Sample pretreatment method and application thereof

By using protease pretreatment reagents to disrupt antibody structures, the problems of cumbersome sample dilution and errors are solved, thus improving the accuracy and efficiency of immunoassay.

CN122306525APending Publication Date: 2026-06-30CHEMCLIN DIAGNOSTICS CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHEMCLIN DIAGNOSTICS CO LTD
Filing Date
2024-12-31
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

In immunoassay, sample dilution is a cumbersome process that can lead to errors in test results. Furthermore, the dilution step requires changing pipette tips multiple times, which affects the efficiency and accuracy of the test.

Method used

Undiluted test samples are treated with pretreatment reagents containing proteases. By destroying the antibody structure, denaturing or degrading the antibody, interfering antibodies are removed, simplifying the sample pretreatment process.

Benefits of technology

It improved the accuracy and sensitivity of test results, reduced the false positive rate, simplified the operation process, and shortened the testing time.

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Abstract

This application relates to a sample pretreatment method and its application. The method includes adding a pretreatment reagent to an undiluted test sample for sample pretreatment, resulting in a pretreated test sample that can be directly used for immunoassay. The pretreatment reagent destroys interfering antibodies in the test sample by disrupting antibody structure, and / or denaturing or degrading antibodies. The solution provided in this application can replace sample dilution, reduce or eliminate interference from interfering substances present in the sample, reduce false positive rates and the hook effect, improve the accuracy of immunoassay, and shorten the detection time.
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