Dental pulp stem cell exosome sustained-release microsphere tooth regeneration composition and periodontal repair method

Abstract

This invention belongs to the field of oral regenerative medicine and biomedical materials technology, particularly a tooth regeneration composition for dental pulp stem cell exosome sustained-release microspheres. Its characteristics include: a composition of dental pulp stem cell exosomes, a gelatin-alginic acid composite microsphere carrier, hyaluronic acid, chitosan, and medical saline; the purity of the dental pulp stem cell exosomes is ≥98%, and they are encapsulated within the gelatin-alginic acid composite microspheres, with an exosome encapsulation rate ≥90%; the mass ratio of gelatin to alginate in the gelatin-alginic acid composite microspheres is 2:1, and the microsphere particle size is 50-200 μm; the pH value of the composition is 6.8-7.2. This invention provides integrated synergistic regeneration: breaking through the limitations of single-tissue repair, it simultaneously achieves enamel and dentin repair, pulp tissue regeneration, periodontal ligament reconstruction, and alveolar bone maintenance, realizing comprehensive tooth and periodontal repair with a tooth repair success rate ≥90%, a periodontal symptom improvement rate ≥85%, and a loose tooth retention rate ≥88%.

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