Auricularia auricula-judae fruiting body whole-cell polysaccharide for regulating abnormal glucose metabolism and application thereof

The extraction of whole-cell polysaccharides from black fungus fruiting bodies using a sequential coupling process of "enzyme first, oxygen later" solves the problem of low extraction efficiency in traditional methods, achieving efficient and environmentally friendly polysaccharide preparation. This process significantly improves abnormal sugar metabolism and gut microbiota dysbiosis, providing an effective means for regulating abnormal sugar metabolism.

CN122302118APending Publication Date: 2026-06-30CHINA JILIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA JILIANG UNIV
Filing Date
2026-04-16
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing technologies are insufficient for effectively extracting whole-cell polysaccharides from black fungus fruiting bodies, and their application value in regulating abnormal sugar metabolism has not been fully realized. This is mainly due to the unknown cell disruption methods and structural complexity, which lead to low extraction efficiency and easy loss of active structural domains.

Method used

The process employs a sequential coupling technique of "enzyme first, oxygen later". First, cellulase is used for gentle enzymatic hydrolysis, and then hydrogen peroxide is used to extract polysaccharides from the whole cells of black fungus fruiting bodies. Combined with ultrafine grinding and removal of small molecule impurities, the integrity of polysaccharide active fragments and efficient extraction are ensured.

Benefits of technology

The polysaccharide dissolution rate was significantly improved. The obtained whole-cell polysaccharides from black fungus fruiting bodies showed dual regulatory effects on glucose and lipid metabolism in vivo and in vitro. They could slow down weight gain caused by high-calorie diets and improve glucose and lipid metabolism disorders in diabetic mice, regulate intestinal flora structure, and produce specific metabolites.

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Abstract

This invention discloses a whole-cell polysaccharide from the fruiting body of *Auricularia auricula-judae* (black fungus) for regulating abnormal glucose metabolism and its applications. Using *Auricularia auricula-judae* as raw material, the polysaccharide undergoes pretreatment including ultrafine grinding and removal of small molecule impurities. First, it is gently enzymatically hydrolyzed using cellulase, followed by further extraction via hydrogen peroxide. This yields a high-yield, highly bioactive whole-cell polysaccharide from the fruiting body of *Auricularia auricula-judae*, offering advantages such as low cost, high efficiency, cleanliness, environmental friendliness, and preservation of the integrity of the polysaccharide's "active fragments" during cell wall disruption. The whole-cell polysaccharide extracted using this method significantly alleviates excessive weight gain in C57BL / 6J diabetic mice induced by a high-calorie diet combined with low-dose streptozotocin (STZ) and improves their glucose metabolism disorders. Simultaneously, this polysaccharide regulates the intestinal flora structure of mice, inducing the production of novel biomarkers such as tridecane, and also exhibits a significant regulatory effect on short-chain fatty acid content.
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