A Ganoderma lucidum cell wall broken spore powder and its preparation method
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- ZHEJIANG MEDICAL COLLEGE
- Filing Date
- 2026-04-23
- Publication Date
- 2026-06-30
Smart Images
Figure SMS_1
Abstract
Description
Technical Field
[0001] This invention relates to the field of traditional Chinese medicine processing technology, and in particular to a Ganoderma lucidum cell wall broken spore powder and its preparation method. Background Technology
[0002] Reishi spores are extremely tiny, oval-shaped reproductive cells ejected from the gills of the reishi mushroom during its growth and maturation period; they are essentially the seeds of the reishi. Each reishi spore is only 4-6 micrometers in size, a living organism with a double-walled structure. Reishi spores contain all the genetic material and health benefits of the reishi mushroom. Its medicinal value is increasingly recognized; research has found that reishi spores can enhance immunity, inhibit tumors, protect against liver damage, and provide radiation protection. Reishi spores have two spore walls (polysaccharide walls) composed of chitin and glucan, with a concentric network structure. They are tough, resistant to acids and alkalis, and extremely difficult to oxidize and decompose, thus limiting the digestion and absorption of the effective substances within the spores. To fully utilize the effective substances within the reishi spores, the spore walls must be broken to facilitate their utilization. Scientific experiments have confirmed that only 10-20% of the effective components of undisturbed spores are absorbed by the human body, while the absorption rate of effective components after wall breaking exceeds 90%.
[0003] Currently used cell wall breaking methods, such as chemical and biological methods, are prone to problems such as residues or low cell wall breaking rates, while mechanical methods do not have a fixed process, resulting in inconsistent product quality. Summary of the Invention
[0004] The purpose of this invention is to provide a Ganoderma lucidum spore powder with broken cell walls and its preparation method, which ensures the quality of the product while guaranteeing the cell wall breakage rate.
[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides a method for preparing Ganoderma lucidum spore powder with broken cell walls, comprising the following steps: (1) Ganoderma lucidum spore powder was treated with enzyme and then frozen to obtain material 1; (2) After microwave treatment of material 1, air jet pulverization is performed to obtain Ganoderma lucidum cell wall broken spore powder.
[0006] Preferably, the enzyme treatment method in step (1) is as follows: soaking Ganoderma lucidum spore powder in an enzyme solution for 10-30 minutes and then filtering.
[0007] Preferably, the mass ratio of the Ganoderma lucidum spore powder to the enzyme solution is 1:1~2, and the concentration of the enzyme in the enzyme solution is 4~6 mg / g.
[0008] Preferably, the enzyme in step (1) is one or more of chitinase, cellulase and pectinase.
[0009] Preferably, in step (1), the Ganoderma lucidum spore powder is dried before freezing, and the moisture content of the dried Ganoderma lucidum spore powder is 8-12%.
[0010] Preferably, the freezing temperature in step (1) is -75~-85℃, and the freezing time is 30~60min.
[0011] Preferably, the microwave power in step (2) is 80~120w, and the microwave duration in step (2) is 10~20min.
[0012] Preferably, the air pressure in the air jet milling process in step (2) is 0.8~1.5MPa, and the air jet milling time in step (2) is 15~20min.
[0013] The present invention also provides Ganoderma lucidum cell wall broken spore powder prepared by the preparation method described above.
[0014] This invention provides a Ganoderma lucidum spore powder with broken cell walls and its preparation method. The preparation method includes the following steps: (1) treating Ganoderma lucidum spore powder with enzymes and then freezing it to obtain material 1; (2) treating material 1 with microwaves and then performing airflow pulverization to obtain Ganoderma lucidum spore powder with broken cell walls. The preparation method of this invention can effectively break the outer wall of Ganoderma lucidum spore powder, and the finished product has no chemical residues. The cell wall breaking rate is greater than 99%, which increases the proportion of effective components such as Ganoderma lucidum polysaccharides and Ganoderma lucidum triterpenes in the product, and ensures the quality of the product while ensuring the cell wall breaking rate. Detailed Implementation
[0015] This invention provides a method for preparing Ganoderma lucidum spore powder with broken cell walls, comprising the following steps: (1) Ganoderma lucidum spore powder was treated with enzyme and then frozen to obtain material 1; (2) After microwave treatment of material 1, air jet pulverization is performed to obtain Ganoderma lucidum cell wall broken spore powder.
[0016] In this invention, the air jet mill is preferably used for the air jet milling process.
[0017] In this invention, the enzyme treatment method in step (1) is as follows: soak Ganoderma lucidum spore powder in an enzyme solution for 10-30 minutes and then filter.
[0018] In this invention, the soaking time is preferably 15-25 minutes, and more preferably 20 minutes.
[0019] In this invention, the mass ratio of Ganoderma lucidum spore powder to enzyme solution is preferably 1:1~2, and more preferably 1:1.5.
[0020] In this invention, the concentration of the enzyme in the enzyme solution is preferably 4-6 mg / g, and more preferably 5 mg / g.
[0021] In this invention, the enzyme in step (1) is preferably one or more of chitinase, cellulase and pectinase, and more preferably chitinase.
[0022] In this invention, it is preferable to dry the Ganoderma lucidum spore powder before freezing in step (1).
[0023] In this invention, the moisture content of the dried Ganoderma lucidum spore powder is preferably 8-12%, more preferably 9-11%, and even more preferably 10%.
[0024] In this invention, the freezing temperature in step (1) is preferably -75~-85℃, more preferably -78~-82℃, and even more preferably -80℃.
[0025] In this invention, the freezing time is preferably 30-60 min, more preferably 40-50 min, and even more preferably 45 min.
[0026] In this invention, the power of the microwave in step (2) is preferably 80~120w, more preferably 90~110w, and even more preferably 100w.
[0027] In this invention, the microwave time in step (2) is preferably 10-20 min, more preferably 12-18 min, and even more preferably 15 min.
[0028] In this invention, the air pressure in the airflow pulverization process in step (2) is preferably 0.8~1.5MPa, more preferably 1~1.3MPa, and even more preferably 1.1~1.2MPa.
[0029] In this invention, the airflow pulverization time in step (2) is preferably 15-20 min, more preferably 16-19 min, and even more preferably 17-18 min.
[0030] The present invention also provides Ganoderma lucidum cell wall broken spore powder prepared by the preparation method described above.
[0031] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0032] Example 1
[0033] A method for preparing Ganoderma lucidum spore powder with broken cell wall: (1) Take 10g of Ganoderma lucidum spore powder, soak it in 15g chitinase solution (5mg / g) for 20min, filter it, dry the Ganoderma lucidum spore powder to a moisture content of 10%, freeze it at -80℃ for 45min to obtain material 1; (2) Material 1 was treated in a microwave at 100W for 15 minutes and then treated in an air jet mill at 1.3MPa for 18 minutes to obtain Ganoderma lucidum cell wall broken spore powder.
[0034] Example 2
[0035] A method for preparing Ganoderma lucidum spore powder with broken cell wall: (1) Take 10g of Ganoderma lucidum spore powder, soak it in 10g of cellulase solution (6mg / g) for 25min, filter it, dry the Ganoderma lucidum spore powder to a moisture content of 8%, and freeze it at -85℃ for 30min to obtain material 1; (2) Material 1 was treated in a microwave at 80W for 20 minutes and then treated in an air jet mill at 0.8MPa for 20 minutes to obtain Ganoderma lucidum cell wall broken spore powder.
[0036] Example 3
[0037] A method for preparing Ganoderma lucidum spore powder with broken cell wall: (1) Take 10g of Ganoderma lucidum spore powder, soak it in 20g of pectinase solution (4mg / g) for 15min, filter it, dry the Ganoderma lucidum spore powder to a moisture content of 12%, freeze it at -75℃ for 60min to obtain material 1; (2) Material 1 was treated in a microwave at 120W for 10 minutes and then treated in an air jet mill at 1.5MPa for 15 minutes to obtain Ganoderma lucidum cell wall broken spore powder.
[0038] Test case
[0039] 40g of Ganoderma lucidum spore powder was divided into 4 equal parts of 10g each, numbered as control group and experimental groups 1-3. Experimental groups 1-3 were prepared using the methods of Examples 1-3, respectively, while the control group was not treated. The cell wall breakage rate, Ganoderma lucidum polysaccharide content and Ganoderma lucidum triterpenoid content of each group were detected. The results are shown in Table 1.
[0040] Table 1
[0041] Conclusion: As can be seen from the results in Table 1, the preparation method of the present invention has a high cell wall breakage rate, which can effectively release the effective components of Ganoderma lucidum while avoiding the loss of effective components, thus ensuring the quality of the product while ensuring the cell wall breakage rate.
[0042] As can be seen from the above embodiments, the present invention provides a Ganoderma lucidum spore powder with broken cell walls and its preparation method. The preparation method includes the following steps: (1) treating Ganoderma lucidum spore powder with enzymes and then freezing it to obtain material 1; (2) treating material 1 with microwaves and then performing airflow pulverization to obtain Ganoderma lucidum spore powder with broken cell walls. The preparation method of the present invention can effectively break the outer wall of Ganoderma lucidum spore powder, and the finished product has no chemical residues. The cell wall breaking rate is greater than 99%, which increases the proportion of effective components such as Ganoderma lucidum polysaccharides and Ganoderma lucidum triterpenes in the product, and ensures the quality of the product while ensuring the cell wall breaking rate.
[0043] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A method for preparing Ganoderma lucidum spore powder, characterized in that, Includes the following steps: (1) Ganoderma lucidum spore powder was treated with enzyme and then frozen to obtain material 1; (2) After microwave treatment of material 1, air jet pulverization is performed to obtain Ganoderma lucidum cell wall broken spore powder.
2. The production method according to claim 1, characterized by, The enzyme treatment method in step (1) is as follows: soak Ganoderma lucidum spore powder in an enzyme solution for 10-30 minutes and then filter.
3. The preparation method according to claim 2, characterized in that, The mass ratio of Ganoderma lucidum spore powder to enzyme solution is 1:1~2, and the concentration of enzyme in the enzyme solution is 4~6 mg / g.
4. The preparation method according to claim 3, characterized in that, The enzyme in step (1) is one or more of chitinase, cellulase and pectinase.
5. The preparation method according to claim 4, characterized in that, In step (1), the Ganoderma lucidum spore powder is dried before freezing, and the moisture content of the dried Ganoderma lucidum spore powder is 8-12%.
6. The preparation method according to claim 5, characterized in that, The freezing temperature in step (1) is -75~-85℃, and the freezing time is 30~60min.
7. The preparation method according to claim 6, characterized in that, The microwave power in step (2) is 80~120w, and the microwave duration in step (2) is 10~20min.
8. The preparation method according to any one of claims 1 to 7, characterized in that, The air pressure in the airflow pulverization process in step (2) is 0.8~1.5MPa, and the airflow pulverization time in step (2) is 15~20min.
9. Ganoderma lucidum spore powder prepared by the preparation method according to any one of claims 1 to 8.