Nucleic acid detection methods for internal control substrate nucleic acid molecules, nucleic acid compositions, and substrate correction.

Abstract

This application relates to the field of biological detection technology, and particularly to an internal control base nucleic acid molecule, a nucleic acid composition, and a base-corrected nucleic acid detection method. It further relates to a reagent kit, and the application of the internal control base nucleic acid molecule, nucleic acid composition, and reagent kit in the preparation of products for detecting pathogens. The internal control base nucleic acid molecule is amplified in a nucleic acid amplification reaction and contains a first nucleic acid fragment and a second nucleic acid fragment. The first nucleic acid fragment is located within the target region amplified by the detection primer pair on the internal control base nucleic acid molecule, and the detection probe specifically hybridizes with the first nucleic acid fragment in the nucleic acid amplification reaction. The second nucleic acid fragment does not specifically hybridize with the detection probe under the same reaction conditions as the nucleic acid amplification reaction. The base-corrected nucleic acid amplification reaction incorporating this internal control base nucleic acid molecule significantly improves the stability and sensitivity of the detection.

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