Kit for detecting glial fibrillary acidic protein by photochemical chemiluminescence method and use method thereof

By using an oxygen-generating agent and catalyst in photo-induced chemiluminescence to generate oxygen, which is then activated into reactive oxygen species, and combines with luminescent microparticles and biomolecules to form a complex, the instability problem of detecting low-concentration colloidal fibrous acidic protein is solved, achieving higher detection stability and sensitivity.

CN122306784APending Publication Date: 2026-06-30CHEMCLIN DIAGNOSTICS CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHEMCLIN DIAGNOSTICS CO LTD
Filing Date
2024-12-31
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing photo-induced chemiluminescence methods suffer from low signal values ​​and are susceptible to noise interference when detecting low concentrations of glial fibrillary acidic proteins, resulting in unstable test results and poor precision, making it difficult to effectively identify and assess neurological diseases.

Method used

A kit containing an oxygen-generating agent, a catalyst, luminescent microparticles, and a labeled biomolecule is used. The oxygen-generating agent generates oxygen under the action of a catalyst, which is then activated into reactive oxygen species to enhance the light signal. The luminescent microparticles specifically bind to the biomolecules to form a complex that generates a detectable light signal.

Benefits of technology

It improves the detection stability, sensitivity, and precision of low-concentration glial fiber acidic protein, enhances the light signal intensity, and improves the accuracy and reliability of detection.

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Abstract

This application relates to a kit and method for detecting glial fibrillary acidic protein using photo-induced chemiluminescence (PET). The kit includes: reagent 1 comprising an oxygen-generating agent; reagent 2 comprising a catalyst; reagent 3 comprising luminescent microparticles and a first biomolecule bound thereto; and reagent 4 comprising a labeled second biomolecule. The oxygen-generating agent reacts with the catalyst to generate oxygen, which increases the oxygen content in the reaction system during PET detection. The luminescent microparticles react with reactive oxygen species to generate a detectable light signal. Both the first and second biomolecules specifically bind to glial fibrillary acidic protein in the sample. The method provided in this application can increase the light signal value even at low concentrations of glial fibrillary acidic protein, thus improving the detection capability.
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