A collagen tripeptide composition, and a preparation method and application thereof

Abstract

The application discloses a collagen tripeptide composition and a preparation method and application thereof. The collagen tripeptide composition comprises the following raw materials in parts by weight: skate collagen peptide 80-200 parts, collagen tripeptide 70-120 parts, French fruit powder 100-300 parts, roxburgh rose fruit powder 300-600 parts, salvia extract 300-500 parts, tremella polysaccharide 100-150 parts, medlar powder 300-500 parts and radix astragali powder 100-300 parts. The collagen tripeptide composition can reduce the activity of beta-galactosidase, has a good anti-aging effect, and can quickly supplement collagen protein and maintain skin elasticity by applying the collagen tripeptide composition.

Description

Technical Field

[0001] This invention relates to a collagen tripeptide composition, its preparation method, and its application. Background Technology

[0002] Collagen is produced by fibroblasts, and their ability to synthesize collagen declines with age. When the body experiences oxidative stress, excess reactive oxygen species (ROS) accumulate, leading to oxidative damage. This slows the growth and renewal of fibroblasts, reducing their ability to synthesize collagen and elastin fibers, resulting in collagen loss, decreased moisture content, and skin aging. In an aging state, the activity of antioxidant enzymes decreases, leading to a decline in total antioxidant capacity and glutathione levels, disrupting the body's balance and causing protein oxidation, further accelerating aging. Skin aging results in dullness and dryness, thus requiring continuous collagen replenishment to maintain youthful skin.

[0003] Collagen peptides (CP) are mixtures between amino acids and proteins formed by enzymatic hydrolysis of collagen. They are small molecule peptides with molecular weights ranging from several hundred to several thousand Daltons. Compared to collagen, CP has a relatively low molecular weight and is easily absorbed by the intestinal wall due to its water solubility. It also possesses good bioavailability, antioxidant properties, and low allergenicity.

[0004] Patent document CN109984281A discloses a protein peptide beverage, the raw materials of which, by weight, include: 7-9% fructooligosaccharides, 5-7% grapefruit concentrate, 4-6% refined fine sugar, 2-4% carrot concentrate, 2-4% collagen peptides, 1-3% konjac powder, 1-3% chlorella, 1-3% coix seed, 0.5-1% moringa enzyme powder, 0.5-1% compound fruit and vegetable enzyme powder, 0.5-1% inulin, 0.06% sodium citrate, 0.04% potassium sorbate, 0.2% grape flavoring, and 65-70% water. The protein peptide beverage provided by this invention can replenish a large amount of collagen that is easily absorbed by the human body. Journal article: Collagen peptide solid beverage with significant cosmetic effects and broad prospects (Zhang Lu et al., China Food, December 2020). This study investigated the skin-improving effects of a solid beverage composed of fish collagen peptide powder as the main raw material, combined with pomegranate juice powder and yeast extract. The results showed that the collagen peptide solid beverage can significantly increase skin elasticity.

[0005] Collagen peptides can be rapidly absorbed by the body, stimulating the production of endogenous collagen, replenishing collagen synthesis, and enhancing skin elasticity. Maximizing their anti-aging effects is currently a key focus of research. Summary of the Invention

[0006] The purpose of this invention is to provide a collagen tripeptide composition, its preparation method, and its applications. This collagen tripeptide composition can effectively reduce the activity of β-galactosidase and has a good anti-aging effect.

[0007] Terminology section: All numerical values ​​or expressions relating to component quantities, process conditions, etc., used herein shall be understood to be modified by “about” in all cases. When referring to a quantity or range of values, the quantity or range is an approximation within experimental variability (or within statistical experimental error). In this document, the term “about” shall have the meaning of being within 10%, preferably within 5%, of the specified value or range.

[0008] As used herein, the term "room temperature" refers to ambient temperature, ranging from about 10°C to about 40°C. In some embodiments, "room temperature" refers to a temperature ranging from about 20°C to about 30°C; in other embodiments, "room temperature" refers to a temperature ranging from about 25°C to about 30°C; and in still other embodiments, "room temperature" refers to 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, etc.

[0009] The term "pulverization" as used in this article refers to the process of breaking down solid lumps or granules into fine particles, fine powders, or ultrafine powders through physical or mechanical methods such as impact, shearing, grinding, and shock. This process can disrupt the material's structure, increase its specific surface area, and improve its dissolution and extraction efficiency. Among these, mechanical pulverization, air jet milling, ultrafine pulverization, cryogenic pulverization, or grinding pulverization are all conventional pulverization methods in this field.

[0010] As used in this article, "solid-liquid separation" refers to the process of separating a mixture containing solid particles and liquid components into independent solid and liquid phases using physical or mechanical methods. Centrifugation, filtration, sedimentation, and pressure filtration are all conventional solid-liquid separation methods in this field.

[0011] As used in this article, "drying" refers to the removal of free moisture, solvents, and volatile components from the interior and surface of materials, raw materials, pharmaceutical intermediates, or equipment through physical or thermal methods such as volatilization and evaporation, thereby reducing the moisture content of the material. Among these methods, atmospheric pressure drying, vacuum drying, freeze drying, and spray drying are all conventional drying techniques in this field.

[0012] To achieve the above objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides a collagen tripeptide composition for skin anti-aging, comprising the following raw materials: ray collagen peptide, collagen tripeptide, French flower and fruit powder, prickly pear powder, sage extract, tremella polysaccharide, wolfberry powder and astragalus powder.

[0013] In some embodiments, the collagen tripeptide composition comprises the following raw materials in parts by weight: 80-200 parts of ray collagen peptide, 70-120 parts of collagen tripeptide, 100-300 parts of French flower and fruit powder, 300-600 parts of prickly pear powder, 300-500 parts of sage extract, 100-150 parts of tremella polysaccharide, 300-500 parts of wolfberry powder, and 100-300 parts of astragalus powder.

[0014] In some embodiments, the total weight of the ray collagen peptides and collagen tripeptides is 15%-25% of the total weight of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide; preferably 18%-21%.

[0015] In some embodiments, the weight ratio of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide is 10-30:30-60:35-45:10-14; preferably 15:50:40:12.

[0016] In some preferred embodiments, the weight ratio of the ray collagen peptides to collagen tripeptides is 8-20:7-12; preferably 2:1.

[0017] In some embodiments, the collagen tripeptide composition comprises the following raw materials in parts by weight: 160 parts of ray collagen peptide, 80 parts of collagen tripeptide, 150 parts of French flower and fruit powder, 500 parts of prickly pear powder, 400 parts of sage extract, 120 parts of tremella polysaccharide, 400 parts of wolfberry powder, and 200 parts of astragalus powder.

[0018] In some embodiments, the collagen tripeptide composition further includes grapefruit juice powder and orange powder.

[0019] Preferably, the amount of grapefruit juice powder in the collagen tripeptide composition is 500-1000 parts; the amount of orange powder is 800-1600 parts; more preferably, the amount of grapefruit juice powder is 550-950 parts; and the amount of orange powder is 1000-1400 parts.

[0020] In some embodiments, the collagen tripeptide composition comprises the following raw materials in parts by weight: 160 parts of ray collagen peptide, 80 parts of collagen tripeptide, 150 parts of French flower and fruit powder, 500 parts of prickly pear powder, 400 parts of sage extract, 120 parts of tremella polysaccharide, 400 parts of wolfberry powder, 200 parts of astragalus powder, 500-1000 parts of grapefruit juice powder, and 800-1600 parts of orange powder.

[0021] In some embodiments, the collagen tripeptide composition comprises the following raw materials in parts by weight: 160 parts of ray collagen peptide, 80 parts of collagen tripeptide, 150 parts of French flower and fruit powder, 500 parts of prickly pear powder, 400 parts of sage extract, 120 parts of tremella polysaccharide, 400 parts of wolfberry powder, 200 parts of astragalus powder, 750 parts of grapefruit juice powder, and 1200 parts of orange powder.

[0022] In some embodiments, the preparation method of the collagen tripeptide composition includes the following steps: (1) Add water to the astragalus powder and wolfberry powder and decoct them. Separate the solid and liquid to obtain a clear liquid; (2) Add ray collagen peptides and collagen tripeptides to the clear liquid, dissolve and dry to obtain mixed peptide powder; (3) Mix the mixed peptide powder with the remaining raw materials.

[0023] Preferably, the amount of water used in step (1) is 12-18 times the total weight of Astragalus membranaceus and Lycium barbarum; the decoction time is 1-3 hours.

[0024] More preferably, the amount of water used in step (1) is 15 times the total weight of Astragalus membranaceus and Lycium barbarum; the reflux extraction time is 2 hours.

[0025] Preferably, the cooling in step (1) is cooling to below 40°C.

[0026] Preferably, the drying in step (1) is to reduce the moisture content of the herbal compound powder to less than 5%; more preferably, it is spray drying.

[0027] The beneficial effects of this invention are as follows: (1) This invention utilizes French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide to combine with ray collagen peptides and collagen tripeptides, which can promote the absorption of ray collagen peptides and collagen tripeptides, thereby achieving the purpose of quickly replenishing collagen. At the same time, the interaction between the components can effectively reduce the activity of β-galactosidase and enhance the anti-aging effect of the skin.

[0028] (2) In this invention, the clear liquid obtained by boiling Astragalus powder and wolfberry powder with water is sprayed with ray collagen peptides and collagen tripeptides to reduce the damage of gastric acid to collagen peptides and improve intestinal absorption. At the same time, the active ingredients of Astragalus and wolfberry can also combine with collagen peptides to synergistically promote skin elasticity and reduce wrinkles. Attached Figure Description

[0029] Figure 1 The image shows the staining intensity of zebrafish β-galactosidase after sample treatment, with the area within the yellow dashed box representing the analysis region.

[0030] Figure 2The staining intensity of zebrafish β-galactosidase after sample treatment was ns, p > 0.05 compared with the model control group. p > 0.01; , p>0.001. Detailed Implementation

[0031] The following description of the embodiments is merely to aid in understanding the method and core ideas of the present invention. It should be noted that those skilled in the art can make various modifications and improvements to the present invention without departing from its principles, and these modifications and improvements also fall within the scope of the claims. The following description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the present invention is not limited to the embodiments shown herein, but can be applied to a wider scope consistent with the principles and novel features disclosed herein. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains.

[0032] The following examples and comparative examples of the present invention are shown to provide a more detailed description of the present invention. However, the present invention is not limited thereto. Unless otherwise specified, all percentages in the present invention are mass percentages.

[0033] The present invention does not limit the source of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are all commercially available products in this technical field.

[0034] The following sources of raw materials are provided as examples: Ray collagen peptides, with collagen ≥62% and glycosaminoglycans (GAGs) ≥37%; Collagen tripeptide, oligopeptide is approximately 88.2%; French flower and fruit powder, hydroxytyrosol ≥1.5%, oligomeric proanthocyanidins approximately 36.5%; Prickly pear powder contains approximately 18.1% Vitamin C and approximately 133,000 u / g of Superoxide Dismutase (SOD). Sage extract, with approximately 5.6% rosmarinic acid; The polysaccharide content of Tremella fuciformis is approximately 96.5% mannan. Goji berry powder, containing 30% goji berry polysaccharides; Astragalus powder, containing 0.19% astragaloside A; Grapefruit juice powder, purchased from Topcon Health Technology (Nantong) Co., Ltd. Orange powder, purchased from Tianjin Zhenruguo Food Industry Co., Ltd. Black chokeberry powder, purchased from Ningxia Xiangcao Biotechnology Co., Ltd.

[0035] Example 1: Collagen Tripeptide Composition for Skin Anti-aging The collagen tripeptide composition is composed of the following raw materials in parts by weight: 160 parts of ray collagen peptide, 80 parts of collagen tripeptide, 150 parts of French flower and fruit powder, 500 parts of prickly pear powder, 400 parts of sage extract, 120 parts of tremella polysaccharide, 200 parts of wolfberry powder and 200 parts of astragalus powder. in, The weight ratio of the ray collagen peptides to collagen tripeptides is 2:1; The weight ratio of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide is 15:50:40:12. The total weight of the ray collagen peptides and collagen tripeptides is 21% of the total weight of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide.

[0036] The preparation method is as follows: (1) Add water at 15 times the total weight of Astragalus powder and Lycium barbarum powder to the powder and decoct for 2 hours. Centrifuge at 3000 rpm for 10 minutes to obtain a clear liquid. (2) Cool the clear liquid to room temperature, add ray collagen peptides and collagen tripeptides to dissolve, and spray dry (inlet air temperature 80℃, outlet air temperature 50℃) for 12 hours to obtain mixed peptide powder; (3) Mix the mixed peptide powder with the remaining raw materials, crush it, and pass it through a 100-mesh sieve.

[0037] Example 2: Collagen Tripeptide Composition for Skin Anti-aging The collagen tripeptide composition is composed of the following raw materials in parts by weight: 80 parts of ray collagen peptide, 70 parts of collagen tripeptide, 100 parts of French flower and fruit powder, 300 parts of prickly pear powder, 300 parts of sage extract, 100 parts of tremella polysaccharide, 300 parts of wolfberry powder and 100 parts of astragalus powder. in, The weight ratio of the ray collagen peptides to collagen tripeptides is 8:7; The weight ratio of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide is 1:3:3:1; The total weight of the ray collagen peptides and collagen tripeptides is 19% of the total weight of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide.

[0038] The preparation method is the same as in Example 1.

[0039] Example 3: Collagen tripeptide composition for skin anti-aging The collagen tripeptide composition is composed of the following raw materials in parts by weight: 200 parts of ray collagen peptide, 120 parts of collagen tripeptide, 300 parts of French flower and fruit powder, 600 parts of prickly pear powder, 500 parts of sage extract, 150 parts of tremella polysaccharide, 500 parts of wolfberry powder and 300 parts of astragalus powder. in, The weight ratio of the ray collagen peptides and collagen tripeptides is 5:3; The weight ratio of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide is 6:12:10:3. The total weight of the ray collagen peptides and collagen tripeptides is 21% of the total weight of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide.

[0040] The preparation method is the same as in Example 1.

[0041] Comparative Example 1: Collagen Tripeptide Composition for Skin Anti-aging The difference between this comparative example and Example 1 is that the total weight of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide is the same, but the weight ratio is different.

[0042] Specifically, the collagen tripeptide composition is composed of the following raw materials in parts by weight: 160 parts of ray collagen peptide, 80 parts of collagen tripeptide, 80 parts of French flower and fruit powder, 330 parts of prickly pear powder, 560 parts of sage extract, 200 parts of tremella polysaccharide, 200 parts of wolfberry powder and 200 parts of astragalus powder. in, The weight ratio of the ray collagen peptides to collagen tripeptides is 2:1; The weight ratio of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide is 8:33:56:20. The total weight of the ray collagen peptides and collagen tripeptides is 21% of the total weight of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide.

[0043] The preparation method is the same as in Example 1.

[0044] Comparative Example 2: Collagen Tripeptide Composition for Skin Anti-aging The difference between this comparative example and Example 1 is that the total weight of the ray collagen peptides and collagen tripeptides remains the same, but their weight ratio is different.

[0045] Specifically, the collagen tripeptide composition is composed of the following raw materials in parts by weight: 40 parts of ray collagen peptide, 200 parts of collagen tripeptide, 150 parts of French flower and fruit powder, 500 parts of prickly pear powder, 400 parts of sage extract, 120 parts of tremella polysaccharide, 200 parts of wolfberry powder and 200 parts of astragalus powder. in, The weight ratio of the ray collagen peptides and collagen tripeptides is 1:5; The weight ratio of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide is 15:50:40:12. The total weight of the ray collagen peptides and collagen tripeptides is 21% of the total weight of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide.

[0046] The preparation method is the same as in Example 1.

[0047] Comparative Example 3: Collagen Tripeptide Composition for Skin Anti-aging The difference between this comparative example and Example 1 is that black chokeberry powder is used instead of prickly pear powder.

[0048] Specifically, the collagen tripeptide composition is composed of the following raw materials in parts by weight: 160 parts of ray collagen peptide, 80 parts of collagen tripeptide, 150 parts of French flower and fruit powder, 500 parts of black chokeberry powder, 400 parts of sage extract, 120 parts of tremella polysaccharide, 200 parts of wolfberry powder and 200 parts of astragalus powder. in, The weight ratio of the ray collagen peptides to collagen tripeptides is 2:1; The weight ratio of the French flower and fruit powder, black chokeberry powder, sage extract and tremella polysaccharide is 15:50:40:12. The total weight of the ray collagen peptides and collagen tripeptides is 21% of the total weight of the French flower and fruit powder, black chokeberry powder, sage extract and tremella polysaccharide.

[0049] The preparation method is the same as in Example 1.

[0050] Comparative Example 4: Collagen Tripeptide Composition for Skin Anti-aging The difference between this comparative example and Example 1 is that the total weight of the wolfberry powder and the astragalus powder is the same, but the dosage is different.

[0051] Specifically, the collagen tripeptide composition is composed of the following raw materials in parts by weight: 160 parts of ray collagen peptide, 80 parts of collagen tripeptide, 150 parts of French flower and fruit powder, 500 parts of prickly pear powder, 400 parts of sage extract, 120 parts of tremella polysaccharide, 50 parts of wolfberry powder and 350 parts of astragalus powder. in, The weight ratio of the ray collagen peptides to collagen tripeptides is 2:1; The weight ratio of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide is 15:50:40:12. The total weight of the ray collagen peptides and collagen tripeptides is 21% of the total weight of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide.

[0052] The preparation method is the same as in Example 1.

[0053] Comparative Example 5: Collagen Tripeptide Composition for Skin Anti-aging The difference between this comparative example and Example 1 is that the preparation methods are different.

[0054] Specifically, the collagen tripeptide composition is composed of the following raw materials in parts by weight: 160 parts of ray collagen peptide, 80 parts of collagen tripeptide, 150 parts of French flower and fruit powder, 500 parts of prickly pear powder, 400 parts of sage extract, 120 parts of tremella polysaccharide, 200 parts of wolfberry powder and 200 parts of astragalus powder. The weight ratio of the ray collagen peptide to the collagen tripeptide is 2:1. The weight ratio of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide is 15:50:40:12. The total weight of the ray collagen peptides and collagen tripeptides is 21% of the total weight of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide.

[0055] The preparation method is as follows: (1) Add water at 15 times the total weight of Astragalus powder and Lycium barbarum powder to Astragalus powder and Lycium barbarum powder and decoct for 2 hours. Centrifuge at 3000 rpm for 10 minutes to obtain clear liquid. Dry the clear liquid by spraying (inlet air temperature 80℃, outlet air temperature 50℃) for 12 hours to obtain Chinese medicine powder. (2) Mix the Chinese medicine powder with the remaining raw materials, crush it, and pass it through a 100-mesh sieve.

[0056] 1. Anti-aging effects Reagents and consumables: Wild-type AB strain zebrafish embryos (purchased from Huante Biotechnology, broodstock were 5-6 months old), methylcellulose (Sigma, CAS: 497-76-7), hydrogen peroxide (analytical grade), catalase (S10037, Shanghai Yuanye Biotechnology Co., Ltd.), cell senescence β-galactosidase staining kit (Shanghai Beyotime Biotechnology Co., Ltd.), culture flasks, glass slides, aquariums and wide-mouth bottles.

[0057] Instruments: Biochemical incubator, electronic balance (1 / 1000), six-well culture plate, stereo microscope, microscope photography system.

[0058] 1.1 Determination of the maximum tolerated concentration of the collagen tripeptide composition Normally developed AB strain zebrafish embryos (1 dpf) were randomly selected and placed in six-well plates (30 embryos per well). The standard dilution water was removed from the six-well plates without harming the embryos. 3 mL of the corresponding concentration of the test sample dilution was quickly added to each well. Except for the blank control group, 0.5 mmol / L hydrogen peroxide was added to all other groups to establish a zebrafish aging model. The culture plate was covered and wrapped with aluminum foil, and incubated in a (28.5±1)℃ biochemical incubator in the dark for 6 days. Fresh culture medium was required daily for each group. After 6 days, the maximum tolerated concentration of the sample for zebrafish was determined. The results are shown in Table 1 below.

[0059] Table 1. Results of the determination of the maximum tolerated concentration in each group of zebrafish.

[0060] The results showed that the maximum tolerated concentration of the collagen tripeptide compositions provided in the embodiments and comparative examples of the present invention was 6.25 µg·mL. -1 .

[0061] 1.2 Evaluation of the inhibitory effect of collagen tripeptide composition on β-galactosidase activity 1.2.1 Experimental Grouping Group according to the following method: Blank control group: containing zebrafish embryos and 3 mL of standard dilution water, with the solution changed daily during the period; Model control group: 0.5 mmol / L hydrogen peroxide prepared with zebrafish embryos and standard dilution water, with the solution changed daily during the period; Test group: containing zebrafish embryos, 0.5 mmol / L hydrogen peroxide prepared with standard dilution water, and 3.15 µg / mL... -1 The collagen tripeptide composition was changed daily during the process. 1.2.2 Observation and photography After being treated with the test substance at 28°C for 6 days, zebrafish from each experimental group were stained using a cell senescence β-galactosidase staining kit. After staining, at least 10 zebrafish were randomly selected from each experimental group, fixed with 3wt% methylcellulose, and observed and photographed under a stereomicroscope.

[0062] 1.2.3 Data Processing The staining intensity (A) of β-galactosidase in zebrafish was quantitatively analyzed using Image Pro Plus software. The staining intensity of β-galactosidase is shown in the figure below. Figure 1The selected area in the example analysis region diagram is shown. The statistical analysis results of this index were used to evaluate the sample's ability to inhibit hydrogen peroxide-induced enhancement of β-galactosidase activity. Statistical results are expressed as mean ± SE. Graphpad Prism software was used for plotting and statistical analysis; p < 0.05 indicated a statistically significant difference.

[0063] Formula for calculating the inhibition rate of β-galactosidase activity: C=(A1-A2) / (A1-A0)×100%, In the formula, C is the inhibition rate of β-galactosidase activity, %; A0 is the average staining intensity of β-galactosidase in the blank control group (n=10); A1 is the average staining intensity of β-galactosidase in the model control group (n=10); and A2 is the average staining intensity of β-galactosidase in the test group (n=10).

[0064] 1.2.4 Experimental Results Each group was treated with zebrafish at the maximum tolerated concentration obtained from the above tests, and the results are as follows: Figure 1 , Figure 2 As shown in Table 2.

[0065] Table 2. Results of β-galactosidase activity assay in zebrafish of each group.

[0066] Note: Compared with the model control group, ns, p > 0.05; p > 0.01; , p>0.001.

[0067] The results showed that the staining intensity of zebrafish in the model control group was higher than that in the blank control group, and the difference was extremely significant (p<0.001), indicating that the activity of β-galactosidase in zebrafish was significantly increased, and the zebrafish aging model was successfully established.

[0068] Compared with the model control group, the β-galactosidase activity in zebrafish in the Example 1-Example 3 groups was reduced, and the difference was statistically significant (p<0.01) or significant (p<0.001), indicating that the collagen tripeptide compositions prepared in Example 1-Example 3 have anti-aging effects.

[0069] Compared with the model control group, the β-galactosidase activity in zebrafish in Comparative Examples 1-5 was slightly reduced, but the difference was not statistically significant (p>0.05), indicating that the collagen tripeptide compositions prepared in Comparative Examples 1-5 do not have anti-aging effects.

[0070] As can be seen from Example 1 and Comparative Examples 1, 2, and 3, changes in the efficacy ingredients or their dosage relationships have a significant impact on the realization of the technical effects of the present invention. In the present invention, only when French flower and fruit powder, prickly pear powder, sage extract, and tremella polysaccharide are combined with ray collagen peptides and collagen tripeptides in a specific dosage relationship can the technical effects of the present invention be achieved.

[0071] As can be seen from Example 1 and Comparative Examples 4 and 5, the dosage relationship of wolfberry powder and astragalus powder, as well as their treatment methods for ray collagen peptides and collagen tripeptides, have a significant impact on the realization of the technical effect of the present invention. The technical effect of the present invention can only be achieved by combining the specific dosage relationship of wolfberry powder and astragalus powder with their spray granulation process for ray collagen peptides and collagen tripeptides.

[0072] The above description, in conjunction with specific embodiments, further illustrates the present invention. However, these embodiments are merely exemplary and do not constitute any limitation on the scope of the present invention. Those skilled in the art should understand that modifications or substitutions to the details and form of the technical solutions of the present invention can be made without departing from the spirit and scope of the invention, and all such modifications and substitutions fall within the protection scope of the present invention.

Claims

1. A collagen tripeptide composition, characterized in that, The ingredients include the following parts by weight: 80-200 parts of ray collagen peptide, 70-120 parts of collagen tripeptide, 100-300 parts of French flower and fruit powder, 300-600 parts of prickly pear powder, 300-500 parts of sage extract, 100-150 parts of tremella polysaccharide, 300-500 parts of wolfberry powder, and 100-300 parts of astragalus powder; French flower and fruit powder, with hydroxytyrosol ≥1.5% and oligomeric proanthocyanidins 36.5%.

2. The collagen tripeptide composition according to claim 1, characterized in that, The total weight of the ray collagen peptides and collagen tripeptides is 15%-25% of the total weight of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide.

3. The collagen tripeptide composition according to claim 1, characterized in that, The weight ratio of the French flower and fruit powder, prickly pear powder, sage extract and tremella polysaccharide is 10-30:30-60:35-45:10-14.

4. The collagen tripeptide composition according to claim 1, characterized in that, It also includes 500-1000 portions of grapefruit juice powder and 800-1600 portions of orange powder.

5. The collagen tripeptide composition according to claim 1, characterized in that, The ingredients include the following parts by weight: 160 parts ray collagen peptide, 80 parts collagen tripeptide, 150 parts French flower and fruit powder, 500 parts prickly pear powder, 400 parts sage extract, 120 parts tremella polysaccharide, 400 parts goji berry powder, 200 parts astragalus powder, 500-1000 parts grapefruit juice powder, and 800-1600 parts orange powder.

6. The collagen tripeptide composition according to claim 5, characterized in that, It is composed of the following ingredients in parts by weight: 160 parts ray collagen peptide, 80 parts collagen tripeptide, 150 parts French flower and fruit powder, 500 parts prickly pear powder, 400 parts sage extract, 120 parts tremella polysaccharide, 400 parts goji berry powder, 200 parts astragalus powder, 750 parts grapefruit juice powder, and 1200 parts orange powder.

7. A method for preparing the collagen tripeptide composition according to any one of claims 1-6, characterized in that, Includes the following steps: (1) Add water to Astragalus powder and Lycium barbarum powder and decoct for 1-3 hours, then separate the solid and liquid to obtain a clear liquid; (2) Add ray collagen peptides and collagen tripeptides to the clear liquid, dissolve and dry to obtain mixed peptide powder; (3) Mix the mixed peptide powder with the remaining raw materials.

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