Steroid C1,2 dehydrogenase mutants and their application in the preparation of methylprednisolone dehydrogenases

By modifying steroid C1,2 dehydrogenase and introducing specific amino acid mutations, the enzyme activity and thermal stability were improved, solving the problem that enzyme activity is easily affected in the preparation of methylprednisolone dehydrogenase. This enabled an efficient and environmentally friendly enzymatic preparation method, reducing production costs and wastewater treatment volume.

CN122303165APending Publication Date: 2026-06-30HUANGGANG HUMANWELL PHARMACEUTICAL CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUANGGANG HUMANWELL PHARMACEUTICAL CO LTD
Filing Date
2026-05-11
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

In existing technologies, the preparation methods of methylprednisolone dehydrogenates suffer from low chemical dehydrogenation yield, high cost, and significant pollution, while the enzyme activity in biological dehydrogenation methods is easily affected by reaction conditions, making recovery and reuse difficult.

Method used

By modifying steroidal C1,2 dehydrogenase and introducing specific mutations into the amino acid sequence, the activity and thermostability of the enzyme are improved. Enzyme-solution reuse technology is used to reduce production costs and wastewater treatment volume.

Benefits of technology

It significantly improved enzyme activity and thermal stability, enabling efficient and environmentally friendly preparation of methylprednisolone dehydrogenates, and reducing production costs and wastewater treatment volume.

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Abstract

This invention relates to the field of enzyme modification technology and also to the field of pharmaceutical intermediate preparation, disclosing a steroidal C1,2 dehydrogenase mutant and its application in the preparation of methylprednisolone dehydrogenates. The amino acid sequence of the steroidal C1,2 dehydrogenase mutant is shown in SEQ ID NO:3. This invention modifies the steroidal C1,2 dehydrogenase, providing a C1,2 dehydrogenase mutant that significantly improves the activity and thermal stability of the steroidal C1,2 dehydrogenase; it exhibits good enzymatic preparation results for methylprednisolone dehydrogenates, and allows for enzyme solution reuse, reducing production costs and post-reaction wastewater treatment, making it environmentally friendly.
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