An electrochemical detection method based on nucleic acid modified interfaces

By constructing an electrochemical sensor through nucleic acid modification of the interface, the problems of disorder and non-specific interference at the electrochemical sensing interface are solved, enabling highly sensitive quantitative analysis of the novel tumor marker HERV-H Env protein. It is applicable to various electrode substrates and signal conversion logics, and can be adapted to portable detection terminals to meet the needs of rapid clinical detection.

CN122306903APending Publication Date: 2026-06-30SHANGHAI JIAOTONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAOTONG UNIV
Filing Date
2026-03-23
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing biosensing technologies suffer from disorder and non-specific interference when constructing electrochemical sensing interfaces, making it difficult to achieve highly sensitive quantitative analysis of novel tumor markers such as HERV-H Env protein. Furthermore, they lack a platform-based universal detection architecture, failing to meet the needs of rapid clinical testing.

Method used

An electrochemical detection method based on nucleic acid modified interfaces is adopted. A regular biofunctionalized layer is formed by the self-assembly of nucleic acid chains. Combined with a signal-off type electrochemical response, the precise positioning and spacing of recognition molecules on the electrode surface are achieved. This method is adaptable to various electrode substrates and signal conversion logics, and a universal analysis architecture is constructed.

Benefits of technology

It significantly improves the signal-to-noise ratio and reproducibility of detection, enables highly sensitive quantitative analysis in complex biological samples, is suitable for portable detection terminals, is compatible with various electrode systems and signal conversion logics, and is applicable to the detection of standard solutions to real plasma samples.

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Abstract

This invention discloses an electrochemical detection method based on a nucleic acid-modified interface. It first relates to a universally applicable electrochemical detection platform for HERV-H Env protein based on a nucleic acid-modified interface, with colorectal cancer detection as a typical application example. The detection method includes the following core steps: preparation of nucleic acid chains and their self-assembled functional nucleic acid structures; construction of a nucleic acid-modified electrode interface; coupling of a specific recognition molecule antibody with the modified nucleic acid; target molecule capture; and electrochemical signal readout. According to this invention, a scheme is provided for target protein detection based on a nucleic acid-modified interface and a targeted immobilization technique for recognition molecules, using an electrochemical signal amplification system to achieve target detection. This scheme can be applied not only to colorectal cancer diagnosis and screening but also extended to research on other tumors, chronic diseases, and aging. This invention possesses high specificity, high sensitivity, and high reproducibility, and has good potential for clinical application and translation.
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