A composition with whitening and freckle-removing effects, and a preparation method and application thereof

Abstract

The application discloses a composition with whitening and freckle-removing effects, a preparation method and application thereof, and belongs to the technical field of compositions. The composition provided by the application reduces the oxidation stress level of a body, provides a suitable internal microenvironment for the exertion of various pharmacological effects and the repair of damage, provides sufficient, extremely rich in types and easy-to-absorb and utilize material basis for the repair of skin damage, greatly improves the whitening and freckle-removing effects by improving the activity, purity and content of components directly having the whitening and freckle-removing effects, promotes the discharge of metabolic waste by improving the gastrointestinal health, is extremely safe, has low equipment requirements, is environment-friendly, and can be used for daily skin repair, whitening and freckle-removing.

Description

Technical Field

[0001] This invention relates to the field of composition technology, and in particular to a composition with whitening and freckle-removing effects, its preparation method, and its application. Background Technology

[0002] The core reason for skin darkening and the appearance of spots is that melanocytes are stimulated to secrete melanin at an excessive rate. The secreted melanin accumulates evenly on the skin's surface, causing the skin to darken. If the produced melanin gathers in a localized area of ​​the skin, it forms patches (such as freckles, melasma, age spots, sun spots, and pigmentation spots).

[0003] The primary external cause of skin darkening and pigmentation is ultraviolet radiation. UVA and UVB rays in sunlight can penetrate the skin and directly activate melanocytes. To prevent sunburn and DNA damage, the body automatically synthesizes large amounts of melanin. Secondly, factors such as staying up late, high stress, poor mood, hormonal imbalances during special periods (e.g., pregnancy, menopause), and unhealthy lifestyle habits like smoking and drinking can lead to cortisol imbalances that stimulate melanin production. Slower metabolism prevents timely elimination of waste products, leading to the accumulation of harmful substances. A weakened immune system also makes the skin more prone to darkening and dullness. Glycation caused by a high-sugar diet can also result in yellowing and dullness. Furthermore, acne, allergies, scratching or abrasions that damage the skin's surface, cosmetic procedures that break the skin, and the healing of skin inflammation can all leave brownish discoloration. Natural aging and genetic factors also increase the likelihood of skin darkening and pigmentation.

[0004] Darkening and / or the appearance of age spots not only affect appearance and self-confidence but also pose potential health risks. Darkened and spotted skin often has a fragile skin barrier and poor moisture retention, making it prone to sensitivity, redness, dryness, itching, and recurring allergies. This indicates ongoing damage to the skin barrier, collagen loss, and elastin degradation. These problems accelerate skin aging, leading to more severe skin barrier damage, which in turn worsens or even spreads age spots, creating a vicious cycle. Furthermore, persistent darkening and age spots may be a distress signal from the body, indicating underlying issues such as sleep deprivation, endocrine disorders, liver qi stagnation, and impaired metabolism.

[0005] The common method of covering up with makeup cannot truly solve the problem. Only by addressing the root cause and regulating one's constitution can the problem be improved / solved from its source. Summary of the Invention

[0006] The purpose of this invention is to provide a composition with whitening and freckle-removing effects, its preparation method and application, so as to provide a composition with good whitening and freckle-removing effects, high safety, low irritation and side effects, wide applicability, and can promote the formation of a virtuous cycle in the body, and has the potential to promote the whitening and freckle-removing of the body's skin from the root.

[0007] To achieve the above objectives, the present invention provides a composition with whitening and freckle-removing effects, the composition comprising the following components in parts by weight: 5-10 parts Angelica dahurica, 10-20 parts Polygonatum odoratum, 10-20 parts mung bean powder, 5-10 parts winter melon seed kernels and 10-20 parts shiitake mushroom powder.

[0008] Preferably, the mung bean powder is obtained by grinding fresh dried mung beans and passing them through a 60-80 mesh sieve; the Solomon's seal is harvested in autumn; and the shiitake mushroom powder is obtained by removing impurities from fresh shiitake mushrooms, freeze-drying them, pulverizing them, and then passing them through a 60-80 mesh sieve.

[0009] Preferably, the composition comprises the following components in parts by weight: 8 parts Angelica dahurica, 12 parts Polygonatum odoratum, 15 parts mung bean powder, 7 parts winter melon seed kernels, and 15 parts shiitake mushroom powder.

[0010] Preferably, the composition comprises the following components in parts by weight: 5 parts Angelica dahurica, 10 parts Polygonatum odoratum, 10 parts mung bean powder, 5 parts winter melon seed kernels, and 10 parts shiitake mushroom powder.

[0011] Preferably, the composition comprises the following components in parts by weight: 10 parts Angelica dahurica, 20 parts Polygonatum odoratum, 20 parts mung bean powder, 10 parts winter melon seed kernels, and 20 parts shiitake mushroom powder.

[0012] A method for preparing a composition with whitening and freckle-removing effects as described above, comprising the following steps: S1. Grind Angelica dahurica, Polygonatum odoratum and winter melon seeds into powder, and pass them through a 60-80 mesh sieve to obtain Angelica dahurica powder, Polygonatum odoratum powder and winter melon seed powder respectively. S2. Add angelica powder, polygonatum powder and shiitake powder to an opaque bottle, then add sterile drinking water, stir and mix well, let stand at room temperature, then incubate at 18-22℃ and 180-200rpm for 24-72 hours, then seal with a sterile air-impermeable membrane and let stand. S3. After adjusting the pH of the mixture obtained in S2, add Bacillus subtilis and incubate at 180-200 rpm for 24-72 hours. Then adjust the pH again, add mung bean powder and winter melon seed powder, mix well and incubate at 180-200 rpm for 24-48 hours. S4. Filter the culture obtained in S3, and spray-dry the filtrate.

[0013] Preferably, the amount of sterile drinking water added in S2 is 10-15 times the total mass of Angelica dahurica powder, Polygonatum odoratum powder, mung bean powder, winter melon seed powder and shiitake mushroom powder.

[0014] Preferably, after adding sterile drinking water to S2 and mixing it, the mixture is left to stand at room temperature for 30-60 minutes, and mixed once during this period; after sealing with a sterile, airtight membrane, the mixture is left to stand at 18-22°C for 36-72 hours.

[0015] Preferably, in step S3, pH adjustment is performed using 1M HCl and 1M NaOH to adjust the pH to 6.5-7.0; in step S4, the inlet air temperature for spray drying is 130-200℃, and the outlet air temperature is 60-80℃.

[0016] Angelica dahurica ( Angelica dahurica (Fisch. ex Hoffm.) Benth. & Hook.f. ex Franch. & Sav.) It has a pungent taste and warm nature, and is good at dispersing and penetrating deep into the Yin and Yang meridians (Stomach and Large Intestine meridians) of the body. Traditional Chinese medicine uses it to dispel wind and relieve pain, clear nasal passages, dry dampness and stop leukorrhea, and reduce swelling and drain pus. Studies have shown that Angelica dahurica has the effects of soothing the liver and regulating Qi, dispelling dampness and detoxifying. By regulating endocrine function, it reduces the formation of pigmentation from within, brightens skin tone, and improves dullness, yellowing, dark spots, and melasma.

[0017] The core active ingredients in Angelica dahurica are coumarins (including imperatorin, isoimperatorin, oxyimperatorin, bergamot lactone, and sennain), which have anti-inflammatory, analgesic, antibacterial, antioxidant, antitumor, and whitening effects; volatile oils (α-pinene, camphene, limonene, etc.) provide a unique aroma and have deodorizing, flavor-enhancing, antibacterial, and anti-inflammatory effects; it also contains polysaccharides, alkaloids, flavonoids, ferulic acid, sterols, etc., which are effective in immune regulation, free radical scavenging, and anti-aging. It also contains carbohydrates (accounting for more than 60% of the total weight), protein, dietary fiber, minerals (including calcium, iron, zinc, etc.), and various vitamins (including vitamins B1, B2, E, etc.).

[0018] However, Angelica dahurica is pungent, warm, and drying in nature. Long-term or frequent consumption of its decoction can easily deplete yin and fluids, leading to symptoms of internal heat such as dry mouth, dry throat, and chapped lips. It can also slightly deplete vital energy, making it unsuitable for people with yin deficiency, qi deficiency, or weak constitutions, as it may worsen their symptoms. Furthermore, Angelica dahurica is irritating to the stomach and intestines; it should not be consumed by those with stomach coldness, acid reflux, gastritis, gastric ulcers, or on an empty stomach. Studies have also shown that long-term consumption can disrupt the body's yin-yang balance, leading to endocrine disorders and exacerbating yellowing skin and acne due to internal dryness. In addition, Angelica dahurica contains phototoxic furanocoumarins, which can cause skin cell damage and death under ultraviolet radiation, posing a major safety risk.

[0019] Polygonatum odoratum scientific name Polygonatum odoratum(Mill.) Druce, also known as tail ginseng, ground tube, bell vegetable, and so on, is a perennial herb belonging to the genus Polygonatum in the family Asparagaceae. Polygonatum odoratum is a plant used both as food and medicine. According to the Chinese Pharmacopoeia, Polygonatum odoratum is sweet in taste and slightly cold in nature, entering the lung and stomach meridians. It nourishes yin and moistens dryness, promotes body fluid production and quenches thirst, and is used for lung and stomach yin deficiency, dry cough, dry throat and mouth, and internal heat and thirst. Studies have shown that the bioactive components in Polygonatum odoratum can be mainly divided into the following categories: polysaccharides, which are the most abundant and most studied core components, have the ability to enhance the proliferation of B lymphocytes and T lymphocytes, scavenge free radicals, reduce oxidative damage, improve the body's ability to metabolize glucose and lipids, and help regulate blood sugar and blood lipids; steroidal saponins, another important group of active ingredients in Polygonatum odoratum, have various pharmacological effects such as anti-tumor, anti-inflammatory, and hypoglycemic effects; high isoflavones, which have strong antibacterial, antiviral, and antitumor activities; and also include lectins that have inhibitory effects on specific tumor cells, phenylethyl cinnamate with α-glucosidase inhibitory activity, as well as alkaloids and volatile oils.

[0020] Besides its medicinal properties and active ingredients, Solomon's Seal contains abundant basic nutrients, with carbohydrates being the most abundant (soluble sugars account for over 43%, and starch for about 8-15%). It also includes a rich variety of vitamins and minerals, proteins, and amino acids (including many essential amino acids). However, it also contains certain amounts of anti-nutritional components such as lectins, tannins, and oxalic acid.

[0021] This product is not suitable for those with spleen and stomach deficiency, phlegm-dampness stagnation, or those in the early stages of a wind-cold cold. Consuming it directly by boiling or using it alone may irritate the oral and gastrointestinal mucosa, leading to symptoms such as heaviness in the head, loss of appetite, abdominal distension, and diarrhea. It may also cause an imbalance of yin and yang, increasing the burden on the liver and kidneys, and causing allergic reactions such as itchy skin and rashes. Furthermore, short-time boiling will result in insufficient dissolution of the active ingredients, while prolonged boiling will destroy some heat-sensitive active ingredients.

[0022] green beans( Radiant vine (L.) R. Wilczek) is the dried seed of *Phaseolus radiatus* L., belonging to the genus *Phaseolus* of the legume family. It is sweet and cold in nature, primarily affecting the heart and stomach meridians. Its core functions are clearing heat and detoxifying, relieving summer heat, and promoting diuresis. It is mainly used to treat summer heat and thirst, erysipelas, carbuncles, edema, diarrhea, and food and drug poisoning. Mung beans possess both medicinal properties and rich nutritional value, making them a common food in daily life. Mung beans are rich in high-quality plant protein (rich in lysine), dietary fiber, various vitamins (including vitamin E, vitamin B1, vitamin B2, niacin, and vitamin C), and minerals (including potassium, phosphorus, magnesium, calcium, iron, and zinc). Furthermore, the mung bean skin contains polyphenols, flavonoids / flavonoids (almost entirely vitexin and isovitexin), tannins, and other bioactive components.

[0023] Mung beans contain anti-nutritional components such as protease inhibitors, oxalic acid / phytic acid, lectins, and saponins. Their abundant dietary fiber and oligosaccharides easily ferment in the intestines, producing gas, leading to bloating, flatulence, and increased gastrointestinal burden. When consuming mung beans by simmering or boiling, insufficient simmering time can leave residual anti-nutritional factors, also easily causing bloating and diarrhea. Conversely, excessive simmering time for a soft texture results in the loss of significant amounts of vitamins and antioxidants. Furthermore, mung beans can interact with certain medications (such as ginseng, cinnamon, and antibiotics), reducing their efficacy. In addition, people with weak spleen and stomach, kidney insufficiency, and women during menstruation should avoid consuming mung beans, and pregnant women should use them with caution.

[0024] Winter melon ( Benincasa hispida (Thunb.) Cogn.) kernels are cool in nature and sweet in taste, possessing the effects of clearing the lungs and resolving phlegm, eliminating carbuncles and draining pus, and promoting diuresis. They have high nutritional value, including abundant dietary fiber, protein (containing 16 amino acids, including 8 essential amino acids), approximately 20% fat by dry weight (mainly unsaturated fatty acids), and various trace elements (rich in potassium, calcium, magnesium, iron, etc.). They also contain other active ingredients, including alkaloids (such as trigonelline), sphingomyelin, and cerebrosides. Modern research has found that they possess antioxidant, anti-inflammatory, antitumor, and certain antibacterial effects.

[0025] Winter melon seeds are cooling in nature and contain saponins and trypsin inhibitors, so those with weak spleen and stomach should consume them with caution. Furthermore, studies have shown that winter melon seeds can interact with vitamin K, so the two should not be eaten together.

[0026] mushroom( Lentil plant Shiitake mushrooms (Berk.) Pegler are the world's second largest mushroom and a renowned and precious edible fungus in my country. They are also a famous medicinal fungus, with a sweet and neutral flavor, entering the liver and stomach meridians. Their main functions include strengthening the body, invigorating the spleen and stomach, resolving phlegm and regulating qi, dispelling wind and promoting rash eruption, and detoxifying and fighting cancer. The bioactive components of shiitake mushrooms mainly include lentinan, lentinan (also known as "lentinan"), ergosterol, and dietary fiber. Lentinan is the core active ingredient in shiitake mushrooms, possessing various physiological activities such as immunomodulation, anti-tumor, antioxidant, and antiviral effects. It can activate macrophages and T lymphocytes, enhancing immunity. Lentinan has been proven to have a significant cholesterol-lowering effect, helping to maintain cardiovascular health. Ergosterol is an important precursor to vitamin D2, which can be converted into vitamin D2 under ultraviolet radiation, beneficial for promoting calcium absorption and preventing rickets and osteoporosis. The abundant dietary fiber (containing chitin, etc.) is not only a nutrient but also has the activity of regulating intestinal function.

[0027] Shiitake mushrooms are also rich in basic nutrients. Their protein contains 18 kinds of amino acids, including 8 essential amino acids; they contain unsaturated fatty acids such as linoleic acid and oleic acid; various vitamins (including vitamins B1, B2, nicotinic acid, etc.) and minerals, including calcium, phosphorus, iron, potassium, zinc, manganese and other trace elements. The iron content is more than 3 times that of spinach.

[0028] However, the chitin and fungal polysaccharides in shiitake mushrooms are difficult for the human body to digest and can irritate the stomach and intestines; shiitake mushrooms produce trace amounts of formaldehyde during normal metabolism; they are high in purines and should be avoided by gout patients; their rich dietary fiber is not suitable for people with weak digestive function and can cause bloating, abdominal pain and other discomfort.

[0029] Protease inhibitors bind to digestive enzymes in the intestine (such as trypsin), reducing their activity and decreasing the body's ability to digest and utilize protein. Phytic acid / oxalic acid are powerful chelating agents that bind to mineral ions such as calcium, iron, zinc, and magnesium, forming complexes that are difficult for the body to absorb and utilize, thus affecting the body's absorption and utilization of these key minerals. Lectins are proteins that can bind to sugar molecules on cell surfaces, resisting hydrolysis by digestive enzymes in the digestive tract and potentially damaging the small intestinal mucosa and interfering with nutrient absorption. Saponins may bind to substances such as cholesterol, hindering the absorption of nutrients.

[0030] This invention employs a specific fermentation method. On one hand, it decomposes the cellulose in each component into short-chain fatty acids that can be absorbed and utilized by the human body, and degrades the large-molecule proteins into small peptides and amino acids that are more easily absorbed and utilized by the body. This effectively reduces the burden on the gastrointestinal tract, minimizes irritation, and provides a milder eating experience, promoting the absorption and utilization of nutrients while improving intestinal health. On the other hand, the preparation method provided by this invention, by degrading chelates / conjugates such as small peptides and free sugars that are bound to the original active ingredients in each component, can improve the purity and release amount of the original bioactive substances in each component. Furthermore, under the action of the original symbiotic bacteria and Bacillus subtilis in each component, new, more active, and more easily absorbed and utilized active ingredients may be generated. In the efficacy test, the effect in Example 1 was significantly enhanced compared to the effect in Comparative Example 2, which also proves from another perspective that this principle does indeed exist.

[0031] Furthermore, the specific fermentation method provided by this invention can effectively degrade oxalic acid needle crystals contained in raw materials, or harmonize the warm, dry, cold, and cooling properties of each component due to the degradation of specific components, making the composition more mild in effect, less irritating and with fewer side effects, applicable to a wider range of people, and with fewer contraindications.

[0032] In addition, the preparation method provided by the present invention is mild and light-protected, which can retain the heat-sensitive and photosensitive active ingredients in each component. Through the action of probiotics and symbiotic bacteria of raw materials, the various vitamins and minerals are transformed into active or more easily absorbed and utilized forms by the human body.

[0033] Therefore, the present invention provides a composition with whitening and freckle-removing effects, its preparation method, and its application, the specific technical effects of which are as follows: (1) The composition provided by the present invention first reduces dryness through Angelica dahurica and mung bean, and then reduces the oxidative stress level of the body under the anti-inflammatory and antioxidant functions of Angelica dahurica, Polygonatum odoratum and shiitake mushroom, as well as the heat-clearing and detoxifying effect of mung bean, so as to provide a suitable internal microenvironment for the exertion of various drug effects and the repair of damage; (2) The composition provided by the present invention, by resisting the degradation of nutrients and macromolecular nutrients, and by the integration and utilization of vitamins and trace elements by symbiotic bacteria contained in probiotics and raw materials, obtains a large number of small molecule nutrients that can be directly absorbed and utilized by the body, providing a sufficient and extremely rich material basis for the repair of skin damage. (3) The composition provided by the present invention can significantly improve the whitening and spot-removing effect by increasing the activity, purity and content of the components that directly have the whitening and spot-removing effect; by setting the conditions of each step, bacteria, pathogens and symbiotic bacteria of the raw materials can be killed, while the probiotics - Bacillus subtilis (dormant endospores) can be retained in the composition. Then, under the action of short-chain fatty acids and probiotics in the components, the gastrointestinal health is improved, and under the diuretic effect of mung beans, the excretion of metabolic waste is promoted; the large amount of potassium ions contained therein also has the function of regulating blood pressure and maintaining fluid balance, so that the whitening and spot-removing process of the body forms a virtuous cycle; (4) The composition provided by the present invention does not use organic reagents / toxic reagents, and all raw materials are food and medicine materials or commonly used Chinese medicines. It does not contain animal ingredients, has no risk of immunogenicity, etc., and is extremely safe. The preparation conditions are mild and the equipment requirements are low. No toxic or harmful substances are generated, and it is environmentally friendly. It can be used for daily skin repair, whitening and freckle removal.

[0034] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0035] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments of the present invention will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0036] Figure 1 This is the statistical result of the change rate of the proportion of facial pigmentation area in each group of volunteers in the effect test section; where ** indicates the comparison with Example 2, Comparative Example 1 and Comparative Example 2. P <0.01. Detailed Implementation

[0037] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0038] To make the objectives, technical solutions, and advantages of this application clearer, more thorough, and more complete, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings and embodiments. The following detailed descriptions are all illustrations of embodiments, intended to provide further detailed explanation of the present invention. Unless otherwise specified, all technical terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0039] The instruments, equipment, reagents and materials used in the embodiments were all obtained through commercial means; the methods and steps not described in detail in the embodiments are all conventional techniques in the art.

[0040] Example 1 The specific steps for preparing a composition with whitening and freckle-removing effects are as follows: S1. Remove impurities from the purchased mung beans, sliced ​​angelica root, sliced ​​Solomon's seal rhizome harvested in autumn, and winter melon seeds, then crush and grind them into powder, and pass them through a 60-mesh sieve to obtain mung bean powder, angelica root powder, Solomon's seal rhizome powder, and winter melon seed powder, respectively.

[0041] S2. After removing impurities such as culture medium and plastic film from the purchased fresh shiitake mushrooms, clean them, freeze-dry them, then crush and grind them into powder and pass them through a 60-mesh sieve to obtain shiitake mushroom powder.

[0042] S3. Take 8g of Angelica dahurica powder, 12g of Polygonatum odoratum powder, and 15g of Lentinus edodes powder prepared in step S1, respectively, and add them to a brown bottle. Then add 600g of sterile drinking water, stir and mix well, seal with a sterile breathable membrane, and let stand at room temperature for 20 minutes. Then invert and mix well, let stand at room temperature for another 20 minutes, invert and mix well again, and then place the mixture in a 20℃, 180rpm environment for 48 hours. After that, seal with a sterile impermeable membrane and let stand at 20℃ for 48 hours.

[0043] S4. In a clean bench (protected from light), adjust the pH of the mixture after settling in step S3 to 6.8 using 1M HCl and 1M NaOH. Then add Bacillus subtilis SNZ 1972 to bring the concentration of Bacillus subtilis in the mixture to 10. 6 -10 7The cfu / mL sample was sealed with a sterile breathable membrane and incubated at 37°C and 200 rpm for 48 hours.

[0044] S5. Place the culture obtained in S4 in a clean bench (protected from light), and adjust the pH to 6.8 again with 1M HCl and 1M NaOH at 37℃. Then add 15g of mung bean powder and 7g of winter melon seed powder, stir and mix well, and continue to culture at 37℃ and 200rpm for 24 hours.

[0045] S6. Filter the culture obtained in step S5 through a 60-mesh sieve. Spray dry the filtrate that has passed through the sieve, setting the inlet air temperature to 150℃ and the outlet air temperature to 60-80℃. This yields a composition with whitening and freckle-removing effects, totaling 59.08g.

[0046] Example 2 The specific steps for preparing a composition with whitening and freckle-removing effects are as follows: S1. Remove impurities from the purchased mung beans, sliced ​​angelica root, sliced ​​Solomon's seal rhizome harvested in autumn, and winter melon seeds, then crush and grind them into powder, and pass them through a 60-mesh sieve to obtain mung bean powder, angelica root powder, Solomon's seal rhizome powder, and winter melon seed powder, respectively.

[0047] S2. After removing impurities such as culture medium and plastic film from the purchased fresh shiitake mushrooms, clean them, freeze-dry them, then crush and grind them into powder and pass them through a 60-mesh sieve to obtain shiitake mushroom powder.

[0048] S3. Take 8g of Angelica dahurica powder, 12g of Polygonatum odoratum powder, and 15g of Lentinus edodes powder prepared in step S1, respectively, and add them to a brown bottle. Then add 600g of sterile drinking water, seal with a sterile breathable membrane, and let stand at room temperature for 20 minutes. Then invert and mix, let stand at room temperature for another 20 minutes, invert and mix again, and then incubate the mixture at 20℃ and 180rpm for 24 hours. After that, seal with a sterile impermeable membrane and let stand at 20℃ for 24 hours.

[0049] S4. In a clean bench (protected from light), adjust the pH of the mixture after settling in step S3 to 6.8 using 1M HCl and 1M NaOH. Then add Bacillus subtilis SNZ 1972 to bring the concentration of Bacillus subtilis in the mixture to 10. 6 -10 7 The cfu / mL sample was sealed with a sterile breathable membrane and incubated at 37°C and 200 rpm for 48 hours.

[0050] S5. Place the culture obtained in S4 in a clean bench (protected from light), and adjust the pH to 6.8 again with 1M HCl and 1M NaOH at 37℃. Then add 15g of mung bean powder and 7g of winter melon seed powder, stir and mix well, and continue to culture at 37℃ and 200rpm for 24 hours.

[0051] S6. Filter the culture obtained in step S5 through a 60-mesh sieve, and spray-dry the filtrate that has passed through the sieve. Set the inlet air temperature to 150℃ and the outlet air temperature to 60-80℃. This yields a composition with whitening and freckle-removing effects.

[0052] Example 3 The specific steps for preparing a composition with whitening and freckle-removing effects are as follows: S1. Remove impurities from the purchased mung beans, sliced ​​angelica root, sliced ​​Solomon's seal rhizome harvested in autumn, and winter melon seeds, then crush and grind them into powder, and pass them through a 60-mesh sieve to obtain mung bean powder, angelica root powder, Solomon's seal rhizome powder, and winter melon seed powder, respectively.

[0053] S2. After removing impurities such as culture medium and plastic film from the purchased fresh shiitake mushrooms, clean them, freeze-dry them, then crush and grind them into powder and pass them through a 60-mesh sieve to obtain shiitake mushroom powder.

[0054] S3. Take 5g of Angelica dahurica powder, 10g of Polygonatum odoratum powder and 10g of Lentinus edodes powder prepared in step S1 and add them to a brown bottle. Then add 450g of sterile drinking water, seal with a sterile breathable membrane and let stand at room temperature for 20 minutes. Then invert and mix, let stand at room temperature for 48 minutes, invert and mix again, and then incubate the mixture at 20℃ and 180rpm for 24 hours. After that, seal with a sterile impermeable membrane and let stand at 20℃ for 48 hours.

[0055] S4. In a clean bench (protected from light), adjust the pH of the mixture after settling in step S3 to 6.8 using 1M HCl and 1M NaOH. Then add Bacillus subtilis SNZ 1972 to bring the concentration of Bacillus subtilis in the mixture to 10. 6 -10 7 The cfu / mL sample was sealed with a sterile breathable membrane and incubated at 37°C and 200 rpm for 48 hours.

[0056] S5. Place the culture obtained in S4 in a clean bench (protected from light), and adjust the pH to 6.8 again with 1M HCl and 1M NaOH at 37℃. Then add 10g of mung bean powder and 5g of winter melon seed powder, stir and mix well, and continue to culture at 37℃ and 200rpm for 24 hours.

[0057] S6. Filter the culture obtained in step S5 through a 60-mesh sieve, and spray-dry the filtrate that has passed through the sieve. Set the inlet air temperature to 150℃ and the outlet air temperature to 60-80℃. This yields a composition with whitening and freckle-removing effects.

[0058] Example 4 The specific steps for preparing a composition with whitening and freckle-removing effects are as follows: S1. Remove impurities from the purchased mung beans, sliced ​​angelica root, sliced ​​Solomon's seal rhizome harvested in autumn, and winter melon seeds, then crush and grind them into powder, and pass them through a 60-mesh sieve to obtain mung bean powder, angelica root powder, Solomon's seal rhizome powder, and winter melon seed powder, respectively.

[0059] S2. After removing impurities such as culture medium and plastic film from the purchased fresh shiitake mushrooms, clean them, freeze-dry them, then crush and grind them into powder and pass them through a 60-mesh sieve to obtain shiitake mushroom powder.

[0060] S3. Take 10g of Angelica dahurica powder, 20g of Polygonatum odoratum powder, and 20g of Lentinus edodes powder prepared in step S1, respectively, and add them to a brown bottle. Then add 850g of sterile drinking water, stir and mix well, seal with a sterile breathable membrane, and let stand at room temperature for 20 minutes. Then invert and mix well, let stand at room temperature for another 20 minutes, invert and mix well again, and then place the mixture in a 20℃, 180rpm environment for 48 hours. After that, seal with a sterile impermeable membrane and let stand at 20℃ for 48 hours.

[0061] S4. In a clean bench (protected from light), adjust the pH of the mixture after settling in step S3 to 6.8 using 1M HCl and 1M NaOH. Then add Bacillus subtilis SNZ 1972 to bring the concentration of Bacillus subtilis in the mixture to 10. 6 -10 7 The cfu / mL sample was sealed with a sterile breathable membrane and incubated at 37°C and 200 rpm for 48 hours.

[0062] S5. Place the culture obtained in S4 in a clean bench (protected from light), and adjust the pH to 6.8 again with 1M HCl and 1M NaOH at 37℃. Then add 20g of mung bean powder and 10g of winter melon seed powder, stir and mix well, and continue to culture at 37℃ and 200rpm for 24 hours.

[0063] S6. Filter the culture obtained in step S5 through a 60-mesh sieve, and spray-dry the filtrate that has passed through the sieve. Set the inlet air temperature to 150℃ and the outlet air temperature to 60-80℃. This yields a composition with whitening and freckle-removing effects.

[0064] Comparative Example 1 The specific steps for preparing a composition are as follows: S1. Remove impurities from the purchased mung beans, sliced ​​angelica root, sliced ​​Solomon's seal rhizome harvested in autumn, and winter melon seeds, then crush and grind them into powder, and pass them through a 60-mesh sieve to obtain mung bean powder, angelica root powder, Solomon's seal rhizome powder, and winter melon seed powder, respectively.

[0065] S2. After removing impurities such as culture medium and plastic film from the purchased fresh shiitake mushrooms, clean them, freeze-dry them, then crush and grind them into powder and pass them through a 60-mesh sieve to obtain shiitake mushroom powder.

[0066] S3. Take 15g of mung bean powder, 8g of angelica powder, 12g of Solomon's seal powder, 7g of winter melon seed powder, and 15g of shiitake mushroom powder prepared in step S1, respectively, and add them to a brown bottle. Then add 600g of sterile drinking water, seal with a sterile breathable membrane, and let stand at room temperature for 20 minutes. Then invert and mix, let stand at room temperature for another 20 minutes, invert and mix again, and then incubate the mixture at 20℃ and 180rpm for 48 hours. After that, seal with a sterile impermeable membrane and let stand at 20℃ for 48 hours.

[0067] S4. In a clean bench (protected from light), adjust the pH of the mixture after settling in step S3 to 6.8 using 1M HCl and 1M NaOH. Then add Bacillus subtilis SNZ 1972 to bring the concentration of Bacillus subtilis in the mixture to 10. 6 -10 7 The cfu / mL solution was sealed with a sterile breathable membrane and incubated at 37°C and 200 rpm for 72 hours.

[0068] S6. Filter the culture obtained in step S4 through a 60-mesh sieve, and spray-dry the filtrate that has passed through the sieve. Set the inlet air temperature to 150℃ and the outlet air temperature to 60-80℃. This yields a composition with whitening and freckle-removing effects.

[0069] Comparative Example 2 The specific steps for preparing a composition with whitening and freckle-removing effects are as follows: S1. Remove impurities from the purchased mung beans, sliced ​​angelica root, sliced ​​Solomon's seal rhizome harvested in autumn, and winter melon seeds, then crush and grind them into powder, and pass them through a 60-mesh sieve to obtain mung bean powder, angelica root powder, Solomon's seal rhizome powder, and winter melon seed powder, respectively.

[0070] S2. After removing impurities such as culture medium and plastic film from the purchased fresh shiitake mushrooms, clean them, freeze-dry them, then crush and grind them into powder and pass them through a 60-mesh sieve to obtain shiitake mushroom powder.

[0071] S3. Take 8g of Angelica dahurica powder, 12g of Polygonatum odoratum powder and 15g of shiitake mushroom powder prepared in step S1 respectively, add them to a brown bottle, then add 600g of sterile drinking water, stir for 20 minutes to form a suspension.

[0072] S4. In a clean bench (protected from light), adjust the pH of the suspension obtained in step S3 to 6.8 using 1M HCl and 1M NaOH. Then add Bacillus subtilis SNZ 1972 to the mixture to achieve a concentration of Bacillus subtilis of 10%. 6 -10 7 The cfu / mL sample was sealed with a sterile breathable membrane and incubated at 37°C and 200 rpm for 48 hours.

[0073] S5. Place the culture obtained in S4 in a clean bench (protected from light), and adjust the pH to 6.8 again with 1M HCl and 1M NaOH at 37℃. Then add 15g of mung bean powder and 7g of winter melon seed powder, stir and mix well, and continue to culture at 37℃ and 200rpm for 24 hours.

[0074] S6. Filter the culture obtained in step S5 through a 60-mesh sieve, and spray-dry the filtrate that has passed through the sieve. Set the inlet air temperature to 150℃ and the outlet air temperature to 60-80℃. This yields a composition with whitening and freckle-removing effects.

[0075] Effect test We are recruiting individuals aged 25-50 with no underlying medical conditions (including a history of hypertension, hyperlipidemia, diabetes, hyperuricemia, enteritis, gastritis, kidney disease, heart disease, hepatitis, cirrhosis, albinism, vitiligo) or malignant tumors, and a body mass index (BMI) of 18.5-25 kg / m². 2 Forty female volunteers with dull skin tone (confirmed upon inquiry that the volunteers were not born with brown skin) and / or had freckles were randomly divided into four groups. Random adjustments were made to ensure that the age distribution, body mass index distribution, and proportion of smokers and night owls in each group were as consistent / even as possible.

[0076] Before the trial began, the composition of the experimental composition, preparation method, and reagents used were explained in detail to each group of volunteers, and the safety of the product was theoretically explained. Volunteers who could not accept this explanation were replaced. Volunteers who agreed to participate in the trial signed an informed consent form.

[0077] One group of volunteers was randomly selected to consume the composition prepared in Example 1, one group consumed the composition prepared in Example 2, one group consumed the composition prepared in Comparative Example 1, and the remaining group consumed the composition prepared in Comparative Example 2. These groups were designated as Example 1 group, Example 2 group, Comparative Example 1 group, and Comparative Example 2 group, respectively. Volunteers in each group consumed the composition between 20:00 and 20:30, at a dosage of 6g per 50kg of body weight. The composition was added to milk or warm water at a temperature that was not hot to the mouth (approximately 37-42℃). The composition was consumed once daily. During the experiment, volunteers avoided spicy and irritating foods, did not change their skincare products, and maintained their usual daily routines and lifestyle habits for 30 days. If any volunteer experienced itching, rash, or other discomfort, the experiment was immediately stopped, and the time of onset of the symptoms was reported.

[0078] On the first day of the experiment (day 0, before consuming the experimental composition) and the second day after the experiment (day 31), the melanin content (MI) of each group of volunteers was measured using a skin melanin analyzer. A higher MI value indicated a higher melanin content. Facial photographs were taken, and the percentage of facial pigmentation area was calculated. Then, Formula I was used to calculate the percentage change in the percentage of facial pigmentation area.

[0079] Change rate of facial skin pigmentation area percentage (%) = (facial skin pigmentation area before consumption - facial skin pigmentation area after consumption) / facial skin pigmentation area before consumption × 100% (Formula I).

[0080] The statistical results are shown in Table 1 and Figure 1 As shown, none of the volunteers experienced allergic reactions such as itching or rashes. During the trial, two volunteers in Example 1 did not consume the composition for 1 day and 2 days respectively, and did not consume it again. Two volunteers in Comparative Example 2 did not consume the composition for 3 days and 5 days respectively due to work travel, and no results were measured on day 31.

[0081] As can be seen from Table 1, the compositions prepared in Examples 1, 2, Comparative Example 1 and Comparative Example 2 all have certain whitening and freckle-removing effects. Among them, the whitening and freckle-removing effect was the best in the volunteers in Example 1 group. Moreover, the volunteers' age, body mass index, and lifestyle habits such as staying up late and smoking had little impact on the whitening and freckle-removing effect.

[0082] The volunteers in Comparative Example 2 showed the worst results, indicating that the aerobic + anaerobic static fermentation step of Angelica dahurica powder, Polygonatum odoratum powder, and shiitake mushroom powder has a significant impact on the whitening and freckle-removing effects. The results of Example 2 and Comparative Example 1 differed greatly from those of Example 1, suggesting that too short aerobic + anaerobic static fermentation time for Angelica dahurica powder, Polygonatum odoratum powder, and shiitake mushroom powder also resulted in poor effects; fermenting each component in batches yielded better results.

[0083] Table 1. Statistical results of changes in MI value and the percentage of facial pigmentation area.

[0084] Note: ** indicates comparison with Example 2, Comparative Example 1, and Comparative Example 2. P <0.01.

[0085] Therefore, the composition provided by this invention reduces the body's oxidative stress level, providing a suitable internal microenvironment for the efficacy of various drugs and the repair of damage; it provides a sufficient, diverse, and easily absorbed material basis for skin damage repair; it significantly improves the whitening and spot-removing effect by increasing the activity, purity, and content of substances that directly have whitening and spot-removing effects; and it promotes the excretion of metabolic waste by improving gastrointestinal health; it is extremely safe, requires low equipment, and is environmentally friendly, and can be used for daily skin repair and whitening / spot-removing purposes.

[0086] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A composition having whitening and freckle-removing effects, characterized in that, The composition comprises the following ingredients in parts by weight: 5-10 parts Angelica dahurica, 10-20 parts Polygonatum odoratum, 10-20 parts mung bean powder, 5-10 parts winter melon seed kernels and 10-20 parts shiitake mushroom powder; The preparation method of the composition with whitening and freckle-removing effects includes the following steps: S1. Grind Angelica dahurica, Polygonatum odoratum, and winter melon seeds into powder, and pass them through a 60-80 mesh sieve to obtain Angelica dahurica powder, Polygonatum odoratum powder, and winter melon seed powder, respectively. S2. Add angelica powder, polygonatum powder and shiitake powder to an opaque bottle, then add sterile drinking water, stir and mix well, let stand at room temperature, then incubate at 18-22℃ and 180-200rpm for 24-72 hours, then seal with a sterile air-impermeable membrane and let stand. S3. After adjusting the pH of the mixture obtained in S2, add Bacillus subtilis and incubate at 180-200 rpm for 24-72 hours. Then adjust the pH again, add mung bean powder and winter melon seed powder, mix well and incubate at 180-200 rpm for 24-48 hours. S4. Filter the culture obtained in S3, and spray-dry the filtrate.

2. The composition with whitening and freckle-removing effects according to claim 1, characterized in that: The mung bean powder is obtained by grinding fresh, dried mung beans and passing them through a 60-80 mesh sieve; the Solomon's seal is harvested in autumn; the shiitake mushroom powder is obtained by removing impurities from fresh shiitake mushrooms, freeze-drying them, pulverizing them, and then passing them through a 60-80 mesh sieve.

3. The composition with whitening and freckle-removing effects according to claim 2, characterized in that, The composition consists of the following ingredients in parts by weight: 8 parts Angelica dahurica, 12 parts Polygonatum odoratum, 15 parts mung bean powder, 7 parts winter melon seed kernels, and 15 parts shiitake mushroom powder.

4. The composition with whitening and freckle-removing effects according to claim 2, characterized in that, The composition consists of the following ingredients in parts by weight: 5 parts Angelica dahurica, 10 parts Polygonatum odoratum, 10 parts mung bean powder, 5 parts winter melon seeds, and 10 parts shiitake mushroom powder.

5. The composition with whitening and freckle-removing effects according to claim 2, characterized in that, The composition consists of the following ingredients in parts by weight: 10 parts Angelica dahurica, 20 parts Polygonatum odoratum, 20 parts mung bean powder, 10 parts winter melon seeds, and 20 parts shiitake mushroom powder.

6. The composition with whitening and freckle-removing effects according to claim 1, characterized in that: The amount of sterile drinking water added in S2 is 10-15 times the total mass of Angelica dahurica powder, Polygonatum odoratum powder, mung bean powder, winter melon seed powder and shiitake mushroom powder.

7. The composition with whitening and freckle-removing effects according to claim 1, characterized in that: After adding sterile drinking water to S2 and mixing well, let it stand at room temperature for 30-60 minutes, mixing once during the process; then seal it with a sterile, airtight membrane and let it stand at 18-22℃ for 36-72 hours.

8. The composition with whitening and freckle-removing effects according to claim 1, characterized in that: In step S3, pH adjustment is performed using 1M HCl and 1M NaOH to adjust the pH to 6.5-7.0; in step S4, the inlet air temperature for spray drying is 130-200℃, and the outlet air temperature is 60-80℃.

9. The use of a composition having whitening and freckle-removing effects as described in any one of claims 1-8 in a health food product.

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