A jujube extract with sleep-improving function and its preparation method
By integrating processes and using precise ingredient ratios, the sleep-improving effects of jujube extract have been resolved, achieving highly efficient and stable sleep improvement results, making it suitable for both pharmaceuticals and functional foods.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- RUOQIANG HONGJIANGQIANGZAO TECHNOLOGY CO LTD
- Filing Date
- 2026-04-29
- Publication Date
- 2026-06-30
AI Technical Summary
Existing jujube extracts have inconsistent effects on improving sleep function. Traditional extraction methods are inefficient, the components are easily degraded, and it is difficult to achieve high-activity and standardized production.
An integrated process of compound enzymatic hydrolysis-dynamic countercurrent extraction-gradient alcohol precipitation-simulated moving bed chromatography was adopted to define the precise ratio of jujube polysaccharides, total flavonoids and total triterpenic acids. γ-aminobutyric acid and 5-hydroxytryptamine were added, and efficient separation was achieved through simulated moving bed chromatography to form a jujube extract.
It significantly shortens sleep latency and prolongs sleep time at low doses, with effects far exceeding those of single components or simple mixtures. It is suitable for industrial production and has good product stability and reproducibility.
Smart Images
Figure CN122297574A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of natural product extraction and functional food technology, specifically to a jujube extract with sleep-improving function and its preparation method. Background Technology
[0002] Sleep is an essential physiological process for the human body, and good sleep is crucial for maintaining physical and mental health. With the accelerated pace of modern life and increased work pressure, the incidence of sleep disorders (such as difficulty falling asleep, difficulty maintaining sleep, and early awakening) is rising year by year, becoming a global public health problem. Currently, commonly used medications for treating insomnia mainly include benzodiazepine receptor agonists (such as diazepam and estazolam) and non-benzodiazepines (such as zolpidem). While these drugs can improve sleep in the short term, long-term use can easily lead to dependence, tolerance, withdrawal symptoms, and adverse reactions such as daytime sleepiness and cognitive impairment. Therefore, developing safe, effective, and low-side-effect-prone sleep-improving products from natural sources is of great significance.
[0003] Red dates (Ziziphus jujuba Mill.), also known as jujubes, are a traditional Chinese medicinal and edible resource, known for their effects of tonifying the middle energizer, nourishing blood, and calming the mind. Traditional Chinese medicine believes that red dates can "calm the mind and nourish the spirit," and are often used to treat symptoms such as insomnia due to deficiency and restlessness, and palpitations. Modern research shows that red dates contain various active ingredients, such as polysaccharides, flavonoids, triterpenoids, and nucleotides, which possess various biological activities including antioxidant, immunomodulatory, and neuroprotective effects. However, existing research on the sleep-improving function of red dates mostly focuses on simple crude extracts with complex compositions and unclear mechanisms of action. Furthermore, the active ingredients vary greatly depending on the origin, variety, and extraction process, leading to unstable and poor reproducibility of their sleep-improving effects. In addition, traditional extraction methods (such as water extraction and alcohol precipitation, reflux extraction, etc.) suffer from low extraction rates, easy degradation of active ingredients, and low product purity, making it difficult to produce highly active and standardized extracts.
[0004] Therefore, this invention proposes a jujube extract with sleep-improving function and its preparation method to solve the above problems. Summary of the Invention
[0005] To overcome the shortcomings of the prior art, the present invention aims to provide a jujube extract with sleep-improving function and its preparation method.
[0006] To achieve the aforementioned objective, the technical solution of the present invention is as follows: a jujube extract with sleep-improving function.
[0007] The extract contains jujube polysaccharides, total jujube flavonoids, and total jujube triterpenoids in a weight ratio of 1:(0.3~0.6):(0.1~0.2).
[0008] The molecular weight distribution of the jujube polysaccharide is as follows: the proportion of components with a weight-average molecular weight (Mw) in the range of 10~50kDa is ≥65% (i.e., low molecular weight polysaccharides are dominant). Furthermore, in the polysaccharide components within this molecular weight range, the molar ratio of glucose, galactose, and arabinose in their monosaccharide composition is (2~4):(1~2):(1~1.5).
[0009] The total flavonoids from the jujube contain at least 0.5 wt% of spinosin and 0.3 wt% of 6'''-feruloylspinosin.
[0010] In animal experiments, the jujube extract, administered by gavage at a dose of 100-300 mg / kg, shortened the sleep latency of sodium pentobarbital-induced mice by ≥40% and prolonged the sleep time by ≥80%.
[0011] As a preferred embodiment, the extract also contains γ-aminobutyric acid (GABA) and serotonin (5-HT), with a weight ratio of GABA to serotonin of 1:(0.02~0.05). The addition of these two neurotransmitters can further synergistically enhance its sleep-improving effect.
[0012] Furthermore, the present invention provides a method for preparing the above-mentioned jujube extract with sleep-improving function.
[0013] The method includes the following steps: (1) Compound enzymatic hydrolysis pretreatment: Pit and crush the jujubes to 20-60 mesh. Add 3-8 times their weight of water, adjust the pH to 4.0-5.5, add a compound enzyme, and enzymatically hydrolyze at 40-55℃ for 1-3 hours to obtain the enzymatic hydrolysate. The compound enzyme consists of cellulase, pectinase, and xylanase in a weight ratio of (2-3):(1-2):(0.5-1), with the total enzyme addition amounting to 0.5%-2% of the dry weight of the jujubes. This step breaks down the plant cell walls through enzymatic hydrolysis, promoting the release of intracellular active ingredients.
[0014] (2) Dynamic countercurrent extraction: The enzymatic hydrolysate obtained in step (1) is heated to 80-95℃ and continuously extracted in a dynamic countercurrent extraction device. The liquid-to-solid ratio of the extract to the material is (10-20):1, and the extraction time is 30-60 minutes. The extract is collected, and the extraction residue is separated at the same time. Dynamic countercurrent extraction can maintain a large concentration gradient, improve extraction efficiency and rate, and shorten extraction time.
[0015] (3) Gradient alcohol precipitation fractionation: The extract obtained in step (2) was concentrated to 1 / 5 to 1 / 3 of its original volume. Ethanol was first added until the ethanol volume fraction reached 40% to 50%, and the mixture was allowed to stand and precipitate. The first precipitate (polysaccharide component A) was collected by centrifugation. Ethanol was then added to the supernatant until the ethanol volume fraction reached 75% to 85%, and the mixture was allowed to stand and precipitate. The second precipitate (polysaccharide component B) was collected by centrifugation. Polysaccharide components A and B were combined to obtain the jujube polysaccharide. This gradient alcohol precipitation step achieved selective precipitation of polysaccharides with different molecular weights, enriching polysaccharides within the target molecular weight range (10-50 kDa).
[0016] (4) Simulated moving bed chromatography (SMBC) purification: The final supernatant after the two alcohol precipitations in step (3) was concentrated under reduced pressure to recover ethanol, and then separated using a simulated moving bed chromatography system. The stationary phase was C18 or phenyl-bonded silica gel, and the mobile phase was an ethanol-water-acetic acid system. The total flavonoid fraction and the total triterpene acid fraction were collected according to their retention times. SMBC is a continuous chromatography technique that can achieve efficient, large-scale, and low-cost separation to obtain high-purity total flavonoids and total triterpene acids.
[0017] (5) Targeted compounding: The jujube polysaccharide obtained in step (3), the total jujube flavonoids obtained in step (4), and the total jujube triterpenoids are compounded and mixed evenly according to the weight ratio (1:0.3~0.6:0.1~0.2) described in claim 1, and then dried to obtain the final product.
[0018] As a preferred embodiment, before the compound enzymatic hydrolysis in step (1), a low-frequency ultrasonic-assisted treatment step is also included: ultrasonic treatment for 10 to 20 minutes at 20 to 40 kHz and a power density of 50 to 200 W / L to further destroy the cell wall structure of jujube and improve the efficiency of subsequent enzymatic hydrolysis.
[0019] As a preferred option, the dynamic countercurrent extraction device in step (2) is a multi-stage spiral countercurrent extractor. During the extraction process, the linear velocity of the extract is maintained at 0.5~2cm / s, and nitrogen is continuously introduced during the extraction process to isolate oxygen and avoid oxidation of active ingredients such as flavonoids and triterpenic acids.
[0020] As a preferred embodiment, the operating parameters of the simulated moving bed chromatography system in step (4) are as follows: injection flow rate 1~5 mL / min, elution flow rate 20~50 mL / min, switching time 5~15 minutes, column temperature 25~40℃, and ethanol-water-acetic acid with a volume ratio of (15~30):(70~85):(0.5~2) as the mobile phase. The first fraction (total flavonoids) with a retention time of 0.8~1.5 times the switching time and the second fraction (total triterpenic acids) with a retention time of 2.0~3.5 times the switching time are collected respectively.
[0021] As a preferred option, the drying in step (5) is vacuum belt drying or low-temperature spray drying, with a drying temperature ≤60°C, in order to obtain an extract powder with good flowability and solubility, and to avoid the degradation of heat-sensitive components (such as polysaccharides and flavonoids).
[0022] Furthermore, the present invention provides the application of the above-mentioned jujube extract in the preparation of drugs, health foods or functional foods with sleep-improving functions.
[0023] The aforementioned medicine, health food, or functional food may contain a therapeutically or health-promoting amount of the jujube extract described in this invention, as well as one or more pharmaceutically or food-grade excipients, such as fillers, binders, disintegrants, lubricants, flavoring agents, etc. Its dosage form may be tablets, capsules, granules, oral liquids, powders, etc.
[0024] The beneficial effects of this invention are reflected in: This invention, for the first time, defines the precise proportions of the core active ingredient groups (polysaccharides, total flavonoids, and total triterpenic acids) in a jujube sleep-improving extract, and specifies clear requirements for the molecular weight distribution of polysaccharides, monosaccharide composition, and the content of two key markers in flavonoids (spinosin and 6'''-feruloylspinosin). This provides a scientific basis for product standardization and quality control, ensuring the stability and reproducibility of efficacy. The extract obtained through targeted compounding exhibits a synergistic effect among its three components, demonstrating effects far exceeding those of single components or simple mixtures in animal experiments. At low doses of 100-300 mg / kg, it can shorten sleep latency by more than 40% and prolong sleep time by more than 80%, significantly outperforming traditional jujube extracts. This invention employs an integrated process of "compound enzymatic hydrolysis-dynamic countercurrent extraction-gradient alcohol precipitation-simulated moving bed chromatography". The combination of enzymatic hydrolysis and ultrasonic pretreatment significantly improves the release and extraction rates of active ingredients; dynamic countercurrent extraction shortens the extraction time and reduces energy consumption; gradient alcohol precipitation achieves the enrichment of target polysaccharides; and simulated moving bed chromatography enables efficient and continuous separation of total flavonoids and total triterpenic acids. The overall process is green and efficient, suitable for industrial production; the entire extraction and drying process (such as nitrogen protection and low-temperature drying) fully considers the stability of active ingredients, avoiding oxidation and thermal degradation, and the resulting product has good flowability and solubility, facilitating subsequent formulation processing. Attached Figure Description
[0025] In the attached diagram: Figure 1 This is a schematic diagram of the preparation process of the present invention; Figure 2 The molecular weight distribution of jujube polysaccharide prepared in Example 1 is shown by gel permeation chromatography (GPC).
[0026] Figure 3The image shows the high-performance liquid chromatography (HPLC) chromatogram of total flavonoids from jujube prepared in Example 1, with the peak positions of spinosin and 6'''-feruloyl spinosin marked. Detailed Implementation
[0027] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. Obviously, the described embodiments are only a part of the embodiments of the invention, and not all of them. Unless otherwise specified, the embodiments and features described in this application can be combined with each other. All other embodiments obtained by those skilled in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention.
[0028] It should be noted that if the embodiments of the invention involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.
[0029] Furthermore, "multiple" refers to two or more. Additionally, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of a person skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by the invention. Example
[0030] Preparation of Jujube Extract (1) Pretreatment and ultrasonic assistance: Take 1 kg of Ruoqiang red dates from Xinjiang, remove the pits and crush them to 40 mesh. Add 5 L of water and adjust the pH to 5.0. Ultrasonically treat for 15 minutes at 30 kHz and a power density of 100 W / L. Then add a compound enzyme (cellulase: pectinase: xylanase = 2.5:1.5:0.8, with the total enzyme amount accounting for 1.2% of the dry weight of the red dates) and enzymatically hydrolyze at 50℃ for 2 hours.
[0031] (2) Dynamic countercurrent extraction: The enzymatic hydrolysate was heated to 90°C and fed into a multi-stage spiral countercurrent extractor. The liquid-to-solid ratio was controlled at 15:1, the linear velocity of the extract was 1 cm / s, and nitrogen gas was continuously introduced. After extraction for 45 minutes, the extract was collected and the residue was discharged.
[0032] (3) Gradient ethanol precipitation: The extract was concentrated under reduced pressure to 1 / 4 of its original volume. 95% ethanol was added with stirring until the ethanol concentration of the system reached 45%. The mixture was allowed to stand overnight at 4°C, and the precipitate (polysaccharide A) was collected by centrifugation. Ethanol was added to the supernatant until the ethanol concentration reached 80%. The mixture was allowed to stand overnight at 4°C, and the precipitate (polysaccharide B) was collected by centrifugation. Polysaccharides A and B were combined to obtain 28.5 g of jujube polysaccharide.
[0033] (4) SMBC purification: After concentrating the final supernatant from step (3) under reduced pressure to recover ethanol, the supernatant was purified by SMBC. The stationary phase was C18 silica gel, and the mobile phase was ethanol:water:acetic acid = 20:78:2 (volume ratio). Operating parameters: injection flow rate 2 mL / min, elution flow rate 30 mL / min, switching time 10 min, column temperature 30℃. The fraction with a retention time of 8-12 min (0.8-1.2 times the switching time) was collected as the total flavonoid fraction, and the fraction with a retention time of 20-30 min (2.0-3.0 times the switching time) was collected as the total triterpenic acid fraction. The fractions were concentrated under reduced pressure and dried to obtain 4.8 g of total flavonoids and 2.1 g of total triterpenic acids from jujube.
[0034] (5) Targeted compounding: Take 20 g of the above-mentioned jujube polysaccharide, 8 g of total jujube flavonoids (corresponding weight ratio 0.4), and 3 g of total jujube triterpenic acids (corresponding weight ratio 0.15), and mix them evenly. Dry them in a vacuum belt at 60℃, pulverize and sieve to obtain 31 g of jujube extract powder.
[0035] Product Characterization Polysaccharide analysis: GPC analysis showed that 72.5% of the obtained jujube polysaccharides had a molecular weight (Mw) of 10–50 kDa. Pre-column derivatization-HPLC analysis using PMP showed that the molar ratio of glucose, galactose, and arabinose in this polysaccharide component was 3.2:1.5:1.2.
[0036] Flavonoid analysis: HPLC analysis showed that the total flavonoids in the jujubes contained 0.62 wt% spinosin and 0.38 wt% 6'''-feruloyl spinosin. Example
[0037] The preparation method is the same as in Example 1, except that the proportions are adjusted during the directional compounding in step (5): Take 20 g of jujube polysaccharide, 6 g of total jujube flavonoids (weight ratio 0.3), and 2 g of total jujube triterpenic acids (weight ratio 0.1), and mix them evenly. The extract is obtained. Example
[0038] The preparation method is the same as in Example 1, except that the proportions are adjusted in step (5) during the directional compounding: take 20 g of jujube polysaccharide, 12 g of total jujube flavonoids (weight ratio 0.6), and 4 g of total jujube triterpenic acids (weight ratio 0.2), and mix them evenly. The extract is obtained.
[0039] Comparative Example 1 (Traditional Water Extract): Take 1 kg of red dates, remove the pits and crush them, add 10 times the amount of water, boil and extract twice, each time for 2 hours, combine the filtrates, concentrate and dry to obtain traditional red date water extract.
[0040] Comparative Example 2 (single component): Take the jujube polysaccharide prepared in Example 1 and use it alone.
[0041] Comparative Example 3 (non-target molecular weight polysaccharide): During the preparation process, only 40% of the polysaccharide before alcohol precipitation in step (3) was collected (mainly high molecular weight polysaccharide, Mw>100kDa), and the remaining steps were the same as in Example 1.
[0042] Example of effect: Animal experiment on improved sleep function Laboratory animals: SPF-grade Kunming mice, half male and half female, weighing 18-22g, provided by Beijing Vital River Laboratory Animal Technology Co., Ltd.
[0043] Grouping and administration: Mice were randomly divided into a model control group (administered an equal volume of physiological saline), a positive control group (diazepam, 1 mg / kg), Example 1-3 groups (all at a dose of 200 mg / kg), and Comparative Example 1-3 groups (all at a dose of 200 mg / kg). Each group consisted of 12 mice. Administration was performed by gavage once daily for 7 consecutive days.
[0044] Experimental Methods: One hour after the last administration, all mice except the control group were intraperitoneally injected with sodium pentobarbital (45 mg / kg). The disappearance of the righting reflex was used as the indicator of sleep onset. The time from injection to the disappearance of the righting reflex (sleep latency) and the time from the disappearance to the recovery of the righting reflex (sleep duration) were recorded. Results are expressed as mean ± standard deviation, and t-tests were used to compare differences between groups.
[0045] Experimental results are shown in the table below.
[0046] Group Sleep latency (minutes) Incubation period shortening rate (%) Sleep duration (minutes) Sleep duration extension rate (%) Model control group 32.5 ± 5.2 - 48.6 ± 8.1 - Positive control group 18.1 ± 3.1** 44.3 98.3 ± 12.5** 102.3 Example 1 15.6 ± 2.5** 52.0 125.4 ± 15.6** 158.0 Example 2 17.8 ± 3.2** 45.2 102.5 ± 11.3** 110.9 Example 3 16.9 ± 2.9** 48.0 112.8 ± 13.7** 132.1 Comparative Example 1 28.3 ± 4.8 12.9 62.3 ± 9.2 28.2 Comparative Example 2 26.5 ± 4.1* 18.5 70.1 ± 10.4* 44.2 Comparative Example 3 24.2 ± 3.9* 25.5 78.5 ± 11.2* 61.5 Note: Compared with the model control group, *p < 0.05, **p < 0.01. Shortening rate / prolongation rate = (Model group value - Drug group value) / Model group value × 100%. Results analysis: The jujube extracts in Examples 1-3, at a dose of 200 mg / kg, significantly (p < 0.01) shortened the sleep latency of mice (by more than 45%) and prolonged the sleep time (by more than 110%), which was better than the positive control drug diazepam (especially in terms of prolonging sleep time).
[0047] Example 1 showed the best results, with a latency period reduction rate of 52.0% and a sleep duration extension rate of 158.0%, fully meeting the technical requirements of "≥40% and ≥80%" specified in this invention.
[0048] Comparative Example 1 (traditional water extract) and Comparative Example 2 (polysaccharide component only) showed poor results, with sleep duration extension rates both below 50%, indicating that the synergistic effect of the specific proportion of active ingredient groups in this invention is key.
[0049] Comparative Example 3 (using non-target molecular weight polysaccharides) showed significantly lower efficacy than Example 1, indicating that polysaccharides within a specific molecular weight range (10-50 kDa) are crucial to overall efficacy.
[0050] This invention provides a jujube extract with clearly defined components, advanced preparation process, significant and stable sleep-improving effects, and good prospects for industrial application.
[0051] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
[0052] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.
[0053] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.
Claims
1. A jujube extract with sleep-improving properties, characterized in that, The extract contains jujube polysaccharides, total jujube flavonoids, and total jujube triterpenoids in a weight ratio of 1:(0.3~0.6):(0.1~0.2); The molecular weight distribution of the jujube polysaccharide is as follows: the proportion of components with Mw10~50kDa is ≥65%, and the molar ratio of glucose, galactose and arabinose in the polysaccharide component is (2~4):(1~2):(1~1.5). The total flavonoids of the jujube contain at least 0.5 wt% of spinosin and 0.3 wt% of 6'''-feruloyl spinosin; In animal experiments, the jujube extract, administered by gavage at a dose of 100-300 mg / kg, shortened the sleep latency of sodium pentobarbital-induced mice by ≥40% and prolonged the sleep time by ≥80%.
2. The jujube extract with sleep-improving function according to claim 1, characterized in that, The extract also contains γ-aminobutyric acid (GABA) and 5-hydroxytryptamine, and the weight ratio of GABA to 5-hydroxytryptamine is 1:(0.02~0.05).
3. A method for preparing a jujube extract with sleep-improving function according to claims 1-2, characterized in that, Includes the following steps: (1) Pretreatment with compound enzyme hydrolysis: Remove the pits from the red dates, crush them to 20-60 mesh, add 3-8 times their weight of water, adjust the pH to 4.0-5.5, add compound enzyme, and hydrolyze at 40-55℃ for 1-3 hours to obtain the enzymatic hydrolysate; the compound enzyme is composed of cellulase, pectinase and xylanase in a weight ratio of (2-3):(1-2):(0.5-1), and the total amount of enzyme added is 0.5%-2% of the dry weight of the red dates; (2) Dynamic countercurrent extraction: The enzymatic hydrolysate is heated to 80~95℃ and continuously extracted in a dynamic countercurrent extraction device. The liquid-solid ratio of the extract to the material is (10~20):
1. The extraction time is 30~60 minutes. The extract is collected and the extraction residue is separated at the same time. (3) Gradient alcohol precipitation: Concentrate the extract to 1 / 5 to 1 / 3 of its original volume, add ethanol to 40% to 50% ethanol volume fraction, let it stand to precipitate, and collect the first precipitate (polysaccharide component A) by centrifugation; add ethanol to the supernatant to 75% to 85% ethanol volume fraction, let it stand to precipitate, and collect the second precipitate (polysaccharide component B) by centrifugation; combine polysaccharide components A and B to obtain the jujube polysaccharide; (4) Simulated moving bed chromatography purification: The final supernatant after two alcohol precipitations in step (3) was concentrated under reduced pressure to recover ethanol, and then separated by a simulated moving bed chromatography system. The stationary phase was C18 or phenyl bonded silica gel, and the mobile phase was an ethanol-water-acetic acid system. The total flavonoid fraction and the total triterpenic acid fraction were collected according to the retention time. (5) Targeted compounding: The jujube polysaccharide obtained in step (3), the total jujube flavonoids obtained in step (4) and the total triterpenic acid of jujube are compounded and mixed evenly according to the weight ratio described in claim 1, and then dried to obtain the product.
4. The method for preparing a jujube extract with sleep-improving function according to claim 1, characterized in that, Before the compound enzymatic hydrolysis in step (1), a low-frequency ultrasonic-assisted treatment step is also included: ultrasonic treatment for 10 to 20 minutes at 20 to 40 kHz and power density of 50 to 200 W / L to destroy the cell wall structure of jujube.
5. The method for preparing a jujube extract with sleep-improving function according to claim 1, characterized in that, The dynamic countercurrent extraction device in step (2) is a multi-stage spiral countercurrent extractor. During the extraction process, the linear velocity of the extract is maintained at 0.5~2cm / s, and nitrogen is continuously introduced during the extraction process to isolate oxygen and avoid oxidation of active ingredients.
6. The method for preparing a jujube extract with sleep-improving function according to claim 1, characterized in that, The operating parameters of the simulated moving bed chromatography system in step (4) are as follows: injection flow rate 1~5 mL / min, elution flow rate 20~50 mL / min, switching time 5~15 minutes, column temperature 25~40℃, and ethanol-water-acetic acid with a volume ratio of (15~30):(70~85):(0.5~2) as the mobile phase. The first fraction (total flavonoids) with a retention time of 0.8~1.5 times the switching time and the second fraction (total triterpenic acids) with a retention time of 2.0~3.5 times the switching time are collected respectively.
7. The method for preparing a jujube extract with sleep-improving function according to claim 1, characterized in that, The drying in step (5) is vacuum belt drying or low-temperature spray drying, with a drying temperature ≤60℃, to obtain an extract powder with good flowability and solubility.
8. The use of the jujube extract according to claim 1 or 2 in the preparation of drugs, health foods or functional foods with sleep-improving functions.