow-release nitrogen fertilizers such as hydroquinone and dicyandiamide have been widely applied. Urease inhibitors can also be used as feed additives for ruminants, effectively reducing ammonia levels in poultry houses, improving the environment, and increasing the efficiency of nitrogen utilization by animals. Currently, some researchers have applied urease inhibitors to fertilizer products to improve fertilizer performance; however, conventional addition methods have failed to maximize the effect of urease inhibitors, and the overall performance of the resulting fertilizer products is still not ideal. Summary of the Invention

[0005] The present invention aims to address the technical deficiencies of the prior art by providing an organic fertilizer composition based on a high-yield urease inhibitor, thereby solving the technical problem that the performance of conventional organic fertilizer products needs to be improved.

[0006] To achieve the above technical objectives, the present invention adopts the following technical solution: An organic fertilizer composition based on a high-yield urease inhibitor comprises the following ingredients: soybean meal, rice bran, straw, compound microbial agent, livestock and poultry manure, cassava residue, furfural residue, peanut shells, wood ash, edible fungus residue, humic acid, peat powder, chitosan, diatomaceous earth, urease inhibitor, compound enzyme preparation, and compound amino acids.

[0007] Preferably, each gram of the compound microbial agent contains the following live bacteria: 0.5~1×10⁻⁶ Lactobacillus plantarum. 8 CFU, Bacillus belye 0.2~1×10 7 CFU, Bacillus licheniformis 0.2~1×10 7 CFU, brewer's yeast 0.5~1×10 8 CFU, Alcaligenes mica 0.2~0.5×10 8 CFU, Aspergillus niger 0.2~1×10 7 CFU.

[0008] Preferably, each g of the compound enzyme preparation contains the following enzymes: bromelain 210~250 U / g, papain 450~500 U / g, cyclodextrin glucoside transferase 400~450 U / g, hexose oxidase 100~150 U / g, xylanase 60~100 U / g, and pectin lyase 100~150 U / g.

[0009] Preferably, each g of the compound amino acid contains the following amino acids: glycine 0.1~0.2g, proline 0.1~0.2g, alanine 0.1~0.2g, tryptophan 0.1~0.2g, methionine 0.1~0.2g, and isoleucine 0.1~0.2g.

[0010] Preferably, the organic fertilizer composition comprises the following components in parts by weight: 30-40 parts soybean meal, 20-30 parts rice bran, 30-35 parts straw, 3-5 parts compound microbial inoculant, 10-15 parts livestock and poultry manure, 8-10 parts cassava residue, 8-10 parts furfural residue, 5-8 parts peanut shells, 5-8 parts wood ash, 8-10 parts edible fungus residue, 1-3 parts humic acid, 5-7 parts peat powder, 2-4 parts chitosan, 8-10 parts diatomaceous earth, 0.5-1 part urease inhibitor, 1-2 parts compound enzyme preparation, and 1-2 parts compound amino acids.

[0011] Preferably, the organic fertilizer composition comprises the following components in parts by weight: 35 parts soybean meal, 25 parts rice bran, 33 parts straw, 4 parts compound microbial inoculant, 12 parts livestock and poultry manure, 9 parts cassava residue, 9 parts furfural residue, 7 parts peanut shells, 6 parts wood ash, 9 parts edible fungus residue, 2 parts humic acid, 6 parts peat powder, 3 parts chitosan, 9 parts diatomaceous earth, 0.8 parts urease inhibitor, 1.5 parts compound enzyme preparation, and 1.5 parts compound amino acids.

[0012] Preferably, the organic fertilizer composition also includes 1 to 3 parts by weight of bamboo charcoal powder.

[0013] Preferably, the organic fertilizer composition also includes 2 to 5 parts by weight of zeolite powder.

[0014] Preferably, the organic fertilizer composition also includes 1 to 2 parts by weight of rice husks.

[0015] Preferably, the organic fertilizer composition comprises the following components in parts by weight: 35 parts soybean meal, 25 parts rice bran, 33 parts straw, 4 parts compound microbial inoculant, 12 parts livestock and poultry manure, 9 parts cassava residue, 9 parts furfural residue, 7 parts peanut shells, 6 parts wood ash, 9 parts edible fungus residue, 2 parts humic acid, 6 parts peat powder, 3 parts chitosan, 9 parts diatomaceous earth, 0.8 parts urease inhibitor, 1.5 parts compound enzyme preparation, 1.5 parts compound amino acids, 2 parts bamboo charcoal powder, 3 parts zeolite powder, and 1.5 parts rice husk.

[0016] This invention provides an organic fertilizer composition based on a high-yield urease inhibitor. The technical solution uses soybean meal, rice bran, and straw as the main components. First, the mixture is pretreated with a compound enzyme preparation, followed by the incorporation of compound amino acids and microbial agents for initial fermentation. After the initial fermentation, a formula-formulated amount of livestock and poultry manure, cassava residue, furfural residue, humic acid, and other components are mixed in for a second fermentation. After these two stages of fermentation and decomposition, the nutrients are more readily absorbed and utilized by crops. Based on this, the mixture is blended with minerals such as diatomaceous earth and peat powder, and a formula-formulated amount of urease inhibitor is added to obtain the finished product.

[0017] The urease inhibitor used in this invention can alter the redox conditions of the soil's microecological environment, reduce soil urea activity, decrease the water solubility of urea, slow down the hydrolysis rate of urea, and disrupt the metabolic pathways of microorganisms that produce urease, thus hindering the synthesis of urease and reducing the distribution density of urease in the soil, thereby slowing down the decomposition rate of urea. Thanks to the formulation and fermentation process of this invention, as well as the synergistic effect with the urease inhibitor, fertilizer efficiency is significantly improved, resulting in a more pronounced growth-promoting effect on crops. Attached Figure Description

[0018] Figure 1 This is a statistical result chart of the cultivation effects of various embodiments and comparative examples of the present invention. Detailed Implementation

[0019] The specific embodiments of the present invention will be described in detail below. To avoid excessive and unnecessary detail, well-known structures or functions will not be described in detail in the following embodiments. The approximate language used in the following embodiments is for quantitative purposes, indicating that a certain degree of variation in quantity is permissible without changing the basic function. Unless otherwise defined, the technical and scientific terms used in the following embodiments have the same meaning as commonly understood by those skilled in the art to which this invention pertains.

[0020] Example 1 An organic fertilizer composition based on a high-yield urease inhibitor comprises the following components in parts by weight: 35 parts soybean meal, 25 parts rice bran, 33 parts straw, 4 parts compound microbial inoculant, 12 parts livestock and poultry manure, 9 parts cassava residue, 9 parts furfural residue, 7 parts peanut shells, 6 parts wood ash, 9 parts edible fungus residue, 2 parts humic acid, 6 parts peat powder, 3 parts chitosan, 9 parts diatomaceous earth, 0.8 parts urease inhibitor, 1.5 parts compound enzyme preparation, and 1.5 parts compound amino acids.

[0021] Each gram of the compound microbial agent contains the following live bacteria: Lactobacillus plantarum 0.6 × 10⁻⁶. 8 CFU, Bacillus belye 0.7 × 10 7 CFU, Bacillus licheniformis 0.5 × 10⁻⁶ 7CFU, brewer's yeast 0.8×10 8 CFU, Alcaligenes mica 0.4 × 10⁻⁶ 8 CFU, Aspergillus niger 0.6×10 7 CFU. Each gram of the compound enzyme preparation contains the following enzymes: bromelain 220 U / g, papain 480 U / g, cyclodextrin glucoside transferase 420 U / g, hexose oxidase 130 U / g, xylanase 80 U / g, and pectin lyase 120 U / g. Each gram of the compound amino acid contains the following amino acids: glycine 0.15 g, proline 0.15 g, alanine 0.1 g, tryptophan 0.1 g, methionine 0.2 g, tryptophan 0.1 g, and isoleucine 0.2 g.

[0022] The preparation method is as follows: Crush and mix the soybean meal, rice bran, and straw according to the formula, add the compound enzyme preparation according to the formula, stir for 2-3 hours, then add the compound microbial agent and compound amino acids according to the formula, control the temperature at 28-32℃, and ferment for 2-3 days; mix in the livestock and poultry manure, cassava residue, furfural residue, peanut shells, wood ash, edible fungus residue, humic acid, and chitosan according to the formula, adjust the temperature to 26-28℃, and continue fermentation for 3-5 days; dry the fermentation product naturally to a moisture content of 15%, add the peat powder, diatomaceous earth, and urease inhibitor according to the formula, and mix well.

[0023] Cultivation experiments were conducted in greenhouses. Each embodiment or comparative example had three replicate areas, each with an area of ​​1m × 1m = 1m². 2 The same number and variety of cabbage seedlings are planted in each area.

[0024] Nutrient content: The content of nitrogen, phosphorus, potassium and other components in organic fertilizer was determined by chemical analysis methods.

[0025] Crop growth indicators: Plant height: Use a ruler to regularly measure the height of each cabbage plant from the root to the top, record the data at different growth stages, and finally calculate the average value.

[0026] Stem diameter: Measure the diameter of the base of the stem of each cabbage plant using vernier calipers, and take the average value after multiple measurements.

[0027] Leaf area: The maximum leaf area of ​​each cabbage plant was measured using a leaf area meter, and the average leaf area was calculated.

[0028] Biomass: After the cabbage has finished growing, the plants are removed from the soil, washed, blanched at 105℃ for 15 minutes, and then dried at 60℃ to constant weight. The dry matter mass is weighed and recorded, and the average value is calculated.

[0029] The organic fertilizer in this example was tested and found to have an organic matter content of 66.52%, a total nitrogen content of 421.3 g / kg, a available nitrogen content of 208.5 g / kg, a available phosphorus content of 86.2 g / kg, and a available potassium content of 101.4 g / kg. The cultivated Chinese cabbage plants reached a height of 55.7 cm, a stem diameter of 28.2 cm, and a leaf area of ​​59.3 cm². 2 The biomass is 1.81 kg / plant.

[0030] Example 2 An organic fertilizer composition based on a high-yield urease inhibitor comprises the following components in parts by weight: 30 parts soybean meal, 20 parts rice bran, 30 parts straw, 3 parts compound microbial inoculant, 10 parts livestock and poultry manure, 8 parts cassava residue, 8 parts furfural residue, 5 parts peanut shells, 5 parts wood ash, 8 parts edible fungus residue, 1 part humic acid, 5 parts peat powder, 2 parts chitosan, 8 parts diatomaceous earth, 0.5 parts urease inhibitor, 1 part compound enzyme preparation, and 1 part compound amino acids.

[0031] The components of the compound microbial agent, compound enzyme preparation, and compound amino acid are the same as in Example 1, and the preparation method is the same as in Example 1.

[0032] The organic fertilizer in this example was tested and found to have an organic matter content of 67.29%, a total nitrogen content of 431.1 g / kg, a available nitrogen content of 212.4 g / kg, a available phosphorus content of 90.3 g / kg, and a available potassium content of 103.2 g / kg. The cultivated Chinese cabbage plants reached a height of 57.1 cm, a stem diameter of 29.0 cm, and a leaf area of ​​61.3 cm². 2 The biomass is 1.94 kg / plant.

[0033] Example 3 An organic fertilizer composition based on a high-yield urease inhibitor comprises the following components in parts by weight: 40 parts soybean meal, 30 parts rice bran, 35 parts straw, 5 parts compound microbial inoculant, 15 parts livestock and poultry manure, 10 parts cassava residue, 10 parts furfural residue, 8 parts peanut shells, 8 parts wood ash, 10 parts edible fungus residue, 3 parts humic acid, 7 parts peat powder, 4 parts chitosan, 10 parts diatomaceous earth, 1 part urease inhibitor, 2 parts compound enzyme preparation, and 2 parts compound amino acids.

[0034] The components of the compound microbial agent, compound enzyme preparation, and compound amino acid are the same as in Example 1, and the preparation method is the same as in Example 1.

[0035] The organic fertilizer in this example was tested and found to have an organic matter content of 64.33%, a total nitrogen content of 445.1 g / kg, a available nitrogen content of 212.6 g / kg, a available phosphorus content of 91.5 g / kg, and a available potassium content of 100.1 g / kg. The cultivated Chinese cabbage plants reached a height of 58.1 cm, a stem diameter of 27.3 cm, and a leaf area of ​​55.2 cm².2 The biomass is 1.69 kg / plant.

[0036] Example 4 An organic fertilizer composition based on a high-yield urease inhibitor comprises the following components in parts by weight: 35 parts soybean meal, 25 parts rice bran, 33 parts straw, 4 parts compound microbial inoculant, 12 parts livestock and poultry manure, 9 parts cassava residue, 9 parts furfural residue, 7 parts peanut shells, 6 parts wood ash, 9 parts edible fungus residue, 2 parts humic acid, 6 parts peat powder, 3 parts chitosan, 9 parts diatomaceous earth, 0.8 parts urease inhibitor, 1.5 parts compound enzyme preparation, 1.5 parts compound amino acids, 2 parts bamboo charcoal powder, 3 parts zeolite powder, and 1.5 parts rice husk.

[0037] The components of the compound microbial agent, compound enzyme preparation, and compound amino acid are the same as in Example 1, and the preparation method is the same as in Example 1.

[0038] The organic fertilizer in this example was tested and found to have an organic matter content of 69.18%, a total nitrogen content of 408.5 g / kg, a available nitrogen content of 214.6 g / kg, a available phosphorus content of 91.2 g / kg, and a available potassium content of 95.7 g / kg. The cultivated Chinese cabbage plants reached a height of 60.7 cm, a stem diameter of 30.1 cm, and a leaf area of ​​58.4 cm². 2 The biomass is 1.90 kg / plant.

[0039] Comparative Example 1 Junbao Brothers brand bio-organic fertilizer products are produced by Chengdu Huisen Biotechnology Co., Ltd.

[0040] The organic matter content was measured to be 62.05%, total nitrogen content to be 376.2 g / kg, available nitrogen content to be 198.1 g / kg, available phosphorus content to be 79.5 g / kg, and available potassium content to be 83.4 g / kg. The cultivated cabbage plants reached a height of 48.6 cm, a stem diameter of 22.3 cm, and a leaf area of ​​50.2 cm². 2 The biomass is 1.43 kg / plant.

[0041] Comparative Example 2 Fuming brand bio-organic fertilizer products are produced by Shijiazhuang Fuming Bio-organic Fertilizer Co., Ltd.

[0042] The organic matter content was measured to be 58.05%, total nitrogen content to be 352.1 g / kg, available nitrogen content to be 182.6 g / kg, available phosphorus content to be 83.7 g / kg, and available potassium content to be 80.1 g / kg. The cultivated cabbage plants reached a height of 45.2 cm, a stem diameter of 23.1 cm, and a leaf area of ​​52.3 cm². 2 The biomass is 1.41 kg / plant.

[0043] Comparative Example 3 Based on Example 4, the urease inhibitor was removed.

[0044] This comparative example of organic fertilizer was tested and found to contain 68.85% organic matter, 409.2 g / kg total nitrogen, 216.7 g / kg available nitrogen, 90.6 g / kg available phosphorus, and 96.9 g / kg available potassium. The cultivated Chinese cabbage plants reached a height of 53.1 cm, a stem diameter of 24.2 cm, and a leaf area of ​​53.9 cm². 2 The biomass is 1.55 kg / plant.

[0045] The embodiments of the present invention have been described in detail above, but the content described is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the scope of the present invention should be included within the protection scope of the present invention.

Claims

1. An organic fertilizer composition based on a high-yield urease inhibitor, characterized in that... It includes the following ingredients: soybean meal, rice bran, straw, compound microbial inoculant, livestock and poultry manure, cassava residue, furfural residue, peanut shells, wood ash, edible fungus residue, humic acid, peat powder, chitosan, diatomaceous earth, urease inhibitor, compound enzyme preparation, and compound amino acids.

2. The organic fertilizer composition based on a high-yield urease inhibitor according to claim 1, characterized in that, Lactobacillus plantarum 0.5~1 x 10 8 cfu, Bacillus velezensis 0.2~1 x 10 7 cfu, Bacillus licheniformis 0.2~1 x 10 7 cfu, Saccharomyces cerevisiae 0.5~1 x 10 8 cfu, Alcaligenes faecalis 0.2~0.5 x 10 8 cfu, Aspergillus niger 0.2~1 x 10 7 cfu.

3. The organic fertilizer composition based on a high-yield urease inhibitor according to claim 2, characterized in that, Each gram of the compound enzyme preparation contains the following enzymes: bromelain 210~250 U / g, papain 450~500 U / g, cyclodextrin glucoside transferase 400~450 U / g, hexose oxidase 100~150 U / g, xylanase 60~100 U / g, and pectin lyase 100~150 U / g.

4. The organic fertilizer composition based on a high-yield urease inhibitor according to claim 3, characterized in that, Each gram of the compound amino acid contains the following amino acids: glycine 0.1~0.2g, proline 0.1~0.2g, alanine 0.1~0.2g, tryptophan 0.1~0.2g, methionine 0.1~0.2g, and isoleucine 0.1~0.2g.

5. The organic fertilizer composition based on a high-yield urease inhibitor according to claim 4, characterized in that, The organic fertilizer composition comprises the following components in parts by weight: 30-40 parts soybean meal, 20-30 parts rice bran, 30-35 parts straw, 3-5 parts compound microbial inoculant, 10-15 parts livestock and poultry manure, 8-10 parts cassava residue, 8-10 parts furfural residue, 5-8 parts peanut shells, 5-8 parts wood ash, 8-10 parts edible fungus residue, 1-3 parts humic acid, 5-7 parts peat powder, 2-4 parts chitosan, 8-10 parts diatomaceous earth, 0.5-1 part urease inhibitor, 1-2 parts compound enzyme preparation, and 1-2 parts compound amino acids.

6. The organic fertilizer composition based on a high-yield urease inhibitor according to claim 5, characterized in that, The organic fertilizer composition comprises the following components in parts by weight: 35 parts soybean meal, 25 parts rice bran, 33 parts straw, 4 parts compound microbial inoculant, 12 parts livestock and poultry manure, 9 parts cassava residue, 9 parts furfural residue, 7 parts peanut shells, 6 parts wood ash, 9 parts edible fungus residue, 2 parts humic acid, 6 parts peat powder, 3 parts chitosan, 9 parts diatomaceous earth, 0.8 parts urease inhibitor, 1.5 parts compound enzyme preparation, and 1.5 parts compound amino acids.

7. The organic fertilizer composition based on a high-yield urease inhibitor according to claim 5, characterized in that, The organic fertilizer composition also includes 1 to 3 parts by weight of bamboo charcoal powder.

8. The organic fertilizer composition based on a high-yield urease inhibitor according to claim 5, characterized in that, The organic fertilizer composition also includes 2 to 5 parts by weight of zeolite powder.

9. The organic fertilizer composition based on a high-yield urease inhibitor according to claim 5, characterized in that, The organic fertilizer composition also includes 1 to 2 parts by weight of rice husks.

10. An organic fertilizer composition based on a high-yield urease inhibitor according to claim 5, characterized in that, This organic fertilizer composition consists of the following components in parts by weight: 35 parts soybean meal, 25 parts rice bran, 33 parts straw, 4 parts compound microbial inoculant, 12 parts livestock and poultry manure, 9 parts cassava residue, 9 parts furfural residue, 7 parts peanut shells, 6 parts wood ash, 9 parts edible fungus residue, 2 parts humic acid, 6 parts peat powder, 3 parts chitosan, 9 parts diatomaceous earth, 0.8 parts urease inhibitor, 1.5 parts compound enzyme preparation, 1.5 parts compound amino acids, 2 parts bamboo charcoal powder, 3 parts zeolite powder, and 1.5 parts rice husk.

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