Sampling mechanism for bioreactor vessels

Abstract

This invention discloses a sampling mechanism for a bioreactor, comprising: a material chamber formed within the reactor body; a valve body connector and a sampling pipe fixedly installed at the top of the reactor body; the valve body connector is further equipped with a steam sterilization mechanism for steam sterilization and a partition sampling mechanism for sampling; the partition sampling mechanism includes a sterilization sleeve with a sterilization chamber within it; a diversion pipe fixedly installed at the bottom of the sterilization sleeve; a partition chamber fixedly installed within the sterilization chamber; a partition sphere slidably connected within the partition chamber; and a sampling connector pipe fixedly installed at one end of the sterilization sleeve. In use, the sampling mechanism of this invention uses the sampling connector pipe in conjunction with a gas-gathering hopper to converge gas and push the partition sphere to slide, thus opening the sampling channel. After sampling, the partition sphere falls back under its own weight, blocking the diversion pipe, achieving both closed-loop aseptic sampling and effectively preventing backflow of external impurities into the reactor body.

Description

Technical Field

[0001] This invention relates to the field of bioreactor sampling technology, and more specifically to a sampling mechanism for bioreactors. Background Technology

[0002] Bioreactors are core equipment for cell / microbial culture in the fields of pharmaceuticals, bioengineering, and food fermentation.

[0003] Bioreactors, as core equipment in bioengineering fields such as biopharmaceuticals, fermentation engineering, and microbial culture, are mainly used to provide suitable reaction environments such as temperature, pressure, and pH for microorganisms, cells, or biological enzymes to achieve the transformation of biological substrates, product synthesis, or cultivation of bioactive substances. Currently, gas sampling in bioreactors is usually achieved through sampling valves installed on the side wall or top of the reactor body. Most existing sampling valves adopt a single gas path structure, that is, the sampling channel is directly connected to the inside of the reactor body. When sampling, the valve is opened to allow the gas inside the tank to flow out along a single channel to the sampling container. After sampling, the valve is closed to complete the operation.

[0004] Since the gas sampling process in the bioreactor is achieved by the pressure difference between the inside of the tank and the outside, as the gas sample is continuously released, the gas pressure inside the tank will gradually decrease. When the pressure inside the tank is lower than the outside atmospheric pressure, if the sampling valve is not closed in time, microorganisms, dust and other impurities in the outside air will be drawn back into the tank under the action of the pressure difference. In order to ensure the sterility of the sampling process, the sampling pipeline and sampling valve need to be sterilized by high temperature steam in a steam sterilization chamber regularly. Since the existing sampling valve adopts a single gas path design, during the sterilization process, the high temperature steam cannot form an effective barrier after flowing through the sampling valve, and some steam will flow back into the tank through the single gas path. Summary of the Invention

[0005] The main objective of this invention is to provide a sampling mechanism for bioreactors to solve the problems in the prior art, such as the pressure inside the bioreactor decreasing as the gas is released, which can easily lead to backflow of external impurities and contamination if the valve is not closed in time when gas sampling is performed, and the fact that the existing sampling valve is a single gas path and the sterilization steam cannot be effectively blocked, which can easily flow back into the reactor body and cause contamination.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a sampling mechanism for a bioreactor, comprising: The material chamber, used for placing materials, is located inside the reaction vessel body; The valve body connector and the sampling tube are fixedly installed at the top of the reaction vessel. The end of the sampling tube away from the valve body connector extends into the material chamber. The valve body connector is also provided with a steam sterilization mechanism for steam sterilization and a partition sampling mechanism for sampling. The isolation sampling mechanism includes a sterilization sleeve fixedly installed on the valve body connector and located outside the reaction vessel. The sterilization sleeve has a sterilization chamber. A diversion pipe is fixedly installed at the bottom end of the sterilization sleeve, and the sterilization chamber is connected to a sampling pipe through the diversion pipe. An isolation chamber distributed along the axial direction of the sterilization sleeve is also fixedly installed in the sterilization chamber. An isolation cavity connected to the diversion pipe is opened in the isolation chamber. An isolation ball for sealing the diversion pipe is also slidably connected in the isolation chamber. A sampling connector pipe connected to the sterilization chamber is also fixedly installed at one end of the sterilization sleeve that protrudes from the reaction vessel body.

[0007] In one embodiment, the partition chamber is provided with an integrally formed air-gathering hopper at one end near the diversion pipe. The air-gathering hopper is funnel-shaped and connected to the partition sphere with an interference fit. A ball cap is fixedly installed on the inner wall of the sterilization sleeve at the end of the partition chamber away from the air-gathering hopper. The ball cap has an arc-shaped surface that matches the partition sphere.

[0008] In one embodiment, the partition chamber is further provided with a sampling hole, which is arranged to avoid each other with the ball cap. The partition cavity is connected to the sterilization cavity through the sampling hole, and a steam channel connected to the partition cavity is fixedly installed on the sterilization sleeve.

[0009] In one embodiment, the valve body connector has a first air chamber connected to the sampling tube, and a steam connector pipe for connecting to a steam sterilization device is also fixedly installed on the valve body connector, with one end of the steam connector pipe passing through the valve body connector located in the first air chamber.

[0010] In one embodiment, the first air chamber is connected to the partition chamber via a steam channel, which extends through one end of the sterilization chamber into the partition chamber.

[0011] In one embodiment, the steam sterilization mechanism includes a vent valve body fixedly installed at the top of the valve body connector. The vent valve body has a second air chamber that is concentrically distributed with the first air chamber, and the second air chamber is connected to the first air chamber through a venting channel.

[0012] In one embodiment, a pressure relief safety chamber is also fixedly installed outside the vent valve. The pressure relief safety chamber has vent holes arranged in a ring array that communicate with the second air chamber, and the diameter of the second air chamber gradually increases from the outside to the inside.

[0013] In one embodiment, the vent valve body is further slidably connected to an airtight piston for sealing the sampling tube and the first air chamber, and an airtight spring for resetting the airtight piston is provided in the vent valve body and outside the airtight piston.

[0014] In one embodiment, the airtight piston has vertically distributed connecting rod ends along its axial direction. The top end of the connecting rod ends extends to the body of the vent valve body. The two ends of the connecting rod ends are respectively fixedly installed with a first plug for sealing the first air chamber and a second plug for sealing the second air chamber. The end of the first plug extending into the sampling tube is tapered, and the second plug is interference-fitted with the depressurization safety chamber.

[0015] In one embodiment, the two ends of the airtight spring are respectively connected to the inner wall of the vent valve body and the second plug.

[0016] Compared with the prior art, the present invention has at least the following beneficial effects: (1) The present invention achieves the connection of the sampling channel by using the sampling connector tube in conjunction with the gas gathering bucket to gather gas and push the partition ball to slide. After sampling, the partition ball falls back under gravity to block the diversion tube, which not only achieves closed sterile sampling, but also effectively prevents external impurities from being drawn back and contaminating the reaction tank.

[0017] (2) The present invention forms a first seal by sealing the sampling tube with the first plug of the airtight piston and sealing the second air chamber with the second plug. The second seal is formed by the interference fit between the partition ball and the gas gathering bucket. At the same time, the steam is divided into two paths through the steam connector pipe to sterilize the core components such as the partition chamber and sterilization chamber and the pipeline, so that the sterilization effect is more thorough.

[0018] (3) The present invention uses the coordinated design of airtight piston, airtight spring and pressure relief safety chamber. When the pressure inside the reaction tank is higher than the rated pre-tightening force of the airtight spring, the airtight piston is pushed upward to unlock the airflow channel. The excess pressure is evenly discharged through the second air chamber with varying aperture and the annular vent hole. This helps the reaction tank to have its own safety pressure relief structure to avoid damage to the equipment due to excessive air pressure. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0020] Figure 1 This is a three-dimensional structural schematic diagram of a sampling mechanism for a bioreactor according to an embodiment of the present invention; Figure 2 This is a cross-sectional perspective view of a sampling mechanism for a bioreactor according to an embodiment of the present invention; Figure 3 This is a structural diagram of the installation of a steam sterilization mechanism and a partition sampling mechanism in one embodiment of the present invention; Figure 4This is a cross-sectional view of the installation of a steam sterilization mechanism and a partition sampling mechanism in one embodiment of the present invention. Figure 5 This is a cross-sectional view of a valve body connector and a vent valve body being installed together in one embodiment of the present invention; Figure 6 This is a cross-sectional view of a partition sampling mechanism according to an embodiment of the present invention; Figure 7 for Figure 2 A magnified structural diagram of part A in the middle.

[0021] Explanation of reference numerals in the attached figures: 1. Reaction vessel body; 2. Material chamber; 3. Valve body connector; 31. First gas chamber; 311. Vent passage; 32. Steam connector pipe; 4. Sampling pipe; 5. Steam sterilization mechanism; 51. Vent valve body; 511. Second gas chamber; 5111. Vent hole; 52. Pressure relief safety chamber; 53. Airtight piston; 531. Connecting rod end; 532. First plug; 533. Second plug; 54. Airtight spring; 6. Isolation sampling mechanism; 61. Sterilization sleeve; 611. Sterilization chamber; 62. Diverter pipe; 63. Isolation chamber; 631. Isolation cavity; 6311. Sampling hole; 632. Isolation sphere; 633. Gas collection hopper; 634. Ball cap; 635. Steam passage; 64. Sampling connector pipe. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Reference Figures 1-7 An embodiment of the present invention provides a sampling mechanism for a bioreactor, comprising: The material chamber 2, used for placing materials, is located in the tank body 1 of the bioreactor (hereinafter referred to as the reactor tank body); In addition, the valve body connector 3 and the sampling tube 4 are fixedly installed at the top of the reaction vessel body 1.

[0024] Furthermore, the end of the sampling tube 4 furthest from the valve body connector 3 extends into the material chamber 2. The valve body connector 3 is also equipped with a steam sterilization mechanism 5 for steam sterilization and a sampling isolation mechanism 6 for sampling. The sampling isolation mechanism 6 includes a sterilization sleeve 61 fixedly installed on the valve body connector 3 and located outside the reaction tank 1. A sterilization chamber 611 is opened in the sterilization sleeve 61. A diversion pipe 62 is fixedly installed at the bottom end of the sterilization sleeve 61, and the sterilization chamber 611 is connected to the sampling tube 4 through the diversion pipe 62. A partition chamber 63 distributed along the axial direction of the sterilization sleeve 61 is also fixedly installed in the sterilization chamber 611. The partition chamber 63 has a partition cavity 631 connected to the diversion pipe 62. A partition ball 632 for sealing the diversion pipe 62 is also slidably connected in the partition chamber 63. A sampling connector pipe 64 connected to the sterilization cavity 61 is fixedly installed at one end of the sterilization sleeve 61 protruding from the reaction vessel body 1. The reaction vessel body 1 is the core load-bearing structure of the biological reaction, with flanged outlet and inlet ports, as well as related monitoring valves. The material chamber 2 provides space for microbial or cellular reactions, ensuring the required sealed environment for the reaction, and can be accelerated by the stirring device built into the reaction vessel body 1. The reaction proceeds, and valve body connector 3 provides an installation base for the installation and fixation of sampling tube 4, steam sterilization mechanism 5, and isolation sampling mechanism 6, ensuring the sealing of each component connection. Sampling tube 4 extends into material chamber 2, directly obtaining gas samples from the core reaction area inside the tank. It is the only component in this device structure directly connected to material chamber 2. Steam sterilization mechanism 5 can use external steam sterilization equipment to perform high-temperature steam sterilization on the gas flow pipeline and core components, and has a certain degree of safety pressure relief function. Isolation sampling mechanism 6 is the sampling core, and its isolation design can solve backflow problems. To address the issue of steam backflow, the sterilization chamber 611 of the sterilization sleeve 61 provides space for steam sterilization. The diversion pipe 62 connects the sampling pipe 4 to the sterilization chamber 611 and provides a channel for gas flow. The partition chamber 63 and the partition ball 632 form an airflow isolation structure. The sampling airflow channel can be opened, closed, and sealed by the gravity sliding of the partition ball 632 to avoid backflow and steam backflow. The sampling connector pipe 64 can be connected to external sampling equipment through its own flange to provide an interface for sampling operations and ensure the airtightness of the sampling process. It is similar in structure and function to the traditional sampling valve and is equipped with corresponding on / off valves.

[0025] The partition chamber 63, near the diversion pipe 62, has an integrally formed gas-gathering hopper 633. The gas-gathering hopper 633 is funnel-shaped and interference-fitted with the partition sphere 632. At the end of the partition chamber 63 away from the gas-gathering hopper 633, on the inner wall of the sterilization sleeve 61, a ball cap 634 is fixedly installed. The ball cap 634 has an arc-shaped surface adapted to the partition sphere 632. The gas-gathering hopper 633 can collect and disperse the gas delivered by the diversion pipe 62, improving gas flow efficiency and allowing the partition sphere 632 to... The sphere 632 slides smoothly and blocks the sliding of the isolation sphere 632, and achieves sealing of the diversion tube 62 in the non-sampling state to prevent backflow of external air or steam from entering. The arc-shaped surface of the sphere cap 634 provides a stable support surface for the isolation sphere 632, which can ensure the stability of the isolation sphere 632 during sampling. An electromagnet can be provided. The attraction force generated by the electromagnet when it is energized can transform the original adaptive gravity sealing of the isolation sphere 632 into a controllable sealing, further adapting to different usage requirements of sampling work.

[0026] The partition chamber 63 is also provided with a sampling hole 6311. The sampling hole 6311 and the ball cap 634 are arranged to avoid each other. The partition chamber 631 is connected to the sterilization chamber 611 through the sampling hole 6311. The sterilization sleeve 61 is also fixedly installed with a steam channel 635 connected to the partition chamber 631. The sampling hole 6311 should avoid conflict with the sealing structure of the ball cap 634 and the partition ball 632 to ensure that the gas can flow smoothly from the partition chamber 631 into the sterilization chamber 611 through the sampling hole 6311. Finally, the sampling is completed through the sampling connector pipe 64. The steam channel 635 provides a channel for steam sterilization, allowing high-temperature steam to enter the partition chamber 631 to sterilize the inside of the partition chamber 63, the sampling hole 6311 and other key parts, eliminating sterilization dead spots. At the same time, with the help of the sealing of the partition ball 632, steam is prevented from flowing back to the reaction tank through the diversion pipe 62.

[0027] The valve body connector 3 has a first air chamber 31 connected to the sampling tube 4. A steam connector pipe 32 for connecting to the steam sterilization equipment is also fixedly installed on the valve body connector 3. One end of the steam connector pipe 32 passes through the valve body connector 3 and is located in the first air chamber 31. The first air chamber 31 provides a transition channel for steam and overflow gas in the sampling tube 4, realizing the connection between steam and overflow gas flow channel. The sampling tube 4 can be completely isolated by blocking the first air chamber 31. The steam connector pipe 32 serves as the connection interface of the external steam sterilization equipment, which can introduce high-temperature steam into the first air chamber 31 to provide a steam source for the subsequent sterilization of the steam sterilization mechanism 5 and the isolation sampling mechanism 6, ensuring the sterility of the entire air flow pipeline.

[0028] The first air chamber 31 is connected to the partition chamber 631 through the steam channel 635. The steam channel 635 extends from one end of the sterilization chamber 611 into the partition chamber 631. The connection between the first air chamber 31 and the partition chamber 631 through the steam channel 635 allows steam to directly reach the partition chamber 631, sampling port 6311, etc., to achieve all-round sterilization. Even if some gas enters the first air chamber 31 through the steam channel 635, it can be sterilized and discharged by high-temperature steam, or backflow can be prevented by setting a one-way flow control valve in the steam channel 635.

[0029] The steam sterilization mechanism 5 includes a vent valve body 51 fixedly installed at the top of the valve body connector 3. The vent valve body 51 has a second air chamber 511 that is concentrically distributed with the first air chamber 31. The second air chamber 511 is connected to the first air chamber 31 through a venting channel 311. Excess overflow gas or sterilization steam from the first air chamber 31 can be transferred to the second air chamber 511 through the venting channel 311, thereby avoiding excessive pressure that could damage the equipment or affect the sealing effect.

[0030] A pressure relief safety chamber 52 is also fixedly installed outside the vent valve body 51. The pressure relief safety chamber 52 has vent holes 5111 connected to the second air chamber 511 distributed in a ring array on it. The diameter of the second air chamber 511 gradually increases from the outside to the inside. When the second air chamber 511 is not blocked, steam can be discharged evenly through the vent holes 5111 to prevent local pressure concentration. The gradually changing diameter of the second air chamber 511 can effectively reduce the resistance when steam is discharged, improve the pressure relief efficiency, and at the same time avoid the generation of negative pressure when steam flows back.

[0031] The vent valve body 51 is also slidably connected to an airtight piston 53 for sealing the sampling tube 4 and the first air chamber 31. An airtight spring 54 for resetting the airtight piston 53 is provided in the vent valve body 51 and on the outer sleeve of the airtight piston 53. The airtight piston 53 can open and close the sampling channel by sliding. The airtight spring 54 can provide a reset elastic force for the airtight piston 53 by its own extension, ensuring the sealing reliability in the non-sampling state. In order to prevent steam corrosion, the surface is reinforced by spray painting.

[0032] The airtight piston 53 has vertically distributed connecting rod ends 531 along its axial direction. The top end of the connecting rod end 531 extends to the outside of the vent valve body 51. A first plug 532 for sealing the first air chamber 31 and a second plug 533 for sealing the second air chamber 511 are fixedly installed at both ends of the connecting rod end 531, respectively. The first plug 532 extends into the sampling tube 4 at a tapered end, and the second plug 533 is interference-fitted with the pressure relief safety chamber 52. The connecting rod end 531 connects the first plug 532 and the second plug 533 to achieve synchronous action, thereby reducing pressure inside the tank. When the force exceeds the rated value of the airtight spring 54, the gas pressure inside the tank or the external sampling driving force pushes the airtight piston 53 upward. The first plug 532 disengages from the sampling tube 4, and the second plug 533 disengages from the second gas chamber 511. The gas can flow smoothly, and after the excess gas is released, the airtight spring 54 returns to its original position. The two plugs block the corresponding channels respectively. The conical design of the first plug 532 can enhance the sealing effect with the sampling tube 4. The second plug 533 is interference-fitted with the pressure relief safety chamber 52 to ensure sealing in non-pressure relief state and prevent unsterilized air from entering the gas path.

[0033] The two ends of the airtight spring 54 are respectively connected to the inner wall of the vent valve body 51 and the second plug 533. The airtight spring 54 can ensure that the spring applies a stable preload to the second plug 533, so that the first plug 532 is always tightly attached to the port of the sampling tube 4 and the second plug 533 is tightly attached to the sealing surface of the second air chamber 511. Moreover, the airtight spring 54 does not immediately depressurize when it undergoes slight elastic deformation. As long as the second plug 533 is not completely inserted into the depressurization safety chamber 52, the entire air passage is still in a closed state.

[0034] During the sampling process of the bioreactor using the sampling mechanism of this embodiment, an external sampling device is connected via the flange on the sampling connector pipe 64. After the connection is stable, the on / off valve on the connector pipe is opened. The sample gas in the material chamber 2 of the reactor body 1 flows into the diversion pipe 62 through the sampling pipe 4. After being gathered by the gas gathering bucket 633, it pushes the partition ball 632 to slide along the partition cavity 631 to the arc surface of the ball cap 634. At this time, the diversion pipe 62 is connected to the partition cavity 631, and the gas passes through the sampling hole designed with avoidance through the partition cavity 631. 6311 flows into the sterilization chamber 611 and finally completes the sealed sampling through the sampling connector tube 64. After sampling, the pressure inside the tank decreases, and the partition ball 632 falls back to the gas gathering hopper 633, sealing the connection between the diversion tube 62 and the partition chamber 631, thereby preventing external impurities from entering the reaction tank body 1 through the unclosed sampling connector tube 64. If it is necessary to adjust the sampling rate, an electromagnet that can attract the partition ball 632 can be set in the ball cap 634. The sliding position of the partition ball 632 can be controlled by the attraction force generated by the electromagnet being energized, so as to achieve controllable airflow rate.

[0035] When the pressure in the reaction vessel 1 exceeds the rated preload of the airtight spring 54, it pushes the airtight piston 53 upward. Simultaneously, the connecting rod end 531 drives the first plug 532 to disengage from the sampling tube 4 and the second plug 533 to disengage from the second gas chamber 511. Only when the second plug 533 is fully inserted into the pressure relief safety chamber 52 can the airflow channel be unlocked. Excess steam and the pressure generated by sterilization flow into the second gas chamber 511 through the venting channel 311 connected to the first gas chamber 31. The gas is then evenly discharged using the connected second gas chamber 511 and the annular venting holes 5111 with varying apertures on the pressure relief safety chamber 52, preventing excessive gas pressure from damaging the equipment. This assists the safety pressure relief structure built into the reaction vessel 1 for protection, further enhancing the safety of the device.

[0036] During steam sterilization, the airtight spring 54 provides a stable pre-tightening force, pushing the first plug 532 of the airtight piston 53 to tightly fit the port of the sampling tube 4, sealing the connection between the sampling tube and the first air chamber 31. At the same time, the second plug 533 is interference-fitted with the pressure relief safety chamber 52, sealing the second air chamber 511, forming the first sealing barrier. The partition ball 632 in the partition chamber 63 relies on its own gravity and is interference-fitted with the air gathering bucket 633 to seal the connection between the diversion pipe 62 and the partition chamber 631, forming a double sealing structure. The external steam sterilization equipment introduces high-temperature steam into the first air chamber 31 through the steam connector pipe 32. The steam diffuses in two directions: one direction goes directly to the partition chamber 631 through the steam channel 635 to sterilize the core components such as the partition chamber 63, the sampling hole 6311, and the partition ball 632; the other direction flows through the sterilization chamber 611 to sterilize the sterilization sleeve 61, the sampling connector pipe 64, and other pipelines, achieving coverage without dead angles.

[0037] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A sampling mechanism for a bioreactor vessel, characterized by, include: The material chamber (2) for placing materials is located in the tank body (1) of the reaction vessel; The valve body connector (3) and the sampling tube (4) are fixedly installed at the top of the reaction tank body (1). The end of the sampling tube (4) away from the valve body connector (3) extends into the material chamber (2). The valve body connector (3) is also provided with a steam sterilization mechanism (5) for steam sterilization and a sampling isolation mechanism (6) for sampling. The isolation sampling mechanism (6) includes a sterilization sleeve (61) fixedly installed on the valve body connector (3) and located outside the reaction vessel body (1). The sterilization sleeve (61) has a sterilization chamber (611). A diversion pipe (62) is fixedly installed at the bottom end of the sterilization sleeve (61), and the sterilization chamber (611) is connected to the sampling pipe (4) through the diversion pipe (62). The sterilization chamber (611) is also fixedly installed with... There is a partition chamber (63) distributed along the axial direction of the sterilization sleeve (61). The partition chamber (63) is provided with a partition cavity (631) connected to the diversion pipe (62). The partition chamber (63) is also slidably connected with a partition ball (632) for sealing the diversion pipe (62). The end of the sterilization sleeve (61) protruding outside the reaction tank body (1) is also fixedly installed with a sampling connector pipe (64) connected to the sterilization cavity (611).

2. The sampling mechanism for a bioreactor vessel of claim 1, wherein, The partition chamber (63) is provided with an integrally formed air-gathering hopper (633) at one end near the diversion pipe (62). The air-gathering hopper (633) is funnel-shaped and is connected to the partition sphere (632) with an interference fit. A ball cap (634) is fixedly installed on the inner wall of the sterilization sleeve (61) at the end of the partition chamber (63) away from the air-gathering hopper (633). The ball cap (634) has an arc-shaped surface that is compatible with the partition sphere (632).

3. The sampling mechanism for a bioreactor according to claim 2, characterized in that, The partition chamber (63) is also provided with a sampling hole (6311), which is arranged to avoid the ball cap (634). The partition chamber (631) is connected to the sterilization chamber (611) through the sampling hole (6311). The sterilization sleeve (61) is also fixedly installed with a steam channel (635) connected to the partition chamber (631).

4. The sampling mechanism for a bioreactor according to claim 1, characterized in that, The valve body connector (3) has a first air chamber (31) connected to the sampling tube (4). A steam connector pipe (32) for connecting to the steam sterilization equipment is also fixedly installed on the valve body connector (3). One end of the steam connector pipe (32) passes through the valve body connector (3) and is located in the first air chamber (31).

5. The sampling mechanism for a bioreactor vessel of claim 4, wherein, The first air chamber (31) is connected to the partition chamber (631) through a steam channel (635), which extends from one end of the steam channel (635) through the sterilization chamber (611) into the partition chamber (631).

6. The sampling mechanism for a bioreactor vessel of claim 1, wherein, The steam sterilization mechanism (5) includes a vent valve body (51) fixedly installed at the top of the valve body connector (3). The vent valve body (51) has a second air chamber (511) that is concentrically distributed with the first air chamber (31). The second air chamber (511) is connected to the first air chamber (31) through the vent channel (311).

7. The sampling mechanism for a bioreactor vessel of claim 6, wherein, The pressure relief valve body (51) is also fixedly installed with a pressure relief safety chamber (52). The pressure relief safety chamber (52) has a ring array of vent holes (5111) that communicate with the second air chamber (511), and the diameter of the second air chamber (511) gradually increases from the outside to the inside.

8. The sampling mechanism for a bioreactor vessel of claim 7, wherein, The vent valve body (51) is also slidably connected to an airtight piston (53) for sealing the sampling tube (4) and the first air chamber (31). An airtight spring (54) for resetting the airtight piston (53) is provided in the vent valve body (51) and outside the airtight piston (53).

9. The sampling mechanism for a bioreactor vessel of claim 8, wherein, The airtight piston (53) has vertically distributed connecting rod ends (531) along its axial direction. The top end of the connecting rod end (531) extends to the outside of the vent valve body (51). The two ends of the connecting rod end (531) are respectively fixedly installed with a first plug (532) for sealing the first air chamber (31) and a second plug (533) for sealing the second air chamber (511). The first plug (532) extends into the sampling tube (4) at one end in a conical shape, and the second plug (533) is connected to the depressurization safety chamber (52) with an interference fit.

10. The sampling mechanism for a bioreactor vessel of claim 8, wherein, The two ends of the airtight spring (54) are respectively connected to the inner wall of the vent valve body (51) and the second plug (533).

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