Method for preparing liupao tea polysaccharide by composite enzymolysis in cooperation

CN122302121APending Publication Date: 2026-06-30NANNING UNIV

Patent Information

Authority / Receiving Office: CN · China
Patent Type: Applications(China)
Current Assignee / Owner: NANNING UNIV
Filing Date: 2026-05-14
Publication Date: 2026-06-30

AI Technical Summary

Technical Problem

In existing methods for extracting polysaccharides from Liubao tea, harsh treatment conditions such as high temperature and strong shearing can easily damage the polysaccharide structure. Single or double enzymatic hydrolysis is inefficient, the extraction process is lengthy and polysaccharide loss is significant, making it difficult to achieve high purity and high yield of polysaccharides.

Method used

A synergistic process combining enzymatic hydrolysis and aqueous two-phase extraction was adopted. Cellulase, pectinase and papain worked together to degrade the cell wall of tea leaves, and aqueous two-phase extraction was used to achieve efficient dissolution and in-situ phase separation purification of polysaccharides, avoiding high temperature and multi-step purification.

Benefits of technology

It significantly improves the extraction rate and purity of polysaccharides, reduces protein residue, simplifies the process, enhances the antioxidant and hypoglycemic activities of polysaccharides, and is suitable for industrial production.

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Abstract

This invention discloses a method for synergistically preparing Liubao tea polysaccharides through compound enzymatic hydrolysis, belonging to the field of natural product extraction technology. The method comprises the following steps: pulverizing Liubao tea, defatting it, and then separating the solid and liquid phases to obtain defatted tea residue; mixing cellulase, pectinase, and papain to obtain a compound enzyme preparation; dispersing the defatted tea residue in water, then adding the compound enzyme preparation for enzymatic hydrolysis, followed by enzyme inactivation to obtain a mixed solution; separating the solid and liquid phases of the mixed solution to obtain a polysaccharide extract, adding it to an aqueous two-phase extraction solution, allowing it to stand for phase separation, separating the upper phase to obtain a polysaccharide-rich solution, concentrating it to obtain a polysaccharide concentrate, precipitating it with alcohol to obtain an alcohol precipitate, and then performing solid-liquid separation to collect the precipitate, thus obtaining the Liubao tea polysaccharides. This invention, by coupling the compound enzymatic hydrolysis process with the aqueous two-phase extraction process, can significantly improve both the polysaccharide extraction rate and purity of the product.

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