A method for detecting early pregnancy in bovine women based on bovine pregnancy-associated glycoproteins.

The detection method combining mRNA probe mixture and magnetic microparticle chemiluminescence analyzer solves the problems of complexity and professional dependence of existing dairy cow pregnancy diagnosis methods, and realizes early, accurate, non-invasive and automated dairy cow pregnancy detection, thereby improving reproductive efficiency and reducing costs.

CN122303223APending Publication Date: 2026-06-30HEXU (ZHENGZHOU) BIOTECHNOLOGY CO LTD
0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HEXU (ZHENGZHOU) BIOTECHNOLOGY CO LTD
Filing Date
2026-04-09
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing methods for diagnosing pregnancy in dairy cows suffer from problems such as long testing cycles, complex operations, high dependence on professional personnel, high costs, or limited applicability, failing to meet the needs of the livestock industry for early, rapid, accurate, and automated pregnancy diagnosis.

Method used

The mRNA probe mixture was used for detection, and the paired detection of bovine pregnancy-related glycoprotein mRNA was achieved by using a magnetic microparticle chemiluminescence analyzer. The sampling rod was used to pick up the sample and combined with magnetic bead adsorption and chemiluminescence technology to achieve fully automated detection.

Benefits of technology

It enables early, accurate, non-invasive, and convenient detection of pregnancy in dairy cows. The detection speed is fast, it is suitable for large-scale batch testing, reduces reliance on personnel and operational errors, and the test results are highly stable, significantly improving reproductive efficiency and reducing breeding costs.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure SMS_1
    Figure SMS_1
  • Figure SMS_2
    Figure SMS_2
  • Figure SMS_3
    Figure SMS_3
Patent Text Reader

Abstract

This invention belongs to the field of veterinary testing technology, specifically relating to a method for detecting early pregnancy in cows based on bovine pregnancy-related glycoproteins. The method includes: sampling the uterine wall of a cow 21 days after insemination using a sampling stick dipped in a special sampling solution; immersing the sampling stick in lysis buffer and then removing it; adding mRNA adsorption magnetic beads to the sample solution, shaking to adsorb the adsorption, and washing; subsequently adding an mRNA probe mixture, shaking to react, and then detecting the sample. The mixed probe primer and detection method of this invention enable early, accurate, convenient, low-cost, and automated detection of early pregnancy in dairy cows. It effectively solves the shortcomings of existing technologies, such as slow response time, cumbersome operation, high cost, and reliance on professional personnel. This is of great significance for improving reproductive efficiency and reducing breeding costs, and has broad application prospects.
Need to check novelty before this filing date? Find Prior Art