Cosmetic lotion enriched with water-soluble beta-glucan and method for its preparation

Abstract

The application discloses a cosmetic water rich in water-soluble beta-glucan and a preparation method thereof, and belongs to the technical field of cosmetics. According to the cosmetic water, the mass fraction of the following components is comprised: beta-glucan 0.2-1%, green anoles extract 0.1-0.5%, dendrobium candidum extract 10-20%, dipotassium glycyrrhizinate 0.1-0.5%, farnesol 0.1-0.2%, hyaluronic acid 0.1-0.5%, glycerol 3-10%, trehalose 0.5-1.5%, p-hydroxyacetophenone 0.1-0.7%, pH regulator 0.1-0.3%, and the rest is deionized water. The ternary compound system of beta-glucan, green anoles extract and dendrobium candidum extract realizes deep water replenishment and barrier repair of the skin cuticle. The antioxidant activity of green anoles is retained, and green anoles, dipotassium glycyrrhizinate and farnesol form an anti-inflammatory soothing system.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a toner rich in water-soluble β-glucan and its preparation method. Background Technology

[0002] High-oil, high-salt diets, wind and sun exposure, stress, and air pollution can all lead to excessive free radical production in the skin. Simultaneously, as people age, the skin's metabolic capacity gradually weakens, the aging process accelerates, and the skin's lipid barrier's water-retaining function declines, resulting in dullness and dehydration. Dehydrated skin can cause a series of skin problems, even damaging the skin barrier and leading to skin diseases. Therefore, moisturizing is essential. Furthermore, moisturizing is often considered the first step in combating skin aging, helping to maintain the skin's appearance and elasticity, and strengthening its barrier function against harmful environmental factors.

[0003] β-glucan, with its unique biological functions and physicochemical properties, such as antioxidant and water-absorbing properties, demonstrates extraordinary value in the skincare field. It also possesses film-forming properties, acting as a second skin: on the one hand, it significantly enhances the skin's moisturizing ability; on the other hand, it reduces the irritation caused by surfactants, gently protecting the skin barrier. Based on this, the present invention aims to provide a toner with safer ingredients and better moisturizing effects, offering consumers more choices. Summary of the Invention

[0004] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the invention.

[0005] In view of the problem of insignificant hydration effect in the above and / or existing technologies, the present invention is proposed.

[0006] Therefore, the purpose of this invention is to overcome the shortcomings of the prior art and provide a cosmetic lotion rich in water-soluble β-glucan.

[0007] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a toner rich in water-soluble β-glucan, comprising, by mass fraction, 0.2-1% β-glucan, 0.1-0.5% *Epipremnum aureum* extract, 10-20% *Dendrobium nobile* extract, 0.1-0.5% dipotassium glycyrrhizate, 0.1-0.2% bisabolol, 0.1-0.5% hyaluronic acid, 3-10% glycerin, 0.5-1.5% trehalose, 0.1-0.7% p-hydroxyacetophenone, 0.1-0.3% pH adjuster, and the balance being deionized water.

[0008] As a preferred embodiment of the water-soluble β-glucan-rich toner of the present invention, wherein the molecular weight of the β-glucan is 10 kDa to 2000 kDa.

[0009] As a preferred embodiment of the water-soluble β-glucan-rich toner of the present invention, the pothos extract includes one or more of pothos leaf extract, pothos root extract, and pothos stem extract.

[0010] As a preferred embodiment of the water-soluble β-glucan-rich cosmetic water of the present invention, wherein: the Dendrobium officinale includes one or more of Dendrobium chrysogenum and Dendrobium officinale produced in Sichuan.

[0011] As a preferred embodiment of the water-soluble β-glucan-rich toner of the present invention, wherein the mass ratio of Dendrobium officinale extract from Sichuan to Dendrobium officinale extract from Sichuan is 6~9.5∶0.5~4.

[0012] As a preferred embodiment of the water-soluble β-glucan-rich toner of the present invention, wherein the mass ratio of Dendrobium officinale extract from Sichuan to Dendrobium officinale extract from Sichuan is 9:1.

[0013] As a preferred embodiment of the toner rich in water-soluble β-glucan described in this invention, the mass ratio of β-glucan, Epipremnum aureum extract and Dendrobium officinale extract is 0.2~1∶0.1~0.5∶15.

[0014] As a preferred embodiment of the toner rich in water-soluble β-glucan described in this invention, the mass ratio of β-glucan, Epipremnum aureum extract, and Dendrobium officinale extract is 0.5:0.2:15.

[0015] Another objective of this invention is to overcome the shortcomings of the prior art and provide a method for preparing a toner rich in water-soluble β-glucan, comprising: weighing each raw material according to the formula, adjusting the pH to 5.5-6.5, homogenizing at 9000-15000 rpm until all components are evenly mixed, and then sterilizing by filtration membrane to obtain a toner rich in water-soluble β-glucan.

[0016] In a preferred embodiment of the preparation method of the water-soluble β-glucan-rich toner of the present invention, the method for preparing the *Epipremnum aureum* extract includes: refluxing the sample with 85-98% ethanol, drying and crushing the *Epipremnum aureum* for 2-4 hours, repeating this process 3-4 times; combining the extracts and drying them; adding the dried product to a citric acid aqueous solution for ultrasonic treatment; washing and concentrating under reduced pressure to obtain powdered *Epipremnum aureum* extract. The concentration of the citric acid aqueous solution is 4-6 wt.%.

[0017] As a preferred embodiment of the preparation method of the water-soluble β-glucan-rich lotion of the present invention, the ultrasonic treatment includes a material-to-liquid ratio of 1g:8~15mL, a frequency of 45kHz, a power of 200W, an intermittent mode of 5s on and 2s off, a temperature of 35~45℃, and a time of 15~25min.

[0018] As a preferred embodiment of the preparation method of the water-soluble β-glucan-rich lotion of the present invention, the preparation method of the Dendrobium officinale extract includes: after crushing Dendrobium officinale, setting the material-to-liquid ratio to 1g:30~50mL, water extraction at 80~100℃ for 40~50min, centrifugation, taking the supernatant, concentrating the supernatant under reduced pressure, adding anhydrous ethanol, precipitating with ethanol for 10~15h, centrifugation, taking the precipitate and drying to obtain Dendrobium officinale extract.

[0019] Beneficial effects of this invention: This invention utilizes a ternary compound system of β-glucan, *Epipremnum aureum* extract, and *Dendrobium nobile* extract to achieve deep hydration, resulting in a dual enhancement of repair and moisturization. Through a citric acid and ultrasound-assisted process, calcium oxalate in *Epipremnum aureum* is broken down, eliminating its irritant properties while preserving its antioxidant activity. Simultaneously, *Epipremnum aureum* forms an anti-inflammatory and soothing system with dipotassium glycyrrhizate and bisabolol.

[0020] This invention innovatively uses Dendrobium officinale, a traditional Chinese medicine, to provide a cosmetic water with safer ingredients and better moisturizing effects, adapting to local conditions for market development. Attached Figure Description

[0021] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein: Figure 1 The graph shows the changes in TEWL values ​​of the lotions prepared in Examples 1-6 and Comparative Examples 1-7.

[0022] Figure 2 The graph shows the changes in TEWL values ​​of the lotions prepared in Examples 1, 7-13 and Comparative Examples 8-10. Detailed Implementation

[0023] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the examples in the specification.

[0024] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0025] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0026] β-glucan is Celec β-glucan produced by Sichuan Hetai Xinguang Biotechnology Co., Ltd.

[0027] The pothos was purchased from Qingbo Flower Market in Qingyang District, Chengdu.

[0028] Dendrobium orchids of different origins and varieties were purchased from the Chengdu Hehuachi Traditional Chinese Medicine Market, with 3-year-old stems.

[0029] Other raw materials were purchased from Sinopharm Chemical Reagent Co., Ltd., and all were of analytical grade.

[0030] Example 1

[0031] This embodiment provides a toner rich in water-soluble β-glucan, specifically: The formula, by mass fraction, includes: 0.5% β-glucan, 0.2% Epipremnum aureum extract, 15% Dendrobium nobile extract, 0.3% dipotassium glycyrrhizate, 0.2% bisabolol, 0.3% hyaluronic acid, 5% glycerin, 1% trehalose, 0.5% p-hydroxyacetophenone, 0.15% pH adjuster (citric acid), and the balance being deionized water.

[0032] Preparation method: 1) Select fresh, intact, and disease-free leaves of pothos, disinfect and clean them, dry and crush them, and extract the sample by reflux with 95% ethanol for 3 hours. Repeat this process 4 times. Combine the extracts and dry them. Add the dried product to a 5 wt.% citric acid aqueous solution and sonicate at 40℃ for 20 minutes. The material-to-liquid ratio is 1 g: 10 mL. The sonication frequency is 45 kHz, the power is 200 W, and the intermittent mode is 5 seconds on and 2 seconds off. After the process, wash three times with deionized water and concentrate under reduced pressure to obtain powdered pothos extract.

[0033] 2) The dried stems of Dendrobium chrysanthum and Dendrobium officinale from Sichuan were crushed separately, and the material-to-liquid ratio was set at 1g:40mL. The mixture was extracted with water at 90℃ for 45min, centrifuged, and the supernatant was collected. The supernatant was concentrated under reduced pressure and anhydrous ethanol was added. The mixture was precipitated with ethanol for 12h, centrifuged and the supernatant was discarded. The precipitate was dried at 65℃ to obtain extracts. The Dendrobium chrysanthum extract and Dendrobium officinale extract were mixed evenly at a mass ratio of 9:1 to obtain Dendrobium chrysanthum extract.

[0034] 3) Weigh each ingredient according to the formula. The mass ratio of β-glucan, ivy extract and dendrobium extract is 0.5:0.2:15. After mixing evenly, adjust the pH to 6 with citric acid, then homogenize at 10,000 rpm for 10 min, and sterilize with a 0.22 μm filter membrane to obtain a toner rich in water-soluble β-glucan.

[0035] Example 2

[0036] The difference between this embodiment and Example 1 is that the mass ratio of β-glucan, ivy extract and dendrobium extract is adjusted to 0.2:0.2:15, and the deionized water content is adjusted simultaneously to ensure that the total mass fraction meets 100%. The rest of the preparation process is the same as in Example 1, and a toner rich in water-soluble β-glucan is obtained.

[0037] Example 3

[0038] The difference between this embodiment and Example 1 is that the mass ratio of β-glucan, ivy extract and dendrobium extract is adjusted to 0.8:0.2:15, and the deionized water content is adjusted simultaneously to ensure that the total mass fraction meets 100%. The rest of the preparation process is the same as in Example 1, and a toner rich in water-soluble β-glucan is obtained.

[0039] Example 4

[0040] The difference between this embodiment and Example 1 is that the mass ratio of β-glucan, ivy extract and dendrobium extract is adjusted to 1:0.2:15, and the deionized water content is adjusted simultaneously to ensure that the total mass fraction meets 100%. The rest of the preparation process is the same as in Example 1, and a toner rich in water-soluble β-glucan is obtained.

[0041] Example 5

[0042] The difference between this embodiment and Example 1 is that the mass ratio of β-glucan, ivy extract and dendrobium extract is adjusted to 0.5:0.1:15, and the deionized water content is adjusted simultaneously to ensure that the total mass fraction meets 100%. The rest of the preparation process is the same as in Example 1, and a toner rich in water-soluble β-glucan is obtained.

[0043] Example 6

[0044] The difference between this embodiment and Example 1 is that the mass ratio of β-glucan, ivy extract and dendrobium extract is adjusted to 0.5:0.5:15, and the deionized water content is adjusted simultaneously to ensure that the total mass fraction meets 100%. The rest of the preparation process is the same as in Example 1, and a toner rich in water-soluble β-glucan is obtained.

[0045] Comparative Example 1

[0046] The difference between this comparative example and Example 1 is that the mass ratio of β-glucan, ivy extract and dendrobium extract was adjusted to 0:0.2:15, and the deionized water content was adjusted simultaneously to ensure that the total mass fraction was 100%. The rest of the preparation process was the same as in Example 1, and a toner rich in water-soluble β-glucan was obtained.

[0047] Comparative Example 2

[0048] The difference between this comparative example and Example 1 is that the mass ratio of β-glucan, ivy extract and dendrobium extract was adjusted to 0.5:0:15, and the deionized water content was adjusted simultaneously to ensure that the total mass fraction was 100%. The rest of the preparation process was the same as in Example 1, and a toner rich in water-soluble β-glucan was obtained.

[0049] Comparative Example 3

[0050] The difference between this comparative example and Example 1 is that the mass ratio of β-glucan, ivy extract and dendrobium extract was adjusted to 0.5:0.2:0, and the deionized water content was adjusted simultaneously to ensure that the total mass fraction was 100%. The rest of the preparation process was the same as in Example 1, and a toner rich in water-soluble β-glucan was obtained.

[0051] Comparative Example 4

[0052] The difference between this comparative example and Example 1 is that the mass ratio of β-glucan, ivy extract and dendrobium extract was adjusted to 0.5:0:0, and the deionized water content was adjusted simultaneously to ensure that the total mass fraction was 100%. The rest of the preparation process was the same as in Example 1, and a toner rich in water-soluble β-glucan was obtained.

[0053] Comparative Example 5

[0054] The difference between this comparative example and Example 1 is that the mass ratio of β-glucan, ivy extract and dendrobium extract was adjusted to 0:0:15, and the deionized water content was adjusted simultaneously to ensure that the total mass fraction was 100%. The rest of the preparation process was the same as in Example 1, and a toner rich in water-soluble β-glucan was obtained.

[0055] Comparative Example 6

[0056] The difference between this comparative example and Example 1 is that the mass ratio of β-glucan, ivy extract and dendrobium extract was adjusted to 0:0.2:0, and the deionized water content was adjusted simultaneously to ensure that the total mass fraction was 100%. The rest of the preparation process was the same as in Example 1, and a toner rich in water-soluble β-glucan was obtained.

[0057] Comparative Example 7

[0058] The difference between this comparative example and Example 1 is that the addition amounts of β-glucan, ivy extract, and dendrobium extract were all adjusted to 0, and the deionized water content was adjusted simultaneously to ensure that the total mass fraction met 100%. The rest of the preparation process was the same as in Example 1, and a toner rich in water-soluble β-glucan was obtained.

[0059] Example 7

[0060] The difference between this embodiment and Example 1 is that the green ivy leaf extract is changed to green ivy root extract, while the rest of the preparation process is the same as in Example 1, resulting in a toner rich in water-soluble β-glucan.

[0061] Example 8

[0062] The difference between this embodiment and Embodiment 1 is that the extract of Epipremnum aureum leaf is changed to the extract of Epipremnum aureum stem, while the rest of the preparation process is the same as in Embodiment 1, to obtain a toner rich in water-soluble β-glucan.

[0063] Example 9

[0064] The difference between this embodiment and Example 1 is that the extraction temperature of Dendrobium officinale was adjusted to 80℃, while the rest of the preparation process was the same as in Example 1, resulting in a cosmetic water rich in water-soluble β-glucan.

[0065] Example 10

[0066] The difference between this embodiment and Example 1 is that the extraction temperature of Dendrobium officinale is adjusted to 100℃, while the rest of the preparation process is the same as in Example 1, resulting in a cosmetic water rich in water-soluble β-glucan.

[0067] Example 11

[0068] The difference between this embodiment and Example 1 is that the mass ratio of Dendrobium nobile extract to Dendrobium officinale extract in the Dendrobium nobile extract is adjusted to 9.5:0.5. The rest of the preparation process is the same as in Example 1, and a toner rich in water-soluble β-glucan is obtained.

[0069] Example 12

[0070] The difference between this embodiment and Example 1 is that the mass ratio of Dendrobium nobile extract to Dendrobium officinale extract in the Dendrobium nobile extract is adjusted to 8:2. The rest of the preparation process is the same as in Example 1, and a toner rich in water-soluble β-glucan is obtained.

[0071] Example 13

[0072] The difference between this embodiment and Example 1 is that the mass ratio of Dendrobium nobile extract to Dendrobium officinale extract in the Dendrobium nobile extract is adjusted to 6:4. The rest of the preparation process is the same as in Example 1, and a toner rich in water-soluble β-glucan is obtained.

[0073] Comparative Example 8

[0074] The difference between this comparative example and Example 1 is that the Dendrobium officinale extract in this example is adjusted to be Dendrobium officinale from Yunnan Province. The rest of the preparation process is the same as in Example 1, and a lotion rich in water-soluble β-glucan is obtained.

[0075] Comparative Example 9

[0076] The difference between this comparative example and Example 1 is that the Dendrobium officinale extract was adjusted to be Dendrobium officinale from Anhui Province. The rest of the preparation process is the same as in Example 1, and a lotion rich in water-soluble β-glucan is obtained.

[0077] Comparative Example 10

[0078] The difference between this comparative example and Example 1 is that the Dendrobium officinale extract was changed to Dendrobium nobile from Guangxi. The rest of the preparation process is the same as in Example 1, and a toner rich in water-soluble β-glucan is obtained.

[0079] The performance of the toners prepared in the above embodiments and comparative examples was tested: 1. The MTT assay was used to test the in vitro cytotoxicity of the toner: HaCaT cells (human immortalized keratinocytes) were prepared into single-cell suspensions and seeded in 96-well plates at a density of 1 × 10⁶ cells / well. 4 Cells per well were incubated at 37°C in a 5% CO2 incubator for 24 hours. Then, 100 μL of ordinary culture medium (control group) and culture medium containing the cosmetic lotions prepared according to the various embodiments and comparative examples of this invention (experimental groups, concentrations of 200, 100, 50, and 25 mg / mL, respectively) were added to each well. Incubation continued for 48 hours. Then, 20 μL of 5 mg / mL MTT solution was added to each well, and after incubation for 4 hours, 150 μL of isopropanol was added to each well. The 96-well plate was gently shaken to ensure uniform reagent distribution, and the absorbance (OD) value was measured at 570 nm. A blank group containing only culture medium and MTT solution was simultaneously set up. Cell viability was calculated for each group based on the OD value using the following formula: Cell viability = 1 - [(OD)] 对照组 -OD 实验组 ) / (OD 对照组 -OD 空白组 )]×100%.

[0080] The results are shown in Table 1.

[0081] Table 1 Comparison of cell viability of HaCaT cells in each group

[0082] The results showed that the toners prepared in the blank control group and the comparative example had no significant effect on HaCaT cell viability, and the cells grew normally. However, the toners prepared in each embodiment of this invention all showed the effect of increasing HaCaT cell viability in vitro.

[0083] 2. The skin safety of the toner was tested using a human patch test: The patch test was conducted according to the general principles of diagnosis and treatment of skin diseases caused by cosmetics (GB17149-1-1997). Thirty eligible subjects were selected. The toners prepared according to the various embodiments and comparative examples of this invention were placed in the patch tester, and 0.02–0.025 mL of sample was added to the filter paper attached to the tester. The patch tester was applied to the inner forearm of the subject using non-irritating adhesive tape, with one side as the control area and the other as the test area. The patch was gently pressed with the palm of the hand to ensure even application to the skin. After 24 hours, the patch tester was removed, and the skin reaction was observed after a 30-minute interval until the pressure marks disappeared. If the result was negative, the skin was observed again at 24 hours and 48 hours after the patch test.

[0084] Record the reaction results according to the skin reaction grading criteria in Table 2. If more than 5 out of 30 subjects have a grade 1 skin adverse reaction, or more than 2 out of 30 subjects have a grade 2 skin adverse reaction, or if any one subject has a grade 3 or higher skin adverse reaction, the test substance is determined to have an adverse skin reaction on the human body.

[0085] Table 2 Grading Criteria for Adverse Skin Reactions in Human Patch Testing

[0086] The results are shown in Table 3.

[0087] Table 3 Results of human skin patch test

[0088] As can be seen from the table above, the toners prepared in the various embodiments and comparative examples of the present invention have no adverse reactions on human skin and have low irritation.

[0089] 3. The moisturizing properties of the toner were tested using the following methods: This experiment simulates the real state of human skin by attaching medical-grade breathable adhesive to a small glass plate. The entire experiment was conducted in a constant temperature and humidity environment: 25°C and 50%RH. First, the weight of the glass plate adhesive tape (M0, g) was measured. Then, 0.5 g of the cosmetic lotion prepared according to the various embodiments and comparative examples of this invention was uniformly coated onto the surface of the simulation device, i.e., the total weight of the glass plate adhesive tape and cosmetic lotion (M1, g). The glass plate coated with cosmetic lotion was then placed in a desiccator containing a saturated CaCl2 solution. After 12 hours, the weight containing the mask tape was measured (M2, g). This was repeated three times, and the average value was calculated.

[0090] The formula for calculating the moisturizing rate W (%) is: Moisturizing rate (%) = (M2 - M1) / (M1 - M0) × 100% Transdermal water loss (TEWL) was measured using the Tewameter™ 300 instrument. Several female volunteers with healthy skin and no history of skin diseases or allergies were selected. Since individual differences in the limbs are relatively small, they are suitable as test sites. An appropriate area of ​​the flexor region of the volunteers' forearms was selected as the test area. Volunteers were allowed to settle down in an environment with a room temperature below 25℃±2℃ and a relative humidity (RH) below 50%±2%. Ten minutes before the test, the test area was exposed, and the TEWL value was measured. The test value before applying toner was used as a control. Toner was applied once with the same application density for a short period, gently patted until absorbed, and the transdermal water loss before and after use was measured.

[0091] The results of the moisturizing performance are shown in the table below: Table 4. Moisturizing effects of different groups of toners

[0092] Based on the table above Figure 1 , Figure 2It can be seen that the in vitro moisturizing rate of the toner prepared by this invention is still greater than 70% after 12 hours, maintaining a high level of moisturizing. The highest moisturizing rate, reaching 87.15%, is achieved when β-glucan, *Epipremnum aureum* extract, and *Dendrobium nobile* extract are combined in the optimal ratio. Meanwhile, based on the changes in transepidermal water loss (TEWL) in volunteers using the toner at different times, it can be seen that all embodiments significantly reduced TEWL, effectively reducing transepidermal water evaporation, which is superior to the comparative example. This indicates that the formula provided by this invention balances hydration and water retention, exhibiting significant barrier repair and moisturizing effects. High-quality moisturizing products can improve the skin's stratum corneum barrier function and reduce transepidermal water loss. Within 0-2 hours of applying the toner prepared in each embodiment, TEWL decreased rapidly and significantly, indicating that the toner quickly forms a moisturizing film and repairs the skin barrier. The significant reduction in TEWL within 2 hours indicates that the toner prepared by our invention can quickly form a moisturizing film on the skin surface, achieving an immediate moisturizing effect; after 8 hours, it still maintains a more than 10% improvement effect. Transepidermal water loss is reduced by more than 20% within 2 hours, and continuous moisturizing occurs for 8 hours.

[0093] As can be seen from Examples 1-6 and Comparative Examples 1-7, adjusting the addition ratio of β-glucan, *Epipremnum aureum* extract, and *Dendrobium nobile* extract significantly affects the performance of the toner. The three exhibit a synergistic effect at specific ratios; however, when the β-glucan content is too high, the moisturizing rate decreases. This may be because high concentrations of polysaccharides lead to excessive system viscosity, affecting the spread and absorption of active ingredients on the skin surface, and also causing a sticky feel that affects film uniformity. According to the results in Table 4, the optimal technical effect is achieved when the mass ratio of β-glucan, *Epipremnum aureum* extract, and *Dendrobium nobile* extract in this invention is 0.5:0.2:15.

[0094] As can be seen from Examples 1, 7, and 8, the moisturizing and repairing effects of the extract from the leaves of *Epipremnum aureum* are superior to those from the roots and stems. Although the roots and stems also have moisturizing effects, the leaves contain richer antioxidant active substances or specific moisturizing factors. The active substances in the leaves are more completely preserved after extraction, and they work better with β-glucan and *Dendrobium nobile* extract.

[0095] As can be seen from Examples 1, 9, and 10, the extraction temperature of Dendrobium officinale affects the moisturizing effect of the toner. This is because extraction may be insufficient at 80℃, resulting in a low dissolution rate of active ingredients; while 100℃ may damage some heat-sensitive active ingredients (such as polysaccharide structures) in Dendrobium officinale, thereby reducing its moisturizing and repairing abilities.

[0096] Analysis of Examples 1 and 11-13, as well as Comparative Examples 8-10, reveals that due to my country's vast territory and diverse soil and climate, the content of effective components in Dendrobium officinale varies among different varieties and origins. This invention, adapting to local conditions, utilizes Dendrobium officinale from Sichuan, which exhibits irreplaceable advantages in this formulation system, developing a toner that combines moisturizing and water-locking properties.

[0097] In summary, this invention achieves deep hydration and a dual enhancement of repair and moisturizing through a ternary compound system of β-glucan, *Epipremnum aureum* extract, and *Dendrobium nobile* extract. The calcium oxalate in *Epipremnum aureum* is decomposed using citric acid and ultrasound-assisted processes, eliminating its irritant properties while preserving its antioxidant activity. Simultaneously, *Epipremnum aureum* forms an anti-inflammatory and soothing system with dipotassium glycyrrhizate and bisabolol.

[0098] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A toner rich in water-soluble β-glucan, characterized in that: Based on the mass fraction of the said toner, it includes, β-glucan 0.2-1%, Epipremnum aureum extract 0.1-0.5%, Dendrobium nobile extract 10-20%, dipotassium glycyrrhizate 0.1-0.5%, bisabolol 0.1-0.2%, hyaluronic acid 0.1-0.5%, glycerin 3-10%, trehalose 0.5-1.5%, p-hydroxyacetophenone 0.1-0.7%, pH adjuster 0.1-0.3%, balance deionized water; The mass ratio of β-glucan, Epipremnum aureum extract and Dendrobium officinale extract is 0.2~1:0.1~0.5:15; in the Dendrobium officinale extract, the mass ratio of Dendrobium chrysogenum extract from Sichuan to Dendrobium officinale extract from Sichuan is 6~9.5:0.5~4.

2. The toner rich in water-soluble β-glucan as described in claim 1, characterized in that: The molecular weight of the β-glucan is 10 kDa to 2000 kDa.

3. The toner rich in water-soluble β-glucan as described in claim 2, characterized in that: The pothos extract includes one or more of the following: pothos leaf extract, pothos root extract, and pothos stem extract.

4. The facial toner rich in water-soluble β-glucan as described in claim 3, characterized in that: The pH adjuster includes one or more of citric acid, sodium lactate, sodium hydroxide, potassium hydroxide, triethanolamine, aminomethylpropanol, and arginine.

5. The facial toner rich in water-soluble β-glucan as described in claim 1, characterized in that: The mass ratio of β-glucan, Epipremnum aureum extract, and Dendrobium nobile extract is 0.5:0.2:

15.

6. The method for preparing a facial toner rich in water-soluble β-glucan as described in any one of claims 1 to 5, characterized in that: include, Weigh each ingredient according to the formula, adjust the pH to 5.5~6.5, homogenize at 9000~15000 rpm until all components are evenly mixed, and then sterilize by filtration membrane to obtain a toner rich in water-soluble β-glucan.

7. The method for preparing a facial toner rich in water-soluble β-glucan as described in claim 6, characterized in that: The preparation method of the green ivy extract, include, Extract the sample by reflux with 85-98% ethanol and dry the crushed pothos for 2-4 hours. Repeat this process 3-4 times. Combine the extracts and dry them. Add the dried product to a citric acid aqueous solution for ultrasonic treatment. After washing and vacuum concentration, obtain powdered pothos extract. The concentration of the citric acid aqueous solution is 4-6 wt%.

8. The method for preparing a facial toner rich in water-soluble β-glucan as described in claim 7, characterized in that: The ultrasonic treatment includes a material-to-liquid ratio of 1g:8~15mL, a frequency of 45kHz, a power of 200W, an intermittent mode of 5s on and 2s off, a temperature of 35~45℃, and a time of 15~25min.

9. The method for preparing a facial toner rich in water-soluble β-glucan as described in claim 8, characterized in that: Preparation method of Dendrobium nobile extract include, After crushing Dendrobium nobile, the material-to-liquid ratio was set to 1g:30~50mL, and water extraction was performed at 80~100℃ for 40~50min. After centrifugation, the supernatant was collected, concentrated under reduced pressure, and then anhydrous ethanol was added. After ethanol precipitation for 10~15h, the precipitate was centrifuged and dried to obtain Dendrobium nobile extract.

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