A β-2,6-fructan sucrase mutant and its application
By performing site-directed mutagenesis on β-2,6-fructan sucrase and optimizing its amino acid sequence, the problem of uneven product distribution was solved, enabling the targeted and efficient synthesis of high molecular weight β-2,6-fructan, reducing production costs, and promoting industrial application.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- JIANGNAN UNIV
- Filing Date
- 2026-04-17
- Publication Date
- 2026-06-30
AI Technical Summary
In existing β-2,6-fructan sucrase-catalyzed synthesis methods, the product distribution is uneven and the proportion of low molecular weight byproducts is high, resulting in high production costs and difficult processes, which limits the large-scale application of high molecular weight β-2,6-fructan.
By performing site-directed mutagenesis on β-2,6-fructan sucrase derived from Lactobacillus reuteri, specifically by mutating asparagine at position 223 to alanine or tryptophan, β-2,6-fructan sucrase mutants LrLS-N223A and LrLS-N223W were constructed, and their amino acid sequences were optimized to control the molecular weight distribution of the product.
It significantly increased the proportion of high molecular weight β-2,6-fructans, reduced the proportion of low molecular weight byproducts, simplified the downstream purification process, reduced production costs, and promoted the industrial application of β-2,6-fructan enzymatic synthesis technology.
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Figure CN122303181A_ABST